LOCUS       V008261                 6509 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008261
VERSION     V008261
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6509)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(813..917)
                     /label="AmpR promoter"
     CDS             2065..2958
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(3338..3371)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4897..5610
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     rep_origin      complement(5702..6290)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(join(6464..6509,1..812))
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
       61 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      121 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      181 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      241 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      301 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      361 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      421 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      481 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      541 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      601 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      661 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      721 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      781 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      841 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
      901 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaataac
      961 ttcgtatagc atacattata cgaagttata gctcggatcc actagtaacg gccgccagtg
     1021 tgctggaatt cgcccttgtt tgataccatg gcaagcatac tagaattatt atacgagctg
     1081 ggtgtgaaat gtgccaccgt ttatgcattt tcgatcgaaa acttcaagag actgcaatat
     1141 gaggtcaaat ggttgatgga attagccaaa tccaagttta cgcaaataaa ccaacatgga
     1201 ttgttgtgtg aggagtatgg ggttaaaatc agaatattag gaaataccaa acttttgccg
     1261 aaagatgtgt tagagatcct tgaaagaacc gaagaaataa ctaaaaacaa taagcgtgct
     1321 gtgttgaatg tctgcttccc atatacatca agagacgaga taacccatct gatcaagtca
     1381 gtagtagacg aagcgacgaa aggtggtatt gaaataaccg aggaggctat cgacaagcat
     1441 ctttatacga aagatgtgcc cccattggat ttactagtaa gaacatcagg aacatttaga
     1501 ctttcagact ttcttttatg gcaaacggtg tcgccagatt gtgcagttgt gtttgtcgac
     1561 aagttatggc cggcattcta gaccatggga tatgctcaag attttactca actgggggtt
     1621 taacaaatac atgtatggaa acccaaatgg atatggggtt agtaataact tggtagtgaa
     1681 gagccaaatg gaagaagaat taataaacac accaaatgga gctacagggc ttgaccggta
     1741 cctggaggac gaagagaccg aagttagtag taacaattta ggaactgaag aggatacagt
     1801 gacatccgaa gaagaagatg ttgaagatat aggcaaaaag taataggaca gtattttatt
     1861 tattcaatag tttttttttt ttttttgaaa cgtacacctt gtttatttca ggtgtttctt
     1921 tgaaacgtgt ctcaaagcga taacgggtac aaaaaaaaat gtattgagtc attgaaattt
     1981 ttttattttt tttttttttt ggtgaagatt tttcccacac caaatatata ccacttttct
     2041 tctaacacca cctacatatt cactatggat ttagtcaatc atttgcccga tcgtttgtta
     2101 tttgctgttc ccaaaaaggg cagattatat gaaaaatgct gcagcttatt gagcggtgcc
     2161 gatatacagt ttagaagatc taatagattg gatattgccc tttccacaaa cttgccaatt
     2221 gcattaatct ttttgcctgc agccgatatt ccagttttcg ttggagaagg taattgtgac
     2281 ttgggtataa ctgggttaga ccagatcaaa gaagcagacc aattcgacaa cattgaggat
     2341 ttgttggatt tgaagtttgg ctcatgtaaa ttgcagatcc aagttcctgc aaatggcgag
     2401 tacgaaaaac ccgaacaact cgttggaaag aagattgtat cctcctttac aaagttgagt
     2461 actgactatt tcaagaaatt atccgacaaa cctacgaata ttagatacgt tggtggttca
     2521 gttgaggctt cttgcgcatt gggtgttgcc gacgccattg tcgatttagt tgaaagtgga
     2581 gagaccatga aagctgctgg attaaaggcg atcgaaacca tattggaaac ttccgctcat
     2641 ttgatttcct ccaagaaaac caaatttcca gaaatggtca acataattgt ccaaagactt
     2701 caaggtgttt tagcggctca ggaatatgtc ttgtgtaact acaatgctcc aaaatccatt
     2761 caatcaaagt gcttaaccat tactccaggt agaagggcag ccactgtctc cactttggat
     2821 aaacacagtg acgacgaaga tgactgggtt gccatttctt ccatggttaa cagaaaggaa
     2881 attggtaatg taatggatga attgaagaaa gctggcgcaa cagatatatt ggtgctcgag
     2941 atatcaaatt gtagagttta ataaaatact atatatacat acttatagat ttacttcaat
     3001 ttgactcaat ctgtcttata ccatctttat ctaccacctt gatgtacaca cctttaaaat
     3061 caatgggcat tctcgtttgt aattctttga cacacatgtc cattaacttc aaaccatctt
     3121 caactgtcat gtcctttcta taatgtttat ctaacaagga tgttgtgtaa aatgctgcgt
     3181 agccatgagc accataagga cgttcaactt gcgtacctaa ataatctatc caattcaacg
     3241 acggctgatt tgtctttaca tcgtatcctc caattaaaca gttcacctgg tacggttttc
     3301 tagaagggcg aattctgcag atatccatca cactggtata acttcgtata gcatacatta
     3361 tacgaagtta tggtaatacg gttatccaca gaatcgatcc gtctaggatt gaacgatgat
     3421 attaagcgag aattcacatt tttctagagg aatcacagtt acaattgtag aggaaaccga
     3481 actagtaaaa ctagcaccca acacttctaa acaaaccaat caattcaatg gtataacaaa
     3541 tcgtctacca gaagttatgc taatatgaaa catcaattga caacatggac atcaactgaa
     3601 gctttaacaa atgaataaaa aaaaaagccc atacaataga ccattggtca tagtttcatt
     3661 tgcccccgcc ccacttttat tgttgagttg aataattgtg agcatcagct gattttaaaa
     3721 ttataaattt tttttttcgt ccattgttaa ttctatttta gttttttctt ctccatcgtc
     3781 tccgaattat tccggaagct ggtagcggat aagttttaga atcattgagc ctcttttcaa
     3841 tgccagatgt tatctgcaac tgatggttag ttagttagta taagacttaa attctcatat
     3901 aggtcatgtg cacggacaag ctcattgagt gacgaaaagt caaatttaaa cttctctaaa
     3961 caaatttcag acaatagtcc gaaaaaaaaa aacaaaaccc attattgtcg acatgtcaaa
     4021 ggattcaacg gttgagatgg agccgtcaaa tatccaaaac actcttcttt caactaattt
     4081 gccgtaaact atctccatcg tacgattaag ataaaaaaaa acaattattt cttacaatat
     4141 ttgatagaga cccaatgcaa agccagcgag aaagaggagt attggcattg ttgggtagta
     4201 gatgaatgag taatcttcta gccaaaacaa aataaaccac aagaacagta ggcaaaactg
     4261 agtacttgat agcgcttgta gtctttgtat ttaaaaaaac ctaattgtcc aaaaattgaa
     4321 aatcatgagt tgcaaatttg ttgaaatcac caggtaaata gatcatttag ctatatcgcc
     4381 ttatcattct tcgtacaaga attatacaca aataccacca ccacaccacc cagaaatttt
     4441 acacgtgttt tctaaattat tactttccgt ggccaatcaa attgaaattt ttcttgttcg
     4501 aaacggcagt gcctcaaaca ttatagtagc aagtattgcc taaagagttt ccgtctcgtg
     4561 ccccccccct atcccctttt gtaatttgcc aaaatagctc aaaaaggata atcccgcact
     4621 ttttttttcc gagttctcgt catgcaagca agcttattca gaattttcag aggtgcgagt
     4681 ctcaccacta ccactgcagc tgcatctttt acagcaacag caacagcagg tgccaccacg
     4741 gcaaagacat tgtctggatc cactgtgctc cgaaaactgt ataaaaggac ctattcatcc
     4801 ctggtcttat cttctccaga attatttttt tttcatcagt ttaacaacaa caaacgttat
     4861 tgtcatacaa caacaacaac attattcatg tccacaatct ctaaaggtga agaattattc
     4921 actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct
     4981 gtctccggtg aaggtgaagg tgatgctact tacggtaaat tgaccttaaa atttatttgt
     5041 actactggta aattgccagt tccatggcca accttagtca ctactttcgg ttatggtgtt
     5101 caatgttttg cgagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg
     5161 ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc
     5221 agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt
     5281 gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac
     5341 aatgtttaca tcatggctga caaacaaaag aatggtatca aagttaactt caaaattaga
     5401 cacaacattg aagatggttc tgttcaatta gctgaccatt atcaacaaaa tactccaatt
     5461 ggtgatggtc cagtcttgtt accagacaac cattacttat ccactcaatc tgccttatcc
     5521 aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttgttac tgctgctggt
     5581 attacccatg gtatggatga attgtacaag tctaagaact cgaggataac gcaggaaaga
     5641 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5701 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5761 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5821 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5881 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5941 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6001 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6061 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6121 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6181 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6241 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6301 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6361 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6421 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6481 aggcacctat ctcagcgatc tgtctattt
//
