LOCUS       V008250                 6318 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008250
VERSION     V008250
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6318)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6318)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             725..1534
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    1916..2186
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     rep_origin      complement(2682..3270)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3444..4301)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4302..4406)
                     /label="AmpR promoter"
     regulatory      4483..5217
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             5218..5835
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             5857..6198
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
ORIGIN
        1 ttactttttt aaaaaaatct ttgtttattt tataaatata taaaactaaa tttaagaaaa
       61 agagaaaaat gttttatttg agagattgaa attttacttg aatttagctt agcttttata
      121 aagtattatt atgtaaaaaa acaaaacaaa tatacattaa aaagttaaga ctataaaata
      181 gccacccaag gcatttctat atctacagtc tgccagactc aaaaccggat cttcagaaca
      241 aaatccattt caaaaaaaac ttatgaatta tttaaacagt ttgaactgag agtaattaca
      301 aagtactaat aggaattgat ttggatggta taaacggaaa caaaaaaaag agctggtact
      361 actttcttta aaattatttt attatttgat tttatttaat agtatatatt atattttgaa
      421 cgtagattat tttgttgaaa gttgctgtag tgccattgat tcgtaacact aattctgtat
      481 tagtcattcc tcttgtttga tagtatccaa aaaaacggct atttttttgc aatcttattt
      541 cctgcatatt atacagataa cataatgaaa gaaaaaatct ttttttttgt tcttcaatga
      601 tgatttcaac cattctttta aacattgatc aattcctgag caacaacccc atacacactg
      661 gtttatatat cgcccctttt acagttgaag aaagaaatag aaatagaaat agcaaacaaa
      721 agatatgaca gtcaacacta agacctatag tgagagagca gaaactcatg cctcaccagt
      781 agcacaacga ttatttcgat taatggaact gaagaaaacc aatttatgtg catcaattga
      841 tgttgatacc actaaggaat tccttgaatt aattgataaa ttgggtcctt atgtatgctt
      901 aatcaagact catattgata taatcaatga tttttcctat gaatccacta ttgaaccatt
      961 attagaactt tcacgtaaac atcaatttat gatttttgaa gatagaaaat ttgctgatat
     1021 tggtaatacc gtgaagaaac aatatattgg tggagtttat aaaattagta gttgggcaga
     1081 tattactaat gctcatggtg tcactgggaa tggagtagtt gaaggattaa aacagggagc
     1141 taaagaaacc accaccaacc aagagccaag agggttattg atgttagctg aattatcatc
     1201 agtgggatca ttagcatatg gagaatattc tcaaaaaact gttgaaattg ctaaatccga
     1261 taaggaattt gttattggat ttattgccca acgtgatatg ggtggacaag aagaaggatt
     1321 tgattggctt attatgacac ctggagttgg attagatgat aaaggtgatg gattaggaca
     1381 acaatataga actgttgatg aagttgttag cactggaact gatattatca ttgttggtag
     1441 aggattgttt ggtaaaggaa gagatccaga tattgaaggt aaaaggtata gagatgctgg
     1501 ttggaatgct tatttgaaaa aaactggcca attataaatg tgaaggggga gattttcact
     1561 ttattagatt tgtatatatg tataataaat aaataaataa gttaaataaa taattaaata
     1621 agggtggtaa ttattactat ttacaatcaa aggtggtcct tctagaaatc atgacagggg
     1681 ggtcaaatgt tttacgcttc aagaacatca ttataatctt caaaattatc acttaattta
     1741 gttctcatta tttcaaatac taataaggca atcgaagtag ctgggatagt agcagtcagt
     1801 cagtcaggct tggacagcat cagcagtagc gtattgttcg aatgaaccat cgtgagtgta
     1861 accagacaag tcagcttcac cacagtttgg ttcagcacct tgtcgagttt accactccct
     1921 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     1981 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2041 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2101 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     2161 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctccaaact tcttttttat
     2221 ttttcgcaat attgtcgata aattaaatat aaactactcg attgagtaat agattcaata
     2281 actatgggaa ttactaacaa tgatttaagg aaaaagcttc acttaaaaat aaagcccttt
     2341 caattaaagt gttgtccatg tttgtcgttt ctgatgggct tttctgaaat ataaatatag
     2401 attcaaatct cttgtattca agttttagat ttttttttat ttcttttata tcattatcaa
     2461 atcaaatcaa caaactcttt caacttctca gaaagaaatc aatatcaact acaataaaac
     2521 caactacgtc tactactacg ttaactccta cacacataca aatataaata ggtaatcatg
     2581 gtcataccgc ggaggcctgg gcccgataac gcaggaaaga acatgtgagc aaaaggccag
     2641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     2881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3121 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     4441 gaacccggga agcttggatc ccttaagaca gtctgccaga ctgccagcga gaaagaggag
     4501 tattggcatt gttgggtagt agatgaatga gtaatcttct agccaaaaca aaataaacca
     4561 caagaacagt aggcaaaact gagtacttga tagcgcttgt agtctttgta tttaaaaaaa
     4621 cctaattgtc caaaaattga aaatcatgag ttgcaaattt gttgaaatca ccaggtaaat
     4681 agatcattta gctatatcgc cttatcattc ttcgtacaag aattatacac aaataccacc
     4741 accacaccac ccagaaattt tacacgtgtt ttctaaatta ttactttccg tggccaatca
     4801 aattgaaatt tttcttgttc gaaacggcag tgcctcaaac attatagtag caagtattgc
     4861 ctaaagagtt tccgtctcgt gccccccccc tatccccttt tgtaatttgc caaaatagct
     4921 caaaaaggat aatcccgcac tttttttttc cgagttctcg tcatgcaagc aagcttattc
     4981 agaattttca gaggtgcgag tctcaccact accactgcag ctgcatcttt tacagcaaca
     5041 gcaacagcag gtgccaccac ggcaaagaca ttgtctggat ccactgtgct ccgaaaactg
     5101 tataaaagga cctattcatc cctggtctta tcttctccag aattattttt ttttcatcag
     5161 tttaacaaca acaaacgtta ttgtcataca acaacaacaa caaatacaaa aacaattatg
     5221 tcaagattag ataaatcaaa agttattaat tcagctttag aattattaaa tggtgttggt
     5281 attgaaggtt taactactag aaaattagct caaaaattag gtgttgaaca accaacttta
     5341 tattggcatg ttaaaaataa aagagcttta ttagatgctt taccaattga aatgttagat
     5401 agacatcata ctcattcatg tccattagaa ggtgaatcat ggcaagattt tttaagaaat
     5461 aatgctaaat catatagatg tgctttatta tcacatagag atggtgctaa agttcattta
     5521 ggtactagac caactgaaaa acaatatgaa actttagaaa atcaattagc ttttttatgt
     5581 caacaaggtt tttcattaga aaatgcttta tatgctttat cagctgttgg tcattttact
     5641 ttaggttgtg ttttagaaga acaagaacat caagttgcta aagaagaaag agaaactcca
     5701 actactgatt caatgccacc attattaaaa caagctattg aattatttga tagacaaggt
     5761 gctgaaccag cttttttatt tggtttagaa ttaattattt gtggtttaga aaaacaatta
     5821 aaatgtgaat caggtggttc gactttgttt ggtggcgcca attttaatca aagtgggaat
     5881 attgctgata gctcattgtc cttcactttc actaacagta gcaacggtcc gaacctcata
     5941 acaactcaaa caaattctca agcgctttca caaccaattg cctcctctaa cgttcatgat
     6001 aacttcatga ataatgaaat cacggctagt aaaattgatg atggtaataa ttcaaaacca
     6061 ttgtcacctg gttggacgga ccaaactgcg tataacgcgt ttggaatcac tacagggatg
     6121 tttaatacca ctacaatgga tgatgtatat aactatctat tcgatgatga agatacccca
     6181 ccaaacccaa aaaaagagta aaccgtgcat cgctaccatt gttcaccaca aatgtttcta
     6241 agagtgaaat tctggaaatc tggaaatctg gttttgtatt cttgttattc ttctttttgt
     6301 tattacatat ataacttg
//
