LOCUS       V008267                 6358 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008267
VERSION     V008267
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6358)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6358)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(435..1292)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1293..1397)
                     /label="AmpR promoter"
     CDS             2545..3438
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(3818..3851)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5437..5466
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     rep_origin      complement(join(6031..6358,1..261))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
       61 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
      121 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      181 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      241 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      301 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      361 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      421 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      481 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      541 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      601 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      661 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      721 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      781 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      841 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      901 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      961 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1021 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1081 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1141 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1201 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1261 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1321 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1381 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaataac
     1441 ttcgtatagc atacattata cgaagttata gctcggatcc actagtaacg gccgccagtg
     1501 tgctggaatt cgcccttgtt tgataccatg gcaagcatac tagaattatt atacgagctg
     1561 ggtgtgaaat gtgccaccgt ttatgcattt tcgatcgaaa acttcaagag actgcaatat
     1621 gaggtcaaat ggttgatgga attagccaaa tccaagttta cgcaaataaa ccaacatgga
     1681 ttgttgtgtg aggagtatgg ggttaaaatc agaatattag gaaataccaa acttttgccg
     1741 aaagatgtgt tagagatcct tgaaagaacc gaagaaataa ctaaaaacaa taagcgtgct
     1801 gtgttgaatg tctgcttccc atatacatca agagacgaga taacccatct gatcaagtca
     1861 gtagtagacg aagcgacgaa aggtggtatt gaaataaccg aggaggctat cgacaagcat
     1921 ctttatacga aagatgtgcc cccattggat ttactagtaa gaacatcagg aacatttaga
     1981 ctttcagact ttcttttatg gcaaacggtg tcgccagatt gtgcagttgt gtttgtcgac
     2041 aagttatggc cggcattcta gaccatggga tatgctcaag attttactca actgggggtt
     2101 taacaaatac atgtatggaa acccaaatgg atatggggtt agtaataact tggtagtgaa
     2161 gagccaaatg gaagaagaat taataaacac accaaatgga gctacagggc ttgaccggta
     2221 cctggaggac gaagagaccg aagttagtag taacaattta ggaactgaag aggatacagt
     2281 gacatccgaa gaagaagatg ttgaagatat aggcaaaaag taataggaca gtattttatt
     2341 tattcaatag tttttttttt ttttttgaaa cgtacacctt gtttatttca ggtgtttctt
     2401 tgaaacgtgt ctcaaagcga taacgggtac aaaaaaaaat gtattgagtc attgaaattt
     2461 ttttattttt tttttttttt ggtgaagatt tttcccacac caaatatata ccacttttct
     2521 tctaacacca cctacatatt cactatggat ttagtcaatc atttgcccga tcgtttgtta
     2581 tttgctgttc ccaaaaaggg cagattatat gaaaaatgct gcagcttatt gagcggtgcc
     2641 gatatacagt ttagaagatc taatagattg gatattgccc tttccacaaa cttgccaatt
     2701 gcattaatct ttttgcctgc agccgatatt ccagttttcg ttggagaagg taattgtgac
     2761 ttgggtataa ctgggttaga ccagatcaaa gaagcagacc aattcgacaa cattgaggat
     2821 ttgttggatt tgaagtttgg ctcatgtaaa ttgcagatcc aagttcctgc aaatggcgag
     2881 tacgaaaaac ccgaacaact cgttggaaag aagattgtat cctcctttac aaagttgagt
     2941 actgactatt tcaagaaatt atccgacaaa cctacgaata ttagatacgt tggtggttca
     3001 gttgaggctt cttgcgcatt gggtgttgcc gacgccattg tcgatttagt tgaaagtgga
     3061 gagaccatga aagctgctgg attaaaggcg atcgaaacca tattggaaac ttccgctcat
     3121 ttgatttcct ccaagaaaac caaatttcca gaaatggtca acataattgt ccaaagactt
     3181 caaggtgttt tagcggctca ggaatatgtc ttgtgtaact acaatgctcc aaaatccatt
     3241 caatcaaagt gcttaaccat tactccaggt agaagggcag ccactgtctc cactttggat
     3301 aaacacagtg acgacgaaga tgactgggtt gccatttctt ccatggttaa cagaaaggaa
     3361 attggtaatg taatggatga attgaagaaa gctggcgcaa cagatatatt ggtgctcgag
     3421 atatcaaatt gtagagttta ataaaatact atatatacat acttatagat ttacttcaat
     3481 ttgactcaat ctgtcttata ccatctttat ctaccacctt gatgtacaca cctttaaaat
     3541 caatgggcat tctcgtttgt aattctttga cacacatgtc cattaacttc aaaccatctt
     3601 caactgtcat gtcctttcta taatgtttat ctaacaagga tgttgtgtaa aatgctgcgt
     3661 agccatgagc accataagga cgttcaactt gcgtacctaa ataatctatc caattcaacg
     3721 acggctgatt tgtctttaca tcgtatcctc caattaaaca gttcacctgg tacggttttc
     3781 tagaagggcg aattctgcag atatccatca cactggtata acttcgtata gcatacatta
     3841 tacgaagtta tggtaatacg gttatccaca gaatcgatcc gtctaggatt gaacgatgat
     3901 attaagcgag aattcacatt tttctagagg aatcacagtt acaattgtag aggaaaccga
     3961 actagtaaaa ctagcaccca acacttctaa acaaaccaat caattcaatg gtataacaaa
     4021 tcgtctacca gaagttatgc taatatgaaa catcaattga caacatggac atcaactgaa
     4081 gctttaacaa atgaataaaa aaaaaagccc atacaataga ccattggtca tagtttcatt
     4141 tgcccccgcc ccacttttat tgttgagttg aataattgtg agcatcagct gattttaaaa
     4201 ttataaattt tttttttcgt ccattgttaa ttctatttta gttttttctt ctccatcgtc
     4261 tccgaattat tccggaagct ggtagcggat aagttttaga atcattgagc ctcttttcaa
     4321 tgccagatgt tatctgcaac tgatggttag ttagttagta taagacttaa attctcatat
     4381 aggtcatgtg cacggacaag ctcattgagt gacgaaaagt caaatttaaa cttctctaaa
     4441 caaatttcag acaatagtcc gaaaaaaaaa aacaaaaccc attattgtcg acatgtcaaa
     4501 ggattcaacg gttgagatgg agccgtcaaa tatccaaaac actcttcttt caactaattt
     4561 gccgtaaact atctccatcg tacgattaag ataaaaaaaa acaattattt cttacaatat
     4621 ttgatagaga cccaatgcaa agccagcgag aaagaggagt attggcattg ttgggtagta
     4681 gatgaatgag taatcttcta gccaaaacaa aataaaccac aagaacagta ggcaaaactg
     4741 agtacttgat agcgcttgta gtctttgtat ttaaaaaaac ctaattgtcc aaaaattgaa
     4801 aatcatgagt tgcaaatttg ttgaaatcac caggtaaata gatcatttag ctatatcgcc
     4861 ttatcattct tcgtacaaga attatacaca aataccacca ccacaccacc cagaaatttt
     4921 acacgtgttt tctaaattat tactttccgt ggccaatcaa attgaaattt ttcttgttcg
     4981 aaacggcagt gcctcaaaca ttatagtagc aagtattgcc taaagagttt ccgtctcgtg
     5041 ccccccccct atcccctttt gtaatttgcc aaaatagctc aaaaaggata atcccgcact
     5101 ttttttttcc gagttctcgt catgcaagca agcttattca gaattttcag aggtgcgagt
     5161 ctcaccacta ccactgcagc tgcatctttt acagcaacag caacagcagg tgccaccacg
     5221 gcaaagacat tgtctggatc cactgtgctc cgaaaactgt ataaaaggac ctattcatcc
     5281 ctggtcttat cttctccaga attatttttt tttcatcagt ttaacaacaa caaacgttat
     5341 tgtcatacaa caacaacaac attattcatg tccacacccg ttcccgggtt aattaacggt
     5401 gaacaaaagc taatctccga ggaatacttg aacggtgaac aaaaattaat ctcagaagaa
     5461 gacttgaacg gactcgacgg tgaacaaaag ttgatttctg aagaagattt gaacggtgaa
     5521 caaaagctaa tctccgagga agacttgaac ggtgaacaaa agttaatctc agaagaagac
     5581 ttgaacggac tcgacggtga acaaaagttg atttctgaag aagatttgaa cggtgaacaa
     5641 aagctaatct ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg
     5701 aacggactcg acggtgaaca aaagttgatt tctgaagaag atttgaacgg tgaacaaaag
     5761 ctaatctccg aggaagactt gaacggtgaa caaaaattaa tctcagaaga agacttgaac
     5821 ggactcgacg gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta
     5881 atctccgagg aagacttgaa cggtgaacaa aaattaatca atcacgcatg cgattatagt
     5941 ctaagaactc gaggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6001 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6061 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6121 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6181 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6241 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6301 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaaga
//
