LOCUS       62056_7960       10441 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        80..459
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        460..663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             746..4849
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4886..4912
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     promoter        4934..5263
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5327..6034
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    6053..6639
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6662..7462
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    7597..7730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             7869..8726
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8900..9488
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9776..9797
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9812..9842
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        9910..9928
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        10089..10329
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        10358..10433
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agacacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
       61 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      121 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      181 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      241 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      301 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      361 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      421 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      481 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      541 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      601 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      661 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
      721 tatagggaga cccaagcttg ccaccatgga caagaagtac agcatcggcc tggacatcgg
      781 taccaacagc gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaagtt
      841 caaggtgctg ggcaacaccg accgccacag catcaagaag aacctgatcg gcgccctgct
      901 gttcgacagc ggcgagaccg ccgaggccac ccgcctgaag cgcaccgccc gccgccgcta
      961 cacccgccgc aagaaccgca tctgctacct gcaggagatc ttcagcaacg agatggccaa
     1021 ggtggacgac agcttcttcc accgcctgga ggagagcttc ctggtggagg aggacaagaa
     1081 gcacgagcgc caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
     1141 ccccaccatc taccacctgc gcaagaagct ggtggacagc accgacaagg ccgacctgcg
     1201 cctgatctac ctggccctgg cccacatgat caagttccgc ggccacttcc tgatcgaggg
     1261 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     1321 caaccagctg ttcgaggaga accccatcaa cgccagcggc gtggacgcca aggccatcct
     1381 gagcgcccgc ctgagcaaga gccgccgcct ggagaacctg atcgcccagc tgcccggcga
     1441 gaagaagaac ggcctgttcg gcaacctgat cgccctgagc ctgggcctga cccccaactt
     1501 caagagcaac ttcgacctgg ccgaggacgc caagctgcag ctgagcaagg acacctacga
     1561 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgttcctggc
     1621 cgccaagaac ctgagcgacg ccatcctgct gagcgacatc ctgcgcgtga acaccgagat
     1681 caccaaggcc cccctgagcg ccagcatgat caagcgctac gacgagcacc accaggacct
     1741 gaccctgctg aaggccctgg tgcgccagca gctgcccgag aagtacaagg agatcttctt
     1801 cgaccagagc aagaacggct acgccggcta catcgacggc ggcgccagcc aggaggagtt
     1861 ctacaagttc atcaagccca tcctggagaa gatggacggc accgaggagc tgctggtgaa
     1921 gctgaaccgc gaggacctgc tgcgcaagca gcgcaccttc gacaacggca gcatccccca
     1981 ccagatccac ctgggcgagc tgcacgccat cctgcgccgc caggaggact tctacccctt
     2041 cctgaaggac aaccgcgaga agatcgagaa gatcctgacc ttccgcatcc cctactacgt
     2101 gggccccctg gcccgcggca acagccgctt cgcctggatg acccgcaaga gcgaggagac
     2161 catcaccccc tggaacttcg aggaggtggt ggacaagggc gccagcgccc agagcttcat
     2221 cgagcgcatg accaacttcg acaagaacct gcccaacgag aaggtgctgc ccaagcacag
     2281 cctgctgtac gagtacttca ccgtgtacaa cgagctgacc aaggtgaagt acgtgaccga
     2341 gggcatgcgc aagcccgcct tcctgagcgg cgagcagaag aaggccatcg tggacctgct
     2401 gttcaagacc aaccgcaagg tgaccgtgaa gcagctgaag gaggactact tcaagaagat
     2461 cgagtgcttc gacagcgtgg agatcagcgg cgtggaggac cgcttcaacg ccagcctggg
     2521 cacctaccac gacctgctga agatcatcaa ggacaaggac ttcctggaca acgaggagaa
     2581 cgaggacatc ctggaggaca tcgtgctgac cctgaccctg ttcgaggacc gcgagatgat
     2641 cgaggagcgc ctgaagacct acgcccacct gttcgacgac aaggtgatga agcagctgaa
     2701 gcgccgccgc tacaccggct ggggccgcct gagccgcaag cttatcaacg gcatccgcga
     2761 caagcagagc ggcaagacca tcctggactt cctgaagagc gacggcttcg ccaaccgcaa
     2821 cttcatgcag ctgatccacg acgacagcct gaccttcaag gaggacatcc agaaggccca
     2881 ggtgagcggc cagggcgaca gcctgcacga gcacatcgcc aacctggccg gcagccccgc
     2941 catcaagaag ggcatcctgc agaccgtgaa ggtggtggac gagctggtga aggtgatggg
     3001 ccgccacaag cccgagaaca tcgtgatcga gatggcccgc gagaaccaga ccacccagaa
     3061 gggccagaag aacagccgcg agcgcatgaa gcgcatcgag gagggcatca aggagctggg
     3121 cagccagatc ctgaaggagc accccgtgga gaacacccag ctgcagaacg agaagctgta
     3181 cctgtactac ctgcagaacg gccgcgacat gtacgtggac caggagctgg acatcaaccg
     3241 cctgagcgac tacgacgtgg accacatcgt gccccagagc ttcctgaagg acgacagcat
     3301 cgacaacaag gtgctgaccc gcagcgacaa gaaccgcggc aagagcgaca acgtgcccag
     3361 cgaggaggtg gtgaagaaga tgaagaacta ctggcgccag ctgctgaacg ccaagctgat
     3421 cacccagcgc aagttcgaca acctgaccaa ggccgagcgc ggcggcctga gcgagctgga
     3481 caaggccggc ttcatcaagc gccagctggt ggagacccgc cagatcacca agcacgtggc
     3541 ccagatcctg gacagccgca tgaacaccaa gtacgacgag aacgacaagc tgatccgcga
     3601 ggtgaaggtg atcaccctga agagcaagct ggtgagcgac ttccgcaagg acttccagtt
     3661 ctacaaggtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     3721 ggtgggcacc gccctgatca agaagtaccc caagctggag agcgagttcg tgtacggcga
     3781 ctacaaggtg tacgacgtgc gcaagatgat cgccaagagc gagcaggaga tcggcaaggc
     3841 caccgccaag tacttcttct acagcaacat catgaacttc ttcaagaccg agatcaccct
     3901 ggccaacggc gagatccgca agcgccccct gatcgagacc aacggcgaga ccggcgagat
     3961 cgtgtgggac aagggccgcg acttcgccac cgtgcgcaag gtgctgagca tgccccaggt
     4021 gaacatcgtg aagaagaccg aggtgcagac cggcggcttc agcaaggaga gcatcctgcc
     4081 caagcgcaac agcgacaagc tgatcgcccg caagaaggac tgggacccca agaagtacgg
     4141 cggcttcgac agccccaccg tggcctacag cgtgctggtg gtggccaagg tggagaaggg
     4201 caagagcaag aagctgaaga gcgtgaagga gctgctgggc atcaccatca tggagcgcag
     4261 cagcttcgag aagaacccca tcgacttcct ggaggccaag ggctacaagg aggtgaagaa
     4321 ggacctgatc atcaagctgc ccaagtacag cctgttcgag ctggagaacg gccgcaagcg
     4381 catgctggcc agcgccggcg agctgcagaa gggcaacgag ctggccctgc ccagcaagta
     4441 cgtgaacttc ctgtacctgg ccagccacta cgagaagctg aagggcagcc ccgaggacaa
     4501 cgagcagaag cagctgttcg tggagcagca caagcactac ctggacgaga tcatcgagca
     4561 gatcagcgag ttcagcaagc gcgtgatcct ggccgacgcc aacctggaca aggtgctgag
     4621 cgcctacaac aagcaccgcg acaagcccat ccgcgagcag gccgagaaca tcatccacct
     4681 gttcaccctg accaacctgg gcgcccccgc cgccttcaag tacttcgaca ccaccatcga
     4741 ccgcaagcgc tacaccagca ccaaggaggt gctggacgcc accctgatcc accagagcat
     4801 caccggtctg tacgagaccc gcatcgacct gagccagctg ggcggcgacg gcggctccgg
     4861 acctccaaag aaaaagagaa aagtataccc ctacgacgtg cccgactacg cctaataact
     4921 cgagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4981 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5041 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5101 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5161 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     5221 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     5281 tatatccatt ttcggatctg atcggcgcgc cataccggtc gccaccatgg tgagcaaggg
     5341 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     5401 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     5461 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     5521 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     5581 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     5641 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     5701 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     5761 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     5821 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     5881 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     5941 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     6001 ccactccacc ggcggcatgg acgagctgta caagtaggcg gccgcgatcc cgcccctctc
     6061 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     6121 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     6181 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     6241 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     6301 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     6361 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     6421 gttggatagt tgtggaaaga gtcaaatggc tcacctcaag cgtattcaac aaggggctga
     6481 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     6541 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     6601 ttttcctttg aaaaacacga tgataagctt gccacaaccc gcgataattc ctgcagccaa
     6661 tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6721 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6781 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6841 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6901 cgcagcagtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6961 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     7021 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     7081 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7141 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     7201 tatgcccgac ggcgatgatc tcgtcgtgac tcatggcgat gcctgcttgc cgaatatcat
     7261 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     7321 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     7381 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     7441 tcgccttctt gacgagttct tctgaacgcg gtgctacgag atttcgattc caccgccgcc
     7501 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7561 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7621 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7681 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7741 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7801 ctcacaattc cacacaacat acgagccgga agcataaagg taagcctgaa tattgaaaaa
     7861 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7921 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7981 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8041 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8101 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8161 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8221 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8281 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     8341 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8401 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     8461 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8521 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8581 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8641 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8701 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8761 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8941 caaaaaaacc accgctacca gcggtggttt ttttgccgga tcaagagcta ccaactcttt
     9001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9121 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9181 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9241 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9301 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9361 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9421 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9481 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9541 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     9601 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     9661 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     9721 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9781 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     9841 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
     9901 attgcaacga tttaggtgac actatagaag agaaggaatt aatacgactc actataggga
     9961 gagagagaga attaccctca ctaaagggag gagaagcatg aattccccag tggaaagacg
    10021 cgcaggcaaa acgcaccacg tgacggagcg tgaccgcgcg ccgagcgcgc gccaaggtcg
    10081 ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
    10141 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
    10201 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
    10261 actatcatat gcttaccgta acttgaaagt atttcgattt cttgggttta tatatcttgt
    10321 ggaaaggacg aggatccgga gacgaagctt cgtctctgtt ttagagctag aaatagcaag
    10381 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttc
    10441 t
//
