LOCUS       40924_13170        8791 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPP5702, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh HS, Collmer A.
  TITLE     Identification of harpins in Pseudomonas syringae pv. tomato DC3000,
            which are functionally similar to HrpK1 in promoting translocation 
            of type III secretion system effectors
  JOURNAL   J. Bacteriol. 189 (22), 8059-8072 (2007)
  PUBMED    17873033
REFERENCE   2  (bases 1 to 8791)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh H-S., Collmer A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2007) Plant Pathology, Cornell University, 334 
            Plant Science, Ithaca, NY 14853, USA
REFERENCE   3  (bases 1 to 8791)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2007"; volume: "189"; issue: "22"; pages: 
            "8059-8072"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2007) Plant Pathology, Cornell University, 334 Plant 
            Science, Ithaca, NY 14853, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(46..397)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             411..1241
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     1405..1421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1425..1548
                     /label=omega
                     /note="omega"
                     /regulatory_class="terminator"
     CDS             complement(2036..2827)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(3341..3464)
                     /label=omega
                     /note="omega"
                     /regulatory_class="terminator"
     regulatory      3465..3497
                     /label=hrp box
                     /note="hrp box"
                     /regulatory_class="promoter"
     protein_bind    complement(4040..4064)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5305..5321)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5329..5345)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5353..5383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5398..5419)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5707..6295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7120..8307)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(8355..8383)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     protein_bind    8630..8651
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8666..8696
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8704..8720
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8728..8744
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8762..8780
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 cctctcaggc gccgctggtg ccgctggttg gacgccaagg gtgaatccgc ctcgataccc
       61 tgattactcg cttcctgcgc cctctcaggc ggcgataggg gactggtaaa acggggattg
      121 cccagacgcc tcccccgccc cttcaggggc acaaatgcgg ccccaacggg gccacgtagt
      181 ggtgcgtttt ttgcgtttcc acccttttct tccttttccc ttttaaacct tttaggacgt
      241 ctacaggcca cgtaatccgt ggcctgtaga gtttaaaaag ggacggattt gttgccatta
      301 agggacggat ttgttgttaa gaagggacgg atttgttgtt gtaaagggac ggatttgttg
      361 tattgtggga cgcagataca gtgtcccctt atacacaagg aatgtcgaac gtggcctcac
      421 ccccaatggt ttacaaaagc aatgccctgg tcgaggccgc gtatcgcctc agtgttcagg
      481 aacagcggat cgttctggcc tgtattagcc aggtgaagag gagcgagcct gtcaccgatg
      541 aagtgatgta ttcagtgacg gcggaggaca tagcgacgat ggcgggtgtc cctatcgaat
      601 cttcctacaa ccagctcaaa gaagcggccc tgcgcctgaa acggcgggaa gtccggttaa
      661 cccaagagcc caatggcaag gggaaaagac cgagtgtgat gattaccggc tgggtgcaaa
      721 caatcatcta ccgggagggt gagggccgtg tagaactcag gttcaccaaa gacatgctgc
      781 cgtacctgac ggaactcacc aaacagttca ccaaatacgc cttggctgac gtggccaaga
      841 tggacagcac ccacgcgatc aggctttacg agctgctcat gcaatgggac agcatcggcc
      901 agcgcgaaat agaaattgac cagctgcgaa agtggtttca actggaaggc cggtatccct
      961 cgatcaagga cttcaagttg cgagtgcttg atccagccgt gacgcagatc aacgagcaca
     1021 gcccgctaca ggtggagtgg gcgcagcgaa agaccgggcg caaggtcaca catctgttgt
     1081 tcagttttgg accgaagaag cccgccaagg cggtgggtaa ggccccagcg aagcgcaagg
     1141 ccgggaagat ttcagatgct gagatcgcga aacaggctcg ccctggtgag acatgggaag
     1201 cggcccgcgc tcgactaacc cagatgccgc tggatctggc ctagaggccg tggccaccac
     1261 ggcccggcct gcctttcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     1321 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     1381 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt tatgcttgta
     1441 aaccgttttg tgaaaaaatt tttaaaataa aaaaggggac ctctagggtc cccaattaat
     1501 tagtaatata atctattaaa ggtcattcaa aaggtcatcc accggatcaa ttcccctgct
     1561 cgcgcaggct gggtgccaag ctctcgggta acatcaaggc ccgatccttg gagcccttgc
     1621 cctcccgcac gatgatcgtg ccgtgatcga aatccagatc cttgacccgc agttgcaaac
     1681 cctcactgat ccgccaaagc ggccatcgtg cctccccact cctgcagttc gggggcatgg
     1741 atgcgcggat agccgctgct ggtttcctgg atgccgacgg atttgcactg ccggtagaac
     1801 tccgcgaggt cgtccagcct caggcagcag ctgaaccaac tcgcgagggg atcgagcccg
     1861 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
     1921 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
     1981 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga
     2041 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     2101 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     2161 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     2221 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
     2281 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     2341 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     2401 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     2461 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     2521 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     2581 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     2641 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     2701 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     2761 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     2821 caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc
     2881 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
     2941 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
     3001 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
     3061 tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt
     3121 tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaca
     3181 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     3241 taatgtctaa caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt
     3301 agatgcacta agcacataat tgctcacagc caaactatca gatccggtgg atgacctttt
     3361 gaatgacctt taatagatta tattactaat taattgggga ccctagaggt cccctttttt
     3421 attttaaaaa ttttttcaca aaacggttta caagcataaa gcttggaacc gatccgctcc
     3481 ctatgaccac tcaagtggcg tcataaatgc tgtactaaag agggtatacg aatgggaaat
     3541 atatgtgtcg gcggatccag gatggcccat caggtgaact ccccagaccg agttagtaac
     3601 aactcgggtg acgaagataa cgtaacgtcc agtcaactgc tgagcgtcag acatcaactt
     3661 gcggagtctg ctggtgtacc aagagatcag catgaatttg ttagtaacca agcacctcaa
     3721 agcctgagaa atcgctacaa caatctttac tcacatacgc aaagaacact ggatatggcg
     3781 gacatgcagc ataggtacat gacgggagcg tcaggaatca atccgggaat gctgccacat
     3841 gagaatgtgg acgatatgcg tagcgctata actgattgga gtgacatgcg cgaagctctg
     3901 cagtacgcaa tgggtatcca tgccgacatc ccaccgtctc cagagcgatt tgttgcgact
     3961 atgaacccga acggatcaat tcgaatgtca acactttctc ctagcccgta ccgtaactgg
     4021 caaaagggtg ggcgcgccga cccagctttc ttgtacaaag tggttgatgg gcagcaatcg
     4081 catcaggctg gttacgcaaa cgccgccgac cgggagtctg gcatccccgc agccgtactc
     4141 gatggcatca aggccgtggc gaaggaaaaa aacgccacat tgatgttccg cctggtcaac
     4201 ccccattcca ccagcctgat tgccgaaggg gtggccacca aaggattggg cgtgcacgcc
     4261 aagtcgtccg attgggggtt gcaggcgggc tacattcccg tcaacccgaa tctttccaaa
     4321 ctgttcggcc gtgcgcccga ggtgatcgcg cgggccgaca acgacgtcaa cagcagcctg
     4381 gcgcatggcc ataccgcggt cgacctgacg ctgtcgaaag agcggcttga ctatctgcgg
     4441 caagcgggcc tggtcaccgg catggccgat ggcgtggtcg cgagcaacca cgcaggctac
     4501 gagcagttcg agtttcgcgt gaaggaaacc tcggacgggc gctatgccgt gcagtatcgc
     4561 cgcaagggcg gcgacgattt cgaggcggtc aaggtgatcg gcaatgccgc cggtattcca
     4621 ctgacggcgg atatcgacat gttcgccatt atgccgcatc tgtccaactt ccgcgactcg
     4681 gcgcgcagtt cggtgaccag cggcgattcg gtgaccgatt acctggcgcg cacgcggcgg
     4741 gccgccagcg aggccacggg cggcctggat cgcgaacgca tcgacttgtt gtggaaaatc
     4801 gctcgcgccg gcgcccgttc cgcagtgggc accgaggcgc gtcgccagtt ccgctacgac
     4861 ggcgacatga atatcggcgt gatcaccgat ttcgagctgg aagtgcgcaa tgcgctgaac
     4921 aggcgggcgc acgccgtcgg cgcgcaggac gtggtccagc atggcactga gcagaacaat
     4981 cctttcccgg aggcagatga gaagattttc gtcgtatcgg ccaccggtga aagccagatg
     5041 ctcacgcgcg ggcaactgaa ggaatacatt ggccagcagc gcggcgaggg ctatgtcttc
     5101 tacgagaacc gtgcatacgg cgtggcgggg aaaagcctgt tcgacgatgg gctgggagcc
     5161 gcgcccggcg tgccgagcgg acgttcgaag ttctcgccgg atgtactgga aacggtgccg
     5221 gcgtcacccg gattgcggcg gccgtcgctg ggcgcagtgg aacgccagga ttccggctat
     5281 gacagctaag aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5341 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5401 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5461 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5521 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5581 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5641 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5701 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5761 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5821 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5881 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5941 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6001 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6061 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6121 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6181 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6241 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6301 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6361 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6421 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6481 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6541 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6601 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6661 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6721 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6781 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6841 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6901 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6961 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagtc
     7021 aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag
     7081 tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca
     7141 tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc
     7201 caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt
     7261 gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc
     7321 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag
     7381 aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc
     7441 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
     7501 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
     7561 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
     7621 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
     7681 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
     7741 gcgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
     7801 gagcaccgcc gccgcaagga atggtgcatg taaggagatg gcgcccaaca gtcccccggc
     7861 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     7921 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     7981 ggtgatgccg gccacgatgc gtccggcgta gagaatccac aggacgggtg tggtcgccat
     8041 gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa
     8101 gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc
     8161 tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc
     8221 cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat
     8281 gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg
     8341 tgataaacta ccgcattaaa gctaatcgat gataagctgt caaacatgag aattaattca
     8401 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8461 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8521 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8581 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8641 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8701 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8761 tatttaggtg acactataga atactcaagc t
//
