LOCUS       40924_13220        4759 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCR2.1-gentR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     A System for Site-Specific Genetic Manipulation of the Relapsing 
            Fever Spirochete Borrelia hermsii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky 
            Mountain Laboratories, National Institute of Allergy and Infectious 
            Diseases, National Institutes of Health, 903 S. 4th Street, 
            Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 4759)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4759)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky Mountain 
            Laboratories, National Institute of Allergy and Infectious Diseases,
            National Institutes of Health, 903 S. 4th Street, Hamilton, MT 
            59840, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(323..853)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     regulatory      complement(854..1139)
                     /label=flaB promoter derived from Borrelia hermsii
                     /note="flaB promoter derived from Borrelia hermsii"
                     /regulatory_class="promoter"
     promoter        complement(1216..1234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1241..1257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1399..1779
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             2147..2938
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             2959..3816
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3990..4578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt
      361 cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt
      421 gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg
      481 cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct
      541 cactacgcgg ctgctcaaac ctgggcagaa cgtaagccgc gagagcgcca acaaccgctt
      601 cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat
      661 cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat
      721 caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc
      781 ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc
      841 tgctgcgtaa catatgtcat ttcctccgtg ataaattaat ataattttaa tatttagaca
      901 aatccatttg tctttttaaa aggatatcac aaaaacaaga aaaaaataaa tacaatatta
      961 atttaaatta aatttgttac ctgttttgtc gctgtaaatt caaagattta aggatttcct
     1021 cttgttcctt catccgcttt acagtgaaat ccaaagtatt aagttctcct tcaaccttat
     1081 tctccctttc ctcatacttc tttctataat cttcaaccac atttttctga ttaggaattg
     1141 ccggcaaggg cgaattctgc agatatccat cacactggcg gccgctcgag catgcatcta
     1201 gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     1261 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     1321 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     1381 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     1441 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     1501 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     1561 ctttagggtt ccgatttaga gctttacggc acctcgaccg caaaaaactt gatttgggtg
     1621 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     1681 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatcgcgg
     1741 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     1801 tgatttaaca aattcagggc gcaagggctg ctaaaggaac cggaacacgt agaaagccag
     1861 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
     1921 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
     1981 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     2041 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     2101 caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     2161 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     2221 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     2281 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     2341 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     2401 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     2461 tcgccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     2521 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     2581 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     2641 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     2701 cgtgatccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     2761 attcaacgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggatac
     2821 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     2881 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     2941 aattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3001 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3061 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3121 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3181 tgtcatacac tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgggcgcgg
     3241 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3301 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3361 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3421 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3481 gagagtgaca ccacgatgcc tgtagcaatg ccaacaacgt tgcgcaaact attaactggc
     3541 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3601 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3661 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3721 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3781 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3841 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3901 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3961 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4021 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4081 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4141 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4201 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4261 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4321 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4381 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4441 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4501 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4561 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4621 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4681 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4741 gtgagcgagg aagcggaag
//
