LOCUS       V008203                 7098 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008203
VERSION     V008203
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7098)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7098)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        638..656
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             700..723
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             733..2745
                     /gene="RIPK1"
                     /label="Receptor-interacting serine/threonine-protein
                     kinase 1"
                     /note="Receptor-interacting serine/threonine-protein kinase
                     1 from Homo sapiens. Accession#: Q13546"
     promoter        complement(2813..2831)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2857..3081
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3213..3801)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(4130..4177)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4412..5197)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5232..5589)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             complement(5652..6509)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6510..6614)
                     /label="AmpR promoter"
     rep_origin      6641..7096
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccggatc ccccgggaat caaaacatgg attacaaaga cgatgacgat
      721 aaagaattca ggatgcaacc agacatgtcc ttgaatgtca ttaagatgaa atccagtgac
      781 ttcctggaga gtgcagaact ggacagcgga ggctttggga aggtgtctct gtgtttccac
      841 agaacccagg gactcatgat catgaaaaca gtgtacaagg ggcccaactg cattgagcac
      901 aacgaggccc tcttggagga ggcgaagatg atgaacagac tgagacacag ccgggtggtg
      961 aagctcctgg gcgtcatcat agaggaaggg aagtactccc tggtgatgga gtacatggag
     1021 aagggcaacc tgatgcacgt gctgaaagcc gagatgagta ctccgctttc tgtaaaagga
     1081 aggataattt tggaaatcat tgaaggaatg tgctacttac atggaaaagg cgtgatacac
     1141 aaggacctga agcctgaaaa tatccttgtt gataatgact tccacattaa gatcgcagac
     1201 ctcggccttg cctcctttaa gatgtggagc aaactgaata atgaagagca caatgagctg
     1261 agggaagtgg acggcaccgc taagaagaat ggcggcaccc tctactacat ggcgcccgag
     1321 cacctgaatg acgtcaacgc aaagcccaca gagaagtcgg atgtgtacag ctttgctgta
     1381 gtactctggg cgatatttgc aaataaggag ccatatgaaa atgctatctg tgagcagcag
     1441 ttgataatgt gcataaaatc tgggaacagg ccagatgtgg atgacatcac tgagtactgc
     1501 ccaagagaaa ttatcagtct catgaagctc tgctgggaag cgaatccgga agctcggccg
     1561 acatttcctg gcattgaaga aaaatttagg cctttttatt taagtcaatt agaagaaagt
     1621 gtagaagagg acgtgaagag tttaaagaaa gagtattcaa acgaaaatgc agttgtgaag
     1681 agaatgcagt ctcttcaact tgattgtgtg gcagtacctt caagccggtc aaattcagcc
     1741 acagaacagc ctggttcact gcacagttcc cagggacttg ggatgggtcc tgtggaggag
     1801 tcctggtttg ctccttccct ggagcaccca caagaagaga atgagcccag cctgcagagt
     1861 aaactccaag acgaagccaa ctaccatctt tatggcagcc gcatggacag gcagacgaaa
     1921 cagcagccca gacagaatgt ggcttacaac agagaggagg aaaggagacg cagggtctcc
     1981 catgaccctt ttgcacagca aagaccttac gagaattttc agaatacaga gggaaaaggc
     2041 actgtttatt ccagtgcagc cagtcatggt aatgcagtgc accagccctc agggctcacc
     2101 agccaacctc aagtactgta tcagaacaat ggattatata gctcacatgg ctttggaaca
     2161 agaccactgg atccaggaac agcaggtccc agagtttggt acaggccaat tccaagtcat
     2221 atgcctagtc tgcataatat cccagtgcct gagaccaact atctaggaaa tacacccacc
     2281 atgccattca gctccttgcc accaacagat gaatctataa aatataccat atacaatagt
     2341 actggcattc agattggagc ctacaattat atggagattg gtgggacgag ttcatcacta
     2401 ctagacagca caaatacgaa cttcaaagaa gagccagctg ctaagtacca agctatcttt
     2461 gataatacca ctagtctgac ggataaacac ctggacccaa tcagggaaaa tctgggaaag
     2521 cactggaaaa actgtgcccg taaactgggc ttcacacagt ctcagattga tgaaattgac
     2581 catgactatg agcgagatgg actgaaagaa aaggtttacc agatgctcca aaagtgggtg
     2641 atgagggaag gcataaaggg agccacggtg gggaagctgg cccaggcgct ccaccagtgt
     2701 tccaggatcg accttctgag cagcttgatt tacgtcagcc agaacctgaa ttctgcagat
     2761 atccatcaca ctggcggccg ctcgagtaga tgactagtct agagggccct attctatagt
     2821 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     2881 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     2941 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3001 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3061 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagtggcggt aatacggtta
     3121 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3181 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3241 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3301 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3361 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3421 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3481 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3541 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3601 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     3661 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3721 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3781 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3841 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3901 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaacct
     3961 gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa
     4021 cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca atggggtctc
     4081 ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac
     4141 ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt
     4201 attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg
     4261 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     4321 ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg
     4381 tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     4441 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     4501 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     4561 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     4621 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct
     4681 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttgatca tcctgatcga
     4741 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     4801 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     4861 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     4921 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     4981 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     5041 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     5101 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     5161 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     5221 cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
     5281 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     5341 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     5401 actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     5461 ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     5521 gctggggagc ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt
     5581 ccgcctcagg actcttcctt tttcaataaa tcaatctaaa gtatatatga gtaaacttgg
     5641 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5701 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5761 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5821 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5881 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5941 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6001 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6061 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6121 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6181 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6241 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6301 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6361 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6421 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6481 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6541 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6601 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     6661 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6721 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6781 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6841 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6901 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6961 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7021 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     7081 ttaacgctta caatttac
//
