LOCUS       V008253                 6318 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008253
VERSION     V008253
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6318)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6318)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             185..994
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    1376..1646
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     rep_origin      complement(2142..2730)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2904..3761)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3762..3866)
                     /label="AmpR promoter"
     regulatory      3943..4677
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     CDS             4678..5295
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             5317..5658
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      5676..5982
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
ORIGIN
        1 cctgcatatt atacagataa cataatgaaa gaaaaaatct ttttttttgt tcttcaatga
       61 tgatttcaac cattctttta aacattgatc aattcctgag caacaacccc atacacactg
      121 gtttatatat cgcccctttt acagttgaag aaagaaatag aaatagaaat agcaaacaaa
      181 agatatgaca gtcaacacta agacctatag tgagagagca gaaactcatg cctcaccagt
      241 agcacaacga ttatttcgat taatggaact gaagaaaacc aatttatgtg catcaattga
      301 tgttgatacc actaaggaat tccttgaatt aattgataaa ttgggtcctt atgtatgctt
      361 aatcaagact catattgata taatcaatga tttttcctat gaatccacta ttgaaccatt
      421 attagaactt tcacgtaaac atcaatttat gatttttgaa gatagaaaat ttgctgatat
      481 tggtaatacc gtgaagaaac aatatattgg tggagtttat aaaattagta gttgggcaga
      541 tattactaat gctcatggtg tcactgggaa tggagtagtt gaaggattaa aacagggagc
      601 taaagaaacc accaccaacc aagagccaag agggttattg atgttagctg aattatcatc
      661 agtgggatca ttagcatatg gagaatattc tcaaaaaact gttgaaattg ctaaatccga
      721 taaggaattt gttattggat ttattgccca acgtgatatg ggtggacaag aagaaggatt
      781 tgattggctt attatgacac ctggagttgg attagatgat aaaggtgatg gattaggaca
      841 acaatataga actgttgatg aagttgttag cactggaact gatattatca ttgttggtag
      901 aggattgttt ggtaaaggaa gagatccaga tattgaaggt aaaaggtata gagatgctgg
      961 ttggaatgct tatttgaaaa aaactggcca attataaatg tgaaggggga gattttcact
     1021 ttattagatt tgtatatatg tataataaat aaataaataa gttaaataaa taattaaata
     1081 agggtggtaa ttattactat ttacaatcaa aggtggtcct tctagaaatc atgacagggg
     1141 ggtcaaatgt tttacgcttc aagaacatca ttataatctt caaaattatc acttaattta
     1201 gttctcatta tttcaaatac taataaggca atcgaagtag ctgggatagt agcagtcagt
     1261 cagtcaggct tggacagcat cagcagtagc gtattgttcg aatgaaccat cgtgagtgta
     1321 accagacaag tcagcttcac cacagtttgg ttcagcacct tgtcgagttt accactccct
     1381 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     1441 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     1501 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     1561 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     1621 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctccaaact tcttttttat
     1681 ttttcgcaat attgtcgata aattaaatat aaactactcg attgagtaat agattcaata
     1741 actatgggaa ttactaacaa tgatttaagg aaaaagcttc acttaaaaat aaagcccttt
     1801 caattaaagt gttgtccatg tttgtcgttt ctgatgggct tttctgaaat ataaatatag
     1861 attcaaatct cttgtattca agttttagat ttttttttat ttcttttata tcattatcaa
     1921 atcaaatcaa caaactcttt caacttctca gaaagaaatc aatatcaact acaataaaac
     1981 caactacgtc tactactacg ttaactccta cacacataca aatataaata ggtaatcatg
     2041 gtcataccgc ggaggcctgg gcccgataac gcaggaaaga acatgtgagc aaaaggccag
     2101 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2161 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2221 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2281 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     2341 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2401 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2461 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2521 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2581 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2641 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2701 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2761 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2821 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2881 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2941 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3001 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3061 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3121 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3181 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3241 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3301 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3361 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3421 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3481 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3541 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3601 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3661 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3721 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3781 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3841 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     3901 gaacccggga agcttggatc ccttaagaca gtctgccaga ctgccagcga gaaagaggag
     3961 tattggcatt gttgggtagt agatgaatga gtaatcttct agccaaaaca aaataaacca
     4021 caagaacagt aggcaaaact gagtacttga tagcgcttgt agtctttgta tttaaaaaaa
     4081 cctaattgtc caaaaattga aaatcatgag ttgcaaattt gttgaaatca ccaggtaaat
     4141 agatcattta gctatatcgc cttatcattc ttcgtacaag aattatacac aaataccacc
     4201 accacaccac ccagaaattt tacacgtgtt ttctaaatta ttactttccg tggccaatca
     4261 aattgaaatt tttcttgttc gaaacggcag tgcctcaaac attatagtag caagtattgc
     4321 ctaaagagtt tccgtctcgt gccccccccc tatccccttt tgtaatttgc caaaatagct
     4381 caaaaaggat aatcccgcac tttttttttc cgagttctcg tcatgcaagc aagcttattc
     4441 agaattttca gaggtgcgag tctcaccact accactgcag ctgcatcttt tacagcaaca
     4501 gcaacagcag gtgccaccac ggcaaagaca ttgtctggat ccactgtgct ccgaaaactg
     4561 tataaaagga cctattcatc cctggtctta tcttctccag aattattttt ttttcatcag
     4621 tttaacaaca acaaacgtta ttgtcataca acaacaacaa caaatacaaa aacaattatg
     4681 tcaagattag ataaatcaaa agttattaat tcagctttag aattattaaa tgaagttggt
     4741 attgaaggtt taactactag aaaattagct caaaaattag gtgttgaaca accaacttta
     4801 tattggcatg ttaaaaataa aagagcttta ttagatgctt tagctattga aatgttagat
     4861 agacatcata ctcatttttg tccattagaa ggtgaatcat ggcaagattt tttaagaaat
     4921 aatgctaaat catttagatg tgctttatta tcacatagag atggtgctaa agttcattta
     4981 ggtactagac caactgaaaa acaatatgaa actttagaaa atcaattagc ttttttatgt
     5041 caacaaggtt tttcattaga aaatgcttta tatgctttat cagctgttgg tcattttact
     5101 ttaggttgtg ttttagaaga tcaagaacat caagttgcta aagaagaaag agaaactcca
     5161 actactgatt caatgccacc attattaaga caagctattg aattatttga tcatcaaggt
     5221 gctgaaccag cttttttatt tggtttagaa ttaattattt gtggtttaga aaaacaatta
     5281 aaatgtgaat caggtggttc gactttgttt ggtggcgcca attttaatca aagtgggaat
     5341 attgctgata gctcattgtc cttcactttc actaacagta gcaacggtcc gaacctcata
     5401 acaactcaaa caaattctca agcgctttca caaccaattg cctcctctaa cgttcatgat
     5461 aacttcatga ataatgaaat cacggctagt aaaattgatg atggtaataa ttcaaaacca
     5521 ttgtcacctg gttggacgga ccaaactgcg tataacgcgt ttggaatcac tacagggatg
     5581 tttaatacca ctacaatgga tgatgtatat aactatctat tcgatgatga agatacccca
     5641 ccaaacccaa aaaaagagta aaccgtgcat cgctaccatt gttcaccaca aatgtttcta
     5701 agagtgaaat tctggaaatc tggaaatctg gttttgtatt cttgttattc ttctttttgt
     5761 tattacatat ataacttgtt acttttttaa aaaaatcttt gtttatttta taaatatata
     5821 aaactaaatt taagaaaaag agaaaaatgt tttatttgag agattgaaat tttacttgaa
     5881 tttagcttag cttttataaa gtattattat gtaaaaaaac aaaacaaata tacattaaaa
     5941 agttaagact ataaaatagc cacccaaggc atttctatat ctacagtctg ccagactcaa
     6001 aaccggatct tcagaacaaa atccatttca aaaaaaactt atgaattatt taaacagttt
     6061 gaactgagag taattacaaa gtactaatag gaattgattt ggatggtata aacggaaaca
     6121 aaaaaaagag ctggtactac tttctttaaa attattttat tatttgattt tatttaatag
     6181 tatatattat attttgaacg tagattattt tgttgaaagt tgctgtagtg ccattgattc
     6241 gtaacactaa ttctgtatta gtcattcctc ttgtttgata gtatccaaaa aaacggctat
     6301 ttttttgcaa tcttattt
//
