LOCUS       V008235                 5487 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008235
VERSION     V008235
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5487)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5487)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(555..1412)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1413..1517)
                     /label="AmpR promoter"
     CDS             1618..2328
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     CDS             3808..4701
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(5081..5114)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(5280..5487,1..381))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
       61 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      121 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      181 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
      241 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      301 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      361 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      421 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      481 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      541 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      601 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      661 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      721 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      781 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      841 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      901 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      961 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1021 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1081 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1141 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1201 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1261 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1321 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1381 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1441 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1501 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaactcgag
     1561 ccgcggaggc ctgggcccca ggtcgactct agatcgcgag ctagcacgcg tggtaccatc
     1621 tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat
     1681 gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa
     1741 ttgaccttaa aatttatttg tactactggt aaattgccag ttccatggcc aaccttagtc
     1801 actactttcg gttatggtgt tcaatgtttt gcgagatacc cagatcatat gaaacaacat
     1861 gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa
     1921 gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat
     1981 agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg
     2041 gaatacaact ataactctca caatgtttac atcatggctg acaaacaaaa gaatggtatc
     2101 aaagttaact tcaaaattag acacaacatt gaagatggtt ctgttcaatt agctgaccat
     2161 tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta
     2221 tccactcaat ctgccttatc caaagatcca aacgaaaaga gagaccacat ggtcttgtta
     2281 gaatttgtta ctgctgctgg tattacccat ggtatggatg aattgtacaa taactagata
     2341 gaccgtgcat cgctaccatt gttcaccaca aatgtttcta agagtgaaat tctggaaatc
     2401 tggaaatctg gttttgtatt cttgttattc ttctttttgt tattacatat ataacttgtt
     2461 acttttttaa aaaaatcttt gtttatttta taaatatata aaactaaatt taagaaaaag
     2521 agaaaaatgt tttatttgag agattgaaat tttacttgaa tttagcttag cttttataaa
     2581 gtattattat gtaaaaaaac aaaacaaata tacattaaaa agttaagact ataaaatagc
     2641 cacccaaggc atttctatat ctacagtctg ccagacttgc cacctgacgt ctaagaaaat
     2701 aacttcgtat agcatacatt atacgaagtt atagctcgga tccactagta acggccgcca
     2761 gtgtgctgga attcgccctt gtttgatacc atggcaagca tactagaatt attatacgag
     2821 ctgggtgtga aatgtgccac cgtttatgca ttttcgatcg aaaacttcaa gagactgcaa
     2881 tatgaggtca aatggttgat ggaattagcc aaatccaagt ttacgcaaat aaaccaacat
     2941 ggattgttgt gtgaggagta tggggttaaa atcagaatat taggaaatac caaacttttg
     3001 ccgaaagatg tgttagagat ccttgaaaga accgaagaaa taactaaaaa caataagcgt
     3061 gctgtgttga atgtctgctt cccatataca tcaagagacg agataaccca tctgatcaag
     3121 tcagtagtag acgaagcgac gaaaggtggt attgaaataa ccgaggaggc tatcgacaag
     3181 catctttata cgaaagatgt gcccccattg gatttactag taagaacatc aggaacattt
     3241 agactttcag actttctttt atggcaaacg gtgtcgccag attgtgcagt tgtgtttgtc
     3301 gacaagttat ggccggcatt ctagaccatg ggatatgctc aagattttac tcaactgggg
     3361 gtttaacaaa tacatgtatg gaaacccaaa tggatatggg gttagtaata acttggtagt
     3421 gaagagccaa atggaagaag aattaataaa cacaccaaat ggagctacag ggcttgaccg
     3481 gtacctggag gacgaagaga ccgaagttag tagtaacaat ttaggaactg aagaggatac
     3541 agtgacatcc gaagaagaag atgttgaaga tataggcaaa aagtaatagg acagtatttt
     3601 atttattcaa tagttttttt tttttttttg aaacgtacac cttgtttatt tcaggtgttt
     3661 ctttgaaacg tgtctcaaag cgataacggg tacaaaaaaa aatgtattga gtcattgaaa
     3721 tttttttatt tttttttttt tttggtgaag atttttccca caccaaatat ataccacttt
     3781 tcttctaaca ccacctacat attcactatg gatttagtca atcatttgcc cgatcgtttg
     3841 ttatttgctg ttcccaaaaa gggcagatta tatgaaaaat gctgcagctt attgagcggt
     3901 gccgatatac agtttagaag atctaataga ttggatattg ccctttccac aaacttgcca
     3961 attgcattaa tctttttgcc tgcagccgat attccagttt tcgttggaga aggtaattgt
     4021 gacttgggta taactgggtt agaccagatc aaagaagcag accaattcga caacattgag
     4081 gatttgttgg atttgaagtt tggctcatgt aaattgcaga tccaagttcc tgcaaatggc
     4141 gagtacgaaa aacccgaaca actcgttgga aagaagattg tatcctcctt tacaaagttg
     4201 agtactgact atttcaagaa attatccgac aaacctacga atattagata cgttggtggt
     4261 tcagttgagg cttcttgcgc attgggtgtt gccgacgcca ttgtcgattt agttgaaagt
     4321 ggagagacca tgaaagctgc tggattaaag gcgatcgaaa ccatattgga aacttccgct
     4381 catttgattt cctccaagaa aaccaaattt ccagaaatgg tcaacataat tgtccaaaga
     4441 cttcaaggtg ttttagcggc tcaggaatat gtcttgtgta actacaatgc tccaaaatcc
     4501 attcaatcaa agtgcttaac cattactcca ggtagaaggg cagccactgt ctccactttg
     4561 gataaacaca gtgacgacga agatgactgg gttgccattt cttccatggt taacagaaag
     4621 gaaattggta atgtaatgga tgaattgaag aaagctggcg caacagatat attggtgctc
     4681 gagatatcaa attgtagagt ttaataaaat actatatata catacttata gatttacttc
     4741 aatttgactc aatctgtctt ataccatctt tatctaccac cttgatgtac acacctttaa
     4801 aatcaatggg cattctcgtt tgtaattctt tgacacacat gtccattaac ttcaaaccat
     4861 cttcaactgt catgtccttt ctataatgtt tatctaacaa ggatgttgtg taaaatgctg
     4921 cgtagccatg agcaccataa ggacgttcaa cttgcgtacc taaataatct atccaattca
     4981 acgacggctg atttgtcttt acatcgtatc ctccaattaa acagttcacc tggtacggtt
     5041 ttctagaagg gcgaattctg cagatatcca tcacactggt ataacttcgt atagcataca
     5101 ttatacgaag ttatggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5161 atgtgagcaa aaggcctaac tagatagcag tcagtcagtc aggataacgc aggaaagaac
     5221 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5281 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5341 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5401 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5461 gtggcgcttt ctcatagctc acgctgt
//
