LOCUS       40924_12915        7303 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC154, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7303)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7303)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(593..697)
                     /label=AmpR promoter
     protein_bind    complement(4063..4096)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5682..5711
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     rep_origin      complement(6276..6864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7038..7303,1..592))
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
       61 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
      121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
      181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
      241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
      301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
      361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
      421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
      481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      721 ctgacgtcta agaaaataac ttcgtatagc atacattata cgaagttata gctcggatcc
      781 actagtaacg gccgccagtg tgctggaatt cgcccttaag aacatttctg taccgcaaat
      841 gtatcgactt tttcgatggt gtgttattgt tgttttgttg cgaaatataa tagcttctgc
      901 tttatatcct ttgtgttgat ccttcccctc ctttcatact tgtaaaatta caattggtat
      961 tttcaaccaa tattaccaca cgtcatgcac taactcaaat gggcaaaaaa aagagagata
     1021 ggaattacgt cattttatta tgtttttacg tggactagaa ggttaagata atttctggtt
     1081 attttgttga taattgagtt ttgttcgacc tccaccaatc tttaaactct cgttgagtat
     1141 tcattttaat gattttttga aatcagaagg ggtgtttcta acccaggatt gttttattga
     1201 gaggttgaaa catcaatgtg ttagtttatt gatttgggtt ggtttaatag aaaaatatca
     1261 gtaggtgttc aataattgta gtttattttc ttggctaaaa ttgttgcgaa aaattgacaa
     1321 aataataaca aaaagtattt tagatgctaa aacataatag taggaatatc tacctgaacc
     1381 atttacattc aacaactggc aagaaccttc ctaataattt gcaagagtaa tcacaattaa
     1441 accaaaatca aagtttaccg tgtatatagt tggacagaaa gctgtcacat tggtttaata
     1501 catagttgaa tatgtatgca tgaaacaaca acaatacaat tacagctcat gatccttcaa
     1561 ttaatttcaa cattcaaaca acataacacc ttgtgtaaat gaggttatga gtacatgtga
     1621 acaacaaatc ttatgactaa actcgggtcg tgtacttttg acgattgagt catttttacc
     1681 aattaggcaa ttaatttttg ctaaattttc attaaccgcg ctggccatcc caacaaacaa
     1741 tataaaagct gacaacgaaa aaaaaaaaaa aaaaaaaaaa actccaattc tgaaaaattt
     1801 ttcttcttct tcatactaaa caaaacaact ataaagataa tcaattaata atgtcacaac
     1861 aacctagtga aaataaatta tggggtggtc gattcactgg cgccactgat ccattaatgg
     1921 atttatacaa tgcttcatta ccttatgata aagttatgta tgatgcagat ttaactggta
     1981 ctaaagtata tactcaagga ttgaacaaat taggattaat taccactgaa gaattgaatc
     2041 tgattcatca aggtttagaa caaattagac aagaatggca tgataataaa tttattatta
     2101 aagctggtga tgaagatatt catactgcta atgaacgtag attaggagaa atcattggga
     2161 aaaacatttc tggtaaagtt catactggta gatccagaaa tgatcaagtt gctactgata
     2221 tgagaatttt tgttcgtgaa tcattactta atttaagtaa gattttacat caattcatta
     2281 cagcaattct tgaacgagct cataaagaaa ttgatgtatt aatgcctggt tatactcatt
     2341 tacaaaaagc tcaacctatt agatgggctc attggttaag ttcatatgca acttatttca
     2401 ctgaagatta taaacgttta caagaaatca ttactcgtgt taatcaatct ccattaggat
     2461 ctggtgcttt agctggtcat ccctacggta ttgatcgtga atttttagct aaaggattag
     2521 gatttgatgg tgttattggg aattctttaa ctgcagtttc tgatcgtgat tttgttgttg
     2581 aaagtttatt ttggagtaca ttatttatga atcatatttc tcgattttca gaagatttaa
     2641 ttatttattc tagtggtgaa tttggattta taaagttagc tgatgcttac tctactggtt
     2701 catcattaat gccacaaaag aaaaatcctg attcattaga attattacgt gggaaaagtg
     2761 gcagagtgtt tggtcagtta tctgggtttt taatgtcaat taaatccatt ccatcaacct
     2821 ataataaaga tatgcaagaa gataaagaac cattatttga tgcattgact accgtagaac
     2881 attcaatttt aattgctact ggtgtgattt ctactttact gattgataaa caaaatatgg
     2941 aaaaagcttt aactatggat atgttagcta ctgatttagc agattattta gttagaaaag
     3001 gtgttccatt tagagaaact catcatattt ctggtgaatg tgttagaaaa gctgaagaag
     3061 aaaaattgtc gggaattgac caattatcat ttgaacaatt tcaacaaatt gattcacgtt
     3121 ttgaaaaaga tgttatggaa acttttgatt ttgaagctag tgtggaaaga agagatgcta
     3181 ttggtggtac tgctaagagc gctgttttaa aacagttaga aaatttgaaa tcaattttaa
     3241 attaatttat aaataatcat atagtattca atgttttgtt ttgttttttt tttttttttt
     3301 atggaaatgt acaaacagta tataatcaat taatagaaag ataccctgta ttccattgta
     3361 tcgcctttgg gatgtctata ataccaactt tcaagaatga gaaattaaac aacaatttct
     3421 ttcaccaatc aactcgtcta tattcaataa actcattctg gtcttttcaa atatttaatg
     3481 ttatcctgat caaacttaca aatcaaaaat gtatcaaaat cattcattag ttcttcttct
     3541 tttgttatct ttgtcactat atttctctcc atccagacca aaaatacttc cattgtgaag
     3601 atcgtctgaa tcataatcag caactacctc ttccatttca gagaattcag atttttcaaa
     3661 ataggtatca cgggccaagt acacacaaga caaacgatac gagtgctggg atatttcttt
     3721 caatgtatca ctaaattcgt tgagatttaa agagagccct ttgactgctt tcattacttt
     3781 cctcacatca tgagcatcca cattcaaatt tcctgtagcc ctttattctt agttttcttt
     3841 tcttattctt ttcttcgtac attgaaatca caaatggtga tatcaaacga aggtcacact
     3901 gacttatgtc taatacctcc aatgatgata ctggtaactt aaccaggtct actcttttca
     3961 aacgatttct cattagtgtc aatttacgta ggttcttcaa cctttcaaac gatgcaaata
     4021 gcttttgtaa gggcgaattc tgcagatatc catcacactg gtataacttc gtatagcata
     4081 cattatacga agttatggta atacggttat ccacagaatc gatccgtcta ggattgaacg
     4141 atgatattaa gcgagaattc acatttttct agaggaatca cagttacaat tgtagaggaa
     4201 accgaactag taaaactagc acccaacact tctaaacaaa ccaatcaatt caatggtata
     4261 acaaatcgtc taccagaagt tatgctaata tgaaacatca attgacaaca tggacatcaa
     4321 ctgaagcttt aacaaatgaa taaaaaaaaa agcccataca atagaccatt ggtcatagtt
     4381 tcatttgccc ccgccccact tttattgttg agttgaataa ttgtgagcat cagctgattt
     4441 taaaattata aatttttttt ttcgtccatt gttaattcta ttttagtttt ttcttctcca
     4501 tcgtctccga attattccgg aagctggtag cggataagtt ttagaatcat tgagcctctt
     4561 ttcaatgcca gatgttatct gcaactgatg gttagttagt tagtataaga cttaaattct
     4621 catataggtc atgtgcacgg acaagctcat tgagtgacga aaagtcaaat ttaaacttct
     4681 ctaaacaaat ttcagacaat agtccgaaaa aaaaaaacaa aacccattat tgtcgacatg
     4741 tcaaaggatt caacggttga gatggagccg tcaaatatcc aaaacactct tctttcaact
     4801 aatttgccgt aaactatctc catcgtacga ttaagataaa aaaaaacaat tatttcttac
     4861 aatatttgat agagacccaa tgcaaagcca gcgagaaaga ggagtattgg cattgttggg
     4921 tagtagatga atgagtaatc ttctagccaa aacaaaataa accacaagaa cagtaggcaa
     4981 aactgagtac ttgatagcgc ttgtagtctt tgtatttaaa aaaacctaat tgtccaaaaa
     5041 ttgaaaatca tgagttgcaa atttgttgaa atcaccaggt aaatagatca tttagctata
     5101 tcgccttatc attcttcgta caagaattat acacaaatac caccaccaca ccacccagaa
     5161 attttacacg tgttttctaa attattactt tccgtggcca atcaaattga aatttttctt
     5221 gttcgaaacg gcagtgcctc aaacattata gtagcaagta ttgcctaaag agtttccgtc
     5281 tcgtgccccc cccctatccc cttttgtaat ttgccaaaat agctcaaaaa ggataatccc
     5341 gcactttttt tttccgagtt ctcgtcatgc aagcaagctt attcagaatt ttcagaggtg
     5401 cgagtctcac cactaccact gcagctgcat cttttacagc aacagcaaca gcaggtgcca
     5461 ccacggcaaa gacattgtct ggatccactg tgctccgaaa actgtataaa aggacctatt
     5521 catccctggt cttatcttct ccagaattat ttttttttca tcagtttaac aacaacaaac
     5581 gttattgtca tacaacaaca acaacattat tcatgtccac acccgttccc gggttaatta
     5641 acggtgaaca aaagctaatc tccgaggaat acttgaacgg tgaacaaaaa ttaatctcag
     5701 aagaagactt gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg
     5761 gtgaacaaaa gctaatctcc gaggaagact tgaacggtga acaaaagtta atctcagaag
     5821 aagacttgaa cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg
     5881 aacaaaagct aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag
     5941 acttgaacgg actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac
     6001 aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact
     6061 tgaacggact cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa
     6121 agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatcaatcac gcatgcgatt
     6181 atagtctaag aactcgagga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7261 tccgcctcca tccagtctat taattgttgc cgggaagcta gag
//
