LOCUS       V008264                 6462 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008264
VERSION     V008264
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6462)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6462)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(255..1112)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1113..1217)
                     /label="AmpR promoter"
     CDS             2365..3258
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(3638..3671)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5230..5256
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             5260..5286
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             5290..5316
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     rep_origin      complement(join(5955..6462,1..81))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
       61 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      121 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      181 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      241 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      301 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      361 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      421 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      481 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      541 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      601 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      661 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      721 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      781 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      841 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      901 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      961 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1021 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1081 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1141 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1201 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaataac
     1261 ttcgtatagc atacattata cgaagttata gctcggatcc actagtaacg gccgccagtg
     1321 tgctggaatt cgcccttgtt tgataccatg gcaagcatac tagaattatt atacgagctg
     1381 ggtgtgaaat gtgccaccgt ttatgcattt tcgatcgaaa acttcaagag actgcaatat
     1441 gaggtcaaat ggttgatgga attagccaaa tccaagttta cgcaaataaa ccaacatgga
     1501 ttgttgtgtg aggagtatgg ggttaaaatc agaatattag gaaataccaa acttttgccg
     1561 aaagatgtgt tagagatcct tgaaagaacc gaagaaataa ctaaaaacaa taagcgtgct
     1621 gtgttgaatg tctgcttccc atatacatca agagacgaga taacccatct gatcaagtca
     1681 gtagtagacg aagcgacgaa aggtggtatt gaaataaccg aggaggctat cgacaagcat
     1741 ctttatacga aagatgtgcc cccattggat ttactagtaa gaacatcagg aacatttaga
     1801 ctttcagact ttcttttatg gcaaacggtg tcgccagatt gtgcagttgt gtttgtcgac
     1861 aagttatggc cggcattcta gaccatggga tatgctcaag attttactca actgggggtt
     1921 taacaaatac atgtatggaa acccaaatgg atatggggtt agtaataact tggtagtgaa
     1981 gagccaaatg gaagaagaat taataaacac accaaatgga gctacagggc ttgaccggta
     2041 cctggaggac gaagagaccg aagttagtag taacaattta ggaactgaag aggatacagt
     2101 gacatccgaa gaagaagatg ttgaagatat aggcaaaaag taataggaca gtattttatt
     2161 tattcaatag tttttttttt ttttttgaaa cgtacacctt gtttatttca ggtgtttctt
     2221 tgaaacgtgt ctcaaagcga taacgggtac aaaaaaaaat gtattgagtc attgaaattt
     2281 ttttattttt tttttttttt ggtgaagatt tttcccacac caaatatata ccacttttct
     2341 tctaacacca cctacatatt cactatggat ttagtcaatc atttgcccga tcgtttgtta
     2401 tttgctgttc ccaaaaaggg cagattatat gaaaaatgct gcagcttatt gagcggtgcc
     2461 gatatacagt ttagaagatc taatagattg gatattgccc tttccacaaa cttgccaatt
     2521 gcattaatct ttttgcctgc agccgatatt ccagttttcg ttggagaagg taattgtgac
     2581 ttgggtataa ctgggttaga ccagatcaaa gaagcagacc aattcgacaa cattgaggat
     2641 ttgttggatt tgaagtttgg ctcatgtaaa ttgcagatcc aagttcctgc aaatggcgag
     2701 tacgaaaaac ccgaacaact cgttggaaag aagattgtat cctcctttac aaagttgagt
     2761 actgactatt tcaagaaatt atccgacaaa cctacgaata ttagatacgt tggtggttca
     2821 gttgaggctt cttgcgcatt gggtgttgcc gacgccattg tcgatttagt tgaaagtgga
     2881 gagaccatga aagctgctgg attaaaggcg atcgaaacca tattggaaac ttccgctcat
     2941 ttgatttcct ccaagaaaac caaatttcca gaaatggtca acataattgt ccaaagactt
     3001 caaggtgttt tagcggctca ggaatatgtc ttgtgtaact acaatgctcc aaaatccatt
     3061 caatcaaagt gcttaaccat tactccaggt agaagggcag ccactgtctc cactttggat
     3121 aaacacagtg acgacgaaga tgactgggtt gccatttctt ccatggttaa cagaaaggaa
     3181 attggtaatg taatggatga attgaagaaa gctggcgcaa cagatatatt ggtgctcgag
     3241 atatcaaatt gtagagttta ataaaatact atatatacat acttatagat ttacttcaat
     3301 ttgactcaat ctgtcttata ccatctttat ctaccacctt gatgtacaca cctttaaaat
     3361 caatgggcat tctcgtttgt aattctttga cacacatgtc cattaacttc aaaccatctt
     3421 caactgtcat gtcctttcta taatgtttat ctaacaagga tgttgtgtaa aatgctgcgt
     3481 agccatgagc accataagga cgttcaactt gcgtacctaa ataatctatc caattcaacg
     3541 acggctgatt tgtctttaca tcgtatcctc caattaaaca gttcacctgg tacggttttc
     3601 tagaagggcg aattctgcag atatccatca cactggtata acttcgtata gcatacatta
     3661 tacgaagtta tggtaatacg gttatccaca gaatcgatcc gtctaggatt gaacgatgat
     3721 attaagcgag aattcacatt tttctagagg aatcacagtt acaattgtag aggaaaccga
     3781 actagtaaaa ctagcaccca acacttctaa acaaaccaat caattcaatg gtataacaaa
     3841 tcgtctacca gaagttatgc taatatgaaa catcaattga caacatggac atcaactgaa
     3901 gctttaacaa atgaataaaa aaaaaagccc atacaataga ccattggtca tagtttcatt
     3961 tgcccccgcc ccacttttat tgttgagttg aataattgtg agcatcagct gattttaaaa
     4021 ttataaattt tttttttcgt ccattgttaa ttctatttta gttttttctt ctccatcgtc
     4081 tccgaattat tccggaagct ggtagcggat aagttttaga atcattgagc ctcttttcaa
     4141 tgccagatgt tatctgcaac tgatggttag ttagttagta taagacttaa attctcatat
     4201 aggtcatgtg cacggacaag ctcattgagt gacgaaaagt caaatttaaa cttctctaaa
     4261 caaatttcag acaatagtcc gaaaaaaaaa aacaaaaccc attattgtcg acatgtcaaa
     4321 ggattcaacg gttgagatgg agccgtcaaa tatccaaaac actcttcttt caactaattt
     4381 gccgtaaact atctccatcg tacgattaag ataaaaaaaa acaattattt cttacaatat
     4441 ttgatagaga cccaatgcaa agccagcgag aaagaggagt attggcattg ttgggtagta
     4501 gatgaatgag taatcttcta gccaaaacaa aataaaccac aagaacagta ggcaaaactg
     4561 agtacttgat agcgcttgta gtctttgtat ttaaaaaaac ctaattgtcc aaaaattgaa
     4621 aatcatgagt tgcaaatttg ttgaaatcac caggtaaata gatcatttag ctatatcgcc
     4681 ttatcattct tcgtacaaga attatacaca aataccacca ccacaccacc cagaaatttt
     4741 acacgtgttt tctaaattat tactttccgt ggccaatcaa attgaaattt ttcttgttcg
     4801 aaacggcagt gcctcaaaca ttatagtagc aagtattgcc taaagagttt ccgtctcgtg
     4861 ccccccccct atcccctttt gtaatttgcc aaaatagctc aaaaaggata atcccgcact
     4921 ttttttttcc gagttctcgt catgcaagca agcttattca gaattttcag aggtgcgagt
     4981 ctcaccacta ccactgcagc tgcatctttt acagcaacag caacagcagg tgccaccacg
     5041 gcaaagacat tgtctggatc cactgtgctc cgaaaactgt ataaaaggac ctattcatcc
     5101 ctggtcttat cttctccaga attatttttt tttcatcagt ttaacaacaa caaacgttat
     5161 tgtcatacaa caacaacaac attattcatg tccacacgca agctcgcgag cgcgccaatt
     5221 agatccgtgt acccatacga tgttccagat tacgctggtt acccatacga tgttccagat
     5281 tacgctggtt acccatacga tgttccagat tacgctatcg atatcccatg gagatctacg
     5341 ctaggatcga tatctcgaga cgcgtgatct tggacaccag taaataagca aatagctaaa
     5401 tcatatacga attaatgtta tgattaagtg tttacgtgag tgcgatattt ttattactat
     5461 cttatacagt tgtatatact ctataaaatg agttgtctat taattaacga tgaatgcttt
     5521 ctgggtttac ctctccaacg actctagttt acttctcaat acattcaatt gtatttgatt
     5581 tgtcaatact tcatcattaa tcaattctat agttttgttt ttctcgttta tttccaaatt
     5641 taatgcatca attttattat tcaatttgtc gttgattttg gttaatgatt ttatggtttg
     5701 atctctggca ttgattgttt gtgttagttt ttcattattg ataattaaac tatttaagtt
     5761 agttatcaac tcggtgtttt caagttttaa gttttcaatt tctttagagt ttataagatt
     5821 tgtcaaagtt tctgaattgc ttgattggtc ctgtagaagc gtgtctaaga actcgaggat
     5881 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5941 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6001 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6061 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6121 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6181 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6241 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6301 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6361 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6421 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt ga
//
