LOCUS       40924_12975        8200 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC29, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 8200)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8200)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(292..880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1054..1911)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1912..2016)
                     /label=AmpR promoter
     misc_feature    2118..2562
                     /note="5' region of EAF7 genes of Candida albicans; 5' of
                     EAF7"
     regulatory      2578..4050
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
                     /regulatory_class="promoter"
     regulatory      4064..4370
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     protein_bind    4407..4440
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT); loxP"
     CDS             5522..6916
                     /codon_start=1
                     /product="Arg4"
                     /label=Arg4
                     /protein_id="AVR52630.1"
                     /translation="MSQQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADLTGTK
                     VYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGEIIGK
                     NISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMPGYTH
                     LQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREFLAKG
                     LGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLADAYS
                     TGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLFDALT
                     TVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISGECVR
                     KAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLKQLEN
                     LKSILN"
     protein_bind    complement(7734..7767)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taatatcaaa aagagaacaa gacagagtgt caaatttgac gacaagcctg cagagccctc
       61 cgatacaggt tctagaaaaa gacgagctcc gccaggatca gtatctcctc cacatcctcc
      121 taaaacacga agacgtacaa gatcagtaac ccatgaaata gaggaagagg atgcttcgac
      181 agtaatcatg gtcataccgc ggaggcctgg gcccgataac gcaggaaaga acatgtgagc
      241 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      301 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      361 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      421 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      481 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      541 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      601 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      661 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      721 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      781 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      841 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      901 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      961 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1021 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1081 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1141 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1201 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1261 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1321 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1381 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1441 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1501 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1561 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1621 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1681 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1741 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1801 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1861 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     1921 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     1981 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     2041 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     2101 gccaggtcga ctctagacat tggtccatcg agaatgagat aaagctattc agcttattgt
     2161 gcgattataa gcccgctggt aaaaacaaac aacacaatat taaaatcatt atcaacaata
     2221 ttaatgaatc tttagactct aaggaggaac ccttcagcga atatgacgtg tggaataaat
     2281 tgaggtcttt atatgatcta gaaaagattg accagattga ggaattactg actgagaaca
     2341 ccagtacaga agaaaccaca aaccaagtgc tgtctggatc tgcaaatatc acgaaggaca
     2401 ccaagaacgc taaaactact ttggatgcaa aagagccaat tcaagataag aaagaaatgg
     2461 ataaagagaa atcagaatcg tcatcattgt ctacccctga accaccacaa agaagaacta
     2521 gaagtgccag agatacaaca aaatcgaaac gtaacttgcg tgcgatccgt ctaggattga
     2581 acgatgatat taagcgagaa ttcacatttt tctagaggaa tcacagttac aattgtagag
     2641 gaaaccgaac tagtaaaact agcacccaac acttctaaac aaaccaatca attcaatggt
     2701 ataacaaatc gtctaccaga agttatgcta atatgaaaca tcaattgaca acatggacat
     2761 caactgaagc tttaacaaat gaataaaaaa aaaagcccat acaatagacc attggtcata
     2821 gtttcatttg cccccgcccc acttttattg ttgagttgaa taattgtgag catcagctga
     2881 ttttaaaatt ataaattttt tttttcgtcc attgttaatt ctattttagt tttttcttct
     2941 ccatcgtctc cgaattattc cggaagctgg tagcggataa gttttagaat cattgagcct
     3001 cttttcaatg ccagatgtta tctgcaactg atggttagtt agttagtata agacttaaat
     3061 tctcatatag gtcatgtgca cggacaagct cattgagtga cgaaaagtca aatttaaact
     3121 tctctaaaca aatttcagac aatagtccga aaaaaaaaaa caaaacccat tattgtcgac
     3181 atgtcaaagg attcaacggt tgagatggag ccgtcaaata tccaaaacac tcttctttca
     3241 actaatttgc cgtaaactat ctccatcgta cgattaagat aaaaaaaaac aattatttct
     3301 tacaatattt gatagagacc caatgcaaag ccagcgagaa agaggagtat tggcattgtt
     3361 gggtagtaga tgaatgagta atcttctagc caaaacaaaa taaaccacaa gaacagtagg
     3421 caaaactgag tacttgatag cgcttgtagt ctttgtattt aaaaaaacct aattgtccaa
     3481 aaattgaaaa tcatgagttg caaatttgtt gaaatcacca ggtaaataga tcatttagct
     3541 atatcgcctt atcattcttc gtacaagaat tatacacaaa taccaccacc acaccaccca
     3601 gaaattttac acgtgttttc taaattatta ctttccgtgg ccaatcaaat tgaaattttt
     3661 cttgttcgaa acggcagtgc ctcaaacatt atagtagcaa gtattgccta aagagtttcc
     3721 gtctcgtgcc ccccccctat ccccttttgt aatttgccaa aatagctcaa aaaggataat
     3781 cccgcacttt ttttttccga gttctcgtca tgcaagcaag cttattcaga attttcagag
     3841 gtgcgagtct caccactacc actgcagctg catcttttac agcaacagca acagcaggtg
     3901 ccaccacggc aaagacattg tctggatcca ctgtgctccg aaaactgtat aaaaggacct
     3961 attcatccct ggtcttatct tctccagaat tatttttttt tcatcagttt aacaacaaca
     4021 aacgttattg tcatacaaca acaacaacaa ccgtgcatcg ctaccattgt tcaccacaaa
     4081 tgtttctaag agtgaaattc tggaaatctg gaaatctggt tttgtattct tgttattctt
     4141 ctttttgtta ttacatatat aacttgttac ttttttaaaa aaatctttgt ttattttata
     4201 aatatataaa actaaattta agaaaaagag aaaaatgttt tatttgagag attgaaattt
     4261 tacttgaatt tagcttagct tttataaagt attattatgt aaaaaaacaa aacaaatata
     4321 cattaaaaag ttaagactat aaaatagcca cccaaggcat ttctatatct acagtctgcc
     4381 agacttgcca cctgacgtct aagaaaataa cttcgtatag catacattat acgaagttat
     4441 agctcggatc cactagtaac ggccgccagt gtgctggaat tcgcccttaa gaacatttct
     4501 gtaccgcaaa tgtatcgact ttttcgatgg tgtgttattg ttgttttgtt gcgaaatata
     4561 atagcttctg ctttatatcc tttgtgttga tccttcccct cctttcatac ttgtaaaatt
     4621 acaattggta ttttcaacca atattaccac acgtcatgca ctaactcaaa tgggcaaaaa
     4681 aaagagagat aggaattacg tcattttatt atgtttttac gtggactaga aggttaagat
     4741 aatttctggt tattttgttg ataattgagt tttgttcgac ctccaccaat ctttaaactc
     4801 tcgttgagta ttcattttaa tgattttttg aaatcagaag gggtgtttct aacccaggat
     4861 tgttttattg agaggttgaa acatcaatgt gttagtttat tgatttgggt tggtttaata
     4921 gaaaaatatc agtaggtgtt caataattgt agtttatttt cttggctaaa attgttgcga
     4981 aaaattgaca aaataataac aaaaagtatt ttagatgcta aaacataata gtaggaatat
     5041 ctacctgaac catttacatt caacaactgg caagaacctt cctaataatt tgcaagagta
     5101 atcacaatta aaccaaaatc aaagtttacc gtgtatatag ttggacagaa agctgtcaca
     5161 ttggtttaat acatagttga atatgtatgc atgaaacaac aacaatacaa ttacagctca
     5221 tgatccttca attaatttca acattcaaac aacataacac cttgtgtaaa tgaggttatg
     5281 agtacatgtg aacaacaaat cttatgacta aactcgggtc gtgtactttt gacgattgag
     5341 tcatttttac caattaggca attaattttt gctaaatttt cattaaccgc gctggccatc
     5401 ccaacaaaca atataaaagc tgacaacgaa aaaaaaaaaa aaaaaaaaaa aactccaatt
     5461 ctgaaaaatt tttcttcttc ttcatactaa acaaaacaac tataaagata atcaattaat
     5521 aatgtcacaa caacctagtg aaaataaatt atggggtggt cgattcactg gcgccactga
     5581 tccattaatg gatttataca atgcttcatt accttatgat aaagttatgt atgatgcaga
     5641 tttaactggt actaaagtat atactcaagg attgaacaaa ttaggattaa ttaccactga
     5701 agaattgaat ctgattcatc aaggtttaga acaaattaga caagaatggc atgataataa
     5761 atttattatt aaagctggtg atgaagatat tcatactgct aatgaacgta gattaggaga
     5821 aatcattggg aaaaacattt ctggtaaagt tcatactggt agatccagaa atgatcaagt
     5881 tgctactgat atgagaattt ttgttcgtga atcattactt aatttaagta agattttaca
     5941 tcaattcatt acagcaattc ttgaacgagc tcataaagaa attgatgtat taatgcctgg
     6001 ttatactcat ttacaaaaag ctcaacctat tagatgggct cattggttaa gttcatatgc
     6061 aacttatttc actgaagatt ataaacgttt acaagaaatc attactcgtg ttaatcaatc
     6121 tccattagga tctggtgctt tagctggtca tccctacggt attgatcgtg aatttttagc
     6181 taaaggatta ggatttgatg gtgttattgg gaattcttta actgcagttt ctgatcgtga
     6241 ttttgttgtt gaaagtttat tttggagtac attatttatg aatcatattt ctcgattttc
     6301 agaagattta attatttatt ctagtggtga atttggattt ataaagttag ctgatgctta
     6361 ctctactggt tcatcattaa tgccacaaaa gaaaaatcct gattcattag aattattacg
     6421 tgggaaaagt ggcagagtgt ttggtcagtt atctgggttt ttaatgtcaa ttaaatccat
     6481 tccatcaacc tataataaag atatgcaaga agataaagaa ccattatttg atgcattgac
     6541 taccgtagaa cattcaattt taattgctac tggtgtgatt tctactttac tgattgataa
     6601 acaaaatatg gaaaaagctt taactatgga tatgttagct actgatttag cagattattt
     6661 agttagaaaa ggtgttccat ttagagaaac tcatcatatt tctggtgaat gtgttagaaa
     6721 agctgaagaa gaaaaattgt cgggaattga ccaattatca tttgaacaat ttcaacaaat
     6781 tgattcacgt tttgaaaaag atgttatgga aacttttgat tttgaagcta gtgtggaaag
     6841 aagagatgct attggtggta ctgctaagag cgctgtttta aaacagttag aaaatttgaa
     6901 atcaatttta aattaattta taaataatca tatagtattc aatgttttgt tttgtttttt
     6961 tttttttttt tatggaaatg tacaaacagt atataatcaa ttaatagaaa gataccctgt
     7021 attccattgt atcgcctttg ggatgtctat aataccaact ttcaagaatg agaaattaaa
     7081 caacaatttc tttcaccaat caactcgtct atattcaata aactcattct ggtcttttca
     7141 aatatttaat gttatcctga tcaaacttac aaatcaaaaa tgtatcaaaa tcattcatta
     7201 gttcttcttc ttttgttatc tttgtcacta tatttctctc catccagacc aaaaatactt
     7261 ccattgtgaa gatcgtctga atcataatca gcaactacct cttccatttc agagaattca
     7321 gatttttcaa aataggtatc acgggccaag tacacacaag acaaacgata cgagtgctgg
     7381 gatatttctt tcaatgtatc actaaattcg ttgagattta aagagagccc tttgactgct
     7441 ttcattactt tcctcacatc atgagcatcc acattcaaat ttcctgtagc cctttattct
     7501 tagttttctt ttcttattct tttcttcgta cattgaaatc acaaatggtg atatcaaacg
     7561 aaggtcacac tgacttatgt ctaatacctc caatgatgat actggtaact taaccaggtc
     7621 tactcttttc aaacgatttc tcattagtgt caatttacgt aggttcttca acctttcaaa
     7681 cgatgcaaat agcttttgta agggcgaatt ctgcagatat ccatcacact ggtataactt
     7741 cgtatagcat acattatacg aagttatggt aatacggtta tccacagaat caggggataa
     7801 cgcaggaaag aacatgtgag caaaaggcct aactagatag cagtcagtca gtcagcaaag
     7861 ataaggggga tgagacaatt gctaaaatta agaaagaaaa cacttctgag caagaagaag
     7921 ataaaaacaa aaagcagaca gaagaagagg cagaagaaga ggaagaagaa ggtgatcatg
     7981 ttagtggaga tgaaagggaa agcaaaaata aggacactag tgaagatgag gatggtgaag
     8041 ataatgaaga tcaagatgaa gatgatgttg aagtaattga ggacagccat actgataaag
     8101 aaaatgaatc tagttcagaa gaatcatcac ctgaaccaat gactaaacga acgagacaaa
     8161 gaacaaggtc cacccatgaa aaaacagaac acacgtcacc
//
