LOCUS       V008236                 5406 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008236
VERSION     V008236
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5406)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5406)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(555..1412)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1413..1517)
                     /label="AmpR promoter"
     CDS             1618..2328
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     CDS             3629..4747
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(5000..5033)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(5199..5406,1..381))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
       61 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      121 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      181 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
      241 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      301 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      361 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      421 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      481 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      541 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      601 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      661 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      721 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      781 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      841 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      901 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      961 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1021 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1081 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1141 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1201 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1261 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1321 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1381 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1441 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1501 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaactcgag
     1561 ccgcggaggc ctgggcccca ggtcgactct agatcgcgag ctagcacgcg tggtaccatc
     1621 tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat
     1681 gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa
     1741 ttgaccttaa aatttatttg tactactggt aaattgccag ttccatggcc aaccttagtc
     1801 actactttcg gttatggtgt tcaatgtttt gcgagatacc cagatcatat gaaacaacat
     1861 gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa
     1921 gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat
     1981 agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg
     2041 gaatacaact ataactctca caatgtttac atcatggctg acaaacaaaa gaatggtatc
     2101 aaagttaact tcaaaattag acacaacatt gaagatggtt ctgttcaatt agctgaccat
     2161 tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta
     2221 tccactcaat ctgccttatc caaagatcca aacgaaaaga gagaccacat ggtcttgtta
     2281 gaatttgtta ctgctgctgg tattacccat ggtatggatg aattgtacaa taactagata
     2341 gaccgtgcat cgctaccatt gttcaccaca aatgtttcta agagtgaaat tctggaaatc
     2401 tggaaatctg gttttgtatt cttgttattc ttctttttgt tattacatat ataacttgtt
     2461 acttttttaa aaaaatcttt gtttatttta taaatatata aaactaaatt taagaaaaag
     2521 agaaaaatgt tttatttgag agattgaaat tttacttgaa tttagcttag cttttataaa
     2581 gtattattat gtaaaaaaac aaaacaaata tacattaaaa agttaagact ataaaatagc
     2641 cacccaaggc atttctatat ctacagtctg ccagacttgc cacctgacgt ctaagaaaat
     2701 aacttcgtat agcatacatt atacgaagtt atagctcgga tccactagta acggccgcca
     2761 gtgtgctgga attcgccctt ggatccaatc atcactggtg ggggtcaaga aagaggtttg
     2821 agatcaggta ctttggcacc accattagta gctgggtttg gtgaagctgc aagattaatg
     2881 aaacaagaat ctgcatttga caagaaacat attgagaggt tatctactaa attgaagaac
     2941 ggtttgttat ccattccatc tactcagttt aacggttgta ataacccaac atatcaatac
     3001 ccaggttgtg tgaatgtttc ctttgcatac attgaagggg aatctttgtt aatggcattg
     3061 aaagatattg cattgagttc tggttctgca tgtacctctg catctttgga accatcgtat
     3121 gttttacatg ctttgggtgc cgatgatgcc ttggctcatt cttccattag attcggtatt
     3181 ggtagattca ccactgaagc agaagttgac tatgttattc aagcaattaa tgaaagagtt
     3241 gatttcttga gaaagatgtc tccattatgg gaaatggttc aagaaggtat tgacttgaac
     3301 tctatcgaat ggagtggaca ttaaggtttt ttgttttttt gtaatttgtg ttgttatgtg
     3361 taattatcgt gtatagagta atttcggaaa ttgtactttc gttcatgtat ctgaaccaga
     3421 tgaataatac gttcgtattg ttgaagaatg actaactcct gtaacggcgt gcgtgggttc
     3481 cgcagccggg ttcaattttt tttttcttgt tcactataaa aatatataat aaacgaaaaa
     3541 aatccgaggg actcaggcta tgaaattttt cactcaagaa ttttttttcc ttcatcctta
     3601 attcctttat tcactaccca aacccacaat gtcagttaaa actaaaacta ttacaatttt
     3661 accaggtgat cacgtcggta ccgaaattgt caatgaagct atcaaagttt tagaagcaat
     3721 tgaagctgcc actccttacc aaaaaatcca tttcgatttc aaacatcatt taatcggtgg
     3781 tgctgccatt gatgccactg gtgtcccatt gccagatgac gcacttgaaa gtgccaaaaa
     3841 atctgatgcc gtcttattgg gtgctgttgg tggaccaaaa tgggggactg gcgccgttcg
     3901 tccagaacaa ggtttattaa agattcgtaa agaattgaac ctttatgcta acatcagacc
     3961 atgtaacttt gccagtgact ccttattgga attatcccca ttgagaccag aagtcgttaa
     4021 aggtacaaac ttgatcattg ttcgtgaatt agttggtggt atttatttcg gtgatcgtga
     4081 agaacaagaa gaaagtgaag ataaacaaac cgcatgggat actgaaaaat acaccgtcga
     4141 tgaagtcacc agaatcaccc gtatggctgc cttcatggct ttacaacata acccaccatt
     4201 accaatctgg tcattggata aggctaatgt tttagcttct tctagattat ggagaagaac
     4261 tgtcgataaa gtgatttctg aagagttccc aactttgtct gttcaacatc aattgattga
     4321 ctccgccgcc atgattttaa ttcaaaaccc aaccaaattg aatggtataa tcatcacttc
     4381 taacatgttt ggtgatatca tttccgatga agcttcagtt attccaggtt ccttgggttt
     4441 attaccatct gcatctttgg cttcattgcc agataccaac accgcttttg gtctttatga
     4501 accatgtcac ggttctgctc ctgatttacc agccaataaa gttaacccaa ttgctactat
     4561 tttatctgct gcttctatgt tgagattatc tttggattgt gtgaaagaag ctgaagcttt
     4621 agaagaagcc gtgaaacaag tcttggataa aggtatcaga actgcagatt taagaggtac
     4681 cagttcagct actgaagttg gtgacgcgat tgtcgaagct gttactaaaa tcttaaaaga
     4741 aaaagcttaa gtaaagtata cacaaaattt aacgaatatt attaatacaa aatgaacacc
     4801 acctaaatac ttttttttaa aaatgtaccg actcttctaa aaattgcgat aattcaatat
     4861 cggaaatttc tctcaccggt ttacttggat cttcggaaat aaatctattt gaagtaatgg
     4921 tagcctcact tgattctgat tggctctttc tagacatggg taggtaaggg cgaattctgc
     4981 agatatccat cacactggta taacttcgta tagcatacat tatacgaagt tatggtaata
     5041 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggcctaact
     5101 agatagcagt cagtcagtca ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5161 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5281 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5341 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5401 cgctgt
//
