LOCUS       V008231                 7044 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008231
VERSION     V008231
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7044)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7044)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    201..471
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             872..1585
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     rep_origin      complement(1677..2265)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2439..3296)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3297..3401)
                     /label="AmpR promoter"
     regulatory      3472..4206
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             4207..4824
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             4846..5187
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      5205..5511
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     CDS             5973..6866
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
ORIGIN
        1 ttcgaccgtc atgtcctttc tataatgttt atctaacaaa gatgttgtgt aaaatgcagc
       61 gtagccatga gcaccatcag tcagtcagtc aggcttggac agcatcagca gtagcgtatt
      121 gttcgaatga accatcgtga gtgtaaccag acaagtcagc ttcaccacag tttggttcag
      181 caccttgtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      241 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      301 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      361 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
      421 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      481 gtcgagctcc aaacttcttt tttatttttc gcaatattgt cgataaatta aatataaact
      541 actcgattga gtaatagatt caataactat gggaattact aacaatgatt taaggaaaaa
      601 gcttcactta aaaataaagc cctttcaatt aaagtgttgt ccatgtttgt cgtttctgat
      661 gggcttttct gaaatataaa tatagattca aatctcttgt attcaagttt tagatttttt
      721 tttatttctt ttatatcatt atcaaatcaa atcaacaaac tctttcaact tctcagaaag
      781 aaatcaatat caactacaat aaaaccaact acgtctacta ctacgttaac tcctacacac
      841 atacaaatat aaatagttat tcatgtccac aatctctaaa ggtgaagaat tattcactgg
      901 tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc
      961 cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattta tttgtactac
     1021 tggtaaattg ccagttccat ggccaacctt agtcactact ttcggttatg gtgttcaatg
     1081 ttttgcgaga tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga
     1141 aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc
     1201 tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt
     1261 taaagaagat ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt
     1321 ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa
     1381 cattgaagat ggttctgttc aattagctga ccattatcaa caaaatactc caattggtga
     1441 tggtccagtc ttgttaccag acaaccatta cttatccact caatctgcct tatccaaaga
     1501 tccaaacgaa aagagagacc acatggtctt gttagaattt gttactgctg ctggtattac
     1561 ccatggtatg gatgaattgt acaagtctaa gaactcgagg ataacgcagg aaagaacatg
     1621 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1681 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1741 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1801 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1861 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1921 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1981 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2041 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2101 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2161 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2221 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2281 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2341 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2401 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2461 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2521 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2581 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2641 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2701 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2761 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2821 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2881 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     2941 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3001 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3061 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3121 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3181 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3241 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3301 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3361 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3421 ccacctgacg tctaagaacc gcggaggcct gggcccccgg gtaccgagct cgccagcgag
     3481 aaagaggagt attggcattg ttgggtagta gatgaatgag taatcttcta gccaaaacaa
     3541 aataaaccac aagaacagta ggcaaaactg agtacttgat agcgcttgta gtctttgtat
     3601 ttaaaaaaac ctaattgtcc aaaaattgaa aatcatgagt tgcaaatttg ttgaaatcac
     3661 caggtaaata gatcatttag ctatatcgcc ttatcattct tcgtacaaga attatacaca
     3721 aataccacca ccacaccacc cagaaatttt acacgtgttt tctaaattat tactttccgt
     3781 ggccaatcaa attgaaattt ttcttgttcg aaacggcagt gcctcaaaca ttatagtagc
     3841 aagtattgcc taaagagttt ccgtctcgtg ccccccccct atcccctttt gtaatttgcc
     3901 aaaatagctc aaaaaggata atcccgcact ttttttttcc gagttctcgt catgcaagca
     3961 agcttattca gaattttcag aggtgcgagt ctcaccacta ccactgcagc tgcatctttt
     4021 acagcaacag caacagcagg tgccaccacg gcaaagacat tgtctggatc cactgtgctc
     4081 cgaaaactgt ataaaaggac ctattcatcc ctggtcttat cttctccaga attatttttt
     4141 tttcatcagt ttaacaacaa caaacgttat tgtcatacaa caacaacaac aaatacaaaa
     4201 acaattatgt caagattaga taaatcaaaa gttattaatt cagctttaga attattaaat
     4261 ggtgttggta ttgaaggttt aactactaga aaattagctc aaaaattagg tgttgaacaa
     4321 ccaactttat attggcatgt taaaaataaa agagctttat tagatgcttt accaattgaa
     4381 atgttagata gacatcatac tcattcatgt ccattagaag gtgaatcatg gcaagatttt
     4441 ttaagaaata atgctaaatc atatagatgt gctttattat cacatagaga tggtgctaaa
     4501 gttcatttag gtactagacc aactgaaaaa caatatgaaa ctttagaaaa tcaattagct
     4561 tttttatgtc aacaaggttt ttcattagaa aatgctttat atgctttatc agctgttggt
     4621 cattttactt taggttgtgt tttagaagaa caagaacatc aagttgctaa agaagaaaga
     4681 gaaactccaa ctactgattc aatgccacca ttattaaaac aagctattga attatttgat
     4741 agacaaggtg ctgaaccagc ttttttattt ggtttagaat taattatttg tggtttagaa
     4801 aaacaattaa aatgtgaatc aggtggttcg actttgtttg gtggcgccaa ttttaatcaa
     4861 agtgggaata ttgctgatag ctcattgtcc ttcactttca ctaacagtag caacggtccg
     4921 aacctcataa caactcaaac aaattctcaa gcgctttcac aaccaattgc ctcctctaac
     4981 gttcatgata acttcatgaa taatgaaatc acggctagta aaattgatga tggtaataat
     5041 tcaaaaccat tgtcacctgg ttggacggac caaactgcgt ataacgcgtt tggaatcact
     5101 acagggatgt ttaataccac tacaatggat gatgtatata actatctatt cgatgatgaa
     5161 gataccccac caaacccaaa aaaagagtaa accgtgcatc gctaccattg ttcaccacaa
     5221 atgtttctaa gagtgaaatt ctggaaatct ggaaatctgg ttttgtattc ttgttattct
     5281 tctttttgtt attacatata taacttgtta cttttttaaa aaaatctttg tttattttat
     5341 aaatatataa aactaaattt aagaaaaaga gaaaaatgtt ttatttgaga gattgaaatt
     5401 ttacttgaat ttagcttagc ttttataaag tattattatg taaaaaaaca aaacaaatat
     5461 acattaaaaa gttaagacta taaaatagcc acccaaggca tttctatatc tacagtctgc
     5521 cagacttgta tggtaatcca aatgggtatg gggttagtag taatttggta gtgaaaaccc
     5581 cggtagacgg agaattaata aataccccaa aatccaccac aggactcgac aggtacctgg
     5641 aggatgagga gacagaagtt agtagtaaca atttggggac agaagaggat acagtgacat
     5701 cagaggaaga agacagcgaa ggtatagtca aaaagcaata ggacagcaag acaatttatt
     5761 tatttaatag ttttttataa atgtaaaatc tatcacaaca tgtgtactta attttctgtg
     5821 gtaggggagg ccatttcgtt gaaacgtgtc gcgagcggtt gtgagaattg actcattttg
     5881 attattacgc aaccattgaa attttttatt tttttttggt gaagattttt cccacacaac
     5941 ttcttctttt acttaaccac caactaccga taatggattt agtcaatcat ttaccagacc
     6001 gtttgttatt tgctgttcct aaaaagggca gattatacga aaaatgctgt aacttattga
     6061 gtggtgccga tatacagttt agaagatcta atagattaga tatagcactt tctacaaact
     6121 tgccaattgc attaatcttc ttgcctgcag ctgatatccc agttttcgtt ggagaaggta
     6181 attgtgactt gggtataact gggttagacc aaatcaaaga agctgaacaa ttcgacaaca
     6241 tcgaggactt gttggatttg aaatttggtt catgcaaatt gcagatccaa gttccagcag
     6301 atggcgagta cgaaaagcca gaacagcttg ttggaaagaa aattgtgtct tcatttacaa
     6361 aattgagtac cgactatttc aaacaattgt cagacaaacc tactaatatc agatatgtcg
     6421 gtggttccgt tgaggcttct tgtgccttgg gtgttgctga tgctattgtc gatttggttg
     6481 aaagtggtga aactatgaaa gcagctgggt taaaggccat agaaaccata ttggagactt
     6541 cggctcattt gatttcgtca aagaaaagta aattcccaga aatggtcaat ataatcgtcc
     6601 aaagacttca aggtgtttta gctgcccagg aatatgtctt gtgtaactac aatgccccaa
     6661 aatccatcca agcaaaatgt ttaaccatta ctccaggcag aagggccgct actgtctcta
     6721 ctttggacaa acacagcgac gacgaagagg actgggttgc catctcatcc atggttaata
     6781 gaaaggaaat cggtaatgta atggacgaat tgaagaaagc tggtgcaacc gatatattgg
     6841 tactcgagat atcaaattgt agagtttaaa agaagtgata gtttctcata aatattatac
     6901 atatatactt atagatctac ttcaatttga ctcaatctgt cttataccat ctttatctac
     6961 caccttgatg tacacacctt taaagtcaat aggcattctc gtttgtaatt ccttgacaca
     7021 catatccatt aactttaaac catc
//
