LOCUS       40924_13005        6709 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC47, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6709)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             19..360
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      378..684
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     CDS             1031..2437
                     /codon_start=1
                     /product="Arg4"
                     /label=Arg4
                     /protein_id="AVR52641.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
     protein_bind    2793..3063
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     rep_origin      complement(3559..4147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4321..5178)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5179..5283)
                     /label=AmpR promoter
     regulatory      5354..6088
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             6089..6706
                     /label=TetR
                     /note="tetracycline repressor TetR"
ORIGIN
        1 tcgactttgt ttggtggcgc caattttaat caaagtggga atattgctga tagctcattg
       61 tccttcactt tcactaacag tagcaacggt ccgaacctca taacaactca aacaaattct
      121 caagcgcttt cacaaccaat tgcctcctct aacgttcatg ataacttcat gaataatgaa
      181 atcacggcta gtaaaattga tgatggtaat aattcaaaac cattgtcacc tggttggacg
      241 gaccaaactg cgtataacgc gtttggaatc actacaggga tgtttaatac cactacaatg
      301 gatgatgtat ataactatct attcgatgat gaagataccc caccaaaccc aaaaaaagag
      361 taaaccgtgc atcgctacca ttgttcacca caaatgtttc taagagtgaa attctggaaa
      421 tctggaaatc tggttttgta ttcttgttat tcttcttttt gttattacat atataacttg
      481 ttactttttt aaaaaaatct ttgtttattt tataaatata taaaactaaa tttaagaaaa
      541 agagaaaaat gttttatttg agagattgaa attttacttg aatttagctt agcttttata
      601 aagtattatt atgtaaaaaa acaaaacaaa tatacattaa aaagttaaga ctataaaata
      661 gccacccaag gcatttctat atctacagtc tgccagactc aatttatgac atattgaccg
      721 acataattca gtcatttcta atattcaaat aacaagacac cttgtataaa tgaatccaca
      781 atcgtatatg aacaaccaaa ttttatgact aactttgggt cgtgtacttt tgacgattga
      841 gtcattttta ccaattaggt aattgaattt tgttaatttg tcattaaccg cgctggccat
      901 cccaacaata acaacaatca atttaaaaac ttgacaaggc aaaaaaaaaa aaattttatc
      961 tttgaaaaat ttttcttcct tttcttcttc tggtctttac ataacagcta tcaagaataa
     1021 tcaattaata atgtcacaac aacaagataa acaacctagt gaaaataaat tatggggtgg
     1081 tcgtttcacc ggtgccactg atccattgat ggatttatat aatgcttcat taccttatga
     1141 taaagttatg tatgatgctg atttaactgg tactaaagta tacacacaag gattaaacaa
     1201 attaggatta attaccactg aagaattgca tttgattcat caaggattag aacaaatcag
     1261 acaagaatgg catgataata aattcattat taaggcaggt gatgaggata ttcatactgc
     1321 taatgaacgt cgattaggag aaatcattgg gaaaaacatt tctggtaaag ttcacactgg
     1381 tagatcaaga aatgatcaag ttgctaccga tatgagaatt ttcgttcgtg aatcattact
     1441 taatttaagt aaaattttac atcaattcat cacggcaatt cttgaacggg cacataaaga
     1501 aatcgatgta ttaatgcctg gttatactca tttacaaaaa gctcaaccta ttagatgggc
     1561 acattggtta agttcatatg caacttattt caccgaagat tataaacgtt tacaagaaat
     1621 tataactcgt gttaaccaat ctccattagg atctggtgct ttagcaggtc atccttatgg
     1681 tattgatcgt gaatttttag ctaaaggatt aggatttgat ggtgtcattg ggaattcttt
     1741 aactgcagtt tcagatcgtg attttgttgt tgaaagttta ttttggagta cattatttat
     1801 gaatcatatt tctcgattct cagaagatct aattatttat tctagtggtg aatttgggtt
     1861 cataaaatta gctgatgctt attctactgg ttcttcattg atgccacaaa agaaaaaccc
     1921 tgactcatta gaattattac gtgggaaaag tggtagagta tttggtcaat tatctggatt
     1981 tttaatgtcg atcaaatcta ttccatctac ttataataaa gatatgcaag aggataaaga
     2041 accattattt gatgcattga ctacagtgga acattcaatt ttaattgcta ctggtgtgat
     2101 ttctacttta ctgattgata aacaaaatat ggaaaaagct ttaactatgg atatgttggc
     2161 tactgattta gctgattatt tagttagaaa aggagttcca tttagagaaa ctcatcatat
     2221 atctggtgaa tgtgttagaa aagctgaaga agaaaaatta tctggaattg atcaattatc
     2281 ttttgaacaa tttcaacaaa ttgattctcg ttttgaaaaa gacgttatgg aaacttttga
     2341 ttttgaagct agtgtggaaa gaagagatgc tattggtggt actgctaaaa gtgccgtttt
     2401 aaaacaatta gaaaatttaa aatcaatttt aagttaattt ataaatagtc atataataat
     2461 cacagtattg tttaacttac ataaaatata atcaattaac acagagatac cttgtactcc
     2521 aatactagtg ttataatgtt tataacagca gctttcaaga ataaggaact aaataaaagc
     2581 ttcttccatc acaacacaca acacgtctat atgccataaa tacataacgg tcttcctaat
     2641 aatatcctaa ccatacttgc aaatgcagtc agtcagtcag tcaggcttgg acagcatcag
     2701 cagtagcgta ttgttcgaat gaaccatcgt gagtgtaacc agacaagtca gcttcaccac
     2761 agtttggttc agcaccttgt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2821 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2881 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2941 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     3001 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     3061 agaaaagtga aagtcgagct ccaaacttct tttttatttt tcgcaatatt gtcgataaat
     3121 taaatataaa ctactcgatt gagtaataga ttcaataact atgggaatta ctaacaatga
     3181 tttaaggaaa aagcttcact taaaaataaa gccctttcaa ttaaagtgtt gtccatgttt
     3241 gtcgtttctg atgggctttt ctgaaatata aatatagatt caaatctctt gtattcaagt
     3301 tttagatttt tttttatttc ttttatatca ttatcaaatc aaatcaacaa actctttcaa
     3361 cttctcagaa agaaatcaat atcaactaca ataaaaccaa ctacgtctac tactacgtta
     3421 actcctacac acatacaaat ataaataggt aatcatggtc ataccgcgga ggcctgggcc
     3481 cgataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3961 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4141 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4261 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4321 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4381 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4441 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4501 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4561 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4621 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4681 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4741 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4801 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4861 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4921 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4981 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5041 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5101 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5161 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5221 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5281 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa ccgcggaggc ctgggccccc
     5341 gggtaccgag ctcgccagcg agaaagagga gtattggcat tgttgggtag tagatgaatg
     5401 agtaatcttc tagccaaaac aaaataaacc acaagaacag taggcaaaac tgagtacttg
     5461 atagcgcttg tagtctttgt atttaaaaaa acctaattgt ccaaaaattg aaaatcatga
     5521 gttgcaaatt tgttgaaatc accaggtaaa tagatcattt agctatatcg ccttatcatt
     5581 cttcgtacaa gaattataca caaataccac caccacacca cccagaaatt ttacacgtgt
     5641 tttctaaatt attactttcc gtggccaatc aaattgaaat ttttcttgtt cgaaacggca
     5701 gtgcctcaaa cattatagta gcaagtattg cctaaagagt ttccgtctcg tgcccccccc
     5761 ctatcccctt ttgtaatttg ccaaaatagc tcaaaaagga taatcccgca cttttttttt
     5821 ccgagttctc gtcatgcaag caagcttatt cagaattttc agaggtgcga gtctcaccac
     5881 taccactgca gctgcatctt ttacagcaac agcaacagca ggtgccacca cggcaaagac
     5941 attgtctgga tccactgtgc tccgaaaact gtataaaagg acctattcat ccctggtctt
     6001 atcttctcca gaattatttt tttttcatca gtttaacaac aacaaacgtt attgtcatac
     6061 aacaacaaca acaaatacaa aaacaattat gtcaagatta gataaatcaa aagttattaa
     6121 ttcagcttta gaattattaa atggtgttgg tattgaaggt ttaactacta gaaaattagc
     6181 tcaaaaatta ggtgttgaac aaccaacttt atattggcat gttaaaaata aaagagcttt
     6241 attagatgct ttaccaattg aaatgttaga tagacatcat actcattcat gtccattaga
     6301 aggtgaatca tggcaagatt ttttaagaaa taatgctaaa tcatatagat gtgctttatt
     6361 atcacataga gatggtgcta aagttcattt aggtactaga ccaactgaaa aacaatatga
     6421 aactttagaa aatcaattag cttttttatg tcaacaaggt ttttcattag aaaatgcttt
     6481 atatgcttta tcagctgttg gtcattttac tttaggttgt gttttagaag aacaagaaca
     6541 tcaagttgct aaagaagaaa gagaaactcc aactactgat tcaatgccac cattattaaa
     6601 acaagctatt gaattatttg atagacaagg tgctgaacca gcttttttat ttggtttaga
     6661 attaattatt tgtggtttag aaaaacaatt aaaatgtgaa tcaggtggt
//
