LOCUS       V008255                 7255 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008255
VERSION     V008255
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7255)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7255)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(352..940)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1114..1971)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1972..2076)
                     /label="AmpR promoter"
     misc_feature    2178..2622
                     /note="5' region of EAF7 genes of Candida albicans; 5' of
                     EAF7"
     regulatory      2638..4110
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     regulatory      4124..4430
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     protein_bind    4467..4500
                     /label="Cre recombinase binding site"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT); loxP"
     CDS             5576..6469
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(6849..6882)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gactaaacga acgagacaaa gaacaaggtc cacccatgaa aaaacagaac acacgtcacc
       61 taatatcaaa aagagaacaa gacagagtgt caaatttgac gacaagcctg cagagccctc
      121 cgatacaggt tctagaaaaa gacgagctcc gccaggatca gtatctcctc cacatcctcc
      181 taaaacacga agacgtacaa gatcagtaac ccatgaaata gaggaagagg atgcttcgac
      241 agtaatcatg gtcataccgc ggaggcctgg gcccgataac gcaggaaaga acatgtgagc
      301 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      361 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      421 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      481 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      541 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      601 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      661 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      721 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      781 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      841 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      901 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      961 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1021 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1081 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1141 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1201 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1261 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1321 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1381 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1441 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1501 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1561 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1621 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1681 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1741 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1801 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1861 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1921 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     1981 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     2041 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     2101 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     2161 gccaggtcga ctctagacat tggtccatcg agaatgagat aaagctattc agcttattgt
     2221 gcgattataa gcccgctggt aaaaacaaac aacacaatat taaaatcatt atcaacaata
     2281 ttaatgaatc tttagactct aaggaggaac ccttcagcga atatgacgtg tggaataaat
     2341 tgaggtcttt atatgatcta gaaaagattg accagattga ggaattactg actgagaaca
     2401 ccagtacaga agaaaccaca aaccaagtgc tgtctggatc tgcaaatatc acgaaggaca
     2461 ccaagaacgc taaaactact ttggatgcaa aagagccaat tcaagataag aaagaaatgg
     2521 ataaagagaa atcagaatcg tcatcattgt ctacccctga accaccacaa agaagaacta
     2581 gaagtgccag agatacaaca aaatcgaaac gtaacttgcg tgcgatccgt ctaggattga
     2641 acgatgatat taagcgagaa ttcacatttt tctagaggaa tcacagttac aattgtagag
     2701 gaaaccgaac tagtaaaact agcacccaac acttctaaac aaaccaatca attcaatggt
     2761 ataacaaatc gtctaccaga agttatgcta atatgaaaca tcaattgaca acatggacat
     2821 caactgaagc tttaacaaat gaataaaaaa aaaagcccat acaatagacc attggtcata
     2881 gtttcatttg cccccgcccc acttttattg ttgagttgaa taattgtgag catcagctga
     2941 ttttaaaatt ataaattttt tttttcgtcc attgttaatt ctattttagt tttttcttct
     3001 ccatcgtctc cgaattattc cggaagctgg tagcggataa gttttagaat cattgagcct
     3061 cttttcaatg ccagatgtta tctgcaactg atggttagtt agttagtata agacttaaat
     3121 tctcatatag gtcatgtgca cggacaagct cattgagtga cgaaaagtca aatttaaact
     3181 tctctaaaca aatttcagac aatagtccga aaaaaaaaaa caaaacccat tattgtcgac
     3241 atgtcaaagg attcaacggt tgagatggag ccgtcaaata tccaaaacac tcttctttca
     3301 actaatttgc cgtaaactat ctccatcgta cgattaagat aaaaaaaaac aattatttct
     3361 tacaatattt gatagagacc caatgcaaag ccagcgagaa agaggagtat tggcattgtt
     3421 gggtagtaga tgaatgagta atcttctagc caaaacaaaa taaaccacaa gaacagtagg
     3481 caaaactgag tacttgatag cgcttgtagt ctttgtattt aaaaaaacct aattgtccaa
     3541 aaattgaaaa tcatgagttg caaatttgtt gaaatcacca ggtaaataga tcatttagct
     3601 atatcgcctt atcattcttc gtacaagaat tatacacaaa taccaccacc acaccaccca
     3661 gaaattttac acgtgttttc taaattatta ctttccgtgg ccaatcaaat tgaaattttt
     3721 cttgttcgaa acggcagtgc ctcaaacatt atagtagcaa gtattgccta aagagtttcc
     3781 gtctcgtgcc ccccccctat ccccttttgt aatttgccaa aatagctcaa aaaggataat
     3841 cccgcacttt ttttttccga gttctcgtca tgcaagcaag cttattcaga attttcagag
     3901 gtgcgagtct caccactacc actgcagctg catcttttac agcaacagca acagcaggtg
     3961 ccaccacggc aaagacattg tctggatcca ctgtgctccg aaaactgtat aaaaggacct
     4021 attcatccct ggtcttatct tctccagaat tatttttttt tcatcagttt aacaacaaca
     4081 aacgttattg tcatacaaca acaacaacaa ccgtgcatcg ctaccattgt tcaccacaaa
     4141 tgtttctaag agtgaaattc tggaaatctg gaaatctggt tttgtattct tgttattctt
     4201 ctttttgtta ttacatatat aacttgttac ttttttaaaa aaatctttgt ttattttata
     4261 aatatataaa actaaattta agaaaaagag aaaaatgttt tatttgagag attgaaattt
     4321 tacttgaatt tagcttagct tttataaagt attattatgt aaaaaaacaa aacaaatata
     4381 cattaaaaag ttaagactat aaaatagcca cccaaggcat ttctatatct acagtctgcc
     4441 agacttgcca cctgacgtct aagaaaataa cttcgtatag catacattat acgaagttat
     4501 agctcggatc cactagtaac ggccgccagt gtgctggaat tcgcccttgt ttgataccat
     4561 ggcaagcata ctagaattat tatacgagct gggtgtgaaa tgtgccaccg tttatgcatt
     4621 ttcgatcgaa aacttcaaga gactgcaata tgaggtcaaa tggttgatgg aattagccaa
     4681 atccaagttt acgcaaataa accaacatgg attgttgtgt gaggagtatg gggttaaaat
     4741 cagaatatta ggaaatacca aacttttgcc gaaagatgtg ttagagatcc ttgaaagaac
     4801 cgaagaaata actaaaaaca ataagcgtgc tgtgttgaat gtctgcttcc catatacatc
     4861 aagagacgag ataacccatc tgatcaagtc agtagtagac gaagcgacga aaggtggtat
     4921 tgaaataacc gaggaggcta tcgacaagca tctttatacg aaagatgtgc ccccattgga
     4981 tttactagta agaacatcag gaacatttag actttcagac tttcttttat ggcaaacggt
     5041 gtcgccagat tgtgcagttg tgtttgtcga caagttatgg ccggcattct agaccatggg
     5101 atatgctcaa gattttactc aactgggggt ttaacaaata catgtatgga aacccaaatg
     5161 gatatggggt tagtaataac ttggtagtga agagccaaat ggaagaagaa ttaataaaca
     5221 caccaaatgg agctacaggg cttgaccggt acctggagga cgaagagacc gaagttagta
     5281 gtaacaattt aggaactgaa gaggatacag tgacatccga agaagaagat gttgaagata
     5341 taggcaaaaa gtaataggac agtattttat ttattcaata gttttttttt tttttttgaa
     5401 acgtacacct tgtttatttc aggtgtttct ttgaaacgtg tctcaaagcg ataacgggta
     5461 caaaaaaaaa tgtattgagt cattgaaatt tttttatttt tttttttttt tggtgaagat
     5521 ttttcccaca ccaaatatat accacttttc ttctaacacc acctacatat tcactatgga
     5581 tttagtcaat catttgcccg atcgtttgtt atttgctgtt cccaaaaagg gcagattata
     5641 tgaaaaatgc tgcagcttat tgagcggtgc cgatatacag tttagaagat ctaatagatt
     5701 ggatattgcc ctttccacaa acttgccaat tgcattaatc tttttgcctg cagccgatat
     5761 tccagttttc gttggagaag gtaattgtga cttgggtata actgggttag accagatcaa
     5821 agaagcagac caattcgaca acattgagga tttgttggat ttgaagtttg gctcatgtaa
     5881 attgcagatc caagttcctg caaatggcga gtacgaaaaa cccgaacaac tcgttggaaa
     5941 gaagattgta tcctccttta caaagttgag tactgactat ttcaagaaat tatccgacaa
     6001 acctacgaat attagatacg ttggtggttc agttgaggct tcttgcgcat tgggtgttgc
     6061 cgacgccatt gtcgatttag ttgaaagtgg agagaccatg aaagctgctg gattaaaggc
     6121 gatcgaaacc atattggaaa cttccgctca tttgatttcc tccaagaaaa ccaaatttcc
     6181 agaaatggtc aacataattg tccaaagact tcaaggtgtt ttagcggctc aggaatatgt
     6241 cttgtgtaac tacaatgctc caaaatccat tcaatcaaag tgcttaacca ttactccagg
     6301 tagaagggca gccactgtct ccactttgga taaacacagt gacgacgaag atgactgggt
     6361 tgccatttct tccatggtta acagaaagga aattggtaat gtaatggatg aattgaagaa
     6421 agctggcgca acagatatat tggtgctcga gatatcaaat tgtagagttt aataaaatac
     6481 tatatataca tacttataga tttacttcaa tttgactcaa tctgtcttat accatcttta
     6541 tctaccacct tgatgtacac acctttaaaa tcaatgggca ttctcgtttg taattctttg
     6601 acacacatgt ccattaactt caaaccatct tcaactgtca tgtcctttct ataatgttta
     6661 tctaacaagg atgttgtgta aaatgctgcg tagccatgag caccataagg acgttcaact
     6721 tgcgtaccta aataatctat ccaattcaac gacggctgat ttgtctttac atcgtatcct
     6781 ccaattaaac agttcacctg gtacggtttt ctagaagggc gaattctgca gatatccatc
     6841 acactggtat aacttcgtat agcatacatt atacgaagtt atggtaatac ggttatccac
     6901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggcctaacta gatagcagtc
     6961 agtcagtcag caaagataag ggggatgaga caattgctaa aattaagaaa gaaaacactt
     7021 ctgagcaaga agaagataaa aacaaaaagc agacagaaga agaggcagaa gaagaggaag
     7081 aagaaggtga tcatgttagt ggagatgaaa gggaaagcaa aaataaggac actagtgaag
     7141 atgaggatgg tgaagataat gaagatcaag atgaagatga tgttgaagta attgaggaca
     7201 gccatactga taaagaaaat gaatctagtt cagaagaatc atcacctgaa ccaat
//
