LOCUS       40924_13315        4970 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cre-lox Univector acceptor vector pCR704, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Jia F, Gampala SS, Mittal A, Luo Q, Rock CD.
  TITLE     Cre-lox univector acceptor vectors for functional screening in 
            protoplasts: analysis of Arabidopsis donor cDNAs encoding ABSCISIC 
            ACID INSENSITIVE1-like protein phosphatases
  JOURNAL   Plant Mol. Biol. 70 (6), 693-708 (2009)
  PUBMED    19499346
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Rock CD, Jia F, Gampala SSL., Mittal A, Luo Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2009) Biological Science, Texas Tech University, 
            2500 Broadway, Lubbock, TX 79409, USA
REFERENCE   3  (bases 1 to 4970)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4970)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2009"; volume: "70"; issue: "6"; pages: "693-708"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2009) Biological Science, Texas Tech University, 2500 
            Broadway, Lubbock, TX 79409, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        411..2402
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2427..2450
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             2454..2477
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     protein_bind    complement(2481..2514)
                     /label=loxH
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021). The loxH variant 
                     was optimized for expression constructs but remains 
                     compatible with loxP."
     regulatory      2515..2734
                     /label=Cauliflower mosaic virus (CaMV) 35S terminator
                     /note="Cauliflower mosaic virus (CaMV) 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2749..2765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2773..2789)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2797..2827)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2842..2863)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3151..3739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3913..4770)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4771..4875)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagtgca
      421 gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa
      481 aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca
      541 tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt
      601 ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga gtattttgac
      661 aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat
      721 agcttcacct atataatact tcatccattt tattagtaca tccatttagg gtttagggtt
      781 aatggttttt atagactaat ttttttagta catctatttt attctatttt agcctctaaa
      841 ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa
      901 taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa
      961 acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac
     1021 ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca
     1081 tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg
     1141 ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc
     1201 ctcctcctcc tctcacggca cggcagctac gggggattcc tttcccaccg ctccttcgct
     1261 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct
     1321 cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac
     1381 ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc
     1441 ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat
     1501 ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag
     1561 acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag
     1621 ccgttccgca gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt
     1681 tgcccttttc ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg
     1741 cttttttttg tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt
     1801 agaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca
     1861 tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat
     1921 acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt
     1981 gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt
     2041 caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata
     2101 gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg
     2161 ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga
     2221 gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg
     2281 gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt
     2341 tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc
     2401 aggtcgactc tagaggatcc aaaatggatt acaaggatga tgatgataag ggagattata
     2461 aggacgatga cgataaagga attacctcat atagcataca ttatacgaag ttatgaattc
     2521 ccatgcccgc tgaaatcacc agtctctctc tacaaatcta tctctctcta taataatgtg
     2581 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     2641 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     2701 attcctaaaa ccaaaatcca ggggtaccga gctcaaattc gtaatcatgt catagctgtt
     2761 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2821 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2881 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2941 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3001 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3061 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3121 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3181 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3241 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3301 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3361 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3421 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3481 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3541 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     3601 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3661 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3721 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3781 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3841 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3901 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3961 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4021 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4081 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4141 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4201 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4261 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4321 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4381 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4441 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4501 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4561 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4621 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4681 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4741 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4801 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     4921 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
