LOCUS       62056_7785        3907 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pCOe-3, complete sequence.
ACCESSION   MN436810
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    complement(44..49)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    51..54
                     /label=C
                     /note="C"
     misc_feature    56..58
                     /label=Alpha
                     /note="Alpha"
     misc_feature    complement(60..66)
                     /label=SapI
                     /note="SapI"
     protein_bind    130..151
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        166..196
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    204..220
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     228..244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(257..313)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(314..330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    665..671
                     /label=SapI
                     /note="SapI"
     misc_feature    673..675
                     /label=Omega
                     /note="Omega"
     misc_feature    677..680
                     /label=E
                     /note="E"
     misc_feature    682..687
                     /label=BsaI
                     /note="BsaI"
     misc_feature    691..730
                     /label=UNSX
                     /note="UNSX"
     CDS             complement(1096..1884)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      complement(1885..2032)
                     /label=specR promoter
                     /note="specR promoter"
                     /regulatory_class="promoter"
     rep_origin      2166..2754
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3184..3372)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc cgaggtctca aatgaatgtg
       61 aagagcttga cagcgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      121 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      181 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      241 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
      301 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      361 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      421 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
      481 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      541 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      601 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acactagtga
      661 gcacgctctt caggtagctt tgagaccacc ccaggataca tagattacca caactccgag
      721 cccttccacc cgccggcgag gcccacgtag tgggccatcg ccctgataga cggtttttcg
      781 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
      841 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
      901 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
      961 gtttacaatt tctggcggca cgatggcatg agattatcaa aaaggatctt cacctagatc
     1021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1081 gacaggctag tattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
     1141 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
     1201 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
     1261 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
     1321 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
     1381 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
     1441 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
     1501 gtcgatcgtg gctggctcga agataccagc aagaatgtca ttgcgctgcc attctccaaa
     1561 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
     1621 gacttctaca gcgcggagaa tctcactctc tccaggggaa gccgaagttt ccaaaaggtc
     1681 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
     1741 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
     1801 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
     1861 ttcggcgatc accgcttccc tcatgcgaaa cgatcctcat cctgtctctt gatcagatat
     1921 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     1981 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctaagcttgc
     2041 atgcctgcaa gtcgactcta gaggatattt agaaaaataa acaaataggt catgaccaaa
     2101 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2161 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2221 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2281 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2341 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2401 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2461 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2521 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2581 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2641 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2701 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2761 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2821 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2881 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggatgagcgc
     2941 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc
     3001 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     3061 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3121 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3181 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
     3241 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
     3301 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     3361 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc
     3421 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
     3481 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac
     3541 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca
     3601 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag
     3661 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
     3721 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
     3781 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc tagtcatgcc
     3841 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
     3901 ccttaag
//
