LOCUS       62056_7905        3903 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pCOo-3, complete sequence.
ACCESSION   MN436814
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    44..50
                     /label=SapI
                     /note="SapI"
     misc_feature    52..54
                     /label=Gamma
                     /note="Gamma"
     misc_feature    56..59
                     /label=A
                     /note="A"
     misc_feature    61..66
                     /label=BsaI
                     /note="BsaI"
     protein_bind    131..152
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        167..197
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..221
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     229..245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(258..314)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(315..331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(666..671)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    673..676
                     /label=F
                     /note="F"
     misc_feature    678..680
                     /label=Epsilon
                     /note="Epsilon"
     misc_feature    complement(682..688)
                     /label=SapI
                     /note="SapI"
     misc_feature    692..731
                     /label=UNSX
                     /note="UNSX"
     CDS             complement(1100..1912)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1913..2060)
                     /label=BBa_P1003
                     /note="BBa_P1003"
                     /regulatory_class="promoter"
     rep_origin      2162..2750
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3180..3368)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc cgagctcttc atacaggagt
       61 gagaccattg acagcgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      121 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      181 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      241 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
      301 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
      481 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      541 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      601 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gacactagtg
      661 agcacggtct cacgctacag tgaagagcac cccaggatac atagattacc acaactccga
      721 gcccttccac ccgccggcga ggcccacgta gtgggccatc gccctgatag acggtttttc
      781 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      841 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      901 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      961 cgtttacaat ttctggcggc acgatggcat gagattatca aaaaggatct tcacctagat
     1021 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1081 tgacaggcta gtattattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     1141 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     1201 caccgaggca gttccaaaga atggcaaggt cctggtaacg gtctgcgatt ccgacccgtc
     1261 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     1321 caccatgagt gacgactgaa tccggtgaga atggcaagag cttgtgcatt tctttccaga
     1381 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     1441 tattcatgcg tgattgcgcc tgagcaagac gaaatacacg atcgctgtta aaaggacaat
     1501 tacaaacagg aatcgaatgt aaccggcgca ggaacacggc cagcgcatca acaatatttt
     1561 cacctgaatc aggatattct tctaatacct ggaaggctgt tttcccagga atcgcggtgg
     1621 tgagtaacca cgcatcatca ggagtacgga taaaatgctt gatggtcggg agaggcataa
     1681 actccgtcag ccagttgaga cggaccatct catctgtaac atcattggca acgctacctt
     1741 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg
     1801 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcgtccatgt
     1861 tggagtttaa gcgcggacgg gagcaagacg tttcccgttg aatatggctc ataacacccc
     1921 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     1981 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt
     2041 ttgctgagtt gaaggatcag ctatttagaa aaataaacaa ataggtcatg accaaaatcc
     2101 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2161 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2221 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2281 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2341 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2401 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2461 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2521 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2581 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2641 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2701 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2761 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2821 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2881 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggat gagcgcctga
     2941 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
     3001 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     3061 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3121 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     3181 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     3241 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     3301 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     3361 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
     3421 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
     3481 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
     3541 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     3601 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
     3661 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     3721 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     3781 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgctagt catgccccgc
     3841 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccctt
     3901 aag
//
