LOCUS       V008249                 6334 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008249
VERSION     V008249
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6334)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6334)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             726..1619
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    1998..2268
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     rep_origin      complement(2764..3352)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3526..4383)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4384..4488)
                     /label="AmpR promoter"
     regulatory      4559..5293
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             5294..5911
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             5933..6274
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
ORIGIN
        1 tctggaaatc tggttttgta ttcttgttat tcttcttttt gttattacat atataacttg
       61 ttactttttt aaaaaaatct ttgtttattt tataaatata taaaactaaa tttaagaaaa
      121 agagaaaaat gttttatttg agagattgaa attttacttg aatttagctt agcttttata
      181 aagtattatt atgtaaaaaa acaaaacaaa tatacattaa aaagttaaga ctataaaata
      241 gccacccaag gcatttctat atctacagtc tgccagactt gtatggtaat ccaaatgggt
      301 atggggttag tagtaatttg gtagtgaaaa ccccggtaga cggagaatta ataaataccc
      361 caaaatccac cacaggactc gacaggtacc tggaggatga ggagacagaa gttagtagta
      421 acaatttggg gacagaagag gatacagtga catcagagga agaagacagc gaaggtatag
      481 tcaaaaagca ataggacagc aagacaattt atttatttaa tagtttttta taaatgtaaa
      541 atctatcaca acatgtgtac ttaattttct gtggtagggg aggccatttc gttgaaacgt
      601 gtcgcgagcg gttgtgagaa ttgactcatt ttgattatta cgcaaccatt gaaatttttt
      661 attttttttt ggtgaagatt tttcccacac aacttcttct tttacttaac caccaactac
      721 cgataatgga tttagtcaat catttaccag accgtttgtt atttgctgtt cctaaaaagg
      781 gcagattata cgaaaaatgc tgtaacttat tgagtggtgc cgatatacag tttagaagat
      841 ctaatagatt agatatagca ctttctacaa acttgccaat tgcattaatc ttcttgcctg
      901 cagctgatat cccagttttc gttggagaag gtaattgtga cttgggtata actgggttag
      961 accaaatcaa agaagctgaa caattcgaca acatcgagga cttgttggat ttgaaatttg
     1021 gttcatgcaa attgcagatc caagttccag cagatggcga gtacgaaaag ccagaacagc
     1081 ttgttggaaa gaaaattgtg tcttcattta caaaattgag taccgactat ttcaaacaat
     1141 tgtcagacaa acctactaat atcagatatg tcggtggttc cgttgaggct tcttgtgcct
     1201 tgggtgttgc tgatgctatt gtcgatttgg ttgaaagtgg tgaaactatg aaagcagctg
     1261 ggttaaaggc catagaaacc atattggaga cttcggctca tttgatttcg tcaaagaaaa
     1321 gtaaattccc agaaatggtc aatataatcg tccaaagact tcaaggtgtt ttagctgccc
     1381 aggaatatgt cttgtgtaac tacaatgccc caaaatccat ccaagcaaaa tgtttaacca
     1441 ttactccagg cagaagggcc gctactgtct ctactttgga caaacacagc gacgacgaag
     1501 aggactgggt tgccatctca tccatggtta atagaaagga aatcggtaat gtaatggacg
     1561 aattgaagaa agctggtgca accgatatat tggtactcga gatatcaaat tgtagagttt
     1621 aaaagaagtg atagtttctc ataaatatta tacatatata cttatagatc tacttcaatt
     1681 tgactcaatc tgtcttatac catctttatc taccaccttg atgtacacac ctttaaagtc
     1741 aataggcatt ctcgtttgta attccttgac acacatatcc attaacttta aaccatcttc
     1801 gaccgtcatg tcctttctat aatgtttatc taacaaagat gttgtgtaaa atgcagcgta
     1861 gccatgagca ccatcagtca gtcagtcagg cttggacagc atcagcagta gcgtattgtt
     1921 cgaatgaacc atcgtgagtg taaccagaca agtcagcttc accacagttt ggttcagcac
     1981 cttgtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2041 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2101 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2161 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2221 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2281 gagctccaaa cttctttttt atttttcgca atattgtcga taaattaaat ataaactact
     2341 cgattgagta atagattcaa taactatggg aattactaac aatgatttaa ggaaaaagct
     2401 tcacttaaaa ataaagccct ttcaattaaa gtgttgtcca tgtttgtcgt ttctgatggg
     2461 cttttctgaa atataaatat agattcaaat ctcttgtatt caagttttag attttttttt
     2521 atttctttta tatcattatc aaatcaaatc aacaaactct ttcaacttct cagaaagaaa
     2581 tcaatatcaa ctacaataaa accaactacg tctactacta cgttaactcc tacacacata
     2641 caaatataaa taggtaatca tggtcatacc gcggaggcct gggcccgata acgcaggaaa
     2701 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2761 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2821 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2881 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2941 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3001 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3061 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3121 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3181 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3241 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3301 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3361 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3421 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3481 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3541 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3601 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3661 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3721 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3781 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3841 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3901 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3961 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4021 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4081 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4141 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4201 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4261 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4321 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4381 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4441 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4501 aaaagtgcca cctgacgtct aagaaccgcg gaggcctggg cccccgggta ccgagctcgc
     4561 cagcgagaaa gaggagtatt ggcattgttg ggtagtagat gaatgagtaa tcttctagcc
     4621 aaaacaaaat aaaccacaag aacagtaggc aaaactgagt acttgatagc gcttgtagtc
     4681 tttgtattta aaaaaaccta attgtccaaa aattgaaaat catgagttgc aaatttgttg
     4741 aaatcaccag gtaaatagat catttagcta tatcgcctta tcattcttcg tacaagaatt
     4801 atacacaaat accaccacca caccacccag aaattttaca cgtgttttct aaattattac
     4861 tttccgtggc caatcaaatt gaaatttttc ttgttcgaaa cggcagtgcc tcaaacatta
     4921 tagtagcaag tattgcctaa agagtttccg tctcgtgccc cccccctatc cccttttgta
     4981 atttgccaaa atagctcaaa aaggataatc ccgcactttt tttttccgag ttctcgtcat
     5041 gcaagcaagc ttattcagaa ttttcagagg tgcgagtctc accactacca ctgcagctgc
     5101 atcttttaca gcaacagcaa cagcaggtgc caccacggca aagacattgt ctggatccac
     5161 tgtgctccga aaactgtata aaaggaccta ttcatccctg gtcttatctt ctccagaatt
     5221 attttttttt catcagttta acaacaacaa acgttattgt catacaacaa caacaacaaa
     5281 tacaaaaaca attatgtcaa gattagataa atcaaaagtt attaattcag ctttagaatt
     5341 attaaatggt gttggtattg aaggtttaac tactagaaaa ttagctcaaa aattaggtgt
     5401 tgaacaacca actttatatt ggcatgttaa aaataaaaga gctttattag atgctttacc
     5461 aattgaaatg ttagatagac atcatactca ttcatgtcca ttagaaggtg aatcatggca
     5521 agatttttta agaaataatg ctaaatcata tagatgtgct ttattatcac atagagatgg
     5581 tgctaaagtt catttaggta ctagaccaac tgaaaaacaa tatgaaactt tagaaaatca
     5641 attagctttt ttatgtcaac aaggtttttc attagaaaat gctttatatg ctttatcagc
     5701 tgttggtcat tttactttag gttgtgtttt agaagaacaa gaacatcaag ttgctaaaga
     5761 agaaagagaa actccaacta ctgattcaat gccaccatta ttaaaacaag ctattgaatt
     5821 atttgataga caaggtgctg aaccagcttt tttatttggt ttagaattaa ttatttgtgg
     5881 tttagaaaaa caattaaaat gtgaatcagg tggttcgact ttgtttggtg gcgccaattt
     5941 taatcaaagt gggaatattg ctgatagctc attgtccttc actttcacta acagtagcaa
     6001 cggtccgaac ctcataacaa ctcaaacaaa ttctcaagcg ctttcacaac caattgcctc
     6061 ctctaacgtt catgataact tcatgaataa tgaaatcacg gctagtaaaa ttgatgatgg
     6121 taataattca aaaccattgt cacctggttg gacggaccaa actgcgtata acgcgtttgg
     6181 aatcactaca gggatgttta ataccactac aatggatgat gtatataact atctattcga
     6241 tgatgaagat accccaccaa acccaaaaaa agagtaaacc gtgcatcgct accattgttc
     6301 accacaaatg tttctaagag tgaaattctg gaaa
//
