LOCUS       V008298                14903 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008298
VERSION     V008298
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14903)
  AUTHORS   Staddon JH, Bryan EM, Manias DA, Dunny GM.
  TITLE     Conserved target for group II intron insertion in relaxase genes of
            conjugative elements of gram-positive bacteria
  JOURNAL   J. Bacteriol. 186 (8), 2393-2401 (2004)
   PUBMED   15060042
REFERENCE   2  (bases 1 to 14903)
  AUTHORS   Manias DA, Staddon JH, Klein JR, Dunny GM.
  TITLE     A new mobilizable shuttle plasmid based on pCOM9
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 14903)
  AUTHORS   Manias DA, Staddon JH, Klein JR, Dunny GM.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2003) Microbiology, University of Minnesota, MMC
            196, 420 Delaware Street S.E., Minneapolis, MN 55455, USA
REFERENCE   4  (bases 1 to 14903)
  AUTHORS   Manias D, Dunny G.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2007) Microbiology, Univ. Minnesota, 420 Delaware
            St., Minneapolis, MN 55455, USA
REFERENCE   5  (bases 1 to 14903)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 14903)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2004"; volume: "186"; issue: "8"; pages:
            "2393-2401"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (20-MAY-2003) Microbiology, University of Minnesota, MMC 196, 420
            Delaware Street S.E., Minneapolis, MN 55455, USA"
            SGRef: number: 4; type: "Journal Article"; journalName: "Submitted
            (05-MAR-2007) Microbiology, Univ. Minnesota, 420 Delaware St.,
            Minneapolis, MN 55455, USA"
            SGRef: number: 5; type: "Journal Article"
            On Mar 5, 2007 this sequence version replaced AY303238.1.
FEATURES             Location/Qualifiers
     source          1..14903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     oriT            1069..1510
                     /label="pRS01 origin of transfer"
                     /note="pRS01 origin of transfer"
     CDS             2123..2890
                     /codon_start=1
                     /gene="AAD(9)"
                     /product="spectinomycin adenyltransferase"
                     /label="AAD(9)"
                     /note="spectinomycin resistance"
                     /protein_id="AAP60024.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            2123..2890
                     /gene="AAD(9)"
                     /label="AAD(9)"
     terminator      5596..5623
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      5755..5798
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(6094..6121)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(6213..6299)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     6773..6789
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    6808..6912
                     /label="ltrE, 3' fragment"
                     /note="ltrE, 3' fragment"
     CDS             6955..7311
                     /codon_start=1
                     /product="LtrF"
                     /function="unknown"
                     /label="LtrF"
                     /protein_id="AAP60025.1"
                     /translation="MKNKQNLKRPIQKIIRFSKEENDYLKDKIERSPFNNFQNYTRILL
                     LTGEVKIVDYTGLQKLNGEVNRIGNNINQIAKLAHQFKEISGEDINDLSQQLSEIQVMI
                     SERFKEELKQERLI"
     CDS             8364..10160
                     /gene="ltrA"
                     /label="Group II intron-encoded protein LtrA"
                     /note="Group II intron-encoded protein LtrA from
                     Lactococcus lactis subsp. cremoris (strain MG1363).
                     Accession#: P0A3U1"
     CDS             10410..11201
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             12891..14582
                     /codon_start=1
                     /product="LtrG*"
                     /label="LtrG*"
                     /protein_id="AAP60028.1"
                     /translation="MKLSFLHNYEVTTLAKAIYSITSWFPNRQFLGIVDKLNRDFIDNE
                     DKILGTIKIDSYTEFLNFYNQVLESYLPENPPEPFPIDKGNVKFYSNEEFHKIFIGNSS
                     EDIYEVSFIIESLIHDHESLKEIWYEILRYENLVISNLEQFKNSFIQEGNFECPPKDYF
                     YFIFNKFDEFRNDKLDEYFKEFKSVNVELYKFFSPVNKLPIFLPMMKECFIERVEEEIK
                     EEEFKESIWRSFYRILNCNFPLFQRAGGNIFYYLKLIHKDTKEEIDLENTIALLNENNL
                     IILESNISNIPEHIKVGIKNSLYRIAGLCQDGRVRVFEFRTECNIVFMGVDSEGISPNI
                     NKSIFLSRNEKIIFNASSIVGIINSAVDIKSIIDFLINYRKADDKMISFANIDAHFRTW
                     QLSNQVINEGASDLTIFYSTYESVHSNMDFFDRLQNLYPFELEGNFKNIHSWEIVEKED
                     TDLSLNSKVNSGSVDLFVVANKKIIYQEFDFILNDLDISDYEKISSFNEIILNALNKNK
                     KNILSNISGSILEINLVSQDILQKNSNHRYPIMNANYLESTCRSLA"
     misc_feature    12891..14551
                     /note="identical to pRS01 deposited under GenBank Accession
                     Number U50902"
     misc_feature    14552..14582
                     /label="from pDL281 vector"
                     /note="from pDL281 vector"
     primer_bind     complement(14588..14604)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    14612..14628
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(14636..14666)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(14681..14702)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcct agaatgacat
     1081 ctaggacaaa cttccgtagt cgtttccaca tctggtttct tttttttaac attgtaaaca
     1141 agctcattgc gcccctcctt caacctaata caattataac agaaaaagca aacatccctg
     1201 acaggcagga ggtcattcca ttacccccta cacccccttt gggaggaaga gcctatccct
     1261 tccttgacct tttgcaagca tagtaagatg atagcatctt acggaattta agaaatggat
     1321 aaggtttctt ccttatccat ttacttaatt tttgggggtg tccccaatcc ccatttttta
     1381 tgaaaagtag tttctacttc gcattttttc tttttccttg taaccaagaa aaatcagcca
     1441 acatactttt ggctttttgt tctttgccct ctcgtttttc gaggtcatct gaagaagttg
     1501 tttgaatctc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1561 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1621 ccagccggaa gggccgagcg cagaagtggt ccgataaacc cagcgaacca tttgaggtga
     1681 taggtaagat tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg
     1741 aagcgccata tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt
     1801 gccttgaata tattgacaat actgataaga taatatatct tttatataga agatcgattt
     1861 tcgttcgtga atacatgtta taataactat aactaataac gtaacgtgac tggcaagaga
     1921 tatttttaaa acaatgaata ggtttacact tactttagtt ttatggaaat gaaagatcat
     1981 atcatatata atctagaata aaattaacta aaataattat tatctagata aaaaatttag
     2041 aagccaatga aatctataaa taaactaaat taagtttatt taattaacaa ctatggatat
     2101 aaaataggta ctaatcaaaa tagtgaggag gatatatttg aatacatacg aacaaattaa
     2161 taaagtgaaa aaaatacttc ggaaacattt aaaaaataac cttattggta cttacatgtt
     2221 tggatcagga gttgagagtg gactaaaacc aaatagtgat cttgactttt tagtcgtcgt
     2281 atctgaacca ttgacagatc aaagtaaaga aatacttata caaaaaatta gacctatttc
     2341 aaaaaaaata ggagataaaa gcaacttacg atatattgaa ttaacaatta ttattcagca
     2401 agaaatggta ccgtggaatc atcctcccaa acaagaattt atttatggag aatggttaca
     2461 agagctttat gaacaaggat acattcctca gaaggaatta aattcagatt taaccataat
     2521 gctttaccaa gcaaaacgaa aaaataaaag aatatacgga aattatgact tagaggaatt
     2581 actacctgat attccatttt ctgatgtgag aagagccatt atggattcgt cagaggaatt
     2641 aatagataat tatcaggatg atgaaaccaa ctctatatta actttatgcc gtatgatttt
     2701 aactatggac acgggtaaaa tcataccaaa agatattgcg ggaaatgcag tggctgaatc
     2761 ttctccatta gaacataggg agagaatttt gttagcagtt cgtagttatc ttggagagaa
     2821 tattgaatgg actaatgaaa atgtaaattt aactataaac tatttaaata acagattaaa
     2881 aaaattataa aaaaattgaa aaaatggtgg aaacactttt ttcaattttt ttgttttatt
     2941 atttaatatt tgggaaatat tcattctaat tggtaatcag attttagaaa acaataaacc
     3001 cttgcataac tttctcgtcc atatcggaaa ctactttttt attccctttt tttcgaccga
     3061 gattattttg cgataaaatc cgattaagat actgcctact aactcccaat tcttgagcta
     3121 attctgatac tgttttactc atcagctacc tcacaaaata attctttcta tcccaattcc
     3181 aaaaggcaac aatttcacga ccagtacttt ctgcagattc ttctgctccg gtctgaacaa
     3241 gattcccttc ttcgacatca tcaagagcta actccgcctt gattttcttg aacaactttc
     3301 caaaaccaat ctgccttttt cggtgcaaac cattcgttaa atcttctgtt atccgttctt
     3361 tgaccatttc agagaataat gtcacatctt ctgtatctac atcaaaaggc ttaacaggat
     3421 atttagctgt ttcgataata gctgacttca agtttttacg tttatgagct ttgacggttc
     3481 gaatatcaac tacaggtgta taatccaatt tcatcgcctt agcccatagt ttcgtccatt
     3541 cttcttggct aatgtaattt gtattatttc cagtaaaata gctagatttc acaaataaca
     3601 atacatgcaa atgagggtga taattctcat gctcagttga ataagttacc tcagtggctc
     3661 gcaaatagcc aataacattt ttatcgactt tcttgtattt cataagacga ttaaaagctc
     3721 gccctatttc tgaaattgat ttatttaatt tttcaccact aatgttctta atcgtcaagg
     3781 tcaaaaagag aaagcgacct tttggatagc gtttcattgc ttcatttacc actaattcag
     3841 cttgataagc atacttcatt gaccgtctcc agttacaaat tgggcagagc ttatttttgc
     3901 aaaaataaga ctgatagagt tttttcgtgc cgtcttgttg ttcgataaac ttcaacactt
     3961 cggcacattg atagacccgt tcaaacgaac gatatcctaa acgctctaaa cgaccagcaa
     4021 gctcaatatt ttttaacttg cgttctcgcc actttctatc tttgccattc ttagaatagt
     4081 ccttaaagac ttgattttca gtacctgtca tgttataatt ttcttgcaaa aaaatttttt
     4141 tgagtatata aaaaagttcc tgcaattctc ctaagtttat tattttaaat tggacactta
     4201 aattataaac tttattttat taaattgcaa gggctttttt gttacctaaa aacccagcaa
     4261 tatcaagggt ttgaggtgct ttaaaacaag aaaataaaaa aactcattat ttctatatgt
     4321 atcaagataa gaaaaaaccc ttgctacgca attgggtttt taagggcttt tcagccctct
     4381 ttttcgtttc acgaaaaatt cacccctaaa acctcaaatt ttgactttct aaatccaaac
     4441 gtggtataat aattttaacg ctagaagttc taggaaacga atagcacaac gcaaaaaaaa
     4501 cagtagctgg aactactgtc tttctttttt gtcctcaaaa agaactcagg ttaatttttg
     4561 tcgcgtttgt ttcgaacata ccgaatcaac caatcagcac ttacctctgc agcaacgcca
     4621 accacaaaag gtatcaaaac aagttctagg aaagtcttca cagcgctcac ctccaatcta
     4681 agttcgtaac ttagcgaaag aacgttgcga cctagctatt ataccatgtc cgcttacttt
     4741 attccatggt ctaattgaca acaagtaacc aagggcggac gtcctttgtc cgtgtcggct
     4801 cgaaacgcta aagcctttcg gctcgtcacg tcctagcgta ctttgccctt gcttattgtc
     4861 aattagcttt cacggcataa atcgctcaaa ggcctagccc tttttcaatc actcgtttaa
     4921 ctacccttac gattggctga atagctctta actctgatat aaaatttttt aaggctttat
     4981 ctggcataaa ctctttcagt tttcctagca ttttgtcatt ttgctgtctc aaaactgagt
     5041 tttcacttct aagcgctcta ttttcgttcc taagacgctc tatgctctct cccttacttt
     5101 tttctttagc tcgttccaac tcgcttcaaa ccgtttagag cgtctcagag gcactatccg
     5161 ccttaattgc tcattctcta gctctttttg atgaacaagg tttctgcttc ggtatatcag
     5221 ccctttaaat tcttcaaact gctctttagt caattccaca tattctttac cgaacacgcc
     5281 ttttttaggg gtaatagccg ctagttcttt ctcagacatg atttttaggt cagctatttg
     5341 tgtttttagt ttttctaatt tcagcccctc gcgctctaaa cgctctattt tcgcttctaa
     5401 gctcttcgaa taattttcta aggtatttat atatccctca gctttagata gctctgagag
     5461 cctctcagag gtctcagaat cgccaggacc caacgctgcc cgactgcttt cctgatgcaa
     5521 aaacgaggct agtttaccgt atctgtgggg ggatggcttg tagatatgac gacaggaaga
     5581 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg
     5641 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa
     5701 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     5761 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac
     5821 tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg
     5881 catggggtca ggtgggacca ccgcgctact gccgccaggc aaattctgtt ttatcagacc
     5941 gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca
     6001 gctgctttcc tgatgcaaaa acgaggctag tttaccgtat ctgtgggggg atggcttgta
     6061 gatatgacga caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
     6121 tgcttaattt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
     6181 tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
     6241 gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga
     6301 tgcctggcag ttccctactc tcgcatgggg agaccccaca ctaccatcgg cgctacggcg
     6361 tttcacttct gagttcggca tggggtcagg tgggaccacc gcgctactgc cgccaggcaa
     6421 attctgtttt atcagaccgc ttctgcgttc tgatttaatc tgtatcaggc tgaaaatctt
     6481 ctctcatccg ccaaaacagc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg
     6541 atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg
     6601 gctccaattc ttggagtggt gaataattcc cggcattcgc cattcaggct gcgcaactgt
     6661 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6721 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6781 acggccagtg aattcgagct cggtacccta gagcgaataa aactttataa atccataaat
     6841 ataaatatta gaatttgcgg ggatttatca ccaacaacac aattcttaaa aagaattgaa
     6901 tatataaaat aggaagatta tcataatgat agtctttttt tgaggattta aaatttgaaa
     6961 aataaacaaa atttaaaaag accaattcaa aaaattatta gattttctaa agaggagaat
     7021 gactacctga aagataaaat agagcgaagt ccattcaata attttcaaaa ctatactcgt
     7081 attttgttgc tcacaggcga agttaaaatt gttgattata ctggtcttca gaaacttaat
     7141 ggagaagtca atcgaatcgg aaataatatt aatcagattg ctaaacttgc tcatcaattt
     7201 aaggaaatat caggagaaga tattaatgat ttatctcagc agctctctga aattcaagta
     7261 atgatttcag aacgatttaa agaagaattg aaacaagaaa gattaatcta atggtttaca
     7321 caaaacacat tattgttcat aaattaaaac atttacgcca ggcaaaagac tatgtagaaa
     7381 atgcggaaaa aactttggtg aatgaaagta acgaagatca tctcacaaat ctttttcctt
     7441 atatttcaaa tccagacaag acaatgagta agcaacttgt ttctggtcat ggtatcacaa
     7501 atgtttatga tgcggccaat gaatttattg cgacaaaaaa attaaaagca ctatccaaag
     7561 gaactgactt taattttgat ccgcaaactg gaaaggtaag attcaatgta gaaagcttag
     7621 agaaaaataa tgcggtgctt ggtcatcacc tcatccaatc attttctcct gatgacaatc
     7681 taactcctga acaaattcat gaaataggtc gtcaaaccat attagaattt acaggtggcg
     7741 aatatgaatt tgtgattgca acccacgtcg atcgtgaaca catccataac gtgcgcccag
     7801 atagggtgtt aagtcaagta gtttaaggta ctactctgta agataacaca gaaaacagcc
     7861 aacctaaccg aaaagcgaaa gctgatacgg gaacagagca cggttggaaa gcgatgagtt
     7921 acctaaagac aatcgggtac gactgagtcg caatgttaat cagatataag gtataagttg
     7981 tgtttactga acgcaagttt ctaatttcgg ttatgtgtcg atagaggaaa gtgtctgaaa
     8041 cctctagtac aaagaaaggt aagttatggt tgtggactta tctgttatca ccacatttgt
     8101 acaatctgta ggagaaccta tgggaacgaa acgaaagcga tgccgagaat ctgaatttac
     8161 caagacttaa cactaactgg ggatacccta aacaagaatg cctaatagaa aggaggaaaa
     8221 aggctatagc actagagctt gaaaatcttg caagggtacg gagtactcgt agtagtctga
     8281 gaagggtaac gccctttaca tggcaaaggg gtacagttat tgtgtactaa aattaaaaat
     8341 tgattaggga ggaaaacctc aaaatgaaac caacaatggc aattttagaa agaatcagta
     8401 aaaattcaca agaaaatata gacgaagttt ttacaagact ttatcgttat cttttacgtc
     8461 cagatattta ttacgtggcg tatcaaaatt tatattccaa taaaggagct tccacaaaag
     8521 gaatattaga tgatacagcg gatggcttta gtgaagaaaa aataaaaaag attattcaat
     8581 ctttaaaaga cggaacttac tatcctcaac ctgtacgaag aatgtatatt gcaaaaaaga
     8641 attctaaaaa gatgagacct ttaggaattc caactttcac agataaattg atccaagaag
     8701 ctgtgagaat aattcttgaa tctatctatg aaccggtatt cgaagatgtg tctcacggtt
     8761 ttagacctca acgaagctgt cacacagctt tgaaaacaat caaaagagag tttggcggcg
     8821 caagatggtt tgtggaggga gatataaaag gctgcttcga taatatagac cacgttacac
     8881 tcattggact catcaatctt aaaatcaaag atatgaaaat gagccaattg atttataaat
     8941 ttctaaaagc aggttatctg gaaaactggc agtatcacaa aacttacagc ggaacacctc
     9001 aaggtggaat tctatctcct cttttggcca acatctatct tcatgaattg gataagtttg
     9061 ttttacaact caaaatgaag tttgaccgag aaagtccaga aagaataaca cctgaatatc
     9121 gggaacttca caatgagata aaaagaattt ctcaccgtct caagaagttg gagggtgaag
     9181 aaaaagctaa agttctttta gaatatcaag aaaaacgtaa aagattaccc acactcccct
     9241 gtacctcaca gacaaataaa gtattgaaat acgtccggta tgcggacgac ttcattatct
     9301 ctgttaaagg aagcaaagag gactgtcaat ggataaaaga acaattaaaa ctttttattc
     9361 ataacaagct aaaaatggaa ttgagtgaag aaaaaacact catcacacat agcagtcaac
     9421 ccgctcgttt tctgggatat gatatacgag taaggagaag tggaacgata aaacgatctg
     9481 gtaaagtcaa aaagagaaca ctcaatggga gtgtagaact ccttattcct cttcaagaca
     9541 aaattcgtca atttattttt gacaagaaaa tagctatcca aaagaaagat agctcatggt
     9601 ttccagttca caggaaatat cttattcgtt caacagactt agaaatcatc acaatttata
     9661 attctgaatt aagagggatt tgtaattact acggtctagc aagtaatttt aaccagctca
     9721 attattttgc ttatcttatg gaatacagct gtctaaaaac gatagcctcc aaacataagg
     9781 gaacactttc aaaaaccatt tccatgttta aagatggaag tggttcgtgg ggcatcccgt
     9841 atgagataaa gcaaggtaag cagcgccgtt attttgcaaa ttttagtgaa tgtaaatccc
     9901 cttatcaatt tacggatgag ataagtcaag ctcctgtatt gtatggctat gcccggaata
     9961 ctcttgaaaa caggttaaaa gctaaatgtt gtgaattatg tggaacatct gatgaaaata
    10021 cttcctatga aattcaccat gtcaataagg tcaaaaatct taaaggcaaa gaaaaatggg
    10081 aaatggcaat gatagcgaaa caacgtaaaa ctcttgttgt atgctttcat tgtcatcgtc
    10141 acgtgattca taaacacaag tgaatgtcga ctaatgcttg aaacccagga caataacctt
    10201 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
    10261 tacgccaact ttcaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
    10321 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
    10381 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
    10441 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
    10501 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
    10561 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
    10621 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
    10681 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
    10741 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
    10801 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
    10861 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
    10921 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
    10981 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
    11041 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
    11101 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
    11161 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
    11221 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
    11281 actgtcgaca gtgaattttt acgaacgaac aataacagag ccgtatactc cgagaggggt
    11341 acgtacggtt cccgaagagg gtggtgcaaa ccagtcacag taatgtgaac aaggcggtac
    11401 ctccctactt caccatatca tttttaattc tacgaatctt tatactggca aacaatttga
    11461 ctggaaagtc attcctaaag agaaaacaaa aagcggcaaa gcttatgatg tgaccaaaaa
    11521 taattttgaa aaagtctctg ataaaattgc ttcacgttat ggcgcaaaaa ttattgaaaa
    11581 gtctccaggt aactctcact taaaatatac aaaatggcaa actcagtcca tttataaaag
    11641 tcaaatcaaa caacggttag actatctttt agaaatgtca tcagatattg aagacttcaa
    11701 aagaaaagca caggctttaa atttatcctt tgatttttct ggcaagtgga ccacttatcg
    11761 gctgcttgat gaacctcaaa tgaaaaacac acgtggacgg aatcttgata aaaatcgtcc
    11821 tgaaaaatac aatttggaat ctattattga acggctcgaa acaaatgagc tttcacttac
    11881 ggttgatgaa gtagtggagc ggtatgagga aaaagttgac gtagttaaac aagattttga
    11941 ttatcaagtt acggttgaaa aagggcaaat tgaccacatg acaagtaaag gattttacct
    12001 taacgttgac tttgggattg cggatcgtgg tcaaattttt attggcggtt ataaagttga
    12061 ccaacttgaa aatagggact gcgtgcttta tctgaagaag aatgaaacct ttcgtctttt
    12121 gagtgaaaag gaggcctcct tcaccaagta tttgactggt catgacctcg caaaacagtt
    12181 agggctttat aatggaactg ttcctttaaa gaaagaacct gtgatatcca caattaatca
    12241 gttggtcgat gcgattaact ttttagctga acacggcgtt actgaaggga ctcaatttaa
    12301 taatatggag agccaactca tgagcgctct tggggaggcg gaagaaaaac tttatgttat
    12361 tgataataaa attatggagt taaccaaaat tgcaaagtta ctcattgaaa aagagtcgga
    12421 tcattctcaa gcggttatta atgaattaga gaatctagga gtgggtccgt caataaaata
    12481 tcaagacatt caccaagaac ttcagtctga aaaaatgagt cgaaaaatac tcaaaaacaa
    12541 atttgaacaa accgttgatg agattaatac tttcaatgaa attagagtta ctacccttga
    12601 agaaaataaa ggaaaaatac tataatttct tacgctatca aaactataac agaaatttgt
    12661 atagtttatg aacggatgta gtaagtttca taataaataa ttagaactgc tatgaagtta
    12721 agttatgagt agtttttatt ccacgaaggt ttgctatttt attttaaatg aatagacaga
    12781 ggttgtaaaa aaagttgaat ccttgtagaa ttaagataaa ataaattaaa gatgttgttc
    12841 agcgttagaa ttaaacagtt gatttaaata tctttgacta ggagattgat atgaaacttt
    12901 catttttaca caactatgag gtaactactt tagcaaaggc tatttattct attacatcat
    12961 ggtttccaaa tagacaattt ttaggaatag ttgataaatt aaatagggat tttatagata
    13021 atgaagataa aattttaggt acaataaaaa ttgactcata tactgagttt ttaaattttt
    13081 ataatcaagt acttgagagt tatcttccgg agaatccacc tgaaccattt ccaattgata
    13141 aggggaatgt aaagttttat tctaatgaag agtttcataa aatttttata ggaaatagtt
    13201 cagaagatat ttatgaggtt tcgttcatta tagaatcctt aattcatgac catgaaagtt
    13261 taaaagaaat atggtatgaa attctaaggt atgaaaacct agttatttca aacttggaac
    13321 agttcaagaa tagttttatt caagaaggaa attttgaatg tccccctaaa gattattttt
    13381 attttatatt taataagttt gatgaattca gaaacgataa attggatgaa tactttaagg
    13441 aattcaaaag tgtaaacgtt gaactttata aattttttag tcctgtaaat aaactcccta
    13501 tatttttacc tatgatgaaa gaatgtttta ttgaaagagt agaggaggaa ataaaagaag
    13561 aagagtttaa agagtcgatt tggcgtagtt tttatagaat cttaaattgc aattttccgc
    13621 tttttcaaag agctgggggc aatatattct attatctaaa actgattcat aaagatacta
    13681 aggaagaaat tgatttagaa aatactattg cacttttgaa tgaaaataat ttaatcatac
    13741 tagaatcaaa tataagcaat atacctgaac atataaaagt tgggataaaa aatagcctat
    13801 atcgaatagc tggcctatgt caagatggca gagtcagagt ttttgaattt agaacagaat
    13861 gcaatatagt attcatggga gttgattcag aaggtatatc ccctaatatt aacaaatcaa
    13921 tttttttaag tagaaacgag aaaataatat ttaatgcaag tagtatagtt ggaataataa
    13981 atagtgcagt agatattaag agtatcatag attttttaat aaattataga aaagctgatg
    14041 ataaaatgat atcatttgcc aacatagatg cacactttag aacatggcaa ttaagcaatc
    14101 aagtaattaa tgagggagct tcggatttaa caatatttta ttccacgtat gaaagtgttc
    14161 attcaaatat ggatttcttt gataggcttc agaatttata cccttttgag ttagagggaa
    14221 attttaaaaa tattcacagt tgggaaatag ttgagaaaga agatactgat ctatctttaa
    14281 attcaaaagt aaactctggt tcagttgatt tatttgtagt agctaataaa aaaattattt
    14341 atcaagagtt cgattttata ttgaatgatt tagatataag tgattatgag aaaataagtt
    14401 cattcaatga aatcattcta aatgctttga ataaaaataa aaaaaatatt ttatcaaata
    14461 tttctggtag tattttagaa atcaatttag tttcgcaaga tatacttcaa aaaaattcga
    14521 accacagata tccaataatg aatgctaatt atctagagtc gacctgcagg agcttggcgt
    14581 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    14641 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    14701 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    14761 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggagcttgt tgtaactgaa
    14821 aaaggaaaat tattgtgcca ggcagttgaa agtcagcacc ttttaacgag tgctgaaatg
    14881 acggctaaat gggaaacgta ttt
//
