LOCUS       40924_13100        7268 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC81, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7268)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7268)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    124..394
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             855..884
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     rep_origin      complement(1449..2037)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2211..3068)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3069..3173)
                     /label=AmpR promoter
     regulatory      3244..3978
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             3979..4596
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             4618..4959
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      4977..5283
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     CDS             5630..7036
                     /codon_start=1
                     /product="Arg4"
                     /label=Arg4
                     /protein_id="AVR52661.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
ORIGIN
        1 cagtcagtca gtcaggcttg gacagcatca gcagtagcgt attgttcgaa tgaaccatcg
       61 tgagtgtaac cagacaagtc agcttcacca cagtttggtt cagcaccttg tcgagtttac
      121 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      181 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      241 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      301 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      361 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagc tccaaacttc
      421 ttttttattt ttcgcaatat tgtcgataaa ttaaatataa actactcgat tgagtaatag
      481 attcaataac tatgggaatt actaacaatg atttaaggaa aaagcttcac ttaaaaataa
      541 agccctttca attaaagtgt tgtccatgtt tgtcgtttct gatgggcttt tctgaaatat
      601 aaatatagat tcaaatctct tgtattcaag ttttagattt ttttttattt cttttatatc
      661 attatcaaat caaatcaaca aactctttca acttctcaga aagaaatcaa tatcaactac
      721 aataaaacca actacgtcta ctactacgtt aactcctaca cacatacaaa tataaatagt
      781 tattcatgtc cacacccgtt cccgggttaa ttaacggtga acaaaagcta atctccgagg
      841 aatacttgaa cggtgaacaa aaattaatct cagaagaaga cttgaacgga ctcgacggtg
      901 aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag
      961 acttgaacgg tgaacaaaag ttaatctcag aagaagactt gaacggactc gacggtgaac
     1021 aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaagact
     1081 tgaacggtga acaaaaatta atctcagaag aagacttgaa cggactcgac ggtgaacaaa
     1141 agttgatttc tgaagaagat ttgaacggtg aacaaaagct aatctccgag gaagacttga
     1201 acggtgaaca aaaattaatc tcagaagaag acttgaacgg actcgacggt gaacaaaagt
     1261 tgatttctga agaagatttg aacggtgaac aaaagctaat ctccgaggaa gacttgaacg
     1321 gtgaacaaaa attaatcaat cacgcatgcg attatagtct aagaactcga ggataacgca
     1381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1861 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2521 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3181 ccccgaaaag tgccacctga cgtctaagaa ccgcggaggc ctgggccccc gggtaccgag
     3241 ctcgccagcg agaaagagga gtattggcat tgttgggtag tagatgaatg agtaatcttc
     3301 tagccaaaac aaaataaacc acaagaacag taggcaaaac tgagtacttg atagcgcttg
     3361 tagtctttgt atttaaaaaa acctaattgt ccaaaaattg aaaatcatga gttgcaaatt
     3421 tgttgaaatc accaggtaaa tagatcattt agctatatcg ccttatcatt cttcgtacaa
     3481 gaattataca caaataccac caccacacca cccagaaatt ttacacgtgt tttctaaatt
     3541 attactttcc gtggccaatc aaattgaaat ttttcttgtt cgaaacggca gtgcctcaaa
     3601 cattatagta gcaagtattg cctaaagagt ttccgtctcg tgcccccccc ctatcccctt
     3661 ttgtaatttg ccaaaatagc tcaaaaagga taatcccgca cttttttttt ccgagttctc
     3721 gtcatgcaag caagcttatt cagaattttc agaggtgcga gtctcaccac taccactgca
     3781 gctgcatctt ttacagcaac agcaacagca ggtgccacca cggcaaagac attgtctgga
     3841 tccactgtgc tccgaaaact gtataaaagg acctattcat ccctggtctt atcttctcca
     3901 gaattatttt tttttcatca gtttaacaac aacaaacgtt attgtcatac aacaacaaca
     3961 acaaatacaa aaacaattat gtcaagatta gataaatcaa aagttattaa ttcagcttta
     4021 gaattattaa atggtgttgg tattgaaggt ttaactacta gaaaattagc tcaaaaatta
     4081 ggtgttgaac aaccaacttt atattggcat gttaaaaata aaagagcttt attagatgct
     4141 ttaccaattg aaatgttaga tagacatcat actcattcat gtccattaga aggtgaatca
     4201 tggcaagatt ttttaagaaa taatgctaaa tcatatagat gtgctttatt atcacataga
     4261 gatggtgcta aagttcattt aggtactaga ccaactgaaa aacaatatga aactttagaa
     4321 aatcaattag cttttttatg tcaacaaggt ttttcattag aaaatgcttt atatgcttta
     4381 tcagctgttg gtcattttac tttaggttgt gttttagaag aacaagaaca tcaagttgct
     4441 aaagaagaaa gagaaactcc aactactgat tcaatgccac cattattaaa acaagctatt
     4501 gaattatttg atagacaagg tgctgaacca gcttttttat ttggtttaga attaattatt
     4561 tgtggtttag aaaaacaatt aaaatgtgaa tcaggtggtt cgactttgtt tggtggcgcc
     4621 aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt
     4681 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt
     4741 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat
     4801 gatggtaata attcaaaacc attgtcacct ggttggacgg accaaactgc gtataacgcg
     4861 tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata taactatcta
     4921 ttcgatgatg aagatacccc accaaaccca aaaaaagagt aaaccgtgca tcgctaccat
     4981 tgttcaccac aaatgtttct aagagtgaaa ttctggaaat ctggaaatct ggttttgtat
     5041 tcttgttatt cttctttttg ttattacata tataacttgt tactttttta aaaaaatctt
     5101 tgtttatttt ataaatatat aaaactaaat ttaagaaaaa gagaaaaatg ttttatttga
     5161 gagattgaaa ttttacttga atttagctta gcttttataa agtattatta tgtaaaaaaa
     5221 caaaacaaat atacattaaa aagttaagac tataaaatag ccacccaagg catttctata
     5281 tctacagtct gccagactca atttatgaca tattgaccga cataattcag tcatttctaa
     5341 tattcaaata acaagacacc ttgtataaat gaatccacaa tcgtatatga acaaccaaat
     5401 tttatgacta actttgggtc gtgtactttt gacgattgag tcatttttac caattaggta
     5461 attgaatttt gttaatttgt cattaaccgc gctggccatc ccaacaataa caacaatcaa
     5521 tttaaaaact tgacaaggca aaaaaaaaaa aattttatct ttgaaaaatt tttcttcctt
     5581 ttcttcttct ggtctttaca taacagctat caagaataat caattaataa tgtcacaaca
     5641 acaagataaa caacctagtg aaaataaatt atggggtggt cgtttcaccg gtgccactga
     5701 tccattgatg gatttatata atgcttcatt accttatgat aaagttatgt atgatgctga
     5761 tttaactggt actaaagtat acacacaagg attaaacaaa ttaggattaa ttaccactga
     5821 agaattgcat ttgattcatc aaggattaga acaaatcaga caagaatggc atgataataa
     5881 attcattatt aaggcaggtg atgaggatat tcatactgct aatgaacgtc gattaggaga
     5941 aatcattggg aaaaacattt ctggtaaagt tcacactggt agatcaagaa atgatcaagt
     6001 tgctaccgat atgagaattt tcgttcgtga atcattactt aatttaagta aaattttaca
     6061 tcaattcatc acggcaattc ttgaacgggc acataaagaa atcgatgtat taatgcctgg
     6121 ttatactcat ttacaaaaag ctcaacctat tagatgggca cattggttaa gttcatatgc
     6181 aacttatttc accgaagatt ataaacgttt acaagaaatt ataactcgtg ttaaccaatc
     6241 tccattagga tctggtgctt tagcaggtca tccttatggt attgatcgtg aatttttagc
     6301 taaaggatta ggatttgatg gtgtcattgg gaattcttta actgcagttt cagatcgtga
     6361 ttttgttgtt gaaagtttat tttggagtac attatttatg aatcatattt ctcgattctc
     6421 agaagatcta attatttatt ctagtggtga atttgggttc ataaaattag ctgatgctta
     6481 ttctactggt tcttcattga tgccacaaaa gaaaaaccct gactcattag aattattacg
     6541 tgggaaaagt ggtagagtat ttggtcaatt atctggattt ttaatgtcga tcaaatctat
     6601 tccatctact tataataaag atatgcaaga ggataaagaa ccattatttg atgcattgac
     6661 tacagtggaa cattcaattt taattgctac tggtgtgatt tctactttac tgattgataa
     6721 acaaaatatg gaaaaagctt taactatgga tatgttggct actgatttag ctgattattt
     6781 agttagaaaa ggagttccat ttagagaaac tcatcatata tctggtgaat gtgttagaaa
     6841 agctgaagaa gaaaaattat ctggaattga tcaattatct tttgaacaat ttcaacaaat
     6901 tgattctcgt tttgaaaaag acgttatgga aacttttgat tttgaagcta gtgtggaaag
     6961 aagagatgct attggtggta ctgctaaaag tgccgtttta aaacaattag aaaatttaaa
     7021 atcaatttta agttaattta taaatagtca tataataatc acagtattgt ttaacttaca
     7081 taaaatataa tcaattaaca cagagatacc ttgtactcca atactagtgt tataatgttt
     7141 ataacagcag ctttcaagaa taaggaacta aataaaagct tcttccatca caacacacaa
     7201 cacgtctata tgccataaat acataacggt cttcctaata atatcctaac catacttgca
     7261 aatgcatg
//
