LOCUS       .                       6130 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        151..182
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             215..1864
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             1868..1987
                     /label="hPEST"
                     /note="PEST degradation sequence from mouse ornithine
                     decarboxylase"
     polyA_signal    complement(2039..2160)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        2354..2711
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2778..3569
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3746..3879
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4224..4812)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5015..5872)
                     /label="AmpR"
                     /note="β-lactamase"
     polyA_signal    5977..6025
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    6039..6130
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcgcaccaga cagtgacgtc agctgccaga
       61 tcccatggcc gtcatactgt gacgtctttc agacacccca ttgacgtcaa tgggagaaca
      121 gatctggcct cggcggccaa gcttagacac tagagggtat ataatggaag ctcgacttcc
      181 agcttggcaa tccggtactg ttggtaaagc caccatggaa gatgccaaaa acattaagaa
      241 gggcccagcg ccattctacc cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc
      301 catgaagcgc tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt
      361 ggacattacc tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg
      421 ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc gagaatagct tgcagttctt
      481 catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta
      541 caacgagcgc gagctgctga acagcatggg catcagccag cccaccgtcg tattcgtgag
      601 caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat
      661 catcatcatg gatagcaaga ccgactacca gggcttccaa agcatgtaca ccttcgtgac
      721 ttcccatttg ccacccggct tcaacgagta cgacttcgtg cccgagagct tcgaccggga
      781 caaaaccatc gccctgatca tgaacagtag tggcagtacc ggattgccca agggcgtagc
      841 cctaccgcac cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa
      901 ccagatcatc cccgacaccg ctatcctcag cgtggtgcca tttcaccacg gcttcggcat
      961 gttcaccacg ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga
     1021 ggaggagcta ttcttgcgca gcttgcaaga ctataagatt caatctgccc tgctggtgcc
     1081 cacactattt agcttcttcg ctaagagcac tctcatcgac aagtacgacc taagcaactt
     1141 gcacgagatc gccagcggcg gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa
     1201 acgcttccac ctaccaggca tccgccaggg ctacggcctg acagaaacaa ccagcgccat
     1261 tctgatcacc cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt
     1321 cgaggctaag gtggtggact tggacaccgg taagacactg ggtgtgaacc agcgcggcga
     1381 gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt aacaaccccg aggctacaaa
     1441 cgctctcatc gacaaggacg gctggctgca cagcggcgac atcgcctact gggacgagga
     1501 cgagcacttc ttcatcgtgg accggctgaa gagcctgatc aaatacaagg gctaccaggt
     1561 agccccagcc gaactggaga gcatcctgct gcaacacccc aacatcttcg acgccggggt
     1621 cgccggcctg cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca
     1681 cggtaaaacc atgaccgaga aggagatcgt ggactatgtg gccagccagg ttacaaccgc
     1741 caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa
     1801 gttggacgcc cgcaagatcc gcgagattct cattaaggcc aagaagggcg gcaagatcgc
     1861 cgtgaattct cacggcttcc ctcccgaggt ggaggagcag gccgccggca ccctgcccat
     1921 gagctgcgcc caggagagcg gcatggatag acaccctgct gcttgcgcca gcgccaggat
     1981 caacgtctaa ggccgcgact ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     2041 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2101 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2161 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2221 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtt tgcgtattgg
     2281 gcgctcttcc gctgatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
     2341 ggttagctac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     2401 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2461 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2521 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     2581 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     2641 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     2701 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     2761 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2821 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2881 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     2941 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3001 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3061 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3121 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3181 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3241 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3301 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3361 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3421 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3481 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3541 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3601 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3661 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3721 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     3781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3841 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     3901 cttggcgtaa tcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     3961 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     4021 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4081 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4141 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4201 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4261 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4321 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4381 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4441 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4501 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4561 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4621 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4681 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4741 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4801 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4861 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4921 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4981 agcggccgca aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga
     5041 tctcagcgat ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca
     5101 ctacgattcg tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc
     5161 gttcaccggc ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa
     5221 gtggtcctgc tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag
     5281 taagaagttc gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg
     5341 tatcacgctc gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg
     5401 tcacatgatc acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg
     5461 tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc
     5521 ttaccgtcat gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt
     5581 tttgtgagta gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca
     5641 ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga
     5701 aagactcaag gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca
     5761 gttgatcttc agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc
     5821 aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc
     5881 tttttcaata ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa
     5941 gtaatattaa ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta
     6001 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
     6061 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
     6121 acatttctct
//
