LOCUS       40924_13320        4976 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cre-lox Univector acceptor vector pCR705, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Jia F, Gampala SS, Mittal A, Luo Q, Rock CD.
  TITLE     Cre-lox univector acceptor vectors for functional screening in 
            protoplasts: analysis of Arabidopsis donor cDNAs encoding ABSCISIC 
            ACID INSENSITIVE1-like protein phosphatases
  JOURNAL   Plant Mol. Biol. 70 (6), 693-708 (2009)
  PUBMED    19499346
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Rock CD, Jia F, Gampala SSL., Mittal A, Luo Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2009) Biological Science, Texas Tech University, 
            2500 Broadway, Lubbock, TX 79409, USA
REFERENCE   3  (bases 1 to 4976)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4976)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2009"; volume: "70"; issue: "6"; pages: "693-708"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2009) Biological Science, Texas Tech University, 2500 
            Broadway, Lubbock, TX 79409, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        411..2402
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2427..2456
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             2457..2486
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(2487..2520)
                     /label=loxH
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021). The loxH variant 
                     was optimized for expression constructs but remains 
                     compatible with loxP."
     regulatory      2521..2740
                     /label=Cauliflower mosaic virus (CaMV) 35S terminator
                     /note="Cauliflower mosaic virus (CaMV) 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2755..2771)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2779..2795)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2803..2833)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2848..2869)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3157..3745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3919..4776)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4777..4881)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagtgca
      421 gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa
      481 aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca
      541 tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt
      601 ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga gtattttgac
      661 aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat
      721 agcttcacct atataatact tcatccattt tattagtaca tccatttagg gtttagggtt
      781 aatggttttt atagactaat ttttttagta catctatttt attctatttt agcctctaaa
      841 ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa
      901 taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa
      961 acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac
     1021 ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca
     1081 tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg
     1141 ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc
     1201 ctcctcctcc tctcacggca cggcagctac gggggattcc tttcccaccg ctccttcgct
     1261 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct
     1321 cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac
     1381 ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc
     1441 ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat
     1501 ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag
     1561 acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag
     1621 ccgttccgca gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt
     1681 tgcccttttc ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg
     1741 cttttttttg tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt
     1801 agaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca
     1861 tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat
     1921 acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt
     1981 gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt
     2041 caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata
     2101 gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg
     2161 ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga
     2221 gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg
     2281 gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt
     2341 tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc
     2401 aggtcgactc tagaggatcc aaaatggagc aaaagttgat cagcgaggag gatttggagc
     2461 agaaacttat cagcgaggag gatttgatta cctcatatag catacattat acgaagttat
     2521 gaattcccat gcccgctgaa atcaccagtc tctctctaca aatctatctc tctctataat
     2581 aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt
     2641 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
     2701 tttctaattc ctaaaaccaa aatccagggg taccgagctc aaattcgtaa tcatgtcata
     2761 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2821 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2881 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2941 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3001 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3061 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3121 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3181 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3241 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3301 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3361 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3421 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3481 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3541 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     3601 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3661 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3721 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3781 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3841 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3901 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3961 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4021 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4081 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4141 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4201 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4261 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4321 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4381 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4441 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4501 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4561 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4621 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4681 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4741 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4801 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4861 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     4921 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
