LOCUS       40924_13355        5914 bp DNA     circular SYN 17-DEC-2018
DEFINITION  C. glutamicum-E. coli shuttle vector pCRB62, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Okibe N, Suzuki N, Inui M, Yukawa H.
  TITLE     Antisense-RNA-mediated plasmid copy number control in pCG1-family 
            plasmids, pCGR2 and pCG1, in Corynebacterium glutamicum
  JOURNAL   Microbiology (Reading, Engl.) 156 (PT 12), 3609-3623 (2010)
  PUBMED    20798162
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Gunji W, Inui M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2010) Molecular Microbiology and Biotechnology 
            Group, Research Institute of Innovative Technology for the Earth 
            (RITE), 9-2 Kizugawadai, Kizugawa, Kyoto 619-0292, Japan
REFERENCE   3  (bases 1 to 5914)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5914)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology (Reading, Engl.) 156 (PT 12), 3609-3623 (2010)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2010) Molecular Microbiology and Biotechnology Group, 
            Research Institute of Innovative Technology for the Earth (RITE), 
            9-2 Kizugawadai, Kizugawa, Kyoto 619-0292, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    265..286
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        301..331
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    339..355
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     363..379
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    389..445
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(449..465)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(791..1379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1592..2191
                     /codon_start=1
                     /gene="parA"
                     /product="ParA"
                     /label=parA
                     /protein_id="ADR57706.1"
                     /translation="MPILTIAHTKGGVGKTTSAVLLCAAAHARGLAVTLIDSDAQGTAT
                     AWAHAAEEAGDTFPWPIITAATPAHLARTLDGHDGLVIVDTPPGGYEVIETAIEAGDLI
                     LIPTSASPLDINRVWPTVEATSHKPAVVCLSQIDSRTTLPKIARTALENEGVVVAETEI
                     PAREALRHMYATTPQRLYGYDELLTELLSSNLLGEH"
     gene            1592..2191
                     /gene="parA"
                     /label=parA
     CDS             2191..2418
                     /codon_start=1
                     /gene="parB"
                     /product="ParB"
                     /label=parB
                     /protein_id="ADR57707.1"
                     /translation="MADLSKKLAKTPVTSAPQKKVAETFTTATEKPTRTTIYLPTSLAK
                     RLKHAAVEEERSVSAILAGLAEDWLNKEDD"
     gene            2191..2418
                     /gene="parB"
                     /label=parB
     CDS             3193..4650
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="pCGR2 RepA"
                     /protein_id="ADR57705.1"
                     /translation="MSLPSKGRSSTPTGVRVPQPLPTYRDTGGLDDTPAGFTDRDALID
                     HLGRKAIHGSKDRDFGKAYYRHEDGTLRPRLYRVDSEALARCQYVMLTTQQYAAVLVVD
                     IDQPGQSGGHPADLSPEVRQRMSALIEHNLGPSWVGINPQNGKAQAIWLIDPVYADKSG
                     KSRHMSLLAATSRALGELLDHDPNFSHRFSRSPFYDGNDPTAYRWYCQHKRVRRLADLL
                     KEIRTMTGQEQYTKPRQQFSSGRELINAVKTRREEAQAFKALAQDVETELSTELDQYDP
                     ELIDGVRVLWISQGRAARDETAFRYALKTCHRLRAAGERMTDAAIIDAYEHAYNVAQRH
                     GGDGRDSEMPPMRDRQTMARRVRGYVTQSKTDMGTSASPGRATSTERKALATMGRRGGK
                     KAAERWKDRDSDYAQSELKKLEHTHRVNKARGISTKSRISQMVNDQYLQTGTIPSWREI
                     ADDLGLSRATVARHVAVLKKSGEYPEF"
     gene            3193..4650
                     /gene="repA"
                     /label=repA
     CDS             complement(4987..5799)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgaggtcga acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttggata
       61 tctaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata
      121 agcggatgaa tggcagaaat tcagcttggc ccagtgccaa gctccaatac gcaaaccgcc
      181 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      241 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      301 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      361 cacaggaaac agctatgacc atgattacga attcgagctc ggtacccggg gatcctctag
      421 agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
      481 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      541 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      601 aatgagcttc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      661 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      841 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      901 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      961 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1021 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1081 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1141 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1201 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1261 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1321 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1381 aagatccttt gatcttttct acggggtctg acgctcagtg gaacccgcgg tcattgctct
     1441 acttcccagc tggatcgact ctgggaaatg tgggctcgaa ttttccattt gcagagcgta
     1501 tgaaacgtta ttgaaaaatt atgtttaaac gctacaacat acgtatgttg tatgttctgc
     1561 agttgtgacg tttgtatgtc atagtgttct tatgccaatc ttgactatcg ctcacacaaa
     1621 gggtggcgta ggaaaaacaa cctctgcggt acttttgtgc gcagctgccc acgctcgcgg
     1681 actagccgtc acgctcattg attccgacgc ccagggcacc gccaccgcct gggcgcacgc
     1741 cgccgaagaa gccggtgata ctttcccatg gcctatcatc acagcagcca cccctgccca
     1801 ccttgcccgc acactcgacg ggcatgacgg actcgttatc gttgataccc cgcctggtgg
     1861 ctacgaagtg atcgagaccg ccattgaagc aggcgatctc atcctgatcc ccacctccgc
     1921 ctccccacta gacatcaacc gagtctggcc gactgttgag gccaccagcc acaagcccgc
     1981 cgtagtctgt ctatctcaga tcgactcccg caccaccttg cccaagatcg cccgtaccgc
     2041 acttgaaaac gaaggcgtcg tcgtcgctga aacggagatc cctgcccggg aagcgttaag
     2101 acatatgtat gctacaactc cacaacgttt atatggctac gatgaactac ttactgaact
     2161 actgtcctca aacctgttgg gagaacatta atggctgatt tatccaagaa gctggcgaaa
     2221 acgcccgtta ccagcgcacc gcaaaagaaa gtggccgaga cctttaccac cgccactgag
     2281 aagcccaccc gcaccaccat ttaccttcca acaagtctcg ctaagcgcct taagcatgct
     2341 gccgtcgaag aggagcgcag tgtctctgca atcctcgcag gcctggccga agactggcta
     2401 aacaaagaag acgactaagt atgtttgtat gtcatggcat acgacataca aacataacaa
     2461 catagaaacc taacaacgta ttaaatccaa gtagtcagcg ctggagacca tgcctcttcg
     2521 acacgcggct gtgctgctcg tggggccata agtggtgcag gactgatgaa agaaacccac
     2581 aagttgttta agcgtcaaag catcaagcgg gcacagcatt aaaacattaa gacgtttaga
     2641 cccttgcggc ctttggcacc agtcctgccg aaacttatac attcgacgca ctctatgaag
     2701 agatttgagg aatgaaggag acggctttaa tgccaccagc acggcgcggt tcgcgaacca
     2761 ccaccggacg aaaaacagca cgcccctcgg cggcaacacc cacgccgacc gacgaggaag
     2821 gcacagagct cacccggcgt acaaccattt acctcaaaaa ggaaacatgg aaatccatga
     2881 agctcatgac catagaaaca ggagaaagcg tttctgcgta catcgaacga ctcattgata
     2941 aagatgtaaa gcgcgtccag aaaaagttac tccaaaaccc caaaacgcta taacacgcag
     3001 acataacaac gttatagtgc tttgacgcta aactgtatcg gggcaggtta aaaacttttc
     3061 gtgtcgcggg cacagagcaa tcacactcgt gttactctgg tcgaaaacct tataaatgca
     3121 tgaagtccgc caggcttgca cccctgacgg acttcgctat cacccggagg acacccgggg
     3181 gaaagcacgt caatgagcct accttcaaaa ggacgatcaa gcacacccac gggtgtgcgt
     3241 gttccccaac cactgcccac ctaccgcgac actggcggcc tggacgacac cccggcagga
     3301 ttcaccgatc gtgatgcact tatagatcat ctcgggcgca aagcaatcca cggaagcaaa
     3361 gaccgtgact tcggcaaagc ttattaccgc cacgaggacg gtactcttcg cccgcgcttg
     3421 tatcgcgtgg attctgaagc gttggcacgc tgccagtacg tcatgctcac cacgcagcaa
     3481 tacgccgctg tattagtggt cgatattgac cagcccggcc aatcgggagg gcacccagca
     3541 gacttatcgc ctgaggttcg tcagcgaatg tcagctttga tcgagcacaa tcttggaccg
     3601 tcatgggtgg gcattaatcc ccaaaacggc aaagcccagg cgatctggtt gatcgatccg
     3661 gtgtacgcag acaaaagcgg caaatcccgg catatgagtc tgcttgccgc gacgagccgt
     3721 gctttgggtg agctgttgga tcatgatccg aacttctcgc accgttttag ccggagcccg
     3781 ttttatgacg gcaacgaccc taccgcctat cgttggtatt gccagcacaa acgcgtgcgc
     3841 cggttagctg atcttcttaa ggagatacgc accatgacgg gtcaagagca gtacaccaag
     3901 cctcggcaac agttttctag tggccgcgag cttattaatg ctgtgaaaac tcgcagagaa
     3961 gaagcccaag catttaaagc acttgcccag gacgtcgaga ccgaactcag cacagagctt
     4021 gatcagtacg acccggaact tatcgacggg gtacgagttt tatggattag ccaggggcgt
     4081 gcggccaggg atgagacggc gtttcgttac gctttgaaaa cctgccaccg gctacgtgcc
     4141 gcaggtgagc gtatgactga tgccgcgatc attgatgcct atgagcatgc gtataacgtt
     4201 gctcagcgcc atgggggaga tggccgggat agtgagatgc caccgatgcg ggatcgccag
     4261 acgatggcgc gtcgtgtgcg tggatacgtg actcaatcta agaccgatat gggcacgtca
     4321 gcgtccccag ggcgcgctac aagcactgaa cgtaaagcgt tagccacgat ggggcgtaga
     4381 ggcggtaaaa aggccgcaga acgctggaaa gatcgcgata gtgattatgc ccaatcagaa
     4441 ttaaagaagc tagaacacac acatcgggtc aacaaagcga gaggaatatc aaccaagtct
     4501 cgcattagcc aaatggttaa tgaccagtac ctacaaactg gaacgatccc ctcatggaga
     4561 gagatagctg acgatcttgg tctatccagg gctaccgttg ctcggcatgt cgcagttttg
     4621 aaaaagagtg gtgagtatcc cgaattctag ggggtctcat ctcgtaagca atatacggtt
     4681 cccccggcgg tagcaggagg ttatctattt caaggtttta agatcttgct cccgatgata
     4741 gagatgagtc aagacgcaca cctcactcgt aatgacttgg gctagtagcc ctacctgcgg
     4801 agtcgactgc tcccggaggg ttttggtgat taattcaaga tcactggtta gagcattact
     4861 actggtctgt agcatctaca gacttggtta gactcagttg gagcaatgaa ggttccacag
     4921 gctcgagtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc
     4981 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     5041 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     5101 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     5161 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     5221 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg
     5281 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     5341 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     5401 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     5461 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     5521 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     5581 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     5641 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     5701 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     5761 cggcttcgag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     5821 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
     5881 tcagagattt tgagacacaa cgtggctttg ttgc
//
