LOCUS       40924_12885       11986 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCOXS3-Bxb1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Thomson JG, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2011) Crop Improvement and Utilization Research 
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   2  (bases 1 to 11986)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11986)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-DEC-2011) Crop Improvement and Utilization Research Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(103..277)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(337..1125)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(1194..1871)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    2062..2083
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2098..2128
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2136..2152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2160..2176
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2805..2816
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
     CDS             3743..5245
                     /codon_start=1
                     /gene="Bxb1"
                     /product="Bxb1 recombinase"
                     /label=Bxb1
                     /note="Bxb1 large serine recombinase/integrase from 
                     Mycobacterium smegmati bacteriophage Bxb1"
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     gene            3743..5245
                     /gene="Bxb1"
                     /label=Bxb1
     primer_bind     3743..3765
                     /label=Bxb1 forward primer 'g' annealing site
                     /note="Bxb1 forward primer 'g' annealing site"
     primer_bind     complement(4444..4466)
                     /label=Bxb1 reverse primer 'h' annealing site
                     /note="Bxb1 reverse primer 'h' annealing site"
     regulatory      5299..5465
                     /label=CaMV 35S polyA terminator
                     /note="CaMV 35S polyA terminator"
                     /regulatory_class="polyA_signal_sequence"
     primer_bind     complement(5490..5506)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5709..5733
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7033..7659
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8096..9160
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9229..9423
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9767..9907
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10093..10681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10771..11562)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgactcta
      301 gctagaggat cgatccgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
      361 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
      421 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
      481 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
      541 gatattcggc aagcaggcat cgccatgtgt cacgacgaga tcctcgccgt cgggcatgcg
      601 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
      661 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
      721 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
      781 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
      841 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
      901 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgga gttcattcag
      961 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     1021 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     1081 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcccca tggtcgatcg
     1141 acagatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
     1201 gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg
     1261 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
     1321 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     1381 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
     1441 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
     1501 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     1561 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat
     1621 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     1681 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
     1741 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
     1801 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
     1861 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
     1921 cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct
     1981 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2041 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     2101 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     2161 aggaaacagc tatgaccatg attacgaatt cgagctcgaa cacgcgcttt attggctaaa
     2221 atctgcgcat gtgatatttt gttaaccatc ttagatcaac cataacgagg ataacgctct
     2281 tttgttcttt ttgcactttt ctctgataat ttcatgcaag attggaactt cgaaataatt
     2341 aatttttcaa aattaaaagt gcattttttt ttcatccgtt gtgcatatcc tcttagaaaa
     2401 atctcaaagg agacttttag tatgtagtaa tgtcaatatt gattttaaag aattatgaaa
     2461 aatatgtatg aataggaata ttattctcct aacttaagca cttcaatttg aaatctgtat
     2521 attcaagtat catagatttt tactttgaat tgaattcagt gtgtgtcaat gctaaaaaaa
     2581 tcgtctgaaa cttgataact tgatattatc ctccatattt caaagaaaaa agcagtcttt
     2641 ttatttgtcg ggaaaaagtt gtttaaagag ggacaagatt cttggtttaa ggatatagcg
     2701 taattattac tatgcataaa atgtcaatat aaaataactt aaggatatac gttgttatat
     2761 ttctatttaa attgattttg aggaaaagtc gttattatta gtgatgggaa ctacaatttt
     2821 cggtccaatt aacaaaatca gaattaaaaa aaggtgagcg gatggatagt ggtgacgagg
     2881 acatgatacg gatatgtttt aagagaatgg gtcttatcct cttttgccta gtaacagcac
     2941 catagggaat tgaaaaaaaa aactccctaa gaagcaaatc taaacaatag atactactat
     3001 tttaattaca caaaccgacc tttatcaata tttttatctt ctctgcaaaa cctttagacc
     3061 taatctcttt tttttatata tagtaaatcc ctattcactg gaacatctgt acatgacaag
     3121 aaaacatatg ttatcttatc aaactacaaa aatatactaa ttatagttag tttagaaatg
     3181 aaatttttgg tattatattg ttgattgtgt attattttgt aatgttatgg aaaacgaata
     3241 agactcaaaa taacaaagta aatggaaaaa gtgagttaag aaaaaccaaa aaaacaaaaa
     3301 ccaaaaagct gtgatatgat aatgacgggc catagaatga gaaccgcata tgggaaaaga
     3361 acacaataat agcaattaac atatgcgatg agataaacaa caataacaaa aaatttggag
     3421 ataaaacttg aaaagtgaaa tttatttatt ttaaaagggt caacgttatc cacggcttgc
     3481 acgtaagtaa tacccacaca tatatttatc ctttttttcc ttctctttac gcttttttaa
     3541 aatttaaaat aaagaagaaa aaaaactcaa aaaactcttc acatatccca tatatatgtg
     3601 tagatatcta ttctgcaaat agaactcacg tttctttgtt tccaattgta gaataacaca
     3661 caactttatt tctcctgttc aagactcctt tattttctct tatgttcaaa gagatgaatc
     3721 tgaatctgaa gcggcgcgcc ccatgagagc cctggtagtc atccgcctgt cccgcgtcac
     3781 cgatgctacg acttcaccgg agcgtcagct ggagtcttgc cagcagctct gcgcccagcg
     3841 cggctgggac gtcgtcgggg tagcggagga tctggacgtc tccggggcgg tcgatccgtt
     3901 cgaccggaag cgcagaccga acctggcccg gtggctagcg ttcgaggagc aaccgttcga
     3961 cgtgatcgtg gcgtaccggg tagaccggtt gacccgatcg atccggcatc tgcagcagct
     4021 ggtccactgg gccgaggacc acaagaagct ggtcgtctcc gcgaccgaag cgcacttcga
     4081 tacgacgacg ccgtttgcgg cggtcgtcat cgcgcttatg ggaacggtgg cgcagatgga
     4141 attagaagcg atcaaagagc ggaaccgttc ggctgcgcat ttcaatatcc gcgccgggaa
     4201 ataccgagga tccctgccgc cgtggggata cctgcctacg cgcgtggacg gggagtggcg
     4261 gctggtgccg gaccctgtgc agcgagagcg catcctcgag gtgtatcacc gcgtcgtcga
     4321 caaccacgag ccgctgcacc tggtggccca cgacctgaac cggcgtggtg tcctgtcgcc
     4381 gaaggactac ttcgcgcagc tgcaaggccg cgagccgcag ggccgggagt ggtcggctac
     4441 cgcgctgaag cgatcgatga tctccgaggc gatgctcggg tacgcgactc tgaacggtaa
     4501 gaccgtccga gacgacgacg gagccccgct ggtgcgggct gagccgatcc tgacccgtga
     4561 gcagctggag gcgctgcgcg ccgagctcgt gaagacctcc cgggcgaagc ccgcggtgtc
     4621 taccccgtcg ctgctgctgc gggtgttgtt ctgcgcggtg tgcggggagc ccgcgtacaa
     4681 gttcgccggg ggaggacgta agcacccgcg ctaccgctgc cgctcgatgg ggttcccgaa
     4741 gcactgcggg aacggcacgg tggcgatggc cgagtgggac gcgttctgcg aggagcaggt
     4801 gctggatctg ctcggggacg cggagcgtct ggagaaagtc tgggtagccg gctcggactc
     4861 cgcggtcgaa ctcgcggagg tgaacgcgga gctggtggac ctgacgtcgc tgatcggctc
     4921 cccggcctac cgggccggct ctccgcagcg agaagcactg gatgcccgta ttgcggcgct
     4981 ggccgcgcgg caagaggagc tggagggtct agaggctcgc ccgtctggct gggagtggcg
     5041 cgagaccggg cagcggttcg gggactggtg gcgggagcag gacaccgcgg caaagaacac
     5101 ctggcttcgg tcgatgaacg ttcggctgac gttcgacgtc cgcggcgggc tgactcgcac
     5161 gatcgacttc ggggatctgc aggagtacga gcagcatctc aggctcggca gcgtggtcga
     5221 acggctacac accgggatgt cgtagactag ttctagagtc cgcaaaaatc accagtctct
     5281 ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata
     5341 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
     5401 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     5461 ccagtgacct gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     5521 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5581 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5641 gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     5701 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     5761 aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     5821 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
     5881 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     5941 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     6001 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     6061 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     6121 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     6181 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     6241 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     6301 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     6361 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     6421 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     6481 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     6541 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     6601 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     6661 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     6721 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     6781 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     6841 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     6901 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     6961 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     7021 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     7081 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     7141 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     7201 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     7261 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     7321 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     7381 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     7441 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     7501 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     7561 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     7621 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     7681 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     7741 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     7801 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     7861 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     7921 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     7981 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     8041 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg
     8101 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     8161 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     8221 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     8281 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     8341 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     8401 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     8461 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     8521 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     8581 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     8641 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     8701 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     8761 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     8821 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     8881 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     8941 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     9001 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     9061 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     9121 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     9181 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     9241 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     9301 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     9361 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     9421 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     9481 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     9541 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     9601 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     9661 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     9721 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     9781 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     9841 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     9901 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10501 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    10561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10741 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
    10801 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
    10861 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    10921 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    10981 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    11041 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    11101 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    11161 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    11221 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    11281 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    11341 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    11401 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    11461 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    11521 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    11581 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    11641 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    11701 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    11761 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    11821 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    11881 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    11941 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctgg
//
