LOCUS       40924_13060        6034 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC63, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6034)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6034)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(195..1052)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1053..1157)
                     /label=AmpR promoter
     CDS             1272..1298
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             1302..1328
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             1332..1358
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     protein_bind    complement(5268..5301)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(5467..6034,1..21))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
       61 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      121 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      181 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      241 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      301 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      361 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      421 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      481 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      541 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      601 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      661 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      721 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      781 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      841 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      901 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      961 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1021 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1141 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaactcgag
     1201 ccgcggaggc ctgggcccca ggtcgactct agaggtaccg ctagctcgcg agcgcgccaa
     1261 ttagatccgt gtacccatac gatgttccag attacgctgg ttacccatac gatgttccag
     1321 attacgctgg ttacccatac gatgttccag attacgctat cgatatccca tggagatcta
     1381 cgctaggatc gatatctcga gacgcgtgat cttggacacc agtaaataag caaatagcta
     1441 aatcatatac gaattaatgt tatgattaag tgtttacgtg agtgcgatat ttttattact
     1501 atcttataca gttgtatata ctctataaaa tgagttgtct attaattaac gatgaatgct
     1561 ttctgggttt acctctccaa cgactctagt ttacttctca atacattcaa ttgtatttga
     1621 tttgtcaata cttcatcatt aatcaattct atagttttgt ttttctcgtt tatttccaaa
     1681 tttaatgcat caattttatt attcaatttg tcgttgattt tggttaatga ttttatggtt
     1741 tgatctctgg cattgattgt ttgtgttagt ttttcattat tgataattaa actatttaag
     1801 ttagttatca actcggtgtt ttcaagtttt aagttttcaa tttctttaga gtttataaga
     1861 tttgtcaaag tttctgaatt gcttgattgg tcctgtagaa gcgtacagtc tgccagactt
     1921 gccacctgac gtctaagaaa ataacttcgt atagcataca ttatacgaag ttatagctcg
     1981 gatccactag taacggccgc cagtgtgctg gaattcgccc ttaagaacat ttctgtaccg
     2041 caaatgtatc gactttttcg atggtgtgtt attgttgttt tgttgcgaaa tataatagct
     2101 tctgctttat atcctttgtg ttgatccttc ccctcctttc atacttgtaa aattacaatt
     2161 ggtattttca accaatatta ccacacgtca tgcactaact caaatgggca aaaaaaagag
     2221 agataggaat tacgtcattt tattatgttt ttacgtggac tagaaggtta agataatttc
     2281 tggttatttt gttgataatt gagttttgtt cgacctccac caatctttaa actctcgttg
     2341 agtattcatt ttaatgattt tttgaaatca gaaggggtgt ttctaaccca ggattgtttt
     2401 attgagaggt tgaaacatca atgtgttagt ttattgattt gggttggttt aatagaaaaa
     2461 tatcagtagg tgttcaataa ttgtagttta ttttcttggc taaaattgtt gcgaaaaatt
     2521 gacaaaataa taacaaaaag tattttagat gctaaaacat aatagtagga atatctacct
     2581 gaaccattta cattcaacaa ctggcaagaa ccttcctaat aatttgcaag agtaatcaca
     2641 attaaaccaa aatcaaagtt taccgtgtat atagttggac agaaagctgt cacattggtt
     2701 taatacatag ttgaatatgt atgcatgaaa caacaacaat acaattacag ctcatgatcc
     2761 ttcaattaat ttcaacattc aaacaacata acaccttgtg taaatgaggt tatgagtaca
     2821 tgtgaacaac aaatcttatg actaaactcg ggtcgtgtac ttttgacgat tgagtcattt
     2881 ttaccaatta ggcaattaat ttttgctaaa ttttcattaa ccgcgctggc catcccaaca
     2941 aacaatataa aagctgacaa cgaaaaaaaa aaaaaaaaaa aaaaaactcc aattctgaaa
     3001 aatttttctt cttcttcata ctaaacaaaa caactataaa gataatcaat taataatgtc
     3061 acaacaacct agtgaaaata aattatgggg tggtcgattc actggcgcca ctgatccatt
     3121 aatggattta tacaatgctt cattacctta tgataaagtt atgtatgatg cagatttaac
     3181 tggtactaaa gtatatactc aaggattgaa caaattagga ttaattacca ctgaagaatt
     3241 gaatctgatt catcaaggtt tagaacaaat tagacaagaa tggcatgata ataaatttat
     3301 tattaaagct ggtgatgaag atattcatac tgctaatgaa cgtagattag gagaaatcat
     3361 tgggaaaaac atttctggta aagttcatac tggtagatcc agaaatgatc aagttgctac
     3421 tgatatgaga atttttgttc gtgaatcatt acttaattta agtaagattt tacatcaatt
     3481 cattacagca attcttgaac gagctcataa agaaattgat gtattaatgc ctggttatac
     3541 tcatttacaa aaagctcaac ctattagatg ggctcattgg ttaagttcat atgcaactta
     3601 tttcactgaa gattataaac gtttacaaga aatcattact cgtgttaatc aatctccatt
     3661 aggatctggt gctttagctg gtcatcccta cggtattgat cgtgaatttt tagctaaagg
     3721 attaggattt gatggtgtta ttgggaattc tttaactgca gtttctgatc gtgattttgt
     3781 tgttgaaagt ttattttgga gtacattatt tatgaatcat atttctcgat tttcagaaga
     3841 tttaattatt tattctagtg gtgaatttgg atttataaag ttagctgatg cttactctac
     3901 tggttcatca ttaatgccac aaaagaaaaa tcctgattca ttagaattat tacgtgggaa
     3961 aagtggcaga gtgtttggtc agttatctgg gtttttaatg tcaattaaat ccattccatc
     4021 aacctataat aaagatatgc aagaagataa agaaccatta tttgatgcat tgactaccgt
     4081 agaacattca attttaattg ctactggtgt gatttctact ttactgattg ataaacaaaa
     4141 tatggaaaaa gctttaacta tggatatgtt agctactgat ttagcagatt atttagttag
     4201 aaaaggtgtt ccatttagag aaactcatca tatttctggt gaatgtgtta gaaaagctga
     4261 agaagaaaaa ttgtcgggaa ttgaccaatt atcatttgaa caatttcaac aaattgattc
     4321 acgttttgaa aaagatgtta tggaaacttt tgattttgaa gctagtgtgg aaagaagaga
     4381 tgctattggt ggtactgcta agagcgctgt tttaaaacag ttagaaaatt tgaaatcaat
     4441 tttaaattaa tttataaata atcatatagt attcaatgtt ttgttttgtt tttttttttt
     4501 tttttatgga aatgtacaaa cagtatataa tcaattaata gaaagatacc ctgtattcca
     4561 ttgtatcgcc tttgggatgt ctataatacc aactttcaag aatgagaaat taaacaacaa
     4621 tttctttcac caatcaactc gtctatattc aataaactca ttctggtctt ttcaaatatt
     4681 taatgttatc ctgatcaaac ttacaaatca aaaatgtatc aaaatcattc attagttctt
     4741 cttcttttgt tatctttgtc actatatttc tctccatcca gaccaaaaat acttccattg
     4801 tgaagatcgt ctgaatcata atcagcaact acctcttcca tttcagagaa ttcagatttt
     4861 tcaaaatagg tatcacgggc caagtacaca caagacaaac gatacgagtg ctgggatatt
     4921 tctttcaatg tatcactaaa ttcgttgaga tttaaagaga gccctttgac tgctttcatt
     4981 actttcctca catcatgagc atccacattc aaatttcctg tagcccttta ttcttagttt
     5041 tcttttctta ttcttttctt cgtacattga aatcacaaat ggtgatatca aacgaaggtc
     5101 acactgactt atgtctaata cctccaatga tgatactggt aacttaacca ggtctactct
     5161 tttcaaacga tttctcatta gtgtcaattt acgtaggttc ttcaaccttt caaacgatgc
     5221 aaatagcttt tgtaagggcg aattctgcag atatccatca cactggtata acttcgtata
     5281 gcatacatta tacgaagtta tggtaatacg gttatccaca gaatcagggg ataacgcagg
     5341 aaagaacatg tgagcaaaag gcctaactag atagcagtca gtcagtcagg ataacgcagg
     5401 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5461 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5521 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5581 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5641 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5701 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5761 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5821 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5881 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5941 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6001 cggtggtttt tttgtttgca agcagcagat tacg
//
