LOCUS       40924_12945        7454 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC160, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7454)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7454)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(673..777)
                     /label=AmpR promoter
     protein_bind    complement(4143..4176)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5702..6415
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="ISKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     rep_origin      complement(6507..7095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7269..7454,1..672))
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
       61 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
      121 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
      181 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
      241 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
      301 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
      361 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
      421 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
      481 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
      541 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
      601 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
      661 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      721 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      781 atttccccga aaagtgccac ctgacgtcta agaaaataac ttcgtatagc atacattata
      841 cgaagttata gctcggatcc actagtaacg gccgccagtg tgctggaatt cgcccttaag
      901 aacatttctg taccgcaaat gtatcgactt tttcgatggt gtgttattgt tgttttgttg
      961 cgaaatataa tagcttctgc tttatatcct ttgtgttgat ccttcccctc ctttcatact
     1021 tgtaaaatta caattggtat tttcaaccaa tattaccaca cgtcatgcac taactcaaat
     1081 gggcaaaaaa aagagagata ggaattacgt cattttatta tgtttttacg tggactagaa
     1141 ggttaagata atttctggtt attttgttga taattgagtt ttgttcgacc tccaccaatc
     1201 tttaaactct cgttgagtat tcattttaat gattttttga aatcagaagg ggtgtttcta
     1261 acccaggatt gttttattga gaggttgaaa catcaatgtg ttagtttatt gatttgggtt
     1321 ggtttaatag aaaaatatca gtaggtgttc aataattgta gtttattttc ttggctaaaa
     1381 ttgttgcgaa aaattgacaa aataataaca aaaagtattt tagatgctaa aacataatag
     1441 taggaatatc tacctgaacc atttacattc aacaactggc aagaaccttc ctaataattt
     1501 gcaagagtaa tcacaattaa accaaaatca aagtttaccg tgtatatagt tggacagaaa
     1561 gctgtcacat tggtttaata catagttgaa tatgtatgca tgaaacaaca acaatacaat
     1621 tacagctcat gatccttcaa ttaatttcaa cattcaaaca acataacacc ttgtgtaaat
     1681 gaggttatga gtacatgtga acaacaaatc ttatgactaa actcgggtcg tgtacttttg
     1741 acgattgagt catttttacc aattaggcaa ttaatttttg ctaaattttc attaaccgcg
     1801 ctggccatcc caacaaacaa tataaaagct gacaacgaaa aaaaaaaaaa aaaaaaaaaa
     1861 actccaattc tgaaaaattt ttcttcttct tcatactaaa caaaacaact ataaagataa
     1921 tcaattaata atgtcacaac aacctagtga aaataaatta tggggtggtc gattcactgg
     1981 cgccactgat ccattaatgg atttatacaa tgcttcatta ccttatgata aagttatgta
     2041 tgatgcagat ttaactggta ctaaagtata tactcaagga ttgaacaaat taggattaat
     2101 taccactgaa gaattgaatc tgattcatca aggtttagaa caaattagac aagaatggca
     2161 tgataataaa tttattatta aagctggtga tgaagatatt catactgcta atgaacgtag
     2221 attaggagaa atcattggga aaaacatttc tggtaaagtt catactggta gatccagaaa
     2281 tgatcaagtt gctactgata tgagaatttt tgttcgtgaa tcattactta atttaagtaa
     2341 gattttacat caattcatta cagcaattct tgaacgagct cataaagaaa ttgatgtatt
     2401 aatgcctggt tatactcatt tacaaaaagc tcaacctatt agatgggctc attggttaag
     2461 ttcatatgca acttatttca ctgaagatta taaacgttta caagaaatca ttactcgtgt
     2521 taatcaatct ccattaggat ctggtgcttt agctggtcat ccctacggta ttgatcgtga
     2581 atttttagct aaaggattag gatttgatgg tgttattggg aattctttaa ctgcagtttc
     2641 tgatcgtgat tttgttgttg aaagtttatt ttggagtaca ttatttatga atcatatttc
     2701 tcgattttca gaagatttaa ttatttattc tagtggtgaa tttggattta taaagttagc
     2761 tgatgcttac tctactggtt catcattaat gccacaaaag aaaaatcctg attcattaga
     2821 attattacgt gggaaaagtg gcagagtgtt tggtcagtta tctgggtttt taatgtcaat
     2881 taaatccatt ccatcaacct ataataaaga tatgcaagaa gataaagaac cattatttga
     2941 tgcattgact accgtagaac attcaatttt aattgctact ggtgtgattt ctactttact
     3001 gattgataaa caaaatatgg aaaaagcttt aactatggat atgttagcta ctgatttagc
     3061 agattattta gttagaaaag gtgttccatt tagagaaact catcatattt ctggtgaatg
     3121 tgttagaaaa gctgaagaag aaaaattgtc gggaattgac caattatcat ttgaacaatt
     3181 tcaacaaatt gattcacgtt ttgaaaaaga tgttatggaa acttttgatt ttgaagctag
     3241 tgtggaaaga agagatgcta ttggtggtac tgctaagagc gctgttttaa aacagttaga
     3301 aaatttgaaa tcaattttaa attaatttat aaataatcat atagtattca atgttttgtt
     3361 ttgttttttt tttttttttt atggaaatgt acaaacagta tataatcaat taatagaaag
     3421 ataccctgta ttccattgta tcgcctttgg gatgtctata ataccaactt tcaagaatga
     3481 gaaattaaac aacaatttct ttcaccaatc aactcgtcta tattcaataa actcattctg
     3541 gtcttttcaa atatttaatg ttatcctgat caaacttaca aatcaaaaat gtatcaaaat
     3601 cattcattag ttcttcttct tttgttatct ttgtcactat atttctctcc atccagacca
     3661 aaaatacttc cattgtgaag atcgtctgaa tcataatcag caactacctc ttccatttca
     3721 gagaattcag atttttcaaa ataggtatca cgggccaagt acacacaaga caaacgatac
     3781 gagtgctggg atatttcttt caatgtatca ctaaattcgt tgagatttaa agagagccct
     3841 ttgactgctt tcattacttt cctcacatca tgagcatcca cattcaaatt tcctgtagcc
     3901 ctttattctt agttttcttt tcttattctt ttcttcgtac attgaaatca caaatggtga
     3961 tatcaaacga aggtcacact gacttatgtc taatacctcc aatgatgata ctggtaactt
     4021 aaccaggtct actcttttca aacgatttct cattagtgtc aatttacgta ggttcttcaa
     4081 cctttcaaac gatgcaaata gcttttgtaa gggcgaattc tgcagatatc catcacactg
     4141 gtataacttc gtatagcata cattatacga agttatggta atacggttat ccacagaatc
     4201 gatccgtcta ggattgaacg atgatattaa gcgagaattc acatttttct agaggaatca
     4261 cagttacaat tgtagaggaa accgaactag taaaactagc acccaacact tctaaacaaa
     4321 ccaatcaatt caatggtata acaaatcgtc taccagaagt tatgctaata tgaaacatca
     4381 attgacaaca tggacatcaa ctgaagcttt aacaaatgaa taaaaaaaaa agcccataca
     4441 atagaccatt ggtcatagtt tcatttgccc ccgccccact tttattgttg agttgaataa
     4501 ttgtgagcat cagctgattt taaaattata aatttttttt ttcgtccatt gttaattcta
     4561 ttttagtttt ttcttctcca tcgtctccga attattccgg aagctggtag cggataagtt
     4621 ttagaatcat tgagcctctt ttcaatgcca gatgttatct gcaactgatg gttagttagt
     4681 tagtataaga cttaaattct catataggtc atgtgcacgg acaagctcat tgagtgacga
     4741 aaagtcaaat ttaaacttct ctaaacaaat ttcagacaat agtccgaaaa aaaaaaacaa
     4801 aacccattat tgtcgacatg tcaaaggatt caacggttga gatggagccg tcaaatatcc
     4861 aaaacactct tctttcaact aatttgccgt aaactatctc catcgtacga ttaagataaa
     4921 aaaaaacaat tatttcttac aatatttgat agagacccaa tgcaaagcca gcgagaaaga
     4981 ggagtattgg cattgttggg tagtagatga atgagtaatc ttctagccaa aacaaaataa
     5041 accacaagaa cagtaggcaa aactgagtac ttgatagcgc ttgtagtctt tgtatttaaa
     5101 aaaacctaat tgtccaaaaa ttgaaaatca tgagttgcaa atttgttgaa atcaccaggt
     5161 aaatagatca tttagctata tcgccttatc attcttcgta caagaattat acacaaatac
     5221 caccaccaca ccacccagaa attttacacg tgttttctaa attattactt tccgtggcca
     5281 atcaaattga aatttttctt gttcgaaacg gcagtgcctc aaacattata gtagcaagta
     5341 ttgcctaaag agtttccgtc tcgtgccccc cccctatccc cttttgtaat ttgccaaaat
     5401 agctcaaaaa ggataatccc gcactttttt tttccgagtt ctcgtcatgc aagcaagctt
     5461 attcagaatt ttcagaggtg cgagtctcac cactaccact gcagctgcat cttttacagc
     5521 aacagcaaca gcaggtgcca ccacggcaaa gacattgtct ggatccactg tgctccgaaa
     5581 actgtataaa aggacctatt catccctggt cttatcttct ccagaattat ttttttttca
     5641 tcagtttaac aacaacaaac gttattgtca tacaacaaca acaacattat tcatgtccac
     5701 aatctctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg
     5761 tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg
     5821 taaattgacc ttaaaattta tttgtactac tggtaaattg ccagttccat ggccaacctt
     5881 agtcactact ttcggttatg gtgttcaatg ttttgcgaga tacccagatc atatgaaaca
     5941 acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt
     6001 caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt
     6061 taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa
     6121 attggaatac aactataact ctcacaatgt ttacatcatg gctgacaaac aaaagaatgg
     6181 tatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggttctgttc aattagctga
     6241 ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta
     6301 cttatccact caatctgcct tatccaaaga tccaaacgaa aagagagacc acatggtctt
     6361 gttagaattt gttactgctg ctggtattac ccatggtatg gatgaattgt acaagtctaa
     6421 gaactcgagg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6481 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6541 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6601 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6661 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6721 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6781 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6841 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6901 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6961 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7021 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7081 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7141 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7201 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7261 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7321 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7381 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7441 ataaaccagc cagc
//
