LOCUS       40924_13105        7419 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC82, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7419)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7419)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    124..394
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             795..1508
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     rep_origin      complement(1600..2188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2362..3219)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3220..3324)
                     /label=AmpR promoter
     regulatory      3395..4129
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             4130..4747
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             4769..5110
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      5128..5434
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     CDS             5781..7187
                     /codon_start=1
                     /product="Arg4"
                     /label=Arg4
                     /protein_id="AVR52663.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
ORIGIN
        1 cagtcagtca gtcaggcttg gacagcatca gcagtagcgt attgttcgaa tgaaccatcg
       61 tgagtgtaac cagacaagtc agcttcacca cagtttggtt cagcaccttg tcgagtttac
      121 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      181 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      241 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      301 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      361 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagc tccaaacttc
      421 ttttttattt ttcgcaatat tgtcgataaa ttaaatataa actactcgat tgagtaatag
      481 attcaataac tatgggaatt actaacaatg atttaaggaa aaagcttcac ttaaaaataa
      541 agccctttca attaaagtgt tgtccatgtt tgtcgtttct gatgggcttt tctgaaatat
      601 aaatatagat tcaaatctct tgtattcaag ttttagattt ttttttattt cttttatatc
      661 attatcaaat caaatcaaca aactctttca acttctcaga aagaaatcaa tatcaactac
      721 aataaaacca actacgtcta ctactacgtt aactcctaca cacatacaaa tataaatagt
      781 tattcatgtc cacaatctct aaaggtgaag aattattcac tggtgttgtc ccaattttgg
      841 ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg
      901 atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc
      961 catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg agatacccag
     1021 atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa
     1081 gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag
     1141 gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca
     1201 ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca
     1261 aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg
     1321 ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac
     1381 cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag
     1441 accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat
     1501 tgtacaagtc taagaactcg aggataacgc aggaaagaac atgtgagcaa aaggccagca
     1561 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1621 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1681 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1741 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1801 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1861 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1921 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1981 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2041 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2101 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2161 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2221 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2281 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2341 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2401 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2461 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2521 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2581 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2641 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2701 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2761 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2821 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2881 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2941 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3001 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3061 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3121 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3181 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3241 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3301 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     3361 accgcggagg cctgggcccc cgggtaccga gctcgccagc gagaaagagg agtattggca
     3421 ttgttgggta gtagatgaat gagtaatctt ctagccaaaa caaaataaac cacaagaaca
     3481 gtaggcaaaa ctgagtactt gatagcgctt gtagtctttg tatttaaaaa aacctaattg
     3541 tccaaaaatt gaaaatcatg agttgcaaat ttgttgaaat caccaggtaa atagatcatt
     3601 tagctatatc gccttatcat tcttcgtaca agaattatac acaaatacca ccaccacacc
     3661 acccagaaat tttacacgtg ttttctaaat tattactttc cgtggccaat caaattgaaa
     3721 tttttcttgt tcgaaacggc agtgcctcaa acattatagt agcaagtatt gcctaaagag
     3781 tttccgtctc gtgccccccc cctatcccct tttgtaattt gccaaaatag ctcaaaaagg
     3841 ataatcccgc actttttttt tccgagttct cgtcatgcaa gcaagcttat tcagaatttt
     3901 cagaggtgcg agtctcacca ctaccactgc agctgcatct tttacagcaa cagcaacagc
     3961 aggtgccacc acggcaaaga cattgtctgg atccactgtg ctccgaaaac tgtataaaag
     4021 gacctattca tccctggtct tatcttctcc agaattattt ttttttcatc agtttaacaa
     4081 caacaaacgt tattgtcata caacaacaac aacaaataca aaaacaatta tgtcaagatt
     4141 agataaatca aaagttatta attcagcttt agaattatta aatggtgttg gtattgaagg
     4201 tttaactact agaaaattag ctcaaaaatt aggtgttgaa caaccaactt tatattggca
     4261 tgttaaaaat aaaagagctt tattagatgc tttaccaatt gaaatgttag atagacatca
     4321 tactcattca tgtccattag aaggtgaatc atggcaagat tttttaagaa ataatgctaa
     4381 atcatataga tgtgctttat tatcacatag agatggtgct aaagttcatt taggtactag
     4441 accaactgaa aaacaatatg aaactttaga aaatcaatta gcttttttat gtcaacaagg
     4501 tttttcatta gaaaatgctt tatatgcttt atcagctgtt ggtcatttta ctttaggttg
     4561 tgttttagaa gaacaagaac atcaagttgc taaagaagaa agagaaactc caactactga
     4621 ttcaatgcca ccattattaa aacaagctat tgaattattt gatagacaag gtgctgaacc
     4681 agctttttta tttggtttag aattaattat ttgtggttta gaaaaacaat taaaatgtga
     4741 atcaggtggt tcgactttgt ttggtggcgc caattttaat caaagtggga atattgctga
     4801 tagctcattg tccttcactt tcactaacag tagcaacggt ccgaacctca taacaactca
     4861 aacaaattct caagcgcttt cacaaccaat tgcctcctct aacgttcatg ataacttcat
     4921 gaataatgaa atcacggcta gtaaaattga tgatggtaat aattcaaaac cattgtcacc
     4981 tggttggacg gaccaaactg cgtataacgc gtttggaatc actacaggga tgtttaatac
     5041 cactacaatg gatgatgtat ataactatct attcgatgat gaagataccc caccaaaccc
     5101 aaaaaaagag taaaccgtgc atcgctacca ttgttcacca caaatgtttc taagagtgaa
     5161 attctggaaa tctggaaatc tggttttgta ttcttgttat tcttcttttt gttattacat
     5221 atataacttg ttactttttt aaaaaaatct ttgtttattt tataaatata taaaactaaa
     5281 tttaagaaaa agagaaaaat gttttatttg agagattgaa attttacttg aatttagctt
     5341 agcttttata aagtattatt atgtaaaaaa acaaaacaaa tatacattaa aaagttaaga
     5401 ctataaaata gccacccaag gcatttctat atctacagtc tgccagactc aatttatgac
     5461 atattgaccg acataattca gtcatttcta atattcaaat aacaagacac cttgtataaa
     5521 tgaatccaca atcgtatatg aacaaccaaa ttttatgact aactttgggt cgtgtacttt
     5581 tgacgattga gtcattttta ccaattaggt aattgaattt tgttaatttg tcattaaccg
     5641 cgctggccat cccaacaata acaacaatca atttaaaaac ttgacaaggc aaaaaaaaaa
     5701 aaattttatc tttgaaaaat ttttcttcct tttcttcttc tggtctttac ataacagcta
     5761 tcaagaataa tcaattaata atgtcacaac aacaagataa acaacctagt gaaaataaat
     5821 tatggggtgg tcgtttcacc ggtgccactg atccattgat ggatttatat aatgcttcat
     5881 taccttatga taaagttatg tatgatgctg atttaactgg tactaaagta tacacacaag
     5941 gattaaacaa attaggatta attaccactg aagaattgca tttgattcat caaggattag
     6001 aacaaatcag acaagaatgg catgataata aattcattat taaggcaggt gatgaggata
     6061 ttcatactgc taatgaacgt cgattaggag aaatcattgg gaaaaacatt tctggtaaag
     6121 ttcacactgg tagatcaaga aatgatcaag ttgctaccga tatgagaatt ttcgttcgtg
     6181 aatcattact taatttaagt aaaattttac atcaattcat cacggcaatt cttgaacggg
     6241 cacataaaga aatcgatgta ttaatgcctg gttatactca tttacaaaaa gctcaaccta
     6301 ttagatgggc acattggtta agttcatatg caacttattt caccgaagat tataaacgtt
     6361 tacaagaaat tataactcgt gttaaccaat ctccattagg atctggtgct ttagcaggtc
     6421 atccttatgg tattgatcgt gaatttttag ctaaaggatt aggatttgat ggtgtcattg
     6481 ggaattcttt aactgcagtt tcagatcgtg attttgttgt tgaaagttta ttttggagta
     6541 cattatttat gaatcatatt tctcgattct cagaagatct aattatttat tctagtggtg
     6601 aatttgggtt cataaaatta gctgatgctt attctactgg ttcttcattg atgccacaaa
     6661 agaaaaaccc tgactcatta gaattattac gtgggaaaag tggtagagta tttggtcaat
     6721 tatctggatt tttaatgtcg atcaaatcta ttccatctac ttataataaa gatatgcaag
     6781 aggataaaga accattattt gatgcattga ctacagtgga acattcaatt ttaattgcta
     6841 ctggtgtgat ttctacttta ctgattgata aacaaaatat ggaaaaagct ttaactatgg
     6901 atatgttggc tactgattta gctgattatt tagttagaaa aggagttcca tttagagaaa
     6961 ctcatcatat atctggtgaa tgtgttagaa aagctgaaga agaaaaatta tctggaattg
     7021 atcaattatc ttttgaacaa tttcaacaaa ttgattctcg ttttgaaaaa gacgttatgg
     7081 aaacttttga ttttgaagct agtgtggaaa gaagagatgc tattggtggt actgctaaaa
     7141 gtgccgtttt aaaacaatta gaaaatttaa aatcaatttt aagttaattt ataaatagtc
     7201 atataataat cacagtattg tttaacttac ataaaatata atcaattaac acagagatac
     7261 cttgtactcc aatactagtg ttataatgtt tataacagca gctttcaaga ataaggaact
     7321 aaataaaagc ttcttccatc acaacacaca acacgtctat atgccataaa tacataacgg
     7381 tcttcctaat aatatcctaa ccatacttgc aaatgcatg
//
