LOCUS       40924_13095        7372 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC80, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7372)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7372)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    124..394
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             828..854
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             858..884
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             888..914
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     rep_origin      complement(1553..2141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2315..3172)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3173..3277)
                     /label=AmpR promoter
     regulatory      3348..4082
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             4083..4700
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             4722..5063
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      5081..5387
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     CDS             5734..7140
                     /codon_start=1
                     /product="Arg4"
                     /label=Arg4
                     /protein_id="AVR52659.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
ORIGIN
        1 cagtcagtca gtcaggcttg gacagcatca gcagtagcgt attgttcgaa tgaaccatcg
       61 tgagtgtaac cagacaagtc agcttcacca cagtttggtt cagcaccttg tcgagtttac
      121 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      181 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      241 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      301 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      361 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagc tccaaacttc
      421 ttttttattt ttcgcaatat tgtcgataaa ttaaatataa actactcgat tgagtaatag
      481 attcaataac tatgggaatt actaacaatg atttaaggaa aaagcttcac ttaaaaataa
      541 agccctttca attaaagtgt tgtccatgtt tgtcgtttct gatgggcttt tctgaaatat
      601 aaatatagat tcaaatctct tgtattcaag ttttagattt ttttttattt cttttatatc
      661 attatcaaat caaatcaaca aactctttca acttctcaga aagaaatcaa tatcaactac
      721 aataaaacca actacgtcta ctactacgtt aactcctaca cacatacaaa tataaatagt
      781 tattcatgtc cacacgcaag ctcgcgagcg cgccaattag atccgtgtac ccatacgatg
      841 ttccagatta cgctggttac ccatacgatg ttccagatta cgctggttac ccatacgatg
      901 ttccagatta cgctatcgat atcccatgga gatctacgct aggatcgata tctcgagacg
      961 cgtgatcttg gacaccagta aataagcaaa tagctaaatc atatacgaat taatgttatg
     1021 attaagtgtt tacgtgagtg cgatattttt attactatct tatacagttg tatatactct
     1081 ataaaatgag ttgtctatta attaacgatg aatgctttct gggtttacct ctccaacgac
     1141 tctagtttac ttctcaatac attcaattgt atttgatttg tcaatacttc atcattaatc
     1201 aattctatag ttttgttttt ctcgtttatt tccaaattta atgcatcaat tttattattc
     1261 aatttgtcgt tgattttggt taatgatttt atggtttgat ctctggcatt gattgtttgt
     1321 gttagttttt cattattgat aattaaacta tttaagttag ttatcaactc ggtgttttca
     1381 agttttaagt tttcaatttc tttagagttt ataagatttg tcaaagtttc tgaattgctt
     1441 gattggtcct gtagaagcgt gtctaagaac tcgaggataa cgcaggaaag aacatgtgag
     1501 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1561 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1621 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1681 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1741 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1801 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1861 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1921 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1981 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2041 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2101 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2161 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2221 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2281 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2341 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2401 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2461 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2521 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2581 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2641 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2701 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2761 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2821 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2881 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2941 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3001 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3061 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3121 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3181 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3241 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3301 ctgacgtcta agaaccgcgg aggcctgggc ccccgggtac cgagctcgcc agcgagaaag
     3361 aggagtattg gcattgttgg gtagtagatg aatgagtaat cttctagcca aaacaaaata
     3421 aaccacaaga acagtaggca aaactgagta cttgatagcg cttgtagtct ttgtatttaa
     3481 aaaaacctaa ttgtccaaaa attgaaaatc atgagttgca aatttgttga aatcaccagg
     3541 taaatagatc atttagctat atcgccttat cattcttcgt acaagaatta tacacaaata
     3601 ccaccaccac accacccaga aattttacac gtgttttcta aattattact ttccgtggcc
     3661 aatcaaattg aaatttttct tgttcgaaac ggcagtgcct caaacattat agtagcaagt
     3721 attgcctaaa gagtttccgt ctcgtgcccc ccccctatcc ccttttgtaa tttgccaaaa
     3781 tagctcaaaa aggataatcc cgcacttttt ttttccgagt tctcgtcatg caagcaagct
     3841 tattcagaat tttcagaggt gcgagtctca ccactaccac tgcagctgca tcttttacag
     3901 caacagcaac agcaggtgcc accacggcaa agacattgtc tggatccact gtgctccgaa
     3961 aactgtataa aaggacctat tcatccctgg tcttatcttc tccagaatta tttttttttc
     4021 atcagtttaa caacaacaaa cgttattgtc atacaacaac aacaacaaat acaaaaacaa
     4081 ttatgtcaag attagataaa tcaaaagtta ttaattcagc tttagaatta ttaaatggtg
     4141 ttggtattga aggtttaact actagaaaat tagctcaaaa attaggtgtt gaacaaccaa
     4201 ctttatattg gcatgttaaa aataaaagag ctttattaga tgctttacca attgaaatgt
     4261 tagatagaca tcatactcat tcatgtccat tagaaggtga atcatggcaa gattttttaa
     4321 gaaataatgc taaatcatat agatgtgctt tattatcaca tagagatggt gctaaagttc
     4381 atttaggtac tagaccaact gaaaaacaat atgaaacttt agaaaatcaa ttagcttttt
     4441 tatgtcaaca aggtttttca ttagaaaatg ctttatatgc tttatcagct gttggtcatt
     4501 ttactttagg ttgtgtttta gaagaacaag aacatcaagt tgctaaagaa gaaagagaaa
     4561 ctccaactac tgattcaatg ccaccattat taaaacaagc tattgaatta tttgatagac
     4621 aaggtgctga accagctttt ttatttggtt tagaattaat tatttgtggt ttagaaaaac
     4681 aattaaaatg tgaatcaggt ggttcgactt tgtttggtgg cgccaatttt aatcaaagtg
     4741 ggaatattgc tgatagctca ttgtccttca ctttcactaa cagtagcaac ggtccgaacc
     4801 tcataacaac tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc tctaacgttc
     4861 atgataactt catgaataat gaaatcacgg ctagtaaaat tgatgatggt aataattcaa
     4921 aaccattgtc acctggttgg acggaccaaa ctgcgtataa cgcgtttgga atcactacag
     4981 ggatgtttaa taccactaca atggatgatg tatataacta tctattcgat gatgaagata
     5041 ccccaccaaa cccaaaaaaa gagtaaaccg tgcatcgcta ccattgttca ccacaaatgt
     5101 ttctaagagt gaaattctgg aaatctggaa atctggtttt gtattcttgt tattcttctt
     5161 tttgttatta catatataac ttgttacttt tttaaaaaaa tctttgttta ttttataaat
     5221 atataaaact aaatttaaga aaaagagaaa aatgttttat ttgagagatt gaaattttac
     5281 ttgaatttag cttagctttt ataaagtatt attatgtaaa aaaacaaaac aaatatacat
     5341 taaaaagtta agactataaa atagccaccc aaggcatttc tatatctaca gtctgccaga
     5401 ctcaatttat gacatattga ccgacataat tcagtcattt ctaatattca aataacaaga
     5461 caccttgtat aaatgaatcc acaatcgtat atgaacaacc aaattttatg actaactttg
     5521 ggtcgtgtac ttttgacgat tgagtcattt ttaccaatta ggtaattgaa ttttgttaat
     5581 ttgtcattaa ccgcgctggc catcccaaca ataacaacaa tcaatttaaa aacttgacaa
     5641 ggcaaaaaaa aaaaaatttt atctttgaaa aatttttctt ccttttcttc ttctggtctt
     5701 tacataacag ctatcaagaa taatcaatta ataatgtcac aacaacaaga taaacaacct
     5761 agtgaaaata aattatgggg tggtcgtttc accggtgcca ctgatccatt gatggattta
     5821 tataatgctt cattacctta tgataaagtt atgtatgatg ctgatttaac tggtactaaa
     5881 gtatacacac aaggattaaa caaattagga ttaattacca ctgaagaatt gcatttgatt
     5941 catcaaggat tagaacaaat cagacaagaa tggcatgata ataaattcat tattaaggca
     6001 ggtgatgagg atattcatac tgctaatgaa cgtcgattag gagaaatcat tgggaaaaac
     6061 atttctggta aagttcacac tggtagatca agaaatgatc aagttgctac cgatatgaga
     6121 attttcgttc gtgaatcatt acttaattta agtaaaattt tacatcaatt catcacggca
     6181 attcttgaac gggcacataa agaaatcgat gtattaatgc ctggttatac tcatttacaa
     6241 aaagctcaac ctattagatg ggcacattgg ttaagttcat atgcaactta tttcaccgaa
     6301 gattataaac gtttacaaga aattataact cgtgttaacc aatctccatt aggatctggt
     6361 gctttagcag gtcatcctta tggtattgat cgtgaatttt tagctaaagg attaggattt
     6421 gatggtgtca ttgggaattc tttaactgca gtttcagatc gtgattttgt tgttgaaagt
     6481 ttattttgga gtacattatt tatgaatcat atttctcgat tctcagaaga tctaattatt
     6541 tattctagtg gtgaatttgg gttcataaaa ttagctgatg cttattctac tggttcttca
     6601 ttgatgccac aaaagaaaaa ccctgactca ttagaattat tacgtgggaa aagtggtaga
     6661 gtatttggtc aattatctgg atttttaatg tcgatcaaat ctattccatc tacttataat
     6721 aaagatatgc aagaggataa agaaccatta tttgatgcat tgactacagt ggaacattca
     6781 attttaattg ctactggtgt gatttctact ttactgattg ataaacaaaa tatggaaaaa
     6841 gctttaacta tggatatgtt ggctactgat ttagctgatt atttagttag aaaaggagtt
     6901 ccatttagag aaactcatca tatatctggt gaatgtgtta gaaaagctga agaagaaaaa
     6961 ttatctggaa ttgatcaatt atcttttgaa caatttcaac aaattgattc tcgttttgaa
     7021 aaagacgtta tggaaacttt tgattttgaa gctagtgtgg aaagaagaga tgctattggt
     7081 ggtactgcta aaagtgccgt tttaaaacaa ttagaaaatt taaaatcaat tttaagttaa
     7141 tttataaata gtcatataat aatcacagta ttgtttaact tacataaaat ataatcaatt
     7201 aacacagaga taccttgtac tccaatacta gtgttataat gtttataaca gcagctttca
     7261 agaataagga actaaataaa agcttcttcc atcacaacac acaacacgtc tatatgccat
     7321 aaatacataa cggtcttcct aataatatcc taaccatact tgcaaatgca tg
//
