LOCUS       V008258                 7213 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008258
VERSION     V008258
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7213)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7213)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(239..827)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1001..1858)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1859..1963)
                     /label="AmpR promoter"
     misc_feature    2065..2460
                     /note="5' region of ADE2 genes of Candida albicans; 5' of
                     ADE2"
     regulatory      2476..3948
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     regulatory      3962..4268
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     protein_bind    4305..4338
                     /label="Cre recombinase binding site"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT); loxP"
     CDS             5414..6307
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(6687..6720)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gaactcacaa tcgtaagtgc acacagaact cctcatagaa tgtctgagta tgctattgaa
       61 gcaccaaagc gtggcttgaa atgtatcatt gctggtgcag gtggtgctgc tcatttgcca
      121 ggtatggtgt aatcatggtc ataccgcgga ggcctgggcc cgataacgca ggaaagaaca
      181 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      241 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      301 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      361 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      421 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      481 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      541 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      601 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      661 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      721 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      781 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      841 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      901 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
      961 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1021 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1081 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1141 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1201 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1261 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1321 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1381 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1441 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1501 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1561 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1621 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1681 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     1741 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     1801 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     1861 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1921 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     1981 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     2041 tcacgaggcc aggtcgactc tagaaggtgg ccaattaggt cgtatgattg ttgaagcagc
     2101 acatagattg aatatcaaga ccgtaatttt ggacgcagcc aaatcaccag ctaaacagat
     2161 caatgctcta gacgatcacg ttgatggatc gttcacaaac tatgattcga ttgtgaaatt
     2221 agctgaaaag gcagacgtat taactgttga aatcgaacat gttgatgttg atgctttaat
     2281 caaagttcaa gagaaattcc ctaaagttga aatatatcca ttacctgaaa ccataagatt
     2341 gattcaagat aaatatttac agaaaaatca tttaatcaaa cacgatgttg cagttacaga
     2401 atctgttgca gttgaaacca acacagtcga tgacttgtta cacattgggg aaaagtttgg
     2461 cgatccgtct aggattgaac gatgatatta agcgagaatt cacatttttc tagaggaatc
     2521 acagttacaa ttgtagagga aaccgaacta gtaaaactag cacccaacac ttctaaacaa
     2581 accaatcaat tcaatggtat aacaaatcgt ctaccagaag ttatgctaat atgaaacatc
     2641 aattgacaac atggacatca actgaagctt taacaaatga ataaaaaaaa aagcccatac
     2701 aatagaccat tggtcatagt ttcatttgcc cccgccccac ttttattgtt gagttgaata
     2761 attgtgagca tcagctgatt ttaaaattat aaattttttt tttcgtccat tgttaattct
     2821 attttagttt tttcttctcc atcgtctccg aattattccg gaagctggta gcggataagt
     2881 tttagaatca ttgagcctct tttcaatgcc agatgttatc tgcaactgat ggttagttag
     2941 ttagtataag acttaaattc tcatataggt catgtgcacg gacaagctca ttgagtgacg
     3001 aaaagtcaaa tttaaacttc tctaaacaaa tttcagacaa tagtccgaaa aaaaaaaaca
     3061 aaacccatta ttgtcgacat gtcaaaggat tcaacggttg agatggagcc gtcaaatatc
     3121 caaaacactc ttctttcaac taatttgccg taaactatct ccatcgtacg attaagataa
     3181 aaaaaaacaa ttatttctta caatatttga tagagaccca atgcaaagcc agcgagaaag
     3241 aggagtattg gcattgttgg gtagtagatg aatgagtaat cttctagcca aaacaaaata
     3301 aaccacaaga acagtaggca aaactgagta cttgatagcg cttgtagtct ttgtatttaa
     3361 aaaaacctaa ttgtccaaaa attgaaaatc atgagttgca aatttgttga aatcaccagg
     3421 taaatagatc atttagctat atcgccttat cattcttcgt acaagaatta tacacaaata
     3481 ccaccaccac accacccaga aattttacac gtgttttcta aattattact ttccgtggcc
     3541 aatcaaattg aaatttttct tgttcgaaac ggcagtgcct caaacattat agtagcaagt
     3601 attgcctaaa gagtttccgt ctcgtgcccc ccccctatcc ccttttgtaa tttgccaaaa
     3661 tagctcaaaa aggataatcc cgcacttttt ttttccgagt tctcgtcatg caagcaagct
     3721 tattcagaat tttcagaggt gcgagtctca ccactaccac tgcagctgca tcttttacag
     3781 caacagcaac agcaggtgcc accacggcaa agacattgtc tggatccact gtgctccgaa
     3841 aactgtataa aaggacctat tcatccctgg tcttatcttc tccagaatta tttttttttc
     3901 atcagtttaa caacaacaaa cgttattgtc atacaacaac aacaacaacc gtgcatcgct
     3961 accattgttc accacaaatg tttctaagag tgaaattctg gaaatctgga aatctggttt
     4021 tgtattcttg ttattcttct ttttgttatt acatatataa cttgttactt ttttaaaaaa
     4081 atctttgttt attttataaa tatataaaac taaatttaag aaaaagagaa aaatgtttta
     4141 tttgagagat tgaaatttta cttgaattta gcttagcttt tataaagtat tattatgtaa
     4201 aaaaacaaaa caaatataca ttaaaaagtt aagactataa aatagccacc caaggcattt
     4261 ctatatctac agtctgccag acttgccacc tgacgtctaa gaaaataact tcgtatagca
     4321 tacattatac gaagttatag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc
     4381 gcccttgttt gataccatgg caagcatact agaattatta tacgagctgg gtgtgaaatg
     4441 tgccaccgtt tatgcatttt cgatcgaaaa cttcaagaga ctgcaatatg aggtcaaatg
     4501 gttgatggaa ttagccaaat ccaagtttac gcaaataaac caacatggat tgttgtgtga
     4561 ggagtatggg gttaaaatca gaatattagg aaataccaaa cttttgccga aagatgtgtt
     4621 agagatcctt gaaagaaccg aagaaataac taaaaacaat aagcgtgctg tgttgaatgt
     4681 ctgcttccca tatacatcaa gagacgagat aacccatctg atcaagtcag tagtagacga
     4741 agcgacgaaa ggtggtattg aaataaccga ggaggctatc gacaagcatc tttatacgaa
     4801 agatgtgccc ccattggatt tactagtaag aacatcagga acatttagac tttcagactt
     4861 tcttttatgg caaacggtgt cgccagattg tgcagttgtg tttgtcgaca agttatggcc
     4921 ggcattctag accatgggat atgctcaaga ttttactcaa ctgggggttt aacaaataca
     4981 tgtatggaaa cccaaatgga tatggggtta gtaataactt ggtagtgaag agccaaatgg
     5041 aagaagaatt aataaacaca ccaaatggag ctacagggct tgaccggtac ctggaggacg
     5101 aagagaccga agttagtagt aacaatttag gaactgaaga ggatacagtg acatccgaag
     5161 aagaagatgt tgaagatata ggcaaaaagt aataggacag tattttattt attcaatagt
     5221 tttttttttt tttttgaaac gtacaccttg tttatttcag gtgtttcttt gaaacgtgtc
     5281 tcaaagcgat aacgggtaca aaaaaaaatg tattgagtca ttgaaatttt tttatttttt
     5341 tttttttttg gtgaagattt ttcccacacc aaatatatac cacttttctt ctaacaccac
     5401 ctacatattc actatggatt tagtcaatca tttgcccgat cgtttgttat ttgctgttcc
     5461 caaaaagggc agattatatg aaaaatgctg cagcttattg agcggtgccg atatacagtt
     5521 tagaagatct aatagattgg atattgccct ttccacaaac ttgccaattg cattaatctt
     5581 tttgcctgca gccgatattc cagttttcgt tggagaaggt aattgtgact tgggtataac
     5641 tgggttagac cagatcaaag aagcagacca attcgacaac attgaggatt tgttggattt
     5701 gaagtttggc tcatgtaaat tgcagatcca agttcctgca aatggcgagt acgaaaaacc
     5761 cgaacaactc gttggaaaga agattgtatc ctcctttaca aagttgagta ctgactattt
     5821 caagaaatta tccgacaaac ctacgaatat tagatacgtt ggtggttcag ttgaggcttc
     5881 ttgcgcattg ggtgttgccg acgccattgt cgatttagtt gaaagtggag agaccatgaa
     5941 agctgctgga ttaaaggcga tcgaaaccat attggaaact tccgctcatt tgatttcctc
     6001 caagaaaacc aaatttccag aaatggtcaa cataattgtc caaagacttc aaggtgtttt
     6061 agcggctcag gaatatgtct tgtgtaacta caatgctcca aaatccattc aatcaaagtg
     6121 cttaaccatt actccaggta gaagggcagc cactgtctcc actttggata aacacagtga
     6181 cgacgaagat gactgggttg ccatttcttc catggttaac agaaaggaaa ttggtaatgt
     6241 aatggatgaa ttgaagaaag ctggcgcaac agatatattg gtgctcgaga tatcaaattg
     6301 tagagtttaa taaaatacta tatatacata cttatagatt tacttcaatt tgactcaatc
     6361 tgtcttatac catctttatc taccaccttg atgtacacac ctttaaaatc aatgggcatt
     6421 ctcgtttgta attctttgac acacatgtcc attaacttca aaccatcttc aactgtcatg
     6481 tcctttctat aatgtttatc taacaaggat gttgtgtaaa atgctgcgta gccatgagca
     6541 ccataaggac gttcaacttg cgtacctaaa taatctatcc aattcaacga cggctgattt
     6601 gtctttacat cgtatcctcc aattaaacag ttcacctggt acggttttct agaagggcga
     6661 attctgcaga tatccatcac actggtataa cttcgtatag catacattat acgaagttat
     6721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6781 cctaactaga tagcagtcag tcagtcaggc tcatgttcgt gctgtcacag gattacctat
     6841 gccaaagggg ttcactgaat tctccacttc catcaccaat gctattatgt tgaatgtttt
     6901 gggtgataag gcaactccaa ataaagaatt ggagatttgt cgccgtgctt tggaaactcc
     6961 acatgcgtca gtgtatcttt atggtaaaac tactagacca gagcgaaaga tgggtcatat
     7021 caatgttgtc acatcttcca tgcaagatgc cgagagtaga ttaagttata tattgggtga
     7081 taccactgaa attcctaaat ctttagctac cgacaaggaa tctccattgg tgggaataat
     7141 aatgggttcc gattcggatc taccagttat ggcagttggt gctcgtattt tgaaacaatt
     7201 tggtgtgcca ttt
//
