LOCUS       V008246                 4284 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008246
VERSION     V008246
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Fast and convenient doxycycline-induced marker recycling in Candida
            albicans via Cre-loxP system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4284)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4284)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(338..1195)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1196..1300)
                     /label="AmpR promoter"
     CDS             2448..3341
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(3721..3754)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(3860..4284,1..164))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
       61 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      121 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      181 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      241 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      301 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      361 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      421 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
      481 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      541 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      601 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      661 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      721 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      781 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      841 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      901 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
      961 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1021 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1081 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1141 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1201 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1261 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1321 cacctgacgt ctaagaaaat aacttcgtat agcatacatt atacgaagtt atagctcgga
     1381 tccactagta acggccgcca gtgtgctgga attcgccctt gtttgatacc atggcaagca
     1441 tactagaatt attatacgag ctgggtgtga aatgtgccac cgtttatgca ttttcgatcg
     1501 aaaacttcaa gagactgcaa tatgaggtca aatggttgat ggaattagcc aaatccaagt
     1561 ttacgcaaat aaaccaacat ggattgttgt gtgaggagta tggggttaaa atcagaatat
     1621 taggaaatac caaacttttg ccgaaagatg tgttagagat ccttgaaaga accgaagaaa
     1681 taactaaaaa caataagcgt gctgtgttga atgtctgctt cccatataca tcaagagacg
     1741 agataaccca tctgatcaag tcagtagtag acgaagcgac gaaaggtggt attgaaataa
     1801 ccgaggaggc tatcgacaag catctttata cgaaagatgt gcccccattg gatttactag
     1861 taagaacatc aggaacattt agactttcag actttctttt atggcaaacg gtgtcgccag
     1921 attgtgcagt tgtgtttgtc gacaagttat ggccggcatt ctagaccatg ggatatgctc
     1981 aagattttac tcaactgggg gtttaacaaa tacatgtatg gaaacccaaa tggatatggg
     2041 gttagtaata acttggtagt gaagagccaa atggaagaag aattaataaa cacaccaaat
     2101 ggagctacag ggcttgaccg gtacctggag gacgaagaga ccgaagttag tagtaacaat
     2161 ttaggaactg aagaggatac agtgacatcc gaagaagaag atgttgaaga tataggcaaa
     2221 aagtaatagg acagtatttt atttattcaa tagttttttt tttttttttg aaacgtacac
     2281 cttgtttatt tcaggtgttt ctttgaaacg tgtctcaaag cgataacggg tacaaaaaaa
     2341 aatgtattga gtcattgaaa tttttttatt tttttttttt tttggtgaag atttttccca
     2401 caccaaatat ataccacttt tcttctaaca ccacctacat attcactatg gatttagtca
     2461 atcatttgcc cgatcgtttg ttatttgctg ttcccaaaaa gggcagatta tatgaaaaat
     2521 gctgcagctt attgagcggt gccgatatac agtttagaag atctaataga ttggatattg
     2581 ccctttccac aaacttgcca attgcattaa tctttttgcc tgcagccgat attccagttt
     2641 tcgttggaga aggtaattgt gacttgggta taactgggtt agaccagatc aaagaagcag
     2701 accaattcga caacattgag gatttgttgg atttgaagtt tggctcatgt aaattgcaga
     2761 tccaagttcc tgcaaatggc gagtacgaaa aacccgaaca actcgttgga aagaagattg
     2821 tatcctcctt tacaaagttg agtactgact atttcaagaa attatccgac aaacctacga
     2881 atattagata cgttggtggt tcagttgagg cttcttgcgc attgggtgtt gccgacgcca
     2941 ttgtcgattt agttgaaagt ggagagacca tgaaagctgc tggattaaag gcgatcgaaa
     3001 ccatattgga aacttccgct catttgattt cctccaagaa aaccaaattt ccagaaatgg
     3061 tcaacataat tgtccaaaga cttcaaggtg ttttagcggc tcaggaatat gtcttgtgta
     3121 actacaatgc tccaaaatcc attcaatcaa agtgcttaac cattactcca ggtagaaggg
     3181 cagccactgt ctccactttg gataaacaca gtgacgacga agatgactgg gttgccattt
     3241 cttccatggt taacagaaag gaaattggta atgtaatgga tgaattgaag aaagctggcg
     3301 caacagatat attggtgctc gagatatcaa attgtagagt ttaataaaat actatatata
     3361 catacttata gatttacttc aatttgactc aatctgtctt ataccatctt tatctaccac
     3421 cttgatgtac acacctttaa aatcaatggg cattctcgtt tgtaattctt tgacacacat
     3481 gtccattaac ttcaaaccat cttcaactgt catgtccttt ctataatgtt tatctaacaa
     3541 ggatgttgtg taaaatgctg cgtagccatg agcaccataa ggacgttcaa cttgcgtacc
     3601 taaataatct atccaattca acgacggctg atttgtcttt acatcgtatc ctccaattaa
     3661 acagttcacc tggtacggtt ttctagaagg gcgaattctg cagatatcca tcacactggt
     3721 ataacttcgt atagcataca ttatacgaag ttatggtaat acggttatcc acagaatcag
     3781 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3841 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3901 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3961 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4021 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4081 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4141 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4201 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4261 agttcttgaa gtggtggcct aact
//
