LOCUS       40924_12620        7613 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian vector pCOIN-DV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7613)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7613)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    35..277
                     /label=chicken beta-globin 5'HS4 insulator core sequence
                     /note="chicken beta-globin 5'HS4 insulator core sequence"
     misc_feature    278..520
                     /label=chicken beta-globin 5'HS4 insulator core sequence
                     /note="chicken beta-globin 5'HS4 insulator core sequence"
     promoter        546..1045
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     regulatory      1059..1068
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1070..1117
                     /label=FRT mutant
                     /note="FRT mutant"
     primer_bind     complement(1162..1178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1186..1202)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1210..1240)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1255..1276)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1564..2152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2326..3183)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3184..3288)
                     /label=AmpR promoter
     primer_bind     3762..3778
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3801..3848
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    3865..3968
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     4001..4017
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4031..4064
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4773..4907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(5005..5101)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      5005..5101
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    5027..5161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(5259..5355)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      5259..5355
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    5281..5415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(5444..5477)
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(5444..5477)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     5552..5568
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5575..5699
                     /label=attR4
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5743..6045)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(6390..7046)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7100..7130)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(7155..7278)
                     /label=attR3
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7286..7302)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7344..7568
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca
       61 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
      121 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc
      181 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
      241 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc
      301 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
      361 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
      421 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
      481 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt
      541 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
      601 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
      661 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
      721 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
      781 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
      841 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
      901 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
      961 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     1021 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat
     1081 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca
     1141 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3361 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     3541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     3601 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3661 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3721 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3781 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata
     3841 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc
     3901 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc
     3961 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg
     4021 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg
     4081 caggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     4141 gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     4201 ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     4261 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     4321 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     4381 ggtaggatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc
     4441 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt
     4501 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca
     4561 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat
     4621 cagaagcttg cagatctgcg actctagagg atctgcgact ctagaggatc ataatcagcc
     4681 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     4741 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     4801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc gactctagag
     4921 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     4981 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     5161 ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     5221 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     5281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5401 tatcatgtct ggatccccat caagctgatc cggaaccctt aatataactt cgtataatgt
     5461 atgctatacg aagttattag gtccctcgac ctgcagccca agctagctta tcgataccgt
     5521 cgacnctcga gnttttccca gtcacgacgt tgtaaaacga cggccagtga attatcaact
     5581 ttgtatagaa aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     5641 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
     5701 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag
     5761 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat
     5821 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat
     5881 atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg cactggccag
     5941 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag
     6001 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt
     6061 tctcgtcggc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct
     6121 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc
     6181 ttaccgaacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc
     6241 aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac
     6301 atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg
     6361 gcttttagta agccggatcc tctagattac gccccgccct gccactcatc gcagtactgt
     6421 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     6481 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     6541 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     6601 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     6661 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     6721 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     6781 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga
     6841 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     6901 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     6961 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     7021 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga
     7081 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7141 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     7201 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa
     7261 ctttattata catagttgat aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7321 acgagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7381 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     7441 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     7501 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     7561 ctctatggct tctgaggcgg aaagaaccag ctggggctcg atcctctagt tgg
//
