LOCUS       V008167                11265 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008167
VERSION     V008167
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Tong Y, Charusanti P, Zhang L, Weber T, Lee SY.
  TITLE     CRISPR-Cas9 based engineering of actinomycetal genomes
  JOURNAL   ACS Synth Biol (2015) In press
   PUBMED   25806970
REFERENCE   2  (bases 1 to 11265)
  AUTHORS   Tong Y, Charusanti P, Zhang L, Weber T, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2015) The Novo Nordisk Foundation Center for
            Biosustainability, Kogle Alle 6, Horsholm 2970, Denmark
REFERENCE   3  (bases 1 to 11265)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11265)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2015) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-MAR-2015) The Novo Nordisk Foundation Center for
            Biosustainability, Kogle Alle 6, Horsholm 2970, Denmark"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(467..576)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             892..1692
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     rep_origin      1994..2582
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2870..2891
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2906..2936
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2944..2960
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2968..2984
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_RNA        3123..3198
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      complement(3368..3402)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             3570..7673
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     terminator      complement(7863..7911)
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             8255..9061
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     CDS             9627..11066
                     /label="pSG5 Rep"
                     /note="replication initiator protein from the Streptomyces
                     ghanaensis plasmid pSG5"
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgaacccg tgccgggctg cggcggatcg cggcagcagg agctccccgc
      181 ccgccacggc tggctggatg gtcgcctcac gcgcccgcag gggtgacggc gtgggtgcgg
      241 cggtcagccc cgtgatcggg cgtcagacgg ccgctgaggg accgcgacca cccctccctt
      301 cccgcctgcc gtctttcacc gcccggccgg tccgcagacg ccgtacggcc aggccacgga
      361 ggattacggg cagatggcgc ccacccggca cccgggctgg ggcaccctgg acagcacgaa
      421 ggcccgcccc actcaccggg gcgggccttc gcgttgagct agagttcccg ccagcctcgc
      481 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
      541 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
      601 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
      661 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa tgcagctcac
      721 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
      781 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
      841 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
      901 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
      961 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
     1021 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctgc gctgggtccg
     1081 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
     1141 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
     1201 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
     1261 atctctgatc cattgcccct gccacctcac tcgcctgcaa gcccggtcgc ccgtgtccat
     1321 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
     1381 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
     1441 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
     1501 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
     1561 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
     1621 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
     1681 cagtcgattg gctgagctca taagttccta ttccgaagtt cctattcgaa atgaccgacc
     1741 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     1801 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     1861 tgctggagtt cttcgcccac cccaaaagga tctaggtgaa gatccttttt gataatctca
     1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattgt
     3001 acgcggtcga tcttgacggc tggcgagagg tgcggggagg atctgaccga cgcggtccac
     3061 acgtggcacc gcgatgctgt tgtgggcaca atcgtgccgg ttggtaggat cgacggccat
     3121 gggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     3181 gtggcaccga gtcggtgctt tttttacgta ggcgtcagct acgatgttcc ggggactgct
     3241 gatccggtca gcaggtggaa gagggactgg attccaaagt tctcaatgct gcttgctgtt
     3301 cttgaatggg gggtcgttga cgacgacatg gctcgattgg cgcgacaagt tgctgcgatt
     3361 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     3421 tgaggtcatt actggaccgg atcggggatc tgggctgagg gagccgacgg cacgcggcgg
     3481 ctcacggcgt ggcacgcgga acgtccgggc ttgcacctca cgtcacgtga ggaggcagcg
     3541 tggacggcgt cagagaaggg agcggacata tggacaagaa gtactccatc ggcctcgaca
     3601 tcggcaccaa ctccgtgggc tgggcggtca tcaccgacga gtacaaggtc ccctccaaga
     3661 agttcaaggt cctgggcaac accgaccggc actcgatcaa gaagaacctg atcggcgccc
     3721 tgctcttcga cagcggcgag accgccgagg cgacccgcct gaagcggacc gcgcgtcgcc
     3781 gctacacccg gcgcaagaac cgcatctgct acctgcagga aatcttctcc aacgagatgg
     3841 ccaaggtgga cgactcgttc ttccaccgcc tggaggagag cttcctggtg gaggaggaca
     3901 agaagcacga gcgccacccg atcttcggca acatcgtgga cgaggtggcc taccacgaga
     3961 agtaccccac catctaccac ctccgcaaga agctggtgga ctcgaccgac aaggcggacc
     4021 tgcggctcat ctacctggcc ctcgcgcaca tgatcaagtt ccgcggccac ttcctcatcg
     4081 agggcgacct gaacccggac aactccgacg tggacaagct cttcatccag ctggtgcaga
     4141 cctacaacca gctgttcgag gagaacccca tcaacgccag cggcgtggac gccaaggcga
     4201 tcctctccgc gcgcctgagc aagtcccggc gcctggagaa cctcatcgcc cagctgccgg
     4261 gcgagaagaa gaacggcctc ttcggcaacc tgatcgcgct gtcgctcggc ctgaccccca
     4321 acttcaagag caacttcgac ctggccgagg acgcgaagct ccagctgtcc aaggacacct
     4381 acgacgacga cctggacaac ctgctcgccc agatcggcga ccagtacgcg gacctcttcc
     4441 tggccgcgaa gaacctctcg gacgccatcc tgctcagcga catcctgcgg gtcaacaccg
     4501 agatcaccaa ggccccgctg tcggcgagca tgatcaagcg gtacgacgag caccaccagg
     4561 acctgaccct gctcaaggcc ctcgtgcgcc agcagctgcc cgagaagtac aaggaaatct
     4621 tcttcgacca gtccaagaac ggctacgccg gctacatcga cggcggcgcg tcgcaggagg
     4681 agttctacaa gttcatcaag ccgatcctgg agaagatgga cggcaccgag gagctgctcg
     4741 tcaagctgaa ccgcgaggac ctgctccgca agcagcggac cttcgacaac ggctccatcc
     4801 cgcaccagat ccacctgggc gagctccacg ccatcctccg gcgccaggag gacttctacc
     4861 ccttcctgaa ggacaaccgc gagaagatcg agaagatcct gaccttccgc atcccgtact
     4921 acgtcggccc cctggcccgc ggcaactccc ggttcgcgtg gatgacccgg aagtcggagg
     4981 agaccatcac cccgtggaac ttcgaggagg tcgtggacaa gggcgcgtcc gcgcagtcgt
     5041 tcatcgagcg catgaccaac ttcgacaaga acctcccgaa cgagaaggtc ctgcccaagc
     5101 actccctgct ctacgagtac ttcaccgtgt acaacgagct gaccaaggtc aagtacgtga
     5161 ccgagggcat gcggaagccg gccttcctgt cgggcgagca gaagaaggcg atcgtggacc
     5221 tgctcttcaa gaccaaccgc aaggtcaccg tgaagcagct gaaggaggac tacttcaaga
     5281 agatcgagtg cttcgactcc gtcgagatca gcggcgtgga ggaccgcttc aacgcctccc
     5341 tgggcaccta ccacgacctg ctcaagatca tcaaggacaa ggacttcctc gacaacgagg
     5401 agaacgagga catcctggag gacatcgtcc tcaccctgac cctcttcgag gaccgcgaga
     5461 tgatcgagga gcggctcaag acctacgccc acctgttcga cgacaaggtg atgaagcagc
     5521 tgaagcgtcg ccgctacacc ggctggggcc gcctctcccg gaagctgatc aacggcatcc
     5581 gggacaagca gagcggcaag accatcctgg acttcctcaa gtccgacggc ttcgccaacc
     5641 gcaacttcat gcagctcatc cacgacgaca gcctgacctt caaggaggac atccagaagg
     5701 cccaggtctc gggccagggc gacagcctcc acgagcacat cgccaacctg gcgggctccc
     5761 cggcgatcaa gaagggcatc ctccagaccg tcaaggtcgt ggacgagctg gtcaaggtga
     5821 tgggccgcca caagcccgag aacatcgtga tcgagatggc ccgggagaac cagaccaccc
     5881 agaagggcca gaagaactcg cgcgagcgga tgaagcggat cgaggagggc atcaaggagc
     5941 tcggcagcca gatcctgaag gagcacccgg tcgagaacac ccagctgcag aacgagaagc
     6001 tgtacctcta ctacctgcag aacggccgcg acatgtacgt ggaccaggag ctcgacatca
     6061 accggctgtc cgactacgac gtggaccaca tcgtgccgca gtccttcctg aaggacgact
     6121 cgatcgacaa caaggtcctg acccgctcgg acaagaaccg gggcaagtcc gacaacgtgc
     6181 cctcggagga ggtcgtgaag aagatgaaga actactggcg ccagctgctc aacgccaagc
     6241 tcatcaccca gcgcaagttc gacaacctga ccaaggccga gcggggcggc ctgagcgagc
     6301 tcgacaaggc gggcttcatc aagcgccagc tggtcgagac ccggcagatc accaagcacg
     6361 tggcccagat cctggactcc cggatgaaca ccaagtacga cgagaacgac aagctgatcc
     6421 gcgaggtcaa ggtgatcacc ctcaagagca agctggtctc cgacttccgc aaggacttcc
     6481 agttctacaa ggtccgggag atcaacaact accaccacgc ccacgacgcg tacctgaacg
     6541 ccgtcgtggg caccgcgctg atcaagaagt acccgaagct ggagtccgag ttcgtctacg
     6601 gcgactacaa ggtctacgac gtgcgcaaga tgatcgccaa gagcgagcag gagatcggca
     6661 aggccaccgc gaagtacttc ttctactcca acatcatgaa cttcttcaag accgagatca
     6721 ccctggccaa cggcgagatc cgcaagcggc ccctgatcga gaccaacggc gagaccggcg
     6781 agatcgtctg ggacaagggc cgcgacttcg ccaccgtccg gaaggtgctg tcgatgccgc
     6841 aggtcaacat cgtgaagaag accgaggtgc agaccggcgg cttcagcaag gagtccatcc
     6901 tccccaagcg caacagcgac aagctgatcg cccggaagaa ggactgggac ccgaagaagt
     6961 acggcggctt cgacagcccc accgtcgcct actccgtgct ggtcgtggcg aaggtcgaga
     7021 agggcaagag caagaagctg aagtccgtga aggagctgct cggcatcacc atcatggagc
     7081 gctcctcgtt cgagaagaac ccgatcgact tcctggaggc caagggctac aaggaggtca
     7141 agaaggacct catcatcaag ctgcccaagt acagcctgtt cgagctggag aacggccgca
     7201 agcggatgct cgcctccgcg ggcgagctgc agaagggcaa cgagctggcc ctcccgtcga
     7261 agtacgtcaa cttcctgtac ctcgcgtccc actacgagaa gctgaagggc tcgcccgagg
     7321 acaacgagca gaagcagctc ttcgtggagc agcacaagca ctacctggac gagatcatcg
     7381 agcagatcag cgagttcagc aagcgcgtca tcctggccga cgcgaacctc gacaaggtgc
     7441 tgtccgccta caacaagcac cgcgacaagc cgatccggga gcaggcggag aacatcatcc
     7501 acctgttcac cctcaccaac ctgggcgccc ccgccgcgtt caagtacttc gacaccacca
     7561 tcgaccgcaa gcggtacacc tccaccaagg aggtcctcga cgcgaccctg atccaccaga
     7621 gcatcaccgg cctgtacgag acccgcatcg acctgtccca gctcggcggc gactgaaagc
     7681 tttctagagg atccccgggt accgagctcg aattccccag atctaaagtt ttgtcgtctt
     7741 tccagacgtt agtaaatgaa ttttctgtat gaggttttgc taaacaactt tcaacagttt
     7801 cagcggagtg agaatagaaa ggaacaacta aaggaattgc gaataataat tttttcacgt
     7861 tgaaaatctc caaaaaaaaa ggctccaaaa ggagccttta attgtatcgg tttatcagct
     7921 tgctttcgag gtgaatttct taaacagctt gataccgata gttgcgccga caatgacaac
     7981 aaccatcgcc cacgcataac cgatatattc ggtcgctgag gcttgcaggg agtcaaaggc
     8041 cgcttttgcg ggatctcgtc gaaggcacta gaaggcctat atgcggggat cgaccgcgcg
     8101 ggtcccggac ggggaagagc ggggagcttt gccagagagc gacgacttcc ccttgcgttg
     8161 gtgattgccg gtcagggcag ccatccgcca tcgtcgcgta gggtgtcaca ccccaggaat
     8221 cgcgtcactg aacacagcag ccggtaggac gaccatgact gagttggaca ccatcgcaaa
     8281 tccgtccgat cccgcggtgc agcggatcat cgatgtcacc aagccgtcgc gatccaacat
     8341 aaagacaacg ttgatcgagg acgtcgagcc cctcatgcac agcatcgcgg ccggggtgga
     8401 gttcatcgag gtctacggca gcgacagcag tccttttcca tctgagttgc tggatctgtg
     8461 cgggcggcag aacataccgg tccgcctcat cgactcctcg atcgtcaacc agttgttcaa
     8521 gggggagcgg aaggccaaga cattcggcat cgcccgcgtc cctcgcccgg ccaggttcgg
     8581 cgatatcgcg agccggcgtg gggacgtcgt cgttctcgac ggggtgaaga tcgtcgggaa
     8641 catcggcgcg atagtacgca cgtcgctcgc gctcggagcg tcggggatca tcctggtcga
     8701 cagtgacatc accagcatcg cggaccggcg tctccaaagg gccagccgag gttacgtctt
     8761 ctcccttccc gtcgttctct ccggtcgcga ggaggccatc gccttcattc gggacagcgg
     8821 tatgcagctg atgacgctca aggcggatgg cgacatttcc gtgaaggaac tcggggacaa
     8881 tccggatcgg ctggccttgc tgttcggcag cgaaaagggt gggccttccg acctgttcga
     8941 ggaggcgtct tccgcctcgg tttccatccc catgatgagc cagaccgagt ctctcaacgt
     9001 ttccgtttcc ctcggaatcg cgctgcacga gaggatcgac aggaatctcg cggccaaccg
     9061 ataagcgcct ctgttcctcg gacgctcggt tcctcgacct cgattcgtca gtgatgatca
     9121 acaggaccca gaaatggcac gagcccggga ttggcctccc gggctcgccg cccccgaacc
     9181 ggcgcagcat gccgagtgag gacgtttgct ctgtgacgag cgtacgagag aacagcgcgt
     9241 cgacaccacc gcgccccgcc ggaccgaagt gccactgcgg caccccgatc gtccacatcc
     9301 ccgggaagcg ccccaaaatc tactgctcgg acgcctgccg aaagcgcgca aagcgtctca
     9361 ttgcggacca gtcacgcagc gtagcaagca cgactacagc gggtaatccg gacgcgggct
     9421 ttagtagtaa gggaaaatct ccgtccactg caggtcagag cgctgtaact ggaacgggtt
     9481 ctaccccaaa cgagggtgcc gcgacagccg ggcgggagga cccgcgcgac gagcggttcg
     9541 cccggcggga ccggcaccag acggtgtcgc tgaacgaggc gttcaagggc tgcgggaccc
     9601 ggctcactgc cgggacggcc gagctgatgt ggaagccggg cgaggcgacg tggggcaaca
     9661 cgtgccgctg caacaacgtc cacacctgcc cgtggtgcat gagccgcatc ctggccgtac
     9721 ggggctcgaa cgtgcagctc gccgccgacg gtctcgcgga cgccgggtac gggctgcacc
     9781 ttggcacgaa cacgttgcgc cacttcgagc gcatggcgtt cggcaccgtc cgcaagggaa
     9841 tgcggcacgg cctggtcgcg gtcttgcacg acggctggaa gggcgcgtac ggctcgtccg
     9901 gccgccgctg gcgcacgatg cgcgacgact tcgggatcat cggctacgag cgggccttcg
     9961 aggacacctt cggctggggc tccggctggc acctgcactg gcacacgctc tgggtcaccc
    10021 gcgaggtgct cggcccggac gcccaggccg cgttccgcga cgcactcgcc ggagcctggg
    10081 ccgccggggt cgaatccgcg ggcggctaca ccgtcagcga gacgtgcgac cggcccggtt
    10141 gctcctgtga gggcaagggc cacggcaccg acgtgcgccc actcaacggc gcggacgccg
    10201 ctgacggcga tgcgggcaag caggcccgct acctctacaa ggacggcgac aagaccaagg
    10261 gcggcgtcgc caagatcggc ctggagctgg ccggacagaa cttcaaggcc ggtcgcggcg
    10321 acgaccgcat gggaccgctc gacctgggcg acgcggcggc cgccgagctg cagcggcttc
    10381 gtcggcccgg cccgttcgtc gagaagtacc gcgagcgcga gttcggcgtc ttccaggtcc
    10441 gcaagcacta ccggtcgcag aacctgaacc ggctgatcaa ggaactcggc atccagcagg
    10501 acgtccgcac cgaggaggag atcaccgacg acaccgaggg cctggtcgcc atcgcggtca
    10561 tcccggccta catctggtac cgatacatcg cccgcgtcgc cggacgtcgc ctcgacctca
    10621 tcaaggtcgc cgagacgtac ggcctaccgg gcgtccggcg gctcgtcgag tcctggggcc
    10681 tggtgtgggg caaggacgtc ctggacccgc ccgccccgga agcgccggcc gcgccgggcg
    10741 accttgacgc tgaccagatg aggttcgagg tcatgtccga ggaggaagct gctttccgcg
    10801 aggcgcgccg gaaggccaac gaggcccgca cggaggagct ggccgcttcg ctcgaccgag
    10861 tgcgtcagcc gaagaaggag gcgatccgcc cgacgatcag ccttcggaag cgcctgaagc
    10921 cgaagccggt caccgtcgac gtcaagactc ccccgcccgg cgccgcgtcg cccgtgtgcc
    10981 gccgctgcaa gggcaagctc gccccggtac tccagccctg gggccggctc cccggagact
    11041 gcctccgcgt cgacacagcg gtcgcgtagg gccgctgagg gaccgccatg agaccgccac
    11101 cccggacatc cgggtggcgg tctcatggcg gtcccggcgc gaaagccgag ggtctgcctg
    11161 ccgtgaggtg gccggcggac ctgggttact gctggaatcc cgcgcccagt ccgagcgtcc
    11221 gccggcgctt ttcacctggc tccgggtgct cgtcgatgag cacca
//
