LOCUS       V008259                 7132 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008259
VERSION     V008259
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7132)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7132)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(28..885)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(886..990)
                     /label="AmpR promoter"
     misc_feature    1092..1487
                     /note="5' region of ADE2 genes of Candida albicans; 5' of
                     ADE2"
     regulatory      1503..2975
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     regulatory      2989..3295
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     protein_bind    3332..3365
                     /label="Cre recombinase binding site"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT); loxP"
     CDS             4262..5380
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(5633..5666)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5755..6287
                     /note="3' region of ADE2 genes of Candida albicans; 3' of
                     ADE2"
     rep_origin      complement(6398..6986)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
       61 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
      121 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
      181 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
      241 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      301 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      361 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      421 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      481 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
      541 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
      601 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
      661 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
      721 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
      781 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      841 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
      901 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      961 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     1021 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccagg
     1081 tcgactctag aaggtggcca attaggtcgt atgattgttg aagcagcaca tagattgaat
     1141 atcaagaccg taattttgga cgcagccaaa tcaccagcta aacagatcaa tgctctagac
     1201 gatcacgttg atggatcgtt cacaaactat gattcgattg tgaaattagc tgaaaaggca
     1261 gacgtattaa ctgttgaaat cgaacatgtt gatgttgatg ctttaatcaa agttcaagag
     1321 aaattcccta aagttgaaat atatccatta cctgaaacca taagattgat tcaagataaa
     1381 tatttacaga aaaatcattt aatcaaacac gatgttgcag ttacagaatc tgttgcagtt
     1441 gaaaccaaca cagtcgatga cttgttacac attggggaaa agtttggcga tccgtctagg
     1501 attgaacgat gatattaagc gagaattcac atttttctag aggaatcaca gttacaattg
     1561 tagaggaaac cgaactagta aaactagcac ccaacacttc taaacaaacc aatcaattca
     1621 atggtataac aaatcgtcta ccagaagtta tgctaatatg aaacatcaat tgacaacatg
     1681 gacatcaact gaagctttaa caaatgaata aaaaaaaaag cccatacaat agaccattgg
     1741 tcatagtttc atttgccccc gccccacttt tattgttgag ttgaataatt gtgagcatca
     1801 gctgatttta aaattataaa tttttttttt cgtccattgt taattctatt ttagtttttt
     1861 cttctccatc gtctccgaat tattccggaa gctggtagcg gataagtttt agaatcattg
     1921 agcctctttt caatgccaga tgttatctgc aactgatggt tagttagtta gtataagact
     1981 taaattctca tataggtcat gtgcacggac aagctcattg agtgacgaaa agtcaaattt
     2041 aaacttctct aaacaaattt cagacaatag tccgaaaaaa aaaaacaaaa cccattattg
     2101 tcgacatgtc aaaggattca acggttgaga tggagccgtc aaatatccaa aacactcttc
     2161 tttcaactaa tttgccgtaa actatctcca tcgtacgatt aagataaaaa aaaacaatta
     2221 tttcttacaa tatttgatag agacccaatg caaagccagc gagaaagagg agtattggca
     2281 ttgttgggta gtagatgaat gagtaatctt ctagccaaaa caaaataaac cacaagaaca
     2341 gtaggcaaaa ctgagtactt gatagcgctt gtagtctttg tatttaaaaa aacctaattg
     2401 tccaaaaatt gaaaatcatg agttgcaaat ttgttgaaat caccaggtaa atagatcatt
     2461 tagctatatc gccttatcat tcttcgtaca agaattatac acaaatacca ccaccacacc
     2521 acccagaaat tttacacgtg ttttctaaat tattactttc cgtggccaat caaattgaaa
     2581 tttttcttgt tcgaaacggc agtgcctcaa acattatagt agcaagtatt gcctaaagag
     2641 tttccgtctc gtgccccccc cctatcccct tttgtaattt gccaaaatag ctcaaaaagg
     2701 ataatcccgc actttttttt tccgagttct cgtcatgcaa gcaagcttat tcagaatttt
     2761 cagaggtgcg agtctcacca ctaccactgc agctgcatct tttacagcaa cagcaacagc
     2821 aggtgccacc acggcaaaga cattgtctgg atccactgtg ctccgaaaac tgtataaaag
     2881 gacctattca tccctggtct tatcttctcc agaattattt ttttttcatc agtttaacaa
     2941 caacaaacgt tattgtcata caacaacaac aacaaccgtg catcgctacc attgttcacc
     3001 acaaatgttt ctaagagtga aattctggaa atctggaaat ctggttttgt attcttgtta
     3061 ttcttctttt tgttattaca tatataactt gttacttttt taaaaaaatc tttgtttatt
     3121 ttataaatat ataaaactaa atttaagaaa aagagaaaaa tgttttattt gagagattga
     3181 aattttactt gaatttagct tagcttttat aaagtattat tatgtaaaaa aacaaaacaa
     3241 atatacatta aaaagttaag actataaaat agccacccaa ggcatttcta tatctacagt
     3301 ctgccagact tgccacctga cgtctaagaa aataacttcg tatagcatac attatacgaa
     3361 gttatagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc cttggatcca
     3421 atcatcactg gtgggggtca agaaagaggt ttgagatcag gtactttggc accaccatta
     3481 gtagctgggt ttggtgaagc tgcaagatta atgaaacaag aatctgcatt tgacaagaaa
     3541 catattgaga ggttatctac taaattgaag aacggtttgt tatccattcc atctactcag
     3601 tttaacggtt gtaataaccc aacatatcaa tacccaggtt gtgtgaatgt ttcctttgca
     3661 tacattgaag gggaatcttt gttaatggca ttgaaagata ttgcattgag ttctggttct
     3721 gcatgtacct ctgcatcttt ggaaccatcg tatgttttac atgctttggg tgccgatgat
     3781 gccttggctc attcttccat tagattcggt attggtagat tcaccactga agcagaagtt
     3841 gactatgtta ttcaagcaat taatgaaaga gttgatttct tgagaaagat gtctccatta
     3901 tgggaaatgg ttcaagaagg tattgacttg aactctatcg aatggagtgg acattaaggt
     3961 tttttgtttt tttgtaattt gtgttgttat gtgtaattat cgtgtataga gtaatttcgg
     4021 aaattgtact ttcgttcatg tatctgaacc agatgaataa tacgttcgta ttgttgaaga
     4081 atgactaact cctgtaacgg cgtgcgtggg ttccgcagcc gggttcaatt ttttttttct
     4141 tgttcactat aaaaatatat aataaacgaa aaaaatccga gggactcagg ctatgaaatt
     4201 tttcactcaa gaattttttt tccttcatcc ttaattcctt tattcactac ccaaacccac
     4261 aatgtcagtt aaaactaaaa ctattacaat tttaccaggt gatcacgtcg gtaccgaaat
     4321 tgtcaatgaa gctatcaaag ttttagaagc aattgaagct gccactcctt accaaaaaat
     4381 ccatttcgat ttcaaacatc atttaatcgg tggtgctgcc attgatgcca ctggtgtccc
     4441 attgccagat gacgcacttg aaagtgccaa aaaatctgat gccgtcttat tgggtgctgt
     4501 tggtggacca aaatggggga ctggcgccgt tcgtccagaa caaggtttat taaagattcg
     4561 taaagaattg aacctttatg ctaacatcag accatgtaac tttgccagtg actccttatt
     4621 ggaattatcc ccattgagac cagaagtcgt taaaggtaca aacttgatca ttgttcgtga
     4681 attagttggt ggtatttatt tcggtgatcg tgaagaacaa gaagaaagtg aagataaaca
     4741 aaccgcatgg gatactgaaa aatacaccgt cgatgaagtc accagaatca cccgtatggc
     4801 tgccttcatg gctttacaac ataacccacc attaccaatc tggtcattgg ataaggctaa
     4861 tgttttagct tcttctagat tatggagaag aactgtcgat aaagtgattt ctgaagagtt
     4921 cccaactttg tctgttcaac atcaattgat tgactccgcc gccatgattt taattcaaaa
     4981 cccaaccaaa ttgaatggta taatcatcac ttctaacatg tttggtgata tcatttccga
     5041 tgaagcttca gttattccag gttccttggg tttattacca tctgcatctt tggcttcatt
     5101 gccagatacc aacaccgctt ttggtcttta tgaaccatgt cacggttctg ctcctgattt
     5161 accagccaat aaagttaacc caattgctac tattttatct gctgcttcta tgttgagatt
     5221 atctttggat tgtgtgaaag aagctgaagc tttagaagaa gccgtgaaac aagtcttgga
     5281 taaaggtatc agaactgcag atttaagagg taccagttca gctactgaag ttggtgacgc
     5341 gattgtcgaa gctgttacta aaatcttaaa agaaaaagct taagtaaagt atacacaaaa
     5401 tttaacgaat attattaata caaaatgaac accacctaaa tacttttttt taaaaatgta
     5461 ccgactcttc taaaaattgc gataattcaa tatcggaaat ttctctcacc ggtttacttg
     5521 gatcttcgga aataaatcta tttgaagtaa tggtagcctc acttgattct gattggctct
     5581 ttctagacat gggtaggtaa gggcgaattc tgcagatatc catcacactg gtataacttc
     5641 gtatagcata cattatacga agttatggta atacggttat ccacagaatc aggggataac
     5701 gcaggaaaga acatgtgagc aaaaggccta actagatagc agtcagtcag tcaggctcat
     5761 gttcgtgctg tcacaggatt acctatgcca aaggggttca ctgaattctc cacttccatc
     5821 accaatgcta ttatgttgaa tgttttgggt gataaggcaa ctccaaataa agaattggag
     5881 atttgtcgcc gtgctttgga aactccacat gcgtcagtgt atctttatgg taaaactact
     5941 agaccagagc gaaagatggg tcatatcaat gttgtcacat cttccatgca agatgccgag
     6001 agtagattaa gttatatatt gggtgatacc actgaaattc ctaaatcttt agctaccgac
     6061 aaggaatctc cattggtggg aataataatg ggttccgatt cggatctacc agttatggca
     6121 gttggtgctc gtattttgaa acaatttggt gtgccatttg aactcacaat cgtaagtgca
     6181 cacagaactc ctcatagaat gtctgagtat gctattgaag caccaaagcg tggcttgaaa
     6241 tgtatcattg ctggtgcagg tggtgctgct catttgccag gtatggtgta atcatggtca
     6301 taccgcggag gcctgggccc gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6361 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6421 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6481 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6601 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6661 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6721 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6781 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6841 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6901 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6961 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7021 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7081 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt at
//
