LOCUS       V008241                 5334 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008241
VERSION     V008241
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5334)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5334)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(277..1134)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1135..1239)
                     /label="AmpR promoter"
     CDS             1398..1427
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3377..4270
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(4650..4683)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(4849..5334,1..103))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
       61 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      181 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      241 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      301 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      361 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
      421 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      481 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      541 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      601 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      661 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      721 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      781 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      841 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      901 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      961 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1021 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1081 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1141 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1201 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1261 acctgacgtc taagaactcg agccgcggag gcctgggccc caggtcgact ctagatcgcg
     1321 agctagcacg cgtggtaccc gttcccgggt taattaacgg tgaacaaaag ctaatctccg
     1381 aggaatactt gaacggtgaa caaaaattaa tctcagaaga agacttgaac ggactcgacg
     1441 gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta atctccgagg
     1501 aagacttgaa cggtgaacaa aagttaatct cagaagaaga cttgaacgga ctcgacggtg
     1561 aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag
     1621 acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggactc gacggtgaac
     1681 aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaagact
     1741 tgaacggtga acaaaaatta atctcagaag aagacttgaa cggactcgac ggtgaacaaa
     1801 agttgatttc tgaagaagat ttgaacggtg aacaaaagct aatctccgag gaagacttga
     1861 acggtgaaca aaaattaatc aatcacgcat gcgattatat aactagatag accgtgcatc
     1921 gctaccattg ttcaccacaa atgtttctaa gagtgaaatt ctggaaatct ggaaatctgg
     1981 ttttgtattc ttgttattct tctttttgtt attacatata taacttgtta cttttttaaa
     2041 aaaatctttg tttattttat aaatatataa aactaaattt aagaaaaaga gaaaaatgtt
     2101 ttatttgaga gattgaaatt ttacttgaat ttagcttagc ttttataaag tattattatg
     2161 taaaaaaaca aaacaaatat acattaaaaa gttaagacta taaaatagcc acccaaggca
     2221 tttctatatc tacagtctgc cagacttgcc acctgacgtc taagaaaata acttcgtata
     2281 gcatacatta tacgaagtta tagctcggat ccactagtaa cggccgccag tgtgctggaa
     2341 ttcgcccttg tttgatacca tggcaagcat actagaatta ttatacgagc tgggtgtgaa
     2401 atgtgccacc gtttatgcat tttcgatcga aaacttcaag agactgcaat atgaggtcaa
     2461 atggttgatg gaattagcca aatccaagtt tacgcaaata aaccaacatg gattgttgtg
     2521 tgaggagtat ggggttaaaa tcagaatatt aggaaatacc aaacttttgc cgaaagatgt
     2581 gttagagatc cttgaaagaa ccgaagaaat aactaaaaac aataagcgtg ctgtgttgaa
     2641 tgtctgcttc ccatatacat caagagacga gataacccat ctgatcaagt cagtagtaga
     2701 cgaagcgacg aaaggtggta ttgaaataac cgaggaggct atcgacaagc atctttatac
     2761 gaaagatgtg cccccattgg atttactagt aagaacatca ggaacattta gactttcaga
     2821 ctttctttta tggcaaacgg tgtcgccaga ttgtgcagtt gtgtttgtcg acaagttatg
     2881 gccggcattc tagaccatgg gatatgctca agattttact caactggggg tttaacaaat
     2941 acatgtatgg aaacccaaat ggatatgggg ttagtaataa cttggtagtg aagagccaaa
     3001 tggaagaaga attaataaac acaccaaatg gagctacagg gcttgaccgg tacctggagg
     3061 acgaagagac cgaagttagt agtaacaatt taggaactga agaggataca gtgacatccg
     3121 aagaagaaga tgttgaagat ataggcaaaa agtaatagga cagtatttta tttattcaat
     3181 agtttttttt ttttttttga aacgtacacc ttgtttattt caggtgtttc tttgaaacgt
     3241 gtctcaaagc gataacgggt acaaaaaaaa atgtattgag tcattgaaat ttttttattt
     3301 tttttttttt ttggtgaaga tttttcccac accaaatata taccactttt cttctaacac
     3361 cacctacata ttcactatgg atttagtcaa tcatttgccc gatcgtttgt tatttgctgt
     3421 tcccaaaaag ggcagattat atgaaaaatg ctgcagctta ttgagcggtg ccgatataca
     3481 gtttagaaga tctaatagat tggatattgc cctttccaca aacttgccaa ttgcattaat
     3541 ctttttgcct gcagccgata ttccagtttt cgttggagaa ggtaattgtg acttgggtat
     3601 aactgggtta gaccagatca aagaagcaga ccaattcgac aacattgagg atttgttgga
     3661 tttgaagttt ggctcatgta aattgcagat ccaagttcct gcaaatggcg agtacgaaaa
     3721 acccgaacaa ctcgttggaa agaagattgt atcctccttt acaaagttga gtactgacta
     3781 tttcaagaaa ttatccgaca aacctacgaa tattagatac gttggtggtt cagttgaggc
     3841 ttcttgcgca ttgggtgttg ccgacgccat tgtcgattta gttgaaagtg gagagaccat
     3901 gaaagctgct ggattaaagg cgatcgaaac catattggaa acttccgctc atttgatttc
     3961 ctccaagaaa accaaatttc cagaaatggt caacataatt gtccaaagac ttcaaggtgt
     4021 tttagcggct caggaatatg tcttgtgtaa ctacaatgct ccaaaatcca ttcaatcaaa
     4081 gtgcttaacc attactccag gtagaagggc agccactgtc tccactttgg ataaacacag
     4141 tgacgacgaa gatgactggg ttgccatttc ttccatggtt aacagaaagg aaattggtaa
     4201 tgtaatggat gaattgaaga aagctggcgc aacagatata ttggtgctcg agatatcaaa
     4261 ttgtagagtt taataaaata ctatatatac atacttatag atttacttca atttgactca
     4321 atctgtctta taccatcttt atctaccacc ttgatgtaca cacctttaaa atcaatgggc
     4381 attctcgttt gtaattcttt gacacacatg tccattaact tcaaaccatc ttcaactgtc
     4441 atgtcctttc tataatgttt atctaacaag gatgttgtgt aaaatgctgc gtagccatga
     4501 gcaccataag gacgttcaac ttgcgtacct aaataatcta tccaattcaa cgacggctga
     4561 tttgtcttta catcgtatcc tccaattaaa cagttcacct ggtacggttt tctagaaggg
     4621 cgaattctgc agatatccat cacactggta taacttcgta tagcatacat tatacgaagt
     4681 tatggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4741 aggcctaact agatagcagt cagtcagtca ggataacgca ggaaagaaca tgtgagcaaa
     4801 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4861 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4921 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4981 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5041 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5101 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5161 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5221 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5281 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcgg
//
