LOCUS       40924_12990        6709 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC44, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6709)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             491..1897
                     /codon_start=1
                     /product="Arg4"
                     /label=Arg4
                     /protein_id="AVR52635.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
     protein_bind    2253..2523
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     rep_origin      complement(3019..3607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3781..4638)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4639..4743)
                     /label=AmpR promoter
     regulatory      4814..5548
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             5549..6166
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             6188..6529
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
ORIGIN
        1 agagaaaaat gttttatttg agagattgaa attttacttg aatttagctt agcttttata
       61 aagtattatt atgtaaaaaa acaaaacaaa tatacattaa aaagttaaga ctataaaata
      121 gccacccaag gcatttctat atctacagtc tgccagactc aatttatgac atattgaccg
      181 acataattca gtcatttcta atattcaaat aacaagacac cttgtataaa tgaatccaca
      241 atcgtatatg aacaaccaaa ttttatgact aactttgggt cgtgtacttt tgacgattga
      301 gtcattttta ccaattaggt aattgaattt tgttaatttg tcattaaccg cgctggccat
      361 cccaacaata acaacaatca atttaaaaac ttgacaaggc aaaaaaaaaa aaattttatc
      421 tttgaaaaat ttttcttcct tttcttcttc tggtctttac ataacagcta tcaagaataa
      481 tcaattaata atgtcacaac aacaagataa acaacctagt gaaaataaat tatggggtgg
      541 tcgtttcacc ggtgccactg atccattgat ggatttatat aatgcttcat taccttatga
      601 taaagttatg tatgatgctg atttaactgg tactaaagta tacacacaag gattaaacaa
      661 attaggatta attaccactg aagaattgca tttgattcat caaggattag aacaaatcag
      721 acaagaatgg catgataata aattcattat taaggcaggt gatgaggata ttcatactgc
      781 taatgaacgt cgattaggag aaatcattgg gaaaaacatt tctggtaaag ttcacactgg
      841 tagatcaaga aatgatcaag ttgctaccga tatgagaatt ttcgttcgtg aatcattact
      901 taatttaagt aaaattttac atcaattcat cacggcaatt cttgaacggg cacataaaga
      961 aatcgatgta ttaatgcctg gttatactca tttacaaaaa gctcaaccta ttagatgggc
     1021 acattggtta agttcatatg caacttattt caccgaagat tataaacgtt tacaagaaat
     1081 tataactcgt gttaaccaat ctccattagg atctggtgct ttagcaggtc atccttatgg
     1141 tattgatcgt gaatttttag ctaaaggatt aggatttgat ggtgtcattg ggaattcttt
     1201 aactgcagtt tcagatcgtg attttgttgt tgaaagttta ttttggagta cattatttat
     1261 gaatcatatt tctcgattct cagaagatct aattatttat tctagtggtg aatttgggtt
     1321 cataaaatta gctgatgctt attctactgg ttcttcattg atgccacaaa agaaaaaccc
     1381 tgactcatta gaattattac gtgggaaaag tggtagagta tttggtcaat tatctggatt
     1441 tttaatgtcg atcaaatcta ttccatctac ttataataaa gatatgcaag aggataaaga
     1501 accattattt gatgcattga ctacagtgga acattcaatt ttaattgcta ctggtgtgat
     1561 ttctacttta ctgattgata aacaaaatat ggaaaaagct ttaactatgg atatgttggc
     1621 tactgattta gctgattatt tagttagaaa aggagttcca tttagagaaa ctcatcatat
     1681 atctggtgaa tgtgttagaa aagctgaaga agaaaaatta tctggaattg atcaattatc
     1741 ttttgaacaa tttcaacaaa ttgattctcg ttttgaaaaa gacgttatgg aaacttttga
     1801 ttttgaagct agtgtggaaa gaagagatgc tattggtggt actgctaaaa gtgccgtttt
     1861 aaaacaatta gaaaatttaa aatcaatttt aagttaattt ataaatagtc atataataat
     1921 cacagtattg tttaacttac ataaaatata atcaattaac acagagatac cttgtactcc
     1981 aatactagtg ttataatgtt tataacagca gctttcaaga ataaggaact aaataaaagc
     2041 ttcttccatc acaacacaca acacgtctat atgccataaa tacataacgg tcttcctaat
     2101 aatatcctaa ccatacttgc aaatgcagtc agtcagtcag tcaggcttgg acagcatcag
     2161 cagtagcgta ttgttcgaat gaaccatcgt gagtgtaacc agacaagtca gcttcaccac
     2221 agtttggttc agcaccttgt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2281 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2341 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2401 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2461 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2521 agaaaagtga aagtcgagct ccaaacttct tttttatttt tcgcaatatt gtcgataaat
     2581 taaatataaa ctactcgatt gagtaataga ttcaataact atgggaatta ctaacaatga
     2641 tttaaggaaa aagcttcact taaaaataaa gccctttcaa ttaaagtgtt gtccatgttt
     2701 gtcgtttctg atgggctttt ctgaaatata aatatagatt caaatctctt gtattcaagt
     2761 tttagatttt tttttatttc ttttatatca ttatcaaatc aaatcaacaa actctttcaa
     2821 cttctcagaa agaaatcaat atcaactaca ataaaaccaa ctacgtctac tactacgtta
     2881 actcctacac acatacaaat ataaataggt aatcatggtc ataccgcgga ggcctgggcc
     2941 cgataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3001 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3061 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3181 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3421 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4681 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4741 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa ccgcggaggc ctgggccccc
     4801 gggtaccgag ctcgccagcg agaaagagga gtattggcat tgttgggtag tagatgaatg
     4861 agtaatcttc tagccaaaac aaaataaacc acaagaacag taggcaaaac tgagtacttg
     4921 atagcgcttg tagtctttgt atttaaaaaa acctaattgt ccaaaaattg aaaatcatga
     4981 gttgcaaatt tgttgaaatc accaggtaaa tagatcattt agctatatcg ccttatcatt
     5041 cttcgtacaa gaattataca caaataccac caccacacca cccagaaatt ttacacgtgt
     5101 tttctaaatt attactttcc gtggccaatc aaattgaaat ttttcttgtt cgaaacggca
     5161 gtgcctcaaa cattatagta gcaagtattg cctaaagagt ttccgtctcg tgcccccccc
     5221 ctatcccctt ttgtaatttg ccaaaatagc tcaaaaagga taatcccgca cttttttttt
     5281 ccgagttctc gtcatgcaag caagcttatt cagaattttc agaggtgcga gtctcaccac
     5341 taccactgca gctgcatctt ttacagcaac agcaacagca ggtgccacca cggcaaagac
     5401 attgtctgga tccactgtgc tccgaaaact gtataaaagg acctattcat ccctggtctt
     5461 atcttctcca gaattatttt tttttcatca gtttaacaac aacaaacgtt attgtcatac
     5521 aacaacaaca acaaatacaa aaacaattat gtcaagatta gataaatcaa aagttattaa
     5581 ttcagcttta gaattattaa atgaagttgg tattgaaggt ttaactacta gaaaattagc
     5641 tcaaaaatta ggtgttgaac aaccaacttt atattggcat gttaaaaata aaagagcttt
     5701 attagatgct ttagctattg aaatgttaga tagacatcat actcattttt gtccattaga
     5761 aggtgaatca tggcaagatt ttttaagaaa taatgctaaa tcatttagat gtgctttatt
     5821 atcacataga gatggtgcta aagttcattt aggtactaga ccaactgaaa aacaatatga
     5881 aactttagaa aatcaattag cttttttatg tcaacaaggt ttttcattag aaaatgcttt
     5941 atatgcttta tcagctgttg gtcattttac tttaggttgt gttttagaag atcaagaaca
     6001 tcaagttgct aaagaagaaa gagaaactcc aactactgat tcaatgccac cattattaag
     6061 acaagctatt gaattatttg atcatcaagg tgctgaacca gcttttttat ttggtttaga
     6121 attaattatt tgtggtttag aaaaacaatt aaaatgtgaa tcaggtggtt cgactttgtt
     6181 tggtggcgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
     6241 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
     6301 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
     6361 taaaattgat gatggtaata attcaaaacc attgtcacct ggttggacgg accaaactgc
     6421 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
     6481 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagagt aaaccgtgca
     6541 tcgctaccat tgttcaccac aaatgtttct aagagtgaaa ttctggaaat ctggaaatct
     6601 ggttttgtat tcttgttatt cttctttttg ttattacata tataacttgt tactttttta
     6661 aaaaaatctt tgtttatttt ataaatatat aaaactaaat ttaagaaaa
//
