LOCUS       40924_13020        5229 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPC50, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Fast and convenient doxycycline-induced marker recycling in Candida 
            albicans via Cre-loxP system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5229)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5229)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(578..1435)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1436..1540)
                     /label=AmpR promoter
     protein_bind    complement(4906..4939)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(5045..5229,1..404))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
       61 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      121 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      181 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      241 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      301 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      361 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      421 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      481 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      541 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      601 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      661 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
      721 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      781 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      841 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      901 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      961 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1021 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1081 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1141 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1201 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1261 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1321 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1381 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1441 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1501 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1561 cacctgacgt ctaagaaaat aacttcgtat agcatacatt atacgaagtt atagctcgga
     1621 tccactagta acggccgcca gtgtgctgga attcgccctt aagaacattt ctgtaccgca
     1681 aatgtatcga ctttttcgat ggtgtgttat tgttgttttg ttgcgaaata taatagcttc
     1741 tgctttatat cctttgtgtt gatccttccc ctcctttcat acttgtaaaa ttacaattgg
     1801 tattttcaac caatattacc acacgtcatg cactaactca aatgggcaaa aaaaagagag
     1861 ataggaatta cgtcatttta ttatgttttt acgtggacta gaaggttaag ataatttctg
     1921 gttattttgt tgataattga gttttgttcg acctccacca atctttaaac tctcgttgag
     1981 tattcatttt aatgattttt tgaaatcaga aggggtgttt ctaacccagg attgttttat
     2041 tgagaggttg aaacatcaat gtgttagttt attgatttgg gttggtttaa tagaaaaata
     2101 tcagtaggtg ttcaataatt gtagtttatt ttcttggcta aaattgttgc gaaaaattga
     2161 caaaataata acaaaaagta ttttagatgc taaaacataa tagtaggaat atctacctga
     2221 accatttaca ttcaacaact ggcaagaacc ttcctaataa tttgcaagag taatcacaat
     2281 taaaccaaaa tcaaagttta ccgtgtatat agttggacag aaagctgtca cattggttta
     2341 atacatagtt gaatatgtat gcatgaaaca acaacaatac aattacagct catgatcctt
     2401 caattaattt caacattcaa acaacataac accttgtgta aatgaggtta tgagtacatg
     2461 tgaacaacaa atcttatgac taaactcggg tcgtgtactt ttgacgattg agtcattttt
     2521 accaattagg caattaattt ttgctaaatt ttcattaacc gcgctggcca tcccaacaaa
     2581 caatataaaa gctgacaacg aaaaaaaaaa aaaaaaaaaa aaaactccaa ttctgaaaaa
     2641 tttttcttct tcttcatact aaacaaaaca actataaaga taatcaatta ataatgtcac
     2701 aacaacctag tgaaaataaa ttatggggtg gtcgattcac tggcgccact gatccattaa
     2761 tggatttata caatgcttca ttaccttatg ataaagttat gtatgatgca gatttaactg
     2821 gtactaaagt atatactcaa ggattgaaca aattaggatt aattaccact gaagaattga
     2881 atctgattca tcaaggttta gaacaaatta gacaagaatg gcatgataat aaatttatta
     2941 ttaaagctgg tgatgaagat attcatactg ctaatgaacg tagattagga gaaatcattg
     3001 ggaaaaacat ttctggtaaa gttcatactg gtagatccag aaatgatcaa gttgctactg
     3061 atatgagaat ttttgttcgt gaatcattac ttaatttaag taagatttta catcaattca
     3121 ttacagcaat tcttgaacga gctcataaag aaattgatgt attaatgcct ggttatactc
     3181 atttacaaaa agctcaacct attagatggg ctcattggtt aagttcatat gcaacttatt
     3241 tcactgaaga ttataaacgt ttacaagaaa tcattactcg tgttaatcaa tctccattag
     3301 gatctggtgc tttagctggt catccctacg gtattgatcg tgaattttta gctaaaggat
     3361 taggatttga tggtgttatt gggaattctt taactgcagt ttctgatcgt gattttgttg
     3421 ttgaaagttt attttggagt acattattta tgaatcatat ttctcgattt tcagaagatt
     3481 taattattta ttctagtggt gaatttggat ttataaagtt agctgatgct tactctactg
     3541 gttcatcatt aatgccacaa aagaaaaatc ctgattcatt agaattatta cgtgggaaaa
     3601 gtggcagagt gtttggtcag ttatctgggt ttttaatgtc aattaaatcc attccatcaa
     3661 cctataataa agatatgcaa gaagataaag aaccattatt tgatgcattg actaccgtag
     3721 aacattcaat tttaattgct actggtgtga tttctacttt actgattgat aaacaaaata
     3781 tggaaaaagc tttaactatg gatatgttag ctactgattt agcagattat ttagttagaa
     3841 aaggtgttcc atttagagaa actcatcata tttctggtga atgtgttaga aaagctgaag
     3901 aagaaaaatt gtcgggaatt gaccaattat catttgaaca atttcaacaa attgattcac
     3961 gttttgaaaa agatgttatg gaaacttttg attttgaagc tagtgtggaa agaagagatg
     4021 ctattggtgg tactgctaag agcgctgttt taaaacagtt agaaaatttg aaatcaattt
     4081 taaattaatt tataaataat catatagtat tcaatgtttt gttttgtttt tttttttttt
     4141 tttatggaaa tgtacaaaca gtatataatc aattaataga aagataccct gtattccatt
     4201 gtatcgcctt tgggatgtct ataataccaa ctttcaagaa tgagaaatta aacaacaatt
     4261 tctttcacca atcaactcgt ctatattcaa taaactcatt ctggtctttt caaatattta
     4321 atgttatcct gatcaaactt acaaatcaaa aatgtatcaa aatcattcat tagttcttct
     4381 tcttttgtta tctttgtcac tatatttctc tccatccaga ccaaaaatac ttccattgtg
     4441 aagatcgtct gaatcataat cagcaactac ctcttccatt tcagagaatt cagatttttc
     4501 aaaataggta tcacgggcca agtacacaca agacaaacga tacgagtgct gggatatttc
     4561 tttcaatgta tcactaaatt cgttgagatt taaagagagc cctttgactg ctttcattac
     4621 tttcctcaca tcatgagcat ccacattcaa atttcctgta gccctttatt cttagttttc
     4681 ttttcttatt cttttcttcg tacattgaaa tcacaaatgg tgatatcaaa cgaaggtcac
     4741 actgacttat gtctaatacc tccaatgatg atactggtaa cttaaccagg tctactcttt
     4801 tcaaacgatt tctcattagt gtcaatttac gtaggttctt caacctttca aacgatgcaa
     4861 atagcttttg taagggcgaa ttctgcagat atccatcaca ctggtataac ttcgtatagc
     4921 atacattata cgaagttatg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4981 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5041 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5101 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5161 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5221 gaagcgtgg
//
