LOCUS       V008201                 5843 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008201
VERSION     V008201
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5843)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5843)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        638..656
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             691..723
                     /label="VSV-G tag"
                     /note="epitope tag from vesicular stomatitis virus G
                     protein"
     CDS             736..1542
                     /gene="Il1b"
                     /label="Interleukin-1 beta"
                     /note="Interleukin-1 beta from Mus musculus. Accession#:
                     P10749"
     promoter        complement(1558..1576)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1602..1826
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1958..2546)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(2875..2922)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3157..3942)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(3977..4334)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             complement(4397..5254)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5255..5359)
                     /label="AmpR promoter"
     rep_origin      5386..5841
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccggatc cgccaccatg tacaccgaca tcgagatgaa ccggttgggc
      721 aaggaattgg gtcagatggc aactgttcct gaactcaact gtgaaatgcc accttttgac
      781 agtgatgaga atgacctgtt ctttgaagtt gacggacccc aaaagatgaa gggctgcttc
      841 caaacctttg acctgggctg tcctgatgag agcatccagc ttcaaatctc gcagcagcac
      901 atcaacaaga gcttcaggca ggcagtatca ctcattgtgg ctgtggagaa gctgtggcag
      961 ctacctgtgt ctttcccgtg gaccttccag gatgaggaca tgagcacctt cttttccttc
     1021 atctttgaag aagagcccat cctctgtgac tcatgggatg atgatgataa cctgctggtg
     1081 tgtgacgttc ccattagaca actgcactac gggctccgag atgaacaaca aaaaagcctc
     1141 gtgctgtcgg acccatatga gctgaaagct ctccacctca atggacagaa tatcaaccaa
     1201 caagtgatat tctccatgag ctttgtacaa ggagaaccaa gcaacgacaa aatacctgtg
     1261 gccttgggcc tcaaaggaaa gaatctatac ctgtcctgtg taatgaaaga cggcacaccc
     1321 accctgcagc tggagagtgt ggatcccaag caatacccaa agaagaagat ggaaaaacgg
     1381 tttgtcttca acaagataga agtcaagagc aaagtggagt ttgagtctgc agagttcccc
     1441 aactggtaca tcagcacctc acaagcagag cacaagcctg tcttcctggg aaacaacagt
     1501 ggtcaggaca taattgactt caccatggaa tctgtgtctt cctaannngg gccctattct
     1561 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1621 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1681 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1741 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1801 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagtg gcggtaatac
     1861 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1921 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1981 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2041 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2101 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2161 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2221 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2281 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2341 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     2401 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2461 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2521 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2581 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2641 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2701 aacctgaggc tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac
     2761 ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg
     2821 gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa
     2881 acgacccaac accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt
     2941 ccggtattgt ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca
     3001 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     3061 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
     3121 cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
     3181 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     3241 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     3301 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     3361 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct
     3421 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt gatcatcctg
     3481 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     3541 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     3601 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     3661 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     3721 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc
     3781 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     3841 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     3901 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
     3961 tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     4021 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     4081 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     4141 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     4201 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     4261 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt
     4321 tctttccgcc tcaggactct tcctttttca ataaatcaat ctaaagtata tatgagtaaa
     4381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     5041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     5401 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5461 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5521 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5581 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5641 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5701 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5761 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     5821 aaatattaac gcttacaatt tac
//
