LOCUS       V008270                 6294 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008270
VERSION     V008270
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6294)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6294)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(299..887)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1061..1918)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1919..2023)
                     /label="AmpR promoter"
     misc_feature    2125..2520
                     /note="5' region of ADE2 genes of Candida albicans; 5' of
                     ADE2"
     regulatory      2536..4008
                     /note="promoter region of ADH1 gene of Candida albicans;
                     CaADH1p"
                     /regulatory_class="promoter"
     regulatory      4022..4328
                     /note="terminator region of ACT1 gene of Candida albicans;
                     ACT1 terminator"
                     /regulatory_class="terminator"
     promoter        4366..4384
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4417..4433
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    4447..4480
                     /label="Cre recombinase binding site"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT); loxP"
     CDS             4910..5719
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    complement(5870..5903)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcggatctac cagttatggc agttggtgct cgtattttga aacaatttgg tgtgccattt
       61 gaactcacaa tcgtaagtgc acacagaact cctcatagaa tgtctgagta tgctattgaa
      121 gcaccaaagc gtggcttgaa atgtatcatt gctggtgcag gtggtgctgc tcatttgcca
      181 ggtatggtgt aatcatggtc ataccgcgga ggcctgggcc cgataacgca ggaaagaaca
      241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      721 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      961 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1021 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1081 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1141 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1201 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1261 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1321 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1381 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1441 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1501 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1561 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1621 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1681 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1741 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     1801 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     1861 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     1921 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1981 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2041 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     2101 tcacgaggcc aggtcgactc tagaaggtgg ccaattaggt cgtatgattg ttgaagcagc
     2161 acatagattg aatatcaaga ccgtaatttt ggacgcagcc aaatcaccag ctaaacagat
     2221 caatgctcta gacgatcacg ttgatggatc gttcacaaac tatgattcga ttgtgaaatt
     2281 agctgaaaag gcagacgtat taactgttga aatcgaacat gttgatgttg atgctttaat
     2341 caaagttcaa gagaaattcc ctaaagttga aatatatcca ttacctgaaa ccataagatt
     2401 gattcaagat aaatatttac agaaaaatca tttaatcaaa cacgatgttg cagttacaga
     2461 atctgttgca gttgaaacca acacagtcga tgacttgtta cacattgggg aaaagtttgg
     2521 cgatccgtct aggattgaac gatgatatta agcgagaatt cacatttttc tagaggaatc
     2581 acagttacaa ttgtagagga aaccgaacta gtaaaactag cacccaacac ttctaaacaa
     2641 accaatcaat tcaatggtat aacaaatcgt ctaccagaag ttatgctaat atgaaacatc
     2701 aattgacaac atggacatca actgaagctt taacaaatga ataaaaaaaa aagcccatac
     2761 aatagaccat tggtcatagt ttcatttgcc cccgccccac ttttattgtt gagttgaata
     2821 attgtgagca tcagctgatt ttaaaattat aaattttttt tttcgtccat tgttaattct
     2881 attttagttt tttcttctcc atcgtctccg aattattccg gaagctggta gcggataagt
     2941 tttagaatca ttgagcctct tttcaatgcc agatgttatc tgcaactgat ggttagttag
     3001 ttagtataag acttaaattc tcatataggt catgtgcacg gacaagctca ttgagtgacg
     3061 aaaagtcaaa tttaaacttc tctaaacaaa tttcagacaa tagtccgaaa aaaaaaaaca
     3121 aaacccatta ttgtcgacat gtcaaaggat tcaacggttg agatggagcc gtcaaatatc
     3181 caaaacactc ttctttcaac taatttgccg taaactatct ccatcgtacg attaagataa
     3241 aaaaaaacaa ttatttctta caatatttga tagagaccca atgcaaagcc agcgagaaag
     3301 aggagtattg gcattgttgg gtagtagatg aatgagtaat cttctagcca aaacaaaata
     3361 aaccacaaga acagtaggca aaactgagta cttgatagcg cttgtagtct ttgtatttaa
     3421 aaaaacctaa ttgtccaaaa attgaaaatc atgagttgca aatttgttga aatcaccagg
     3481 taaatagatc atttagctat atcgccttat cattcttcgt acaagaatta tacacaaata
     3541 ccaccaccac accacccaga aattttacac gtgttttcta aattattact ttccgtggcc
     3601 aatcaaattg aaatttttct tgttcgaaac ggcagtgcct caaacattat agtagcaagt
     3661 attgcctaaa gagtttccgt ctcgtgcccc ccccctatcc ccttttgtaa tttgccaaaa
     3721 tagctcaaaa aggataatcc cgcacttttt ttttccgagt tctcgtcatg caagcaagct
     3781 tattcagaat tttcagaggt gcgagtctca ccactaccac tgcagctgca tcttttacag
     3841 caacagcaac agcaggtgcc accacggcaa agacattgtc tggatccact gtgctccgaa
     3901 aactgtataa aaggacctat tcatccctgg tcttatcttc tccagaatta tttttttttc
     3961 atcagtttaa caacaacaaa cgttattgtc atacaacaac aacaacaacc gtgcatcgct
     4021 accattgttc accacaaatg tttctaagag tgaaattctg gaaatctgga aatctggttt
     4081 tgtattcttg ttattcttct ttttgttatt acatatataa cttgttactt ttttaaaaaa
     4141 atctttgttt attttataaa tatataaaac taaatttaag aaaaagagaa aaatgtttta
     4201 tttgagagat tgaaatttta cttgaattta gcttagcttt tataaagtat tattatgtaa
     4261 aaaaacaaaa caaatataca ttaaaaagtt aagactataa aatagccacc caaggcattt
     4321 ctatatctac agtctgccag acttaaaacg acggccagtg aattgtaata cgactcacta
     4381 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atccagatct
     4441 tgatccataa cttcgtatag catacattat acgaagttat agatccagta ctaataggaa
     4501 ttgatttgga tggtataaac ggaaacaaaa aaaagagctg gtactacttt ctttaaaatt
     4561 attttattat ttgattttat ttaatagtat atattatatt ttgaacgtag attattttgt
     4621 tgaaagttgc tgtagtgcca ttgattcgta acactaattc tgtattagtc attcctcttg
     4681 tttgatagta tccaaaaaaa cggctatttt tttgcaatct tatttcctgc atattataca
     4741 gataacataa tgaaagaaaa aatctttttt tttgttcttc aatgatgatt tcaaccattc
     4801 ttttaaacat tgatcaattc ctgagcaaca accccataca cactggttta tataccgccc
     4861 cttttacagt tgaagaaaga aatagaaata gaaatagcaa acaaaagata tgacagtcaa
     4921 cactaagacc tatagtgaga gagcagaaac tcatgcctca ccagtagcac agcgattatt
     4981 tcgattaatg gaactgaaga aaaccaattt atgtgcatca attgacgttg ataccactaa
     5041 ggaattcctt gaattaattg ataaattagg tccttatgta tgcttaatca agactcatat
     5101 tgatataatc aatgattttt cctatgaatc cactattgaa ccattattag aactttcacg
     5161 taaacatcaa tttatgattt ttgaagatag aaaatttgct gatattggta ataccgtaaa
     5221 gaaacaatat attggtggag tttataaaat tagtagttgg gcagatatta ccaatgctca
     5281 tggtgtcact gggaatggag tggttgaagg attaaaacag ggagctaaag aaaccaccac
     5341 caaccaagag ccaagagggt tattgatgtt agctgaatta tcatcagtgg gatcattagc
     5401 atatggagaa tattctcaaa aaactgttga aattgctaaa tccgataagg aatttgttat
     5461 tggatttatt gcccaacgtg atatgggtgg ccaagaagaa ggatttgatt ggcttattat
     5521 gacacctgga gttggattag atgataaagg tgatggatta ggacaacaat atagaactgt
     5581 tgatgaagtt gttagcactg gaactgatat tatcattgtt ggtagaggat tgtttggtaa
     5641 aggaagagat ccagatattg aaggtaaaag gtatagaaat gctggttgga atgcttattt
     5701 gaaaaagact ggccaattat aaatgtgaag ggggagattt tcactttatt agatttgtat
     5761 atatgtagaa taaataaata aataagttaa ataaataatt aaataagggt ggtaattatt
     5821 actatttaca atcaaaggtg gtccttctag aagatctgct agcccatgga taacttcgta
     5881 tagcatacat tatacgaagt tattagggat aacagggtaa tctgcaggaa ttcgcagtca
     5941 gtcagtcagg ctcatgttcg tgctgtcaca ggattaccta tgccaaaggg gttcactgaa
     6001 ttctccactt ccatcaccaa tgctattatg ttgaatgttt tgggtgataa ggcaactcca
     6061 aataaagaat tggagatttg tcgccgtgct ttggaaactc cacatgcgtc agtgtatctt
     6121 tatggtaaaa ctactagacc agagcgaaag atgggtcata tcaatgttgt cacatcttcc
     6181 atgcaagatg ccgagagtag attaagttat atattgggtg ataccactga aattcctaaa
     6241 tctttagcta ccgacaagga atctccattg gtgggaataa taatgggttc cgat
//
