LOCUS       V008238                 5089 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008238
VERSION     V008238
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5089)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5089)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(195..1052)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1053..1157)
                     /label="AmpR promoter"
     CDS             1272..1298
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1302..1328
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1332..1358
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3050..3943
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(4323..4356)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(4522..5089,1..21))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
       61 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      121 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      181 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      241 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      301 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      361 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      421 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      481 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      541 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      601 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      661 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      721 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      781 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      841 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      901 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      961 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1021 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1141 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaactcgag
     1201 ccgcggaggc ctgggcccca ggtcgactct agaggtaccg ctagctcgcg agcgcgccaa
     1261 ttagatccgt gtacccatac gatgttccag attacgctgg ttacccatac gatgttccag
     1321 attacgctgg ttacccatac gatgttccag attacgctat cgatatccca tggagatcta
     1381 cgctaggatc gatatctcga gacgcgtgat cttggacacc agtaaataag caaatagcta
     1441 aatcatatac gaattaatgt tatgattaag tgtttacgtg agtgcgatat ttttattact
     1501 atcttataca gttgtatata ctctataaaa tgagttgtct attaattaac gatgaatgct
     1561 ttctgggttt acctctccaa cgactctagt ttacttctca atacattcaa ttgtatttga
     1621 tttgtcaata cttcatcatt aatcaattct atagttttgt ttttctcgtt tatttccaaa
     1681 tttaatgcat caattttatt attcaatttg tcgttgattt tggttaatga ttttatggtt
     1741 tgatctctgg cattgattgt ttgtgttagt ttttcattat tgataattaa actatttaag
     1801 ttagttatca actcggtgtt ttcaagtttt aagttttcaa tttctttaga gtttataaga
     1861 tttgtcaaag tttctgaatt gcttgattgg tcctgtagaa gcgtacagtc tgccagactt
     1921 gccacctgac gtctaagaaa ataacttcgt atagcataca ttatacgaag ttatagctcg
     1981 gatccactag taacggccgc cagtgtgctg gaattcgccc ttgtttgata ccatggcaag
     2041 catactagaa ttattatacg agctgggtgt gaaatgtgcc accgtttatg cattttcgat
     2101 cgaaaacttc aagagactgc aatatgaggt caaatggttg atggaattag ccaaatccaa
     2161 gtttacgcaa ataaaccaac atggattgtt gtgtgaggag tatggggtta aaatcagaat
     2221 attaggaaat accaaacttt tgccgaaaga tgtgttagag atccttgaaa gaaccgaaga
     2281 aataactaaa aacaataagc gtgctgtgtt gaatgtctgc ttcccatata catcaagaga
     2341 cgagataacc catctgatca agtcagtagt agacgaagcg acgaaaggtg gtattgaaat
     2401 aaccgaggag gctatcgaca agcatcttta tacgaaagat gtgcccccat tggatttact
     2461 agtaagaaca tcaggaacat ttagactttc agactttctt ttatggcaaa cggtgtcgcc
     2521 agattgtgca gttgtgtttg tcgacaagtt atggccggca ttctagacca tgggatatgc
     2581 tcaagatttt actcaactgg gggtttaaca aatacatgta tggaaaccca aatggatatg
     2641 gggttagtaa taacttggta gtgaagagcc aaatggaaga agaattaata aacacaccaa
     2701 atggagctac agggcttgac cggtacctgg aggacgaaga gaccgaagtt agtagtaaca
     2761 atttaggaac tgaagaggat acagtgacat ccgaagaaga agatgttgaa gatataggca
     2821 aaaagtaata ggacagtatt ttatttattc aatagttttt tttttttttt tgaaacgtac
     2881 accttgttta tttcaggtgt ttctttgaaa cgtgtctcaa agcgataacg ggtacaaaaa
     2941 aaaatgtatt gagtcattga aattttttta tttttttttt tttttggtga agatttttcc
     3001 cacaccaaat atataccact tttcttctaa caccacctac atattcacta tggatttagt
     3061 caatcatttg cccgatcgtt tgttatttgc tgttcccaaa aagggcagat tatatgaaaa
     3121 atgctgcagc ttattgagcg gtgccgatat acagtttaga agatctaata gattggatat
     3181 tgccctttcc acaaacttgc caattgcatt aatctttttg cctgcagccg atattccagt
     3241 tttcgttgga gaaggtaatt gtgacttggg tataactggg ttagaccaga tcaaagaagc
     3301 agaccaattc gacaacattg aggatttgtt ggatttgaag tttggctcat gtaaattgca
     3361 gatccaagtt cctgcaaatg gcgagtacga aaaacccgaa caactcgttg gaaagaagat
     3421 tgtatcctcc tttacaaagt tgagtactga ctatttcaag aaattatccg acaaacctac
     3481 gaatattaga tacgttggtg gttcagttga ggcttcttgc gcattgggtg ttgccgacgc
     3541 cattgtcgat ttagttgaaa gtggagagac catgaaagct gctggattaa aggcgatcga
     3601 aaccatattg gaaacttccg ctcatttgat ttcctccaag aaaaccaaat ttccagaaat
     3661 ggtcaacata attgtccaaa gacttcaagg tgttttagcg gctcaggaat atgtcttgtg
     3721 taactacaat gctccaaaat ccattcaatc aaagtgctta accattactc caggtagaag
     3781 ggcagccact gtctccactt tggataaaca cagtgacgac gaagatgact gggttgccat
     3841 ttcttccatg gttaacagaa aggaaattgg taatgtaatg gatgaattga agaaagctgg
     3901 cgcaacagat atattggtgc tcgagatatc aaattgtaga gtttaataaa atactatata
     3961 tacatactta tagatttact tcaatttgac tcaatctgtc ttataccatc tttatctacc
     4021 accttgatgt acacaccttt aaaatcaatg ggcattctcg tttgtaattc tttgacacac
     4081 atgtccatta acttcaaacc atcttcaact gtcatgtcct ttctataatg tttatctaac
     4141 aaggatgttg tgtaaaatgc tgcgtagcca tgagcaccat aaggacgttc aacttgcgta
     4201 cctaaataat ctatccaatt caacgacggc tgatttgtct ttacatcgta tcctccaatt
     4261 aaacagttca cctggtacgg ttttctagaa gggcgaattc tgcagatatc catcacactg
     4321 gtataacttc gtatagcata cattatacga agttatggta atacggttat ccacagaatc
     4381 aggggataac gcaggaaaga acatgtgagc aaaaggccta actagatagc agtcagtcag
     4441 tcaggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4921 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacg
//
