LOCUS       V008262                 6428 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008262
VERSION     V008262
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6428)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6428)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(375..1232)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1233..1337)
                     /label="AmpR promoter"
     CDS             2306..3424
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(3677..3710)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5236..5949
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     rep_origin      complement(join(6041..6428,1..201))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
       61 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      121 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      181 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      241 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      301 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      361 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      421 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      481 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      541 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      601 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      661 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      721 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      781 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      841 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      901 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      961 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1021 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1081 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1141 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1201 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1261 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1321 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaataac
     1381 ttcgtatagc atacattata cgaagttata gctcggatcc actagtaacg gccgccagtg
     1441 tgctggaatt cgcccttgga tccaatcatc actggtgggg gtcaagaaag aggtttgaga
     1501 tcaggtactt tggcaccacc attagtagct gggtttggtg aagctgcaag attaatgaaa
     1561 caagaatctg catttgacaa gaaacatatt gagaggttat ctactaaatt gaagaacggt
     1621 ttgttatcca ttccatctac tcagtttaac ggttgtaata acccaacata tcaataccca
     1681 ggttgtgtga atgtttcctt tgcatacatt gaaggggaat ctttgttaat ggcattgaaa
     1741 gatattgcat tgagttctgg ttctgcatgt acctctgcat ctttggaacc atcgtatgtt
     1801 ttacatgctt tgggtgccga tgatgccttg gctcattctt ccattagatt cggtattggt
     1861 agattcacca ctgaagcaga agttgactat gttattcaag caattaatga aagagttgat
     1921 ttcttgagaa agatgtctcc attatgggaa atggttcaag aaggtattga cttgaactct
     1981 atcgaatgga gtggacatta aggttttttg tttttttgta atttgtgttg ttatgtgtaa
     2041 ttatcgtgta tagagtaatt tcggaaattg tactttcgtt catgtatctg aaccagatga
     2101 ataatacgtt cgtattgttg aagaatgact aactcctgta acggcgtgcg tgggttccgc
     2161 agccgggttc aatttttttt ttcttgttca ctataaaaat atataataaa cgaaaaaaat
     2221 ccgagggact caggctatga aatttttcac tcaagaattt tttttccttc atccttaatt
     2281 cctttattca ctacccaaac ccacaatgtc agttaaaact aaaactatta caattttacc
     2341 aggtgatcac gtcggtaccg aaattgtcaa tgaagctatc aaagttttag aagcaattga
     2401 agctgccact ccttaccaaa aaatccattt cgatttcaaa catcatttaa tcggtggtgc
     2461 tgccattgat gccactggtg tcccattgcc agatgacgca cttgaaagtg ccaaaaaatc
     2521 tgatgccgtc ttattgggtg ctgttggtgg accaaaatgg gggactggcg ccgttcgtcc
     2581 agaacaaggt ttattaaaga ttcgtaaaga attgaacctt tatgctaaca tcagaccatg
     2641 taactttgcc agtgactcct tattggaatt atccccattg agaccagaag tcgttaaagg
     2701 tacaaacttg atcattgttc gtgaattagt tggtggtatt tatttcggtg atcgtgaaga
     2761 acaagaagaa agtgaagata aacaaaccgc atgggatact gaaaaataca ccgtcgatga
     2821 agtcaccaga atcacccgta tggctgcctt catggcttta caacataacc caccattacc
     2881 aatctggtca ttggataagg ctaatgtttt agcttcttct agattatgga gaagaactgt
     2941 cgataaagtg atttctgaag agttcccaac tttgtctgtt caacatcaat tgattgactc
     3001 cgccgccatg attttaattc aaaacccaac caaattgaat ggtataatca tcacttctaa
     3061 catgtttggt gatatcattt ccgatgaagc ttcagttatt ccaggttcct tgggtttatt
     3121 accatctgca tctttggctt cattgccaga taccaacacc gcttttggtc tttatgaacc
     3181 atgtcacggt tctgctcctg atttaccagc caataaagtt aacccaattg ctactatttt
     3241 atctgctgct tctatgttga gattatcttt ggattgtgtg aaagaagctg aagctttaga
     3301 agaagccgtg aaacaagtct tggataaagg tatcagaact gcagatttaa gaggtaccag
     3361 ttcagctact gaagttggtg acgcgattgt cgaagctgtt actaaaatct taaaagaaaa
     3421 agcttaagta aagtatacac aaaatttaac gaatattatt aatacaaaat gaacaccacc
     3481 taaatacttt tttttaaaaa tgtaccgact cttctaaaaa ttgcgataat tcaatatcgg
     3541 aaatttctct caccggttta cttggatctt cggaaataaa tctatttgaa gtaatggtag
     3601 cctcacttga ttctgattgg ctctttctag acatgggtag gtaagggcga attctgcaga
     3661 tatccatcac actggtataa cttcgtatag catacattat acgaagttat ggtaatacgg
     3721 ttatccacag aatcgatccg tctaggattg aacgatgata ttaagcgaga attcacattt
     3781 ttctagagga atcacagtta caattgtaga ggaaaccgaa ctagtaaaac tagcacccaa
     3841 cacttctaaa caaaccaatc aattcaatgg tataacaaat cgtctaccag aagttatgct
     3901 aatatgaaac atcaattgac aacatggaca tcaactgaag ctttaacaaa tgaataaaaa
     3961 aaaaagccca tacaatagac cattggtcat agtttcattt gcccccgccc cacttttatt
     4021 gttgagttga ataattgtga gcatcagctg attttaaaat tataaatttt ttttttcgtc
     4081 cattgttaat tctattttag ttttttcttc tccatcgtct ccgaattatt ccggaagctg
     4141 gtagcggata agttttagaa tcattgagcc tcttttcaat gccagatgtt atctgcaact
     4201 gatggttagt tagttagtat aagacttaaa ttctcatata ggtcatgtgc acggacaagc
     4261 tcattgagtg acgaaaagtc aaatttaaac ttctctaaac aaatttcaga caatagtccg
     4321 aaaaaaaaaa acaaaaccca ttattgtcga catgtcaaag gattcaacgg ttgagatgga
     4381 gccgtcaaat atccaaaaca ctcttctttc aactaatttg ccgtaaacta tctccatcgt
     4441 acgattaaga taaaaaaaaa caattatttc ttacaatatt tgatagagac ccaatgcaaa
     4501 gccagcgaga aagaggagta ttggcattgt tgggtagtag atgaatgagt aatcttctag
     4561 ccaaaacaaa ataaaccaca agaacagtag gcaaaactga gtacttgata gcgcttgtag
     4621 tctttgtatt taaaaaaacc taattgtcca aaaattgaaa atcatgagtt gcaaatttgt
     4681 tgaaatcacc aggtaaatag atcatttagc tatatcgcct tatcattctt cgtacaagaa
     4741 ttatacacaa ataccaccac cacaccaccc agaaatttta cacgtgtttt ctaaattatt
     4801 actttccgtg gccaatcaaa ttgaaatttt tcttgttcga aacggcagtg cctcaaacat
     4861 tatagtagca agtattgcct aaagagtttc cgtctcgtgc ccccccccta tccccttttg
     4921 taatttgcca aaatagctca aaaaggataa tcccgcactt tttttttccg agttctcgtc
     4981 atgcaagcaa gcttattcag aattttcaga ggtgcgagtc tcaccactac cactgcagct
     5041 gcatctttta cagcaacagc aacagcaggt gccaccacgg caaagacatt gtctggatcc
     5101 actgtgctcc gaaaactgta taaaaggacc tattcatccc tggtcttatc ttctccagaa
     5161 ttattttttt ttcatcagtt taacaacaac aaacgttatt gtcatacaac aacaacaaca
     5221 ttattcatgt ccacaatctc taaaggtgaa gaattattca ctggtgttgt cccaattttg
     5281 gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt
     5341 gatgctactt acggtaaatt gaccttaaaa tttatttgta ctactggtaa attgccagtt
     5401 ccatggccaa ccttagtcac tactttcggt tatggtgttc aatgttttgc gagataccca
     5461 gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa
     5521 agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa
     5581 ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac
     5641 attttaggtc acaaattgga atacaactat aactctcaca atgtttacat catggctgac
     5701 aaacaaaaga atggtatcaa agttaacttc aaaattagac acaacattga agatggttct
     5761 gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta
     5821 ccagacaacc attacttatc cactcaatct gccttatcca aagatccaaa cgaaaagaga
     5881 gaccacatgg tcttgttaga atttgttact gctgctggta ttacccatgg tatggatgaa
     5941 ttgtacaagt ctaagaactc gaggataacg caggaaagaa catgtgagca aaaggccagc
     6001 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6061 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6121 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6181 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6241 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6301 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6361 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6421 ggtatgta
//
