LOCUS       40924_13075        6432 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC66, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6432)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6432)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(555..1412)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1413..1517)
                     /label=AmpR promoter
     CDS             1618..2328
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="ISKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELY"
     protein_bind    complement(6026..6059)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(6225..6432,1..381))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
       61 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      121 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      181 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
      241 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      301 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      361 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      421 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      481 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      541 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      601 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      661 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      721 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      781 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      841 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      901 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      961 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1021 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1081 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1141 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1201 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1261 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1321 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1381 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1441 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1501 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaactcgag
     1561 ccgcggaggc ctgggcccca ggtcgactct agatcgcgag ctagcacgcg tggtaccatc
     1621 tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat
     1681 gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa
     1741 ttgaccttaa aatttatttg tactactggt aaattgccag ttccatggcc aaccttagtc
     1801 actactttcg gttatggtgt tcaatgtttt gcgagatacc cagatcatat gaaacaacat
     1861 gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa
     1921 gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat
     1981 agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg
     2041 gaatacaact ataactctca caatgtttac atcatggctg acaaacaaaa gaatggtatc
     2101 aaagttaact tcaaaattag acacaacatt gaagatggtt ctgttcaatt agctgaccat
     2161 tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta
     2221 tccactcaat ctgccttatc caaagatcca aacgaaaaga gagaccacat ggtcttgtta
     2281 gaatttgtta ctgctgctgg tattacccat ggtatggatg aattgtacaa taactagata
     2341 gaccgtgcat cgctaccatt gttcaccaca aatgtttcta agagtgaaat tctggaaatc
     2401 tggaaatctg gttttgtatt cttgttattc ttctttttgt tattacatat ataacttgtt
     2461 acttttttaa aaaaatcttt gtttatttta taaatatata aaactaaatt taagaaaaag
     2521 agaaaaatgt tttatttgag agattgaaat tttacttgaa tttagcttag cttttataaa
     2581 gtattattat gtaaaaaaac aaaacaaata tacattaaaa agttaagact ataaaatagc
     2641 cacccaaggc atttctatat ctacagtctg ccagacttgc cacctgacgt ctaagaaaat
     2701 aacttcgtat agcatacatt atacgaagtt atagctcgga tccactagta acggccgcca
     2761 gtgtgctgga attcgccctt aagaacattt ctgtaccgca aatgtatcga ctttttcgat
     2821 ggtgtgttat tgttgttttg ttgcgaaata taatagcttc tgctttatat cctttgtgtt
     2881 gatccttccc ctcctttcat acttgtaaaa ttacaattgg tattttcaac caatattacc
     2941 acacgtcatg cactaactca aatgggcaaa aaaaagagag ataggaatta cgtcatttta
     3001 ttatgttttt acgtggacta gaaggttaag ataatttctg gttattttgt tgataattga
     3061 gttttgttcg acctccacca atctttaaac tctcgttgag tattcatttt aatgattttt
     3121 tgaaatcaga aggggtgttt ctaacccagg attgttttat tgagaggttg aaacatcaat
     3181 gtgttagttt attgatttgg gttggtttaa tagaaaaata tcagtaggtg ttcaataatt
     3241 gtagtttatt ttcttggcta aaattgttgc gaaaaattga caaaataata acaaaaagta
     3301 ttttagatgc taaaacataa tagtaggaat atctacctga accatttaca ttcaacaact
     3361 ggcaagaacc ttcctaataa tttgcaagag taatcacaat taaaccaaaa tcaaagttta
     3421 ccgtgtatat agttggacag aaagctgtca cattggttta atacatagtt gaatatgtat
     3481 gcatgaaaca acaacaatac aattacagct catgatcctt caattaattt caacattcaa
     3541 acaacataac accttgtgta aatgaggtta tgagtacatg tgaacaacaa atcttatgac
     3601 taaactcggg tcgtgtactt ttgacgattg agtcattttt accaattagg caattaattt
     3661 ttgctaaatt ttcattaacc gcgctggcca tcccaacaaa caatataaaa gctgacaacg
     3721 aaaaaaaaaa aaaaaaaaaa aaaactccaa ttctgaaaaa tttttcttct tcttcatact
     3781 aaacaaaaca actataaaga taatcaatta ataatgtcac aacaacctag tgaaaataaa
     3841 ttatggggtg gtcgattcac tggcgccact gatccattaa tggatttata caatgcttca
     3901 ttaccttatg ataaagttat gtatgatgca gatttaactg gtactaaagt atatactcaa
     3961 ggattgaaca aattaggatt aattaccact gaagaattga atctgattca tcaaggttta
     4021 gaacaaatta gacaagaatg gcatgataat aaatttatta ttaaagctgg tgatgaagat
     4081 attcatactg ctaatgaacg tagattagga gaaatcattg ggaaaaacat ttctggtaaa
     4141 gttcatactg gtagatccag aaatgatcaa gttgctactg atatgagaat ttttgttcgt
     4201 gaatcattac ttaatttaag taagatttta catcaattca ttacagcaat tcttgaacga
     4261 gctcataaag aaattgatgt attaatgcct ggttatactc atttacaaaa agctcaacct
     4321 attagatggg ctcattggtt aagttcatat gcaacttatt tcactgaaga ttataaacgt
     4381 ttacaagaaa tcattactcg tgttaatcaa tctccattag gatctggtgc tttagctggt
     4441 catccctacg gtattgatcg tgaattttta gctaaaggat taggatttga tggtgttatt
     4501 gggaattctt taactgcagt ttctgatcgt gattttgttg ttgaaagttt attttggagt
     4561 acattattta tgaatcatat ttctcgattt tcagaagatt taattattta ttctagtggt
     4621 gaatttggat ttataaagtt agctgatgct tactctactg gttcatcatt aatgccacaa
     4681 aagaaaaatc ctgattcatt agaattatta cgtgggaaaa gtggcagagt gtttggtcag
     4741 ttatctgggt ttttaatgtc aattaaatcc attccatcaa cctataataa agatatgcaa
     4801 gaagataaag aaccattatt tgatgcattg actaccgtag aacattcaat tttaattgct
     4861 actggtgtga tttctacttt actgattgat aaacaaaata tggaaaaagc tttaactatg
     4921 gatatgttag ctactgattt agcagattat ttagttagaa aaggtgttcc atttagagaa
     4981 actcatcata tttctggtga atgtgttaga aaagctgaag aagaaaaatt gtcgggaatt
     5041 gaccaattat catttgaaca atttcaacaa attgattcac gttttgaaaa agatgttatg
     5101 gaaacttttg attttgaagc tagtgtggaa agaagagatg ctattggtgg tactgctaag
     5161 agcgctgttt taaaacagtt agaaaatttg aaatcaattt taaattaatt tataaataat
     5221 catatagtat tcaatgtttt gttttgtttt tttttttttt tttatggaaa tgtacaaaca
     5281 gtatataatc aattaataga aagataccct gtattccatt gtatcgcctt tgggatgtct
     5341 ataataccaa ctttcaagaa tgagaaatta aacaacaatt tctttcacca atcaactcgt
     5401 ctatattcaa taaactcatt ctggtctttt caaatattta atgttatcct gatcaaactt
     5461 acaaatcaaa aatgtatcaa aatcattcat tagttcttct tcttttgtta tctttgtcac
     5521 tatatttctc tccatccaga ccaaaaatac ttccattgtg aagatcgtct gaatcataat
     5581 cagcaactac ctcttccatt tcagagaatt cagatttttc aaaataggta tcacgggcca
     5641 agtacacaca agacaaacga tacgagtgct gggatatttc tttcaatgta tcactaaatt
     5701 cgttgagatt taaagagagc cctttgactg ctttcattac tttcctcaca tcatgagcat
     5761 ccacattcaa atttcctgta gccctttatt cttagttttc ttttcttatt cttttcttcg
     5821 tacattgaaa tcacaaatgg tgatatcaaa cgaaggtcac actgacttat gtctaatacc
     5881 tccaatgatg atactggtaa cttaaccagg tctactcttt tcaaacgatt tctcattagt
     5941 gtcaatttac gtaggttctt caacctttca aacgatgcaa atagcttttg taagggcgaa
     6001 ttctgcagat atccatcaca ctggtataac ttcgtatagc atacattata cgaagttatg
     6061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6121 ctaactagat agcagtcagt cagtcaggat aacgcaggaa agaacatgtg agcaaaaggc
     6181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6421 agctcacgct gt
//
