LOCUS       V008242                 5253 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008242
VERSION     V008242
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5253)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5253)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(513..617)
                     /label="AmpR promoter"
     CDS             776..805
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             2576..3694
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(3947..3980)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(4146..4734)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(join(4908..5253,1..512))
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
       61 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      121 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      181 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      241 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      301 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      361 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      421 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      481 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      541 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
      601 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaactcgag
      661 ccgcggaggc ctgggcccca ggtcgactct agatcgcgag ctagcacgcg tggtacccgt
      721 tcccgggtta attaacggtg aacaaaagct aatctccgag gaatacttga acggtgaaca
      781 aaaattaatc tcagaagaag acttgaacgg actcgacggt gaacaaaagt tgatttctga
      841 agaagatttg aacggtgaac aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa
      901 gttaatctca gaagaagact tgaacggact cgacggtgaa caaaagttga tttctgaaga
      961 agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg aacaaaaatt
     1021 aatctcagaa gaagacttga acggactcga cggtgaacaa aagttgattt ctgaagaaga
     1081 tttgaacggt gaacaaaagc taatctccga ggaagacttg aacggtgaac aaaaattaat
     1141 ctcagaagaa gacttgaacg gactcgacgg tgaacaaaag ttgatttctg aagaagattt
     1201 gaacggtgaa caaaagctaa tctccgagga agacttgaac ggtgaacaaa aattaatcaa
     1261 tcacgcatgc gattatataa ctagatagac cgtgcatcgc taccattgtt caccacaaat
     1321 gtttctaaga gtgaaattct ggaaatctgg aaatctggtt ttgtattctt gttattcttc
     1381 tttttgttat tacatatata acttgttact tttttaaaaa aatctttgtt tattttataa
     1441 atatataaaa ctaaatttaa gaaaaagaga aaaatgtttt atttgagaga ttgaaatttt
     1501 acttgaattt agcttagctt ttataaagta ttattatgta aaaaaacaaa acaaatatac
     1561 attaaaaagt taagactata aaatagccac ccaaggcatt tctatatcta cagtctgcca
     1621 gacttgccac ctgacgtcta agaaaataac ttcgtatagc atacattata cgaagttata
     1681 gctcggatcc actagtaacg gccgccagtg tgctggaatt cgcccttgga tccaatcatc
     1741 actggtgggg gtcaagaaag aggtttgaga tcaggtactt tggcaccacc attagtagct
     1801 gggtttggtg aagctgcaag attaatgaaa caagaatctg catttgacaa gaaacatatt
     1861 gagaggttat ctactaaatt gaagaacggt ttgttatcca ttccatctac tcagtttaac
     1921 ggttgtaata acccaacata tcaataccca ggttgtgtga atgtttcctt tgcatacatt
     1981 gaaggggaat ctttgttaat ggcattgaaa gatattgcat tgagttctgg ttctgcatgt
     2041 acctctgcat ctttggaacc atcgtatgtt ttacatgctt tgggtgccga tgatgccttg
     2101 gctcattctt ccattagatt cggtattggt agattcacca ctgaagcaga agttgactat
     2161 gttattcaag caattaatga aagagttgat ttcttgagaa agatgtctcc attatgggaa
     2221 atggttcaag aaggtattga cttgaactct atcgaatgga gtggacatta aggttttttg
     2281 tttttttgta atttgtgttg ttatgtgtaa ttatcgtgta tagagtaatt tcggaaattg
     2341 tactttcgtt catgtatctg aaccagatga ataatacgtt cgtattgttg aagaatgact
     2401 aactcctgta acggcgtgcg tgggttccgc agccgggttc aatttttttt ttcttgttca
     2461 ctataaaaat atataataaa cgaaaaaaat ccgagggact caggctatga aatttttcac
     2521 tcaagaattt tttttccttc atccttaatt cctttattca ctacccaaac ccacaatgtc
     2581 agttaaaact aaaactatta caattttacc aggtgatcac gtcggtaccg aaattgtcaa
     2641 tgaagctatc aaagttttag aagcaattga agctgccact ccttaccaaa aaatccattt
     2701 cgatttcaaa catcatttaa tcggtggtgc tgccattgat gccactggtg tcccattgcc
     2761 agatgacgca cttgaaagtg ccaaaaaatc tgatgccgtc ttattgggtg ctgttggtgg
     2821 accaaaatgg gggactggcg ccgttcgtcc agaacaaggt ttattaaaga ttcgtaaaga
     2881 attgaacctt tatgctaaca tcagaccatg taactttgcc agtgactcct tattggaatt
     2941 atccccattg agaccagaag tcgttaaagg tacaaacttg atcattgttc gtgaattagt
     3001 tggtggtatt tatttcggtg atcgtgaaga acaagaagaa agtgaagata aacaaaccgc
     3061 atgggatact gaaaaataca ccgtcgatga agtcaccaga atcacccgta tggctgcctt
     3121 catggcttta caacataacc caccattacc aatctggtca ttggataagg ctaatgtttt
     3181 agcttcttct agattatgga gaagaactgt cgataaagtg atttctgaag agttcccaac
     3241 tttgtctgtt caacatcaat tgattgactc cgccgccatg attttaattc aaaacccaac
     3301 caaattgaat ggtataatca tcacttctaa catgtttggt gatatcattt ccgatgaagc
     3361 ttcagttatt ccaggttcct tgggtttatt accatctgca tctttggctt cattgccaga
     3421 taccaacacc gcttttggtc tttatgaacc atgtcacggt tctgctcctg atttaccagc
     3481 caataaagtt aacccaattg ctactatttt atctgctgct tctatgttga gattatcttt
     3541 ggattgtgtg aaagaagctg aagctttaga agaagccgtg aaacaagtct tggataaagg
     3601 tatcagaact gcagatttaa gaggtaccag ttcagctact gaagttggtg acgcgattgt
     3661 cgaagctgtt actaaaatct taaaagaaaa agcttaagta aagtatacac aaaatttaac
     3721 gaatattatt aatacaaaat gaacaccacc taaatacttt tttttaaaaa tgtaccgact
     3781 cttctaaaaa ttgcgataat tcaatatcgg aaatttctct caccggttta cttggatctt
     3841 cggaaataaa tctatttgaa gtaatggtag cctcacttga ttctgattgg ctctttctag
     3901 acatgggtag gtaagggcga attctgcaga tatccatcac actggtataa cttcgtatag
     3961 catacattat acgaagttat ggtaatacgg ttatccacag aatcagggga taacgcagga
     4021 aagaacatgt gagcaaaagg cctaactaga tagcagtcag tcagtcagga taacgcagga
     4081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4561 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4681 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5221 acaggcatcg tggtgtcacg ctcgtcgttt ggt
//
