LOCUS       40924_12930        7407 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC157, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7407)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(593..697)
                     /label=AmpR promoter
     protein_bind    complement(4063..4096)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5655..5681
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             5685..5711
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             5715..5741
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     rep_origin      complement(6380..6968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7142..7407,1..592))
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
       61 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
      121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
      181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
      241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
      301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
      361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
      421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
      481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      721 ctgacgtcta agaaaataac ttcgtatagc atacattata cgaagttata gctcggatcc
      781 actagtaacg gccgccagtg tgctggaatt cgcccttaag aacatttctg taccgcaaat
      841 gtatcgactt tttcgatggt gtgttattgt tgttttgttg cgaaatataa tagcttctgc
      901 tttatatcct ttgtgttgat ccttcccctc ctttcatact tgtaaaatta caattggtat
      961 tttcaaccaa tattaccaca cgtcatgcac taactcaaat gggcaaaaaa aagagagata
     1021 ggaattacgt cattttatta tgtttttacg tggactagaa ggttaagata atttctggtt
     1081 attttgttga taattgagtt ttgttcgacc tccaccaatc tttaaactct cgttgagtat
     1141 tcattttaat gattttttga aatcagaagg ggtgtttcta acccaggatt gttttattga
     1201 gaggttgaaa catcaatgtg ttagtttatt gatttgggtt ggtttaatag aaaaatatca
     1261 gtaggtgttc aataattgta gtttattttc ttggctaaaa ttgttgcgaa aaattgacaa
     1321 aataataaca aaaagtattt tagatgctaa aacataatag taggaatatc tacctgaacc
     1381 atttacattc aacaactggc aagaaccttc ctaataattt gcaagagtaa tcacaattaa
     1441 accaaaatca aagtttaccg tgtatatagt tggacagaaa gctgtcacat tggtttaata
     1501 catagttgaa tatgtatgca tgaaacaaca acaatacaat tacagctcat gatccttcaa
     1561 ttaatttcaa cattcaaaca acataacacc ttgtgtaaat gaggttatga gtacatgtga
     1621 acaacaaatc ttatgactaa actcgggtcg tgtacttttg acgattgagt catttttacc
     1681 aattaggcaa ttaatttttg ctaaattttc attaaccgcg ctggccatcc caacaaacaa
     1741 tataaaagct gacaacgaaa aaaaaaaaaa aaaaaaaaaa actccaattc tgaaaaattt
     1801 ttcttcttct tcatactaaa caaaacaact ataaagataa tcaattaata atgtcacaac
     1861 aacctagtga aaataaatta tggggtggtc gattcactgg cgccactgat ccattaatgg
     1921 atttatacaa tgcttcatta ccttatgata aagttatgta tgatgcagat ttaactggta
     1981 ctaaagtata tactcaagga ttgaacaaat taggattaat taccactgaa gaattgaatc
     2041 tgattcatca aggtttagaa caaattagac aagaatggca tgataataaa tttattatta
     2101 aagctggtga tgaagatatt catactgcta atgaacgtag attaggagaa atcattggga
     2161 aaaacatttc tggtaaagtt catactggta gatccagaaa tgatcaagtt gctactgata
     2221 tgagaatttt tgttcgtgaa tcattactta atttaagtaa gattttacat caattcatta
     2281 cagcaattct tgaacgagct cataaagaaa ttgatgtatt aatgcctggt tatactcatt
     2341 tacaaaaagc tcaacctatt agatgggctc attggttaag ttcatatgca acttatttca
     2401 ctgaagatta taaacgttta caagaaatca ttactcgtgt taatcaatct ccattaggat
     2461 ctggtgcttt agctggtcat ccctacggta ttgatcgtga atttttagct aaaggattag
     2521 gatttgatgg tgttattggg aattctttaa ctgcagtttc tgatcgtgat tttgttgttg
     2581 aaagtttatt ttggagtaca ttatttatga atcatatttc tcgattttca gaagatttaa
     2641 ttatttattc tagtggtgaa tttggattta taaagttagc tgatgcttac tctactggtt
     2701 catcattaat gccacaaaag aaaaatcctg attcattaga attattacgt gggaaaagtg
     2761 gcagagtgtt tggtcagtta tctgggtttt taatgtcaat taaatccatt ccatcaacct
     2821 ataataaaga tatgcaagaa gataaagaac cattatttga tgcattgact accgtagaac
     2881 attcaatttt aattgctact ggtgtgattt ctactttact gattgataaa caaaatatgg
     2941 aaaaagcttt aactatggat atgttagcta ctgatttagc agattattta gttagaaaag
     3001 gtgttccatt tagagaaact catcatattt ctggtgaatg tgttagaaaa gctgaagaag
     3061 aaaaattgtc gggaattgac caattatcat ttgaacaatt tcaacaaatt gattcacgtt
     3121 ttgaaaaaga tgttatggaa acttttgatt ttgaagctag tgtggaaaga agagatgcta
     3181 ttggtggtac tgctaagagc gctgttttaa aacagttaga aaatttgaaa tcaattttaa
     3241 attaatttat aaataatcat atagtattca atgttttgtt ttgttttttt tttttttttt
     3301 atggaaatgt acaaacagta tataatcaat taatagaaag ataccctgta ttccattgta
     3361 tcgcctttgg gatgtctata ataccaactt tcaagaatga gaaattaaac aacaatttct
     3421 ttcaccaatc aactcgtcta tattcaataa actcattctg gtcttttcaa atatttaatg
     3481 ttatcctgat caaacttaca aatcaaaaat gtatcaaaat cattcattag ttcttcttct
     3541 tttgttatct ttgtcactat atttctctcc atccagacca aaaatacttc cattgtgaag
     3601 atcgtctgaa tcataatcag caactacctc ttccatttca gagaattcag atttttcaaa
     3661 ataggtatca cgggccaagt acacacaaga caaacgatac gagtgctggg atatttcttt
     3721 caatgtatca ctaaattcgt tgagatttaa agagagccct ttgactgctt tcattacttt
     3781 cctcacatca tgagcatcca cattcaaatt tcctgtagcc ctttattctt agttttcttt
     3841 tcttattctt ttcttcgtac attgaaatca caaatggtga tatcaaacga aggtcacact
     3901 gacttatgtc taatacctcc aatgatgata ctggtaactt aaccaggtct actcttttca
     3961 aacgatttct cattagtgtc aatttacgta ggttcttcaa cctttcaaac gatgcaaata
     4021 gcttttgtaa gggcgaattc tgcagatatc catcacactg gtataacttc gtatagcata
     4081 cattatacga agttatggta atacggttat ccacagaatc gatccgtcta ggattgaacg
     4141 atgatattaa gcgagaattc acatttttct agaggaatca cagttacaat tgtagaggaa
     4201 accgaactag taaaactagc acccaacact tctaaacaaa ccaatcaatt caatggtata
     4261 acaaatcgtc taccagaagt tatgctaata tgaaacatca attgacaaca tggacatcaa
     4321 ctgaagcttt aacaaatgaa taaaaaaaaa agcccataca atagaccatt ggtcatagtt
     4381 tcatttgccc ccgccccact tttattgttg agttgaataa ttgtgagcat cagctgattt
     4441 taaaattata aatttttttt ttcgtccatt gttaattcta ttttagtttt ttcttctcca
     4501 tcgtctccga attattccgg aagctggtag cggataagtt ttagaatcat tgagcctctt
     4561 ttcaatgcca gatgttatct gcaactgatg gttagttagt tagtataaga cttaaattct
     4621 catataggtc atgtgcacgg acaagctcat tgagtgacga aaagtcaaat ttaaacttct
     4681 ctaaacaaat ttcagacaat agtccgaaaa aaaaaaacaa aacccattat tgtcgacatg
     4741 tcaaaggatt caacggttga gatggagccg tcaaatatcc aaaacactct tctttcaact
     4801 aatttgccgt aaactatctc catcgtacga ttaagataaa aaaaaacaat tatttcttac
     4861 aatatttgat agagacccaa tgcaaagcca gcgagaaaga ggagtattgg cattgttggg
     4921 tagtagatga atgagtaatc ttctagccaa aacaaaataa accacaagaa cagtaggcaa
     4981 aactgagtac ttgatagcgc ttgtagtctt tgtatttaaa aaaacctaat tgtccaaaaa
     5041 ttgaaaatca tgagttgcaa atttgttgaa atcaccaggt aaatagatca tttagctata
     5101 tcgccttatc attcttcgta caagaattat acacaaatac caccaccaca ccacccagaa
     5161 attttacacg tgttttctaa attattactt tccgtggcca atcaaattga aatttttctt
     5221 gttcgaaacg gcagtgcctc aaacattata gtagcaagta ttgcctaaag agtttccgtc
     5281 tcgtgccccc cccctatccc cttttgtaat ttgccaaaat agctcaaaaa ggataatccc
     5341 gcactttttt tttccgagtt ctcgtcatgc aagcaagctt attcagaatt ttcagaggtg
     5401 cgagtctcac cactaccact gcagctgcat cttttacagc aacagcaaca gcaggtgcca
     5461 ccacggcaaa gacattgtct ggatccactg tgctccgaaa actgtataaa aggacctatt
     5521 catccctggt cttatcttct ccagaattat ttttttttca tcagtttaac aacaacaaac
     5581 gttattgtca tacaacaaca acaacattat tcatgtccac acgcaagctc gcgagcgcgc
     5641 caattagatc cgtgtaccca tacgatgttc cagattacgc tggttaccca tacgatgttc
     5701 cagattacgc tggttaccca tacgatgttc cagattacgc tatcgatatc ccatggagat
     5761 ctacgctagg atcgatatct cgagacgcgt gatcttggac accagtaaat aagcaaatag
     5821 ctaaatcata tacgaattaa tgttatgatt aagtgtttac gtgagtgcga tatttttatt
     5881 actatcttat acagttgtat atactctata aaatgagttg tctattaatt aacgatgaat
     5941 gctttctggg tttacctctc caacgactct agtttacttc tcaatacatt caattgtatt
     6001 tgatttgtca atacttcatc attaatcaat tctatagttt tgtttttctc gtttatttcc
     6061 aaatttaatg catcaatttt attattcaat ttgtcgttga ttttggttaa tgattttatg
     6121 gtttgatctc tggcattgat tgtttgtgtt agtttttcat tattgataat taaactattt
     6181 aagttagtta tcaactcggt gttttcaagt tttaagtttt caatttcttt agagtttata
     6241 agatttgtca aagtttctga attgcttgat tggtcctgta gaagcgtgtc taagaactcg
     6301 aggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6361 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6421 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6481 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6541 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6601 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6661 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6721 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6781 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6841 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6901 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6961 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7021 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7081 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7141 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7201 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7261 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7321 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7381 ctattaattg ttgccgggaa gctagag
//
