LOCUS       40924_13120        8766 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPP39, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Leveau JH, Lindow SE.
  TITLE     Predictive and interpretive simulation of green fluorescent protein 
            expression in reporter bacteria
  JOURNAL   J. Bacteriol. 183 (23), 6752-6762 (2001)
  PUBMED    11698362
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Remus-Emsermann MN, Leveau JH.
  TITLE     Linking environmental heterogeneity and reproductive success at 
            single-cell resolution
  JOURNAL   ISME J 4 (2), 215-222 (2010)
  PUBMED    19865185
REFERENCE   3  (bases 1 to 8766)
  AUTHORS   Remus-Emsermann MNP., Leveau JHJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2010) Plant Pathology, UC-Davis, One Shields 
            Avenue, Davis, CA 95616-8751, USA
REFERENCE   4  (bases 1 to 8766)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8766)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2001"; volume: "183"; issue: "23"; pages: 
            "6752-6762"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "ISME J"; 
            date: "2010"; volume: "4"; issue: "2"; pages: "215-222"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-JUL-2010) Plant Pathology, UC-Davis, One Shields Avenue, Davis, 
            CA 95616-8751, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    53..74
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        89..119
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    127..143
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    183..290
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1455..2102)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             2208..3404
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(3465..4544)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4545..4622)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             complement(5927..7072)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7347..7715)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(7748..7857)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      8002..8711
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
       61 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      121 gtggaattgt gagcggataa caatttcaca caggaaacag ctatcgacat gattacgaat
      181 tcgagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat
      241 tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc gatgcatgca
      301 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgagcttc gggctgaaat
      481 tgcaggacaa ctggtgagtc tggatctgaa gttcattgtt gtcatggatg atctggaccg
      541 actggagcca tcccaggtgg cggaagtgtt caggcttgtg cgtgcagtag ccgatctgcc
      601 ccgctttacc catattctct gttatgacag gcagattatc actcatgccg ttgaacatgc
      661 gctgaatatc gaagatggca gccgttatct ccagaaaatc attcagctta gttttaaatt
      721 accccgacct gaagcctttg atttacgtaa tgaatttcgc cagcgggctg aggctctata
      781 tcagcaaatt aataatcaac cgccagactc tggaatggta agggatctca tcgcggtgac
      841 tgatacctat ggtgccgcac tttcgacgcc acgggaaatc catcaggcca ttaattccct
      901 gatttttctt tatccgggga tgcgggattt tgtttatttc cctgatttgt gcctgcttca
      961 gcttatacgg gtgacaaacc cggctctgta tgactggaca gagcattacc tgacagaacg
     1021 gtccgtgatt gaaaccggtc agggtatgct ttctgacgga gagaaagcag acttccggga
     1081 ggggcttatc agatgtatga agacgttcag ggcatcaaat gcagactcgt ttctgacact
     1141 tgcagactgg atctatctca tctgcgcaag gcagaacgtg aagacggccg ccctggacct
     1201 cgcccgcgag cgccaggcgc acgaggccgg cgcgcggacc cgcgccacgg cccacgagcg
     1261 gacgccgcag caggagcgcc agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg
     1321 gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg
     1381 gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt gcgctccggc
     1441 agcggtcctg atcaatcgtc accctttctc ggtccttcaa cgttcctgac aacgagcctc
     1501 cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggaccg
     1561 gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg
     1621 gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca
     1681 gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg
     1741 cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg
     1801 gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc
     1861 ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc
     1921 gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa
     1981 agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg
     2041 ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc
     2101 atgcttgaca ctttatcact gataaacata atatgtccac caacttatca gtgataaaga
     2161 atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca
     2221 tacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta
     2281 tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact
     2341 atggcattct gctggcgctg tatgcgttgg tgcaatttgc ctgcgcacct gtgctgggcg
     2401 cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt ctcgctggcc ggcgccactg
     2461 tcgactacgc catcatggcg acagcgcctt tcctttgggt tctctatatc gggcggatcg
     2521 tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc gatatcactg
     2581 atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg
     2641 tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct ccgttcttcg
     2701 ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg ccggagtcgc
     2761 acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct tcgttccggt
     2821 gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc atgcaacttg
     2881 tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt cactgggacg
     2941 cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc gcccaggcaa
     3001 tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga
     3061 ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg atggcgttcc
     3121 cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt
     3181 ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg gcgctcacca
     3241 gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct tctataacaa
     3301 cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc ctgccggcgc
     3361 tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg tggaaacgat
     3421 aagcttgcaa ttcgcgctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     3481 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3541 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     3601 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     3661 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     3721 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     3781 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     3841 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     3901 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     3961 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     4021 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     4081 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     4141 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     4201 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     4261 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     4321 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     4381 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     4441 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     4501 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     4561 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca ttcgatggtg
     4621 tcaacgtaaa tgcatgccgc ttcgccttcg cgcgcgaatt gcaagctcct atgccatgcg
     4681 ggtcaaggcg acttccggca agctatacgc gccctaggag tgcggttgga acgttggccc
     4741 agccagatac tcccgatcac gagcaggacg ccgatgattt gaagcgcact cagcgtctga
     4801 tccaagaaca accatcctag caacacggcg gtccccgggc tgagaaagcc cagtaaggaa
     4861 acaactgtag gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt taaacccgct
     4921 ccgatcaggc cgagccacgc caggccgaga acattggttc ctgtaggcat cgggattggc
     4981 ggatcaaaca ctaaagctac tggaacgagc agaagtcctc cggccgccag ttgccaggcg
     5041 gtaaaggtga gcagaggcac gggaggttgc cacttgcggg tcagcacggt tccgaacgcc
     5101 atggaaaccg cccccgccag gcccgctgcg acgccgacag gatctagcgc tgcgtttggt
     5161 gtcaacacca acagcgccac gcccgcagtt ccgcaaatag cccccaggac cgccatcaat
     5221 cgtatcgggc tacctagcag agcggcagag atgaacacga ccatcagcgg ctgcacagcg
     5281 cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg aaataaacaa caagctccag
     5341 aatagcgaaa tattaagtgc gccgaggatg aagatgcgca tccaccagat tcccgttgga
     5401 atctgtcgga cgatcatcac gagcaataaa cccgccggca acgcccgcag cagcataccg
     5461 gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg cgccttgtga
     5521 gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc caatgatctc gccgtcgatg
     5581 taggcgccga atgccacggc atctcgcaac cgttcagcga acgcctccat gggctttttc
     5641 tcctcgtgct cgtaaacgga cccgaacatc tctggagctt tcttcagggc cgacaatcgg
     5701 atctcgcgga aatcctgcac gtcggccgct ccaagccgtc gaatctgagc cttaatcaca
     5761 attgtcaatt ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc
     5821 gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag
     5881 cgctggctgc tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca
     5941 ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg
     6001 cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc
     6061 ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc
     6121 gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc
     6181 cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc
     6241 cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt
     6301 cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt
     6361 aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga
     6421 tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt
     6481 catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga
     6541 aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg
     6601 cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga
     6661 acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct
     6721 tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca
     6781 tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac
     6841 gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc
     6901 tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac tggaaggttt
     6961 cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc
     7021 ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc
     7081 cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga tttgacccgc
     7141 ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc ccgtagaccg
     7201 tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat accagctccg
     7261 cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc
     7321 ggccgtttta gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg
     7381 accttgccaa gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca
     7441 tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg
     7501 cggcccaggt cgccattgat gcgggccagc tcgcggacgt gctcatagtc cacgacgccc
     7561 gtgattttgt agccctggcc gacggccagc aggtaggccg acaggctcat gccggccgcc
     7621 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg
     7681 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
     7741 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
     7801 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
     7861 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
     7921 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
     7981 gcagcggaaa agatccgtcg accctttccg acgctcaccg ggctggttgc cctcgccgct
     8041 gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg
     8101 agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga
     8161 tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag
     8221 gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac
     8281 gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg
     8341 cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt
     8401 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca
     8461 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc
     8521 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg
     8581 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc
     8641 taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc
     8701 aaaaatggca gccaagccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8761 aatgca
//
