LOCUS       V008232                 6893 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008232
VERSION     V008232
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6893)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6893)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    201..471
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             932..961
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     rep_origin      complement(1526..2114)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2288..3145)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3146..3250)
                     /label="AmpR promoter"
     regulatory      3321..4055
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             4056..4673
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             4695..5036
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      5054..5360
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     CDS             5822..6715
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
ORIGIN
        1 ttcgaccgtc atgtcctttc tataatgttt atctaacaaa gatgttgtgt aaaatgcagc
       61 gtagccatga gcaccatcag tcagtcagtc aggcttggac agcatcagca gtagcgtatt
      121 gttcgaatga accatcgtga gtgtaaccag acaagtcagc ttcaccacag tttggttcag
      181 caccttgtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      241 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      301 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      361 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
      421 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      481 gtcgagctcc aaacttcttt tttatttttc gcaatattgt cgataaatta aatataaact
      541 actcgattga gtaatagatt caataactat gggaattact aacaatgatt taaggaaaaa
      601 gcttcactta aaaataaagc cctttcaatt aaagtgttgt ccatgtttgt cgtttctgat
      661 gggcttttct gaaatataaa tatagattca aatctcttgt attcaagttt tagatttttt
      721 tttatttctt ttatatcatt atcaaatcaa atcaacaaac tctttcaact tctcagaaag
      781 aaatcaatat caactacaat aaaaccaact acgtctacta ctacgttaac tcctacacac
      841 atacaaatat aaatagttat tcatgtccac acccgttccc gggttaatta acggtgaaca
      901 aaagctaatc tccgaggaat acttgaacgg tgaacaaaaa ttaatctcag aagaagactt
      961 gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa
     1021 gctaatctcc gaggaagact tgaacggtga acaaaagtta atctcagaag aagacttgaa
     1081 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct
     1141 aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg
     1201 actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat
     1261 ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact tgaacggact
     1321 cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
     1381 cgaggaagac ttgaacggtg aacaaaaatt aatcaatcac gcatgcgatt atagtctaag
     1441 aactcgagga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3241 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaccg cggaggcctg
     3301 ggcccccggg taccgagctc gccagcgaga aagaggagta ttggcattgt tgggtagtag
     3361 atgaatgagt aatcttctag ccaaaacaaa ataaaccaca agaacagtag gcaaaactga
     3421 gtacttgata gcgcttgtag tctttgtatt taaaaaaacc taattgtcca aaaattgaaa
     3481 atcatgagtt gcaaatttgt tgaaatcacc aggtaaatag atcatttagc tatatcgcct
     3541 tatcattctt cgtacaagaa ttatacacaa ataccaccac cacaccaccc agaaatttta
     3601 cacgtgtttt ctaaattatt actttccgtg gccaatcaaa ttgaaatttt tcttgttcga
     3661 aacggcagtg cctcaaacat tatagtagca agtattgcct aaagagtttc cgtctcgtgc
     3721 ccccccccta tccccttttg taatttgcca aaatagctca aaaaggataa tcccgcactt
     3781 tttttttccg agttctcgtc atgcaagcaa gcttattcag aattttcaga ggtgcgagtc
     3841 tcaccactac cactgcagct gcatctttta cagcaacagc aacagcaggt gccaccacgg
     3901 caaagacatt gtctggatcc actgtgctcc gaaaactgta taaaaggacc tattcatccc
     3961 tggtcttatc ttctccagaa ttattttttt ttcatcagtt taacaacaac aaacgttatt
     4021 gtcatacaac aacaacaaca aatacaaaaa caattatgtc aagattagat aaatcaaaag
     4081 ttattaattc agctttagaa ttattaaatg gtgttggtat tgaaggttta actactagaa
     4141 aattagctca aaaattaggt gttgaacaac caactttata ttggcatgtt aaaaataaaa
     4201 gagctttatt agatgcttta ccaattgaaa tgttagatag acatcatact cattcatgtc
     4261 cattagaagg tgaatcatgg caagattttt taagaaataa tgctaaatca tatagatgtg
     4321 ctttattatc acatagagat ggtgctaaag ttcatttagg tactagacca actgaaaaac
     4381 aatatgaaac tttagaaaat caattagctt ttttatgtca acaaggtttt tcattagaaa
     4441 atgctttata tgctttatca gctgttggtc attttacttt aggttgtgtt ttagaagaac
     4501 aagaacatca agttgctaaa gaagaaagag aaactccaac tactgattca atgccaccat
     4561 tattaaaaca agctattgaa ttatttgata gacaaggtgc tgaaccagct tttttatttg
     4621 gtttagaatt aattatttgt ggtttagaaa aacaattaaa atgtgaatca ggtggttcga
     4681 ctttgtttgg tggcgccaat tttaatcaaa gtgggaatat tgctgatagc tcattgtcct
     4741 tcactttcac taacagtagc aacggtccga acctcataac aactcaaaca aattctcaag
     4801 cgctttcaca accaattgcc tcctctaacg ttcatgataa cttcatgaat aatgaaatca
     4861 cggctagtaa aattgatgat ggtaataatt caaaaccatt gtcacctggt tggacggacc
     4921 aaactgcgta taacgcgttt ggaatcacta cagggatgtt taataccact acaatggatg
     4981 atgtatataa ctatctattc gatgatgaag ataccccacc aaacccaaaa aaagagtaaa
     5041 ccgtgcatcg ctaccattgt tcaccacaaa tgtttctaag agtgaaattc tggaaatctg
     5101 gaaatctggt tttgtattct tgttattctt ctttttgtta ttacatatat aacttgttac
     5161 ttttttaaaa aaatctttgt ttattttata aatatataaa actaaattta agaaaaagag
     5221 aaaaatgttt tatttgagag attgaaattt tacttgaatt tagcttagct tttataaagt
     5281 attattatgt aaaaaaacaa aacaaatata cattaaaaag ttaagactat aaaatagcca
     5341 cccaaggcat ttctatatct acagtctgcc agacttgtat ggtaatccaa atgggtatgg
     5401 ggttagtagt aatttggtag tgaaaacccc ggtagacgga gaattaataa ataccccaaa
     5461 atccaccaca ggactcgaca ggtacctgga ggatgaggag acagaagtta gtagtaacaa
     5521 tttggggaca gaagaggata cagtgacatc agaggaagaa gacagcgaag gtatagtcaa
     5581 aaagcaatag gacagcaaga caatttattt atttaatagt tttttataaa tgtaaaatct
     5641 atcacaacat gtgtacttaa ttttctgtgg taggggaggc catttcgttg aaacgtgtcg
     5701 cgagcggttg tgagaattga ctcattttga ttattacgca accattgaaa ttttttattt
     5761 ttttttggtg aagatttttc ccacacaact tcttctttta cttaaccacc aactaccgat
     5821 aatggattta gtcaatcatt taccagaccg tttgttattt gctgttccta aaaagggcag
     5881 attatacgaa aaatgctgta acttattgag tggtgccgat atacagttta gaagatctaa
     5941 tagattagat atagcacttt ctacaaactt gccaattgca ttaatcttct tgcctgcagc
     6001 tgatatccca gttttcgttg gagaaggtaa ttgtgacttg ggtataactg ggttagacca
     6061 aatcaaagaa gctgaacaat tcgacaacat cgaggacttg ttggatttga aatttggttc
     6121 atgcaaattg cagatccaag ttccagcaga tggcgagtac gaaaagccag aacagcttgt
     6181 tggaaagaaa attgtgtctt catttacaaa attgagtacc gactatttca aacaattgtc
     6241 agacaaacct actaatatca gatatgtcgg tggttccgtt gaggcttctt gtgccttggg
     6301 tgttgctgat gctattgtcg atttggttga aagtggtgaa actatgaaag cagctgggtt
     6361 aaaggccata gaaaccatat tggagacttc ggctcatttg atttcgtcaa agaaaagtaa
     6421 attcccagaa atggtcaata taatcgtcca aagacttcaa ggtgttttag ctgcccagga
     6481 atatgtcttg tgtaactaca atgccccaaa atccatccaa gcaaaatgtt taaccattac
     6541 tccaggcaga agggccgcta ctgtctctac tttggacaaa cacagcgacg acgaagagga
     6601 ctgggttgcc atctcatcca tggttaatag aaaggaaatc ggtaatgtaa tggacgaatt
     6661 gaagaaagct ggtgcaaccg atatattggt actcgagata tcaaattgta gagtttaaaa
     6721 gaagtgatag tttctcataa atattataca tatatactta tagatctact tcaatttgac
     6781 tcaatctgtc ttataccatc tttatctacc accttgatgt acacaccttt aaagtcaata
     6841 ggcattctcg tttgtaattc cttgacacac atatccatta actttaaacc atc
//
