LOCUS       V008226                 6854 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008226
VERSION     V008226
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Geminivirus-based vectors for gene silencing in Arabidopsis
  JOURNAL   Plant J. 30 (1), 107-114 (2002)
   PUBMED   11967097
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Robertson D, Peele CG.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2003) Botany, North Carolina State University, Box
            7612, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 6854)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6854)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
            date: "2002"; volume: "30"; issue: "1"; pages: "107-114"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-APR-2003) Botany, North Carolina State University, Box 7612,
            Raleigh, NC 27695, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..858)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(859..963)
                     /label="AmpR promoter"
     rep_origin      complement(989..1444)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1586..1602
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1612..1630
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1656..1672
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2492..3259
                     /note="Nuclear shuttle protein from Cabbage leaf curl virus
                     (isolate Jamaica). Accession#: Q96706"
                     /label="Nuclear shuttle protein"
     primer_bind     complement(5599..5615)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(5652..5670)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5691..5707)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5715..5731)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5739..5769)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5784..5805)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6093..6681)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
       61 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
      481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      961 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     1021 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     1081 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     1141 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     1201 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     1261 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     1321 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     1381 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     1441 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     1501 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     1561 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     1621 tcactatagg gcgaattggg taccgggccc cccctcgagg tcgacggtat cgataagctt
     1681 gatatcgaat tcatttatag aaatccgacc gcgcagcggc aggggcttca ataatatctg
     1741 ggtttagtaa aaactgtgtt gaagaacaga aaaatgaaac gacctgtttc gaaaggagga
     1801 agagagaaaa tctgggtttc taaacagaaa gggaataacg atagttctaa cgaaggatga
     1861 gtatgttagg ttattcagat aacatgagta taataatcta agactaagtc tgattatata
     1921 gagagcaaat ttgaagaaat taaaaaagct tcgaatgtta tgtttagtgg catattcgta
     1981 aatatgttca tggacaccag gagagctctc gtctaaaacc tattgtttct ggtgtcttgg
     2041 tgtcctataa atactaaaag cctctgggga ctccatagga cacgtgtaca catctcagcg
     2101 gccatccgta ataatattac cggatggccg caaatttttg gagtcctggt gctggggacc
     2161 atctcccgct cccctttggc gcactctctc tcatctctcg acgtggcgcg tcgaggagcg
     2221 ttggctaacg cttatctcgg tgttaaacct ttaatttgaa gttcgaaata attgggcgta
     2281 tatgtctatc ttgaccggtc tttgtaacga caaagcttct gacacattgt acaatatatt
     2341 gaacgtggcc caattatatt tcctctacgg agttaggcta tcgcttattt gtatttgcct
     2401 tgtctatata atggacgata gtttaatatt ttaaatcgtc tttgacaata acattaaaat
     2461 aacaaatcat tttgtagagg tgctgagaat aatgtatcct acaaagttta ggcgtggggt
     2521 atcttactct caaagacgat ttgtttcacg taatcaatcg tctaagcgtg gaacttttgt
     2581 tagacgcact gatgggaaac gtcgtaaagg cccatcaagt aaagcccatg atgagcctaa
     2641 aatgaagttg caacgcatac atgaaaatca atatgggcct gaatttgtca tgacccataa
     2701 ctcagccctt tcaacgttta ttaatttccc tgtacttggt aagattgaac ctaaccgaag
     2761 caggtcgtat attaagttga accggttatc atttaaggga accgttaaga ttgagcgtgt
     2821 acatgctgat gtgaacatgg acggagtaat ttcgaagata gagggtgtgt tctctcttgt
     2881 tattgttgtt gatcgcaaac cacatttaag ctccactgga ggtttgcata catttgatga
     2941 aatatttggt gcaagaatcc atagccatgg gaacttagct attacacccg gtttgaaaga
     3001 tcgttattac gtcctccatg ttttgaaacg cgtattgtct gtggagaaag acactttgat
     3061 ggtggatctt gaaggatcta ccacgatatc taataggcgt tataactgtt gggcctcatt
     3121 taacgatctt gaacatgact tatgtaacgg tgtttatgcg aatataagca aaaacgccat
     3181 tttggtatat tattgttgga tgtccgatgc tatgtctaag gcatcgacct ttgtatctta
     3241 cgatcttgat tatttaggtt aaccatgaat aaaatggcgt taagattgaa tatatgcatt
     3301 aataataaaa cacgcaattt tattgcaatg actttggttg tggaggatta caattattgt
     3361 taatacattc ctgaaccgtc gtcctaacta gctcgcttaa ttgggccact gacatcgtta
     3421 tggttgattg ggccctctgt aacccagctt gtgatgctga atccccgggg tctaatgcgc
     3481 tagttcctag caggttgagt tctctatatg gatgtagcgc gttttccact tctgattctg
     3541 tgtgtgggtt gggaaaccca attgtgctcc ttgaagccca tgaatcacct ggttgtaatt
     3601 caattgggcc tgttagtcca attcttgaca atgatttgga cctcaaggtc ttcctctccc
     3661 atcttccgta gtccacatgt gagaaatcaa catctctatg tgaaaattgt tttgaatgaa
     3721 ttttcactgt tggtgcccgg aaggggatat ccactgaatg tttagctgtg gacaatttca
     3781 atttcccctt aaacttggca aaatgtgttc gttgatgcac atttgtgtcg ctaactctgt
     3841 aatagagttt ccacggaatg gggtctttta gagagaagaa tgaagatgaa aaatagtgga
     3901 gatctatgtt gcatcttaag ggaaatgtcc aagacgcttg taaggattcg tcgtcagtca
     3961 tcctcttgtc atggatctcc actatcacag atccagttgc gttaattggt acttgctgtc
     4021 tatattcgat gacgcaatgg tcgattttca tacagctacg attaagcctt gctgtaaatt
     4081 gcgctgcagt tgaagggaat tgaagtagta tctcagtaag atcatgcgac agctgatatt
     4141 cgtctctatg agactctatg taattaaacg catttggagc attcgctaac tgagaattca
     4201 tttatagaaa tccgaccgcg cagcggcagg ggcttcaata atatctgggt ttagtaaaaa
     4261 ctgtgttgaa gaacagaaaa atgaaacgac ctgtttcgaa aggaggaaga gagaaaatct
     4321 gggtttctaa acagaaaggg aataacgata gttctaacga aggatgagta tgttaggtta
     4381 ttcagataac atgagtataa taatctaaga ctaagtctga ttatatagag agcaaatttg
     4441 aagaaattaa aaaagcttcg aatgttatgt ttagtggcat attcgtaaat atgttcatgg
     4501 acaccaggag agctctcgtc taaaacctat tgtttctggt gtcttggtgt cctataaata
     4561 ctaaaagcct ctggggactc cataggacac gtgtacacat ctcagcggcc atccgtaata
     4621 atattaccgg atggccgcaa atttttggag tcctggtgct ggggaccatc tcccgctccc
     4681 ctttggcgca ctctctctca tctctcgacg tggcgcgtcg aggagcgttg gctaacgctt
     4741 atctcggtgt taaaccttta atttgaagtt cgaaataatt gggcgtatat gtctatcttg
     4801 accggtcttt gtaacgacaa agcttctgac acattgtaca atatattgaa cgtggcccaa
     4861 ttatatttcc tctacggagt taggctatcg cttatttgta tttgccttgt ctatataatg
     4921 gacgatagtt taatatttta aatcgtcttt gacaataaca ttaaaataac aaatcatttt
     4981 gtagaggtgc tgagaataat gtatcctaca aagtttaggc gtggggtatc ttactctcaa
     5041 agacgatttg tttcacgtaa tcaatcgtct aagcgtggaa cttttgttag acgcactgat
     5101 gggaaacgtc gtaaaggccc atcaagtaaa gcccatgatg agcctaaaat gaagttgcaa
     5161 cgcatacatg aaaatcaata tgggcctgaa tttgtcatga cccataactc agccctttca
     5221 acgtttatta atttccctgt acttggtaag attgaaccta accgaagcag gtcgtatatt
     5281 aagttgaacc ggttatcatt taagggaacc gttaagattg agcgtgtaca tgctgatgtg
     5341 aacatggacg gagtaatttc gaagatagag ggtgtgttct ctcttgttat tgttgttgat
     5401 cgcaaaccac atttaagctc cactggaggt ttgcatacat ttgatgaaat atttggtgca
     5461 agaatccata gccatgggaa cttagctatt acacccggtt tgaaagatcg ttattacgtc
     5521 ctccatgttt tgaaacgcgt attgtctgtg gagaaagaca ctttgatggt ggatcttgaa
     5581 ggatctacca cgatggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
     5641 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     5701 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5761 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5821 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5881 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5941 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6001 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6061 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6481 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     6541 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6601 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6661 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6721 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6781 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6841 tggtctgaca gtta
//
