LOCUS       V008252                 6334 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008252
VERSION     V008252
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6334)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6334)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(111..699)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(873..1730)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1731..1835)
                     /label="AmpR promoter"
     regulatory      1906..2640
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             2641..3258
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             3280..3621
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      3639..3945
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     CDS             4407..5300
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    5679..5949
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
ORIGIN
        1 gtaatcatgg tcataccgcg gaggcctggg cccgataacg caggaaagaa catgtgagca
       61 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      121 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      181 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      241 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      301 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      361 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      421 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      481 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      541 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      601 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      661 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      721 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      781 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      841 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      901 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      961 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1021 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1081 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1141 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1201 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1261 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1321 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1381 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1441 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1501 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1561 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1621 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1681 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1741 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1801 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1861 gacgtctaag aaccgcggag gcctgggccc ccgggtaccg agctcgccag cgagaaagag
     1921 gagtattggc attgttgggt agtagatgaa tgagtaatct tctagccaaa acaaaataaa
     1981 ccacaagaac agtaggcaaa actgagtact tgatagcgct tgtagtcttt gtatttaaaa
     2041 aaacctaatt gtccaaaaat tgaaaatcat gagttgcaaa tttgttgaaa tcaccaggta
     2101 aatagatcat ttagctatat cgccttatca ttcttcgtac aagaattata cacaaatacc
     2161 accaccacac cacccagaaa ttttacacgt gttttctaaa ttattacttt ccgtggccaa
     2221 tcaaattgaa atttttcttg ttcgaaacgg cagtgcctca aacattatag tagcaagtat
     2281 tgcctaaaga gtttccgtct cgtgcccccc ccctatcccc ttttgtaatt tgccaaaata
     2341 gctcaaaaag gataatcccg cacttttttt ttccgagttc tcgtcatgca agcaagctta
     2401 ttcagaattt tcagaggtgc gagtctcacc actaccactg cagctgcatc ttttacagca
     2461 acagcaacag caggtgccac cacggcaaag acattgtctg gatccactgt gctccgaaaa
     2521 ctgtataaaa ggacctattc atccctggtc ttatcttctc cagaattatt tttttttcat
     2581 cagtttaaca acaacaaacg ttattgtcat acaacaacaa caacaaatac aaaaacaatt
     2641 atgtcaagat tagataaatc aaaagttatt aattcagctt tagaattatt aaatgaagtt
     2701 ggtattgaag gtttaactac tagaaaatta gctcaaaaat taggtgttga acaaccaact
     2761 ttatattggc atgttaaaaa taaaagagct ttattagatg ctttagctat tgaaatgtta
     2821 gatagacatc atactcattt ttgtccatta gaaggtgaat catggcaaga ttttttaaga
     2881 aataatgcta aatcatttag atgtgcttta ttatcacata gagatggtgc taaagttcat
     2941 ttaggtacta gaccaactga aaaacaatat gaaactttag aaaatcaatt agctttttta
     3001 tgtcaacaag gtttttcatt agaaaatgct ttatatgctt tatcagctgt tggtcatttt
     3061 actttaggtt gtgttttaga agatcaagaa catcaagttg ctaaagaaga aagagaaact
     3121 ccaactactg attcaatgcc accattatta agacaagcta ttgaattatt tgatcatcaa
     3181 ggtgctgaac cagctttttt atttggttta gaattaatta tttgtggttt agaaaaacaa
     3241 ttaaaatgtg aatcaggtgg ttcgactttg tttggtggcg ccaattttaa tcaaagtggg
     3301 aatattgctg atagctcatt gtccttcact ttcactaaca gtagcaacgg tccgaacctc
     3361 ataacaactc aaacaaattc tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat
     3421 gataacttca tgaataatga aatcacggct agtaaaattg atgatggtaa taattcaaaa
     3481 ccattgtcac ctggttggac ggaccaaact gcgtataacg cgtttggaat cactacaggg
     3541 atgtttaata ccactacaat ggatgatgta tataactatc tattcgatga tgaagatacc
     3601 ccaccaaacc caaaaaaaga gtaaaccgtg catcgctacc attgttcacc acaaatgttt
     3661 ctaagagtga aattctggaa atctggaaat ctggttttgt attcttgtta ttcttctttt
     3721 tgttattaca tatataactt gttacttttt taaaaaaatc tttgtttatt ttataaatat
     3781 ataaaactaa atttaagaaa aagagaaaaa tgttttattt gagagattga aattttactt
     3841 gaatttagct tagcttttat aaagtattat tatgtaaaaa aacaaaacaa atatacatta
     3901 aaaagttaag actataaaat agccacccaa ggcatttcta tatctacagt ctgccagact
     3961 tgtatggtaa tccaaatggg tatggggtta gtagtaattt ggtagtgaaa accccggtag
     4021 acggagaatt aataaatacc ccaaaatcca ccacaggact cgacaggtac ctggaggatg
     4081 aggagacaga agttagtagt aacaatttgg ggacagaaga ggatacagtg acatcagagg
     4141 aagaagacag cgaaggtata gtcaaaaagc aataggacag caagacaatt tatttattta
     4201 atagtttttt ataaatgtaa aatctatcac aacatgtgta cttaattttc tgtggtaggg
     4261 gaggccattt cgttgaaacg tgtcgcgagc ggttgtgaga attgactcat tttgattatt
     4321 acgcaaccat tgaaattttt tatttttttt tggtgaagat ttttcccaca caacttcttc
     4381 ttttacttaa ccaccaacta ccgataatgg atttagtcaa tcatttacca gaccgtttgt
     4441 tatttgctgt tcctaaaaag ggcagattat acgaaaaatg ctgtaactta ttgagtggtg
     4501 ccgatataca gtttagaaga tctaatagat tagatatagc actttctaca aacttgccaa
     4561 ttgcattaat cttcttgcct gcagctgata tcccagtttt cgttggagaa ggtaattgtg
     4621 acttgggtat aactgggtta gaccaaatca aagaagctga acaattcgac aacatcgagg
     4681 acttgttgga tttgaaattt ggttcatgca aattgcagat ccaagttcca gcagatggcg
     4741 agtacgaaaa gccagaacag cttgttggaa agaaaattgt gtcttcattt acaaaattga
     4801 gtaccgacta tttcaaacaa ttgtcagaca aacctactaa tatcagatat gtcggtggtt
     4861 ccgttgaggc ttcttgtgcc ttgggtgttg ctgatgctat tgtcgatttg gttgaaagtg
     4921 gtgaaactat gaaagcagct gggttaaagg ccatagaaac catattggag acttcggctc
     4981 atttgatttc gtcaaagaaa agtaaattcc cagaaatggt caatataatc gtccaaagac
     5041 ttcaaggtgt tttagctgcc caggaatatg tcttgtgtaa ctacaatgcc ccaaaatcca
     5101 tccaagcaaa atgtttaacc attactccag gcagaagggc cgctactgtc tctactttgg
     5161 acaaacacag cgacgacgaa gaggactggg ttgccatctc atccatggtt aatagaaagg
     5221 aaatcggtaa tgtaatggac gaattgaaga aagctggtgc aaccgatata ttggtactcg
     5281 agatatcaaa ttgtagagtt taaaagaagt gatagtttct cataaatatt atacatatat
     5341 acttatagat ctacttcaat ttgactcaat ctgtcttata ccatctttat ctaccacctt
     5401 gatgtacaca cctttaaagt caataggcat tctcgtttgt aattccttga cacacatatc
     5461 cattaacttt aaaccatctt cgaccgtcat gtcctttcta taatgtttat ctaacaaaga
     5521 tgttgtgtaa aatgcagcgt agccatgagc accatcagtc agtcagtcag gcttggacag
     5581 catcagcagt agcgtattgt tcgaatgaac catcgtgagt gtaaccagac aagtcagctt
     5641 caccacagtt tggttcagca ccttgtcgag tttaccactc cctatcagtg atagagaaaa
     5701 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     5761 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     5821 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     5881 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     5941 tgatagagaa aagtgaaagt cgagctccaa acttcttttt tatttttcgc aatattgtcg
     6001 ataaattaaa tataaactac tcgattgagt aatagattca ataactatgg gaattactaa
     6061 caatgattta aggaaaaagc ttcacttaaa aataaagccc tttcaattaa agtgttgtcc
     6121 atgtttgtcg tttctgatgg gcttttctga aatataaata tagattcaaa tctcttgtat
     6181 tcaagtttta gatttttttt tatttctttt atatcattat caaatcaaat caacaaactc
     6241 tttcaacttc tcagaaagaa atcaatatca actacaataa aaccaactac gtctactact
     6301 acgttaactc ctacacacat acaaatataa atag
//
