LOCUS       40924_13045        6279 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC60, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6279)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6279)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(513..617)
                     /label=AmpR promoter
     CDS             776..805
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     protein_bind    complement(4973..5006)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(5172..5760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5934..6279,1..512))
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
       61 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      121 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      181 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      241 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      301 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      361 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      421 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      481 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      541 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
      601 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaactcgag
      661 ccgcggaggc ctgggcccca ggtcgactct agatcgcgag ctagcacgcg tggtacccgt
      721 tcccgggtta attaacggtg aacaaaagct aatctccgag gaatacttga acggtgaaca
      781 aaaattaatc tcagaagaag acttgaacgg actcgacggt gaacaaaagt tgatttctga
      841 agaagatttg aacggtgaac aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa
      901 gttaatctca gaagaagact tgaacggact cgacggtgaa caaaagttga tttctgaaga
      961 agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg aacaaaaatt
     1021 aatctcagaa gaagacttga acggactcga cggtgaacaa aagttgattt ctgaagaaga
     1081 tttgaacggt gaacaaaagc taatctccga ggaagacttg aacggtgaac aaaaattaat
     1141 ctcagaagaa gacttgaacg gactcgacgg tgaacaaaag ttgatttctg aagaagattt
     1201 gaacggtgaa caaaagctaa tctccgagga agacttgaac ggtgaacaaa aattaatcaa
     1261 tcacgcatgc gattatataa ctagatagac cgtgcatcgc taccattgtt caccacaaat
     1321 gtttctaaga gtgaaattct ggaaatctgg aaatctggtt ttgtattctt gttattcttc
     1381 tttttgttat tacatatata acttgttact tttttaaaaa aatctttgtt tattttataa
     1441 atatataaaa ctaaatttaa gaaaaagaga aaaatgtttt atttgagaga ttgaaatttt
     1501 acttgaattt agcttagctt ttataaagta ttattatgta aaaaaacaaa acaaatatac
     1561 attaaaaagt taagactata aaatagccac ccaaggcatt tctatatcta cagtctgcca
     1621 gacttgccac ctgacgtcta agaaaataac ttcgtatagc atacattata cgaagttata
     1681 gctcggatcc actagtaacg gccgccagtg tgctggaatt cgcccttaag aacatttctg
     1741 taccgcaaat gtatcgactt tttcgatggt gtgttattgt tgttttgttg cgaaatataa
     1801 tagcttctgc tttatatcct ttgtgttgat ccttcccctc ctttcatact tgtaaaatta
     1861 caattggtat tttcaaccaa tattaccaca cgtcatgcac taactcaaat gggcaaaaaa
     1921 aagagagata ggaattacgt cattttatta tgtttttacg tggactagaa ggttaagata
     1981 atttctggtt attttgttga taattgagtt ttgttcgacc tccaccaatc tttaaactct
     2041 cgttgagtat tcattttaat gattttttga aatcagaagg ggtgtttcta acccaggatt
     2101 gttttattga gaggttgaaa catcaatgtg ttagtttatt gatttgggtt ggtttaatag
     2161 aaaaatatca gtaggtgttc aataattgta gtttattttc ttggctaaaa ttgttgcgaa
     2221 aaattgacaa aataataaca aaaagtattt tagatgctaa aacataatag taggaatatc
     2281 tacctgaacc atttacattc aacaactggc aagaaccttc ctaataattt gcaagagtaa
     2341 tcacaattaa accaaaatca aagtttaccg tgtatatagt tggacagaaa gctgtcacat
     2401 tggtttaata catagttgaa tatgtatgca tgaaacaaca acaatacaat tacagctcat
     2461 gatccttcaa ttaatttcaa cattcaaaca acataacacc ttgtgtaaat gaggttatga
     2521 gtacatgtga acaacaaatc ttatgactaa actcgggtcg tgtacttttg acgattgagt
     2581 catttttacc aattaggcaa ttaatttttg ctaaattttc attaaccgcg ctggccatcc
     2641 caacaaacaa tataaaagct gacaacgaaa aaaaaaaaaa aaaaaaaaaa actccaattc
     2701 tgaaaaattt ttcttcttct tcatactaaa caaaacaact ataaagataa tcaattaata
     2761 atgtcacaac aacctagtga aaataaatta tggggtggtc gattcactgg cgccactgat
     2821 ccattaatgg atttatacaa tgcttcatta ccttatgata aagttatgta tgatgcagat
     2881 ttaactggta ctaaagtata tactcaagga ttgaacaaat taggattaat taccactgaa
     2941 gaattgaatc tgattcatca aggtttagaa caaattagac aagaatggca tgataataaa
     3001 tttattatta aagctggtga tgaagatatt catactgcta atgaacgtag attaggagaa
     3061 atcattggga aaaacatttc tggtaaagtt catactggta gatccagaaa tgatcaagtt
     3121 gctactgata tgagaatttt tgttcgtgaa tcattactta atttaagtaa gattttacat
     3181 caattcatta cagcaattct tgaacgagct cataaagaaa ttgatgtatt aatgcctggt
     3241 tatactcatt tacaaaaagc tcaacctatt agatgggctc attggttaag ttcatatgca
     3301 acttatttca ctgaagatta taaacgttta caagaaatca ttactcgtgt taatcaatct
     3361 ccattaggat ctggtgcttt agctggtcat ccctacggta ttgatcgtga atttttagct
     3421 aaaggattag gatttgatgg tgttattggg aattctttaa ctgcagtttc tgatcgtgat
     3481 tttgttgttg aaagtttatt ttggagtaca ttatttatga atcatatttc tcgattttca
     3541 gaagatttaa ttatttattc tagtggtgaa tttggattta taaagttagc tgatgcttac
     3601 tctactggtt catcattaat gccacaaaag aaaaatcctg attcattaga attattacgt
     3661 gggaaaagtg gcagagtgtt tggtcagtta tctgggtttt taatgtcaat taaatccatt
     3721 ccatcaacct ataataaaga tatgcaagaa gataaagaac cattatttga tgcattgact
     3781 accgtagaac attcaatttt aattgctact ggtgtgattt ctactttact gattgataaa
     3841 caaaatatgg aaaaagcttt aactatggat atgttagcta ctgatttagc agattattta
     3901 gttagaaaag gtgttccatt tagagaaact catcatattt ctggtgaatg tgttagaaaa
     3961 gctgaagaag aaaaattgtc gggaattgac caattatcat ttgaacaatt tcaacaaatt
     4021 gattcacgtt ttgaaaaaga tgttatggaa acttttgatt ttgaagctag tgtggaaaga
     4081 agagatgcta ttggtggtac tgctaagagc gctgttttaa aacagttaga aaatttgaaa
     4141 tcaattttaa attaatttat aaataatcat atagtattca atgttttgtt ttgttttttt
     4201 tttttttttt atggaaatgt acaaacagta tataatcaat taatagaaag ataccctgta
     4261 ttccattgta tcgcctttgg gatgtctata ataccaactt tcaagaatga gaaattaaac
     4321 aacaatttct ttcaccaatc aactcgtcta tattcaataa actcattctg gtcttttcaa
     4381 atatttaatg ttatcctgat caaacttaca aatcaaaaat gtatcaaaat cattcattag
     4441 ttcttcttct tttgttatct ttgtcactat atttctctcc atccagacca aaaatacttc
     4501 cattgtgaag atcgtctgaa tcataatcag caactacctc ttccatttca gagaattcag
     4561 atttttcaaa ataggtatca cgggccaagt acacacaaga caaacgatac gagtgctggg
     4621 atatttcttt caatgtatca ctaaattcgt tgagatttaa agagagccct ttgactgctt
     4681 tcattacttt cctcacatca tgagcatcca cattcaaatt tcctgtagcc ctttattctt
     4741 agttttcttt tcttattctt ttcttcgtac attgaaatca caaatggtga tatcaaacga
     4801 aggtcacact gacttatgtc taatacctcc aatgatgata ctggtaactt aaccaggtct
     4861 actcttttca aacgatttct cattagtgtc aatttacgta ggttcttcaa cctttcaaac
     4921 gatgcaaata gcttttgtaa gggcgaattc tgcagatatc catcacactg gtataacttc
     4981 gtatagcata cattatacga agttatggta atacggttat ccacagaatc aggggataac
     5041 gcaggaaaga acatgtgagc aaaaggccta actagatagc agtcagtcag tcaggataac
     5101 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5161 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5221 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5281 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5341 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5401 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5461 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5521 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5581 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5641 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5701 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5761 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5821 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5881 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5941 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6001 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6061 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6121 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6181 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6241 attgctacag gcatcgtggt gtcacgctcg tcgtttggt
//
